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Protein

Calmegin

Gene

Clgn

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions during spermatogenesis as a chaperone for a range of client proteins that are important for sperm adhesion onto the egg zona pellucida and for subsequent penetration of the zona pellucida. Required for normal sperm migration from the uterus into the oviduct. Required for normal male fertility. Binds calcium ions.4 Publications

GO - Molecular functioni

  • calcium ion binding Source: MGI
  • protein binding involved in protein folding Source: MGI

GO - Biological processi

  • binding of sperm to zona pellucida Source: MGI
  • cell differentiation Source: UniProtKB-KW
  • meiotic cell cycle Source: UniProtKB-KW
  • multicellular organism development Source: UniProtKB-KW
  • protein complex assembly Source: MGI
  • single fertilization Source: MGI
  • spermatogenesis Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Chaperone, Developmental protein

Keywords - Biological processi

Differentiation, Meiosis, Spermatogenesis

Keywords - Ligandi

Calcium

Names & Taxonomyi

Protein namesi
Recommended name:
Calmegin
Alternative name(s):
A2/6
Calnexin-T
MEG 1 antigen
Gene namesi
Name:Clgn
Synonyms:Meg1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:107472. Clgn.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini20 – 471LumenalSequence analysisAdd BLAST452
Transmembranei472 – 492HelicalSequence analysisAdd BLAST21
Topological domaini493 – 611CytoplasmicSequence analysisAdd BLAST119

GO - Cellular componenti

  • endoplasmic reticulum Source: MGI
  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: UniProtKB-KW
  • nuclear envelope Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Disruption phenotypei

Male mice show normal mating behavior and produce morphologically normal sperm, but are nearly sterile. Mutant sperm fail to adhere to the egg zona pellucida and are generally unable to penetrate the egg extracellular matrix. In addition, mutant sperm display defects in migration from the uterus into the oviduct.3 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
ChainiPRO_000000421120 – 611CalmeginAdd BLAST592

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei128N6-acetyllysineBy similarity1
Disulfide bondi151 ↔ 185By similarity
Disulfide bondi351 ↔ 355By similarity
Modified residuei561PhosphoserineBy similarity1
Modified residuei567PhosphothreonineBy similarity1
Modified residuei578PhosphoserineCombined sources1
Modified residuei580PhosphoserineCombined sources1
Modified residuei582PhosphoserineCombined sources1
Modified residuei592PhosphoserineCombined sources1
Modified residuei595PhosphoserineCombined sources1
Modified residuei602PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Disulfide bond, Phosphoprotein

Proteomic databases

MaxQBiP52194.
PaxDbiP52194.
PeptideAtlasiP52194.
PRIDEiP52194.

PTM databases

iPTMnetiP52194.
PhosphoSitePlusiP52194.

Expressioni

Tissue specificityi

Detected in testis (at protein level). Detected in testis.5 Publications

Developmental stagei

Specifically expressed during male meiotic germ cell development. First detected in early pachytene spermatocytes. Expression is highest in elongating and round spermatids and decreases thereafter. Not detectable in mature spermatids.2 Publications

Gene expression databases

BgeeiENSMUSG00000002190.
CleanExiMM_CLGN.
GenevisibleiP52194. MM.

Interactioni

Subunit structurei

Interacts with PDILT and PPIB (By similarity). Interacts with ADAM2. Interacts with ADAM1A, ADAM1B and ADAM3; these are protein-coding genes in mouse but may be pseudogenes in other organisms.By similarity1 Publication

GO - Molecular functioni

  • protein binding involved in protein folding Source: MGI

Protein-protein interaction databases

DIPiDIP-47581N.
IntActiP52194. 2 interactors.
MINTiMINT-4592857.
STRINGi10090.ENSMUSP00000002259.

Structurei

3D structure databases

ProteinModelPortaliP52194.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati267 – 2801-1Add BLAST14
Repeati284 – 2971-2Add BLAST14
Repeati303 – 3161-3Add BLAST14
Repeati322 – 3351-4Add BLAST14
Repeati339 – 3522-1Add BLAST14
Repeati356 – 3692-2Add BLAST14
Repeati370 – 3832-3Add BLAST14
Repeati384 – 3972-4Add BLAST14

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni317 – 350Interaction with PPIBBy similarityAdd BLAST34

Sequence similaritiesi

Belongs to the calreticulin family.Curated

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0675. Eukaryota.
ENOG410XP7T. LUCA.
GeneTreeiENSGT00430000030841.
HOGENOMiHOG000192436.
HOVERGENiHBG005407.
InParanoidiP52194.
KOiK09551.
OMAiPACRIGC.
OrthoDBiEOG091G04SS.
TreeFamiTF300618.

Family and domain databases

Gene3Di2.10.250.10. 2 hits.
2.60.120.200. 1 hit.
InterProiIPR001580. Calret/calnex.
IPR018124. Calret/calnex_CS.
IPR009033. Calreticulin/calnexin_P_dom.
IPR013320. ConA-like_dom.
[Graphical view]
PANTHERiPTHR11073. PTHR11073. 2 hits.
PfamiPF00262. Calreticulin. 1 hit.
[Graphical view]
PRINTSiPR00626. CALRETICULIN.
SUPFAMiSSF49899. SSF49899. 1 hit.
SSF63887. SSF63887. 1 hit.
PROSITEiPS00803. CALRETICULIN_1. 1 hit.
PS00804. CALRETICULIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P52194-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRFQGVGLCL GLLFITVNAD FMDDGVEVED FSENSDESNI KDEPSSGTFK
60 70 80 90 100
YKTPQPIGEV YFTETFDSGN LAGWVLSKAK KDDMDSEIAI YDGRWEIEEL
110 120 130 140 150
KENQVPGDRG LVLKSKAKHH AIAAVLEKPF IFADKPLIVQ YEVNFQDGID
160 170 180 190 200
CGGAYIKLLA DTGDLILENF YDKTSYTIMF GPDKCGEDYK LHLIFRHKHP
210 220 230 240 250
KTGVFEEKHA KPPDVDLKEF FTDRKTHLYT LVMNPDDTFE VLIDQKVVNQ
260 270 280 290 300
GTLLDDVVPP INPPREIDDP SDKKPEEWDD RAKIPDPTAV RPEDWDENEP
310 320 330 340 350
AQIEDSSAVK PDGWLDDEPK FIPNPKAEKP EDWSDDMDGE WEAPHIPNPA
360 370 380 390 400
CQIGCGEWKP PMIDNPKYKG IWRPPMINNP NYQGLWSPQK IPNPDYFEDD
410 420 430 440 450
HPFLLTSFSA LGLELWSMTP DIYFDNFIIC SEKEVADQWA TDGWELKIMV
460 470 480 490 500
ANANEPGVLR QLVIAAEERP WLWLMYLVMA GLPVALVASF CWPRKVKKKY
510 520 530 540 550
EDTGPKKTEL CKLQSKAALE QEAEEEKAPE KPEDVQEEKK PGEAEVVTVE
560 570 580 590 600
KEVIGEPEEK SKEDRETLEG QEEVSKLSKS GSEDEMKDAD ESPGSGDAPL
610
KSLRKRRVRK D
Length:611
Mass (Da):69,431
Last modified:July 27, 2011 - v2
Checksum:iD70A3C418229DA00
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti76 – 79LSKA → YQS in AAA20599 (PubMed:8188695).Curated4
Sequence conflicti87E → Q in AAA20599 (PubMed:8188695).Curated1
Sequence conflicti127E → D in AAA20599 (PubMed:8188695).Curated1
Sequence conflicti133 – 135ADK → GAIN in AAA20599 (PubMed:8188695).Curated3
Sequence conflicti201K → Q in AAA20599 (PubMed:8188695).Curated1
Sequence conflicti210A → P in AAA20599 (PubMed:8188695).Curated1
Sequence conflicti291R → K in BAA03180 (PubMed:8126001).Curated1
Sequence conflicti291R → K in AAA20599 (PubMed:8188695).Curated1
Sequence conflicti291R → K in BAA22591 (PubMed:9434179).Curated1
Sequence conflicti350A → D in AAA20599 (PubMed:8188695).Curated1
Sequence conflicti432E → D in AAA20599 (PubMed:8188695).Curated1
Sequence conflicti436A → P in AAA20599 (PubMed:8188695).Curated1
Sequence conflicti440A → P in AAA20599 (PubMed:8188695).Curated1
Sequence conflicti445E → D in AAA20599 (PubMed:8188695).Curated1
Sequence conflicti451A → T in AAA20599 (PubMed:8188695).Curated1
Sequence conflicti522E → D in AAA20599 (PubMed:8188695).Curated1
Sequence conflicti549V → L in AAA20599 (PubMed:8188695).Curated1
Sequence conflicti577 – 578LS → AE in BAB31782 (PubMed:16141072).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D14117 mRNA. Translation: BAA03180.1.
U08373 mRNA. Translation: AAA20599.1.
D86323 mRNA. Translation: BAA22591.1.
AK019534 mRNA. Translation: BAB31782.1. Sequence problems.
BC050767 mRNA. Translation: AAH50767.1.
CCDSiCCDS22452.1.
PIRiA53418.
A54086.
RefSeqiNP_001316556.1. NM_001329627.1.
NP_034034.2. NM_009904.4.
XP_011246600.1. XM_011248298.2.
UniGeneiMm.358581.
Mm.426481.

Genome annotation databases

EnsembliENSMUST00000002259; ENSMUSP00000002259; ENSMUSG00000002190.
ENSMUST00000109831; ENSMUSP00000105457; ENSMUSG00000002190.
GeneIDi12745.
KEGGimmu:12745.
UCSCiuc009mkd.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D14117 mRNA. Translation: BAA03180.1.
U08373 mRNA. Translation: AAA20599.1.
D86323 mRNA. Translation: BAA22591.1.
AK019534 mRNA. Translation: BAB31782.1. Sequence problems.
BC050767 mRNA. Translation: AAH50767.1.
CCDSiCCDS22452.1.
PIRiA53418.
A54086.
RefSeqiNP_001316556.1. NM_001329627.1.
NP_034034.2. NM_009904.4.
XP_011246600.1. XM_011248298.2.
UniGeneiMm.358581.
Mm.426481.

3D structure databases

ProteinModelPortaliP52194.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-47581N.
IntActiP52194. 2 interactors.
MINTiMINT-4592857.
STRINGi10090.ENSMUSP00000002259.

PTM databases

iPTMnetiP52194.
PhosphoSitePlusiP52194.

Proteomic databases

MaxQBiP52194.
PaxDbiP52194.
PeptideAtlasiP52194.
PRIDEiP52194.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000002259; ENSMUSP00000002259; ENSMUSG00000002190.
ENSMUST00000109831; ENSMUSP00000105457; ENSMUSG00000002190.
GeneIDi12745.
KEGGimmu:12745.
UCSCiuc009mkd.1. mouse.

Organism-specific databases

CTDi1047.
MGIiMGI:107472. Clgn.

Phylogenomic databases

eggNOGiKOG0675. Eukaryota.
ENOG410XP7T. LUCA.
GeneTreeiENSGT00430000030841.
HOGENOMiHOG000192436.
HOVERGENiHBG005407.
InParanoidiP52194.
KOiK09551.
OMAiPACRIGC.
OrthoDBiEOG091G04SS.
TreeFamiTF300618.

Miscellaneous databases

PROiP52194.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000002190.
CleanExiMM_CLGN.
GenevisibleiP52194. MM.

Family and domain databases

Gene3Di2.10.250.10. 2 hits.
2.60.120.200. 1 hit.
InterProiIPR001580. Calret/calnex.
IPR018124. Calret/calnex_CS.
IPR009033. Calreticulin/calnexin_P_dom.
IPR013320. ConA-like_dom.
[Graphical view]
PANTHERiPTHR11073. PTHR11073. 2 hits.
PfamiPF00262. Calreticulin. 1 hit.
[Graphical view]
PRINTSiPR00626. CALRETICULIN.
SUPFAMiSSF49899. SSF49899. 1 hit.
SSF63887. SSF63887. 1 hit.
PROSITEiPS00803. CALRETICULIN_1. 1 hit.
PS00804. CALRETICULIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCLGN_MOUSE
AccessioniPrimary (citable) accession number: P52194
Secondary accession number(s): Q80YU3, Q9D2K5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: July 27, 2011
Last modified: November 30, 2016
This is version 148 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.