P52195 (CMA1_PAPHA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chymase
EC=3.4.21.39

Alternative name(s):
Alpha-chymase
Mast cell chymase

Gene names

Name:	CMA1
Synonyms:	CHM

Organism

Papio hamadryas (Hamadryas baboon)

Taxonomic identifier

9557 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Cercopithecidae › Cercopithecinae › Papio

Protein attributes

Sequence length	247 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion.
Catalytic activity	Preferential cleavage: Phe-|-Xaa > Tyr-|-Xaa > Trp-|-Xaa > Leu-|-Xaa.
Subcellular location	Secreted. Cytoplasmic granule. Note: Mast cell granules.
Sequence similarities	Belongs to the peptidase S1 family. Granzyme subfamily. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Glycoprotein Zymogen
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	By similarity
	Propeptide	20 – 21	2	Activation peptide		PRO_0000027437
	Chain	22 – 247	226	Chymase		PRO_0000027438
Regions
	Domain	22 – 245	224	Peptidase S1
Sites
	Active site	66	1	Charge relay system By similarity
	Active site	110	1	Charge relay system By similarity
	Active site	203	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	80	1	N-linked (GlcNAc...) Potential
	Glycosylation	103	1	N-linked (GlcNAc...) Potential
	Disulfide bond	51 ↔ 67		By similarity
	Disulfide bond	144 ↔ 209		By similarity
	Disulfide bond	175 ↔ 188		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P52195 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: E0EC15E0FA72FD8B	FASTA	247	27,339
	10 20 30 40 50 60 MLLLPLPLLL LFLCSRAEAG EIIGGTECKP HSRPYMAYLE IVTSNGPSKS CGGFLIRRNF 70 80 90 100 110 120 VLTAAHCAGR SITVTLGAHN ITEKEDTWQE LEVIKQFRHP KYNTSTLHHD IMLLKLKEKA 130 140 150 160 170 180 SLTLAVGTLP FPSQFNFVPP GRMCRVAGWG RTGVLKPGSD TLQEVKLRLM DPQACSHFRY 190 200 210 220 230 240 FDHNLQLCVG NPRKTKSAFK GDSGGPLLCA GVAQGIVSYG RLDAKPPAVF TRISHYRPWI NKILQAN

References

[1]	Liao Y., Karnik S., Husain A. Submitted (OCT-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U38521 mRNA. Translation: AAA91160.1. U38463 Genomic DNA. Translation: AAA91159.1.
3D structure databases
ProteinModelPortal	P52195.
SMR	P52195. Positions 22-247.
ModBase	Search...
Protein family/group databases
MEROPS	S01.140.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG013304.
Family and domain databases
InterPro	IPR009003. Pept_cys/ser_Trypsin-like. IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
SUPFAM	SSF50494. Pept_Ser_Cys. 1 hit.
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CMA1_PAPHA

Accession

Primary (citable) accession number: P52195

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	November 16, 2011
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program