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Protein

Calmegin

Gene

Clgn

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions during spermatogenesis as a chaperone for a range of client proteins that are important for sperm adhesion onto the egg zona pellucida and for subsequent penetration of the zona pellucida. Required for normal sperm migration from the uterus into the oviduct. Required for normal male fertility. Binds calcium ions.4 Publications

GO - Molecular functioni

  • calcium ion binding Source: MGI
  • protein binding involved in protein folding Source: MGI

GO - Biological processi

  • binding of sperm to zona pellucida Source: MGI
  • cell differentiation Source: UniProtKB-KW
  • meiotic cell cycle Source: UniProtKB-KW
  • multicellular organism development Source: UniProtKB-KW
  • protein complex assembly Source: MGI
  • single fertilization Source: MGI
  • spermatogenesis Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Chaperone, Developmental protein

Keywords - Biological processi

Differentiation, Meiosis, Spermatogenesis

Keywords - Ligandi

Calcium

Names & Taxonomyi

Protein namesi
Recommended name:
Calmegin
Alternative name(s):
A2/6
Calnexin-T
MEG 1 antigen
Gene namesi
Name:Clgn
Synonyms:Meg1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:107472. Clgn.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini20 – 471452LumenalSequence analysisAdd
BLAST
Transmembranei472 – 49221HelicalSequence analysisAdd
BLAST
Topological domaini493 – 611119CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • endoplasmic reticulum Source: MGI
  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: UniProtKB-KW
  • nuclear envelope Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Disruption phenotypei

Male mice show normal mating behavior and produce morphologically normal sperm, but are nearly sterile. Mutant sperm fail to adhere to the egg zona pellucida and are generally unable to penetrate the egg extracellular matrix. In addition, mutant sperm display defects in migration from the uterus into the oviduct.3 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Sequence analysisAdd
BLAST
Chaini20 – 611592CalmeginPRO_0000004211Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei128 – 1281N6-acetyllysineBy similarity
Disulfide bondi151 ↔ 185By similarity
Disulfide bondi351 ↔ 355By similarity
Modified residuei561 – 5611PhosphoserineBy similarity
Modified residuei567 – 5671PhosphothreonineBy similarity
Modified residuei578 – 5781PhosphoserineCombined sources
Modified residuei580 – 5801PhosphoserineCombined sources
Modified residuei582 – 5821PhosphoserineCombined sources
Modified residuei592 – 5921PhosphoserineCombined sources
Modified residuei595 – 5951PhosphoserineCombined sources
Modified residuei602 – 6021PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Disulfide bond, Phosphoprotein

Proteomic databases

MaxQBiP52194.
PaxDbiP52194.
PRIDEiP52194.

PTM databases

iPTMnetiP52194.
PhosphoSiteiP52194.

Expressioni

Tissue specificityi

Detected in testis (at protein level). Detected in testis.5 Publications

Developmental stagei

Specifically expressed during male meiotic germ cell development. First detected in early pachytene spermatocytes. Expression is highest in elongating and round spermatids and decreases thereafter. Not detectable in mature spermatids.2 Publications

Gene expression databases

BgeeiP52194.
CleanExiMM_CLGN.
GenevisibleiP52194. MM.

Interactioni

Subunit structurei

Interacts with PDILT and PPIB (By similarity). Interacts with ADAM2. Interacts with ADAM1A, ADAM1B and ADAM3; these are protein-coding genes in mouse but may be pseudogenes in other organisms.By similarity1 Publication

GO - Molecular functioni

  • protein binding involved in protein folding Source: MGI

Protein-protein interaction databases

DIPiDIP-47581N.
IntActiP52194. 2 interactions.
MINTiMINT-4592857.
STRINGi10090.ENSMUSP00000002259.

Structurei

3D structure databases

ProteinModelPortaliP52194.
SMRiP52194. Positions 51-446.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati267 – 280141-1Add
BLAST
Repeati284 – 297141-2Add
BLAST
Repeati303 – 316141-3Add
BLAST
Repeati322 – 335141-4Add
BLAST
Repeati339 – 352142-1Add
BLAST
Repeati356 – 369142-2Add
BLAST
Repeati370 – 383142-3Add
BLAST
Repeati384 – 397142-4Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni317 – 35034Interaction with PPIBBy similarityAdd
BLAST

Sequence similaritiesi

Belongs to the calreticulin family.Curated

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0675. Eukaryota.
ENOG410XP7T. LUCA.
GeneTreeiENSGT00430000030841.
HOGENOMiHOG000192436.
HOVERGENiHBG005407.
InParanoidiP52194.
KOiK09551.
OMAiPACRIGC.
OrthoDBiEOG77126Z.
TreeFamiTF300618.

Family and domain databases

Gene3Di2.10.250.10. 2 hits.
2.60.120.200. 1 hit.
InterProiIPR001580. Calret/calnex.
IPR018124. Calret/calnex_CS.
IPR009033. Calreticulin/calnexin_P_dom.
IPR013320. ConA-like_dom.
[Graphical view]
PANTHERiPTHR11073. PTHR11073. 2 hits.
PfamiPF00262. Calreticulin. 1 hit.
[Graphical view]
PRINTSiPR00626. CALRETICULIN.
SUPFAMiSSF49899. SSF49899. 1 hit.
SSF63887. SSF63887. 1 hit.
PROSITEiPS00803. CALRETICULIN_1. 1 hit.
PS00804. CALRETICULIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P52194-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRFQGVGLCL GLLFITVNAD FMDDGVEVED FSENSDESNI KDEPSSGTFK
60 70 80 90 100
YKTPQPIGEV YFTETFDSGN LAGWVLSKAK KDDMDSEIAI YDGRWEIEEL
110 120 130 140 150
KENQVPGDRG LVLKSKAKHH AIAAVLEKPF IFADKPLIVQ YEVNFQDGID
160 170 180 190 200
CGGAYIKLLA DTGDLILENF YDKTSYTIMF GPDKCGEDYK LHLIFRHKHP
210 220 230 240 250
KTGVFEEKHA KPPDVDLKEF FTDRKTHLYT LVMNPDDTFE VLIDQKVVNQ
260 270 280 290 300
GTLLDDVVPP INPPREIDDP SDKKPEEWDD RAKIPDPTAV RPEDWDENEP
310 320 330 340 350
AQIEDSSAVK PDGWLDDEPK FIPNPKAEKP EDWSDDMDGE WEAPHIPNPA
360 370 380 390 400
CQIGCGEWKP PMIDNPKYKG IWRPPMINNP NYQGLWSPQK IPNPDYFEDD
410 420 430 440 450
HPFLLTSFSA LGLELWSMTP DIYFDNFIIC SEKEVADQWA TDGWELKIMV
460 470 480 490 500
ANANEPGVLR QLVIAAEERP WLWLMYLVMA GLPVALVASF CWPRKVKKKY
510 520 530 540 550
EDTGPKKTEL CKLQSKAALE QEAEEEKAPE KPEDVQEEKK PGEAEVVTVE
560 570 580 590 600
KEVIGEPEEK SKEDRETLEG QEEVSKLSKS GSEDEMKDAD ESPGSGDAPL
610
KSLRKRRVRK D
Length:611
Mass (Da):69,431
Last modified:July 27, 2011 - v2
Checksum:iD70A3C418229DA00
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti76 – 794LSKA → YQS in AAA20599 (PubMed:8188695).Curated
Sequence conflicti87 – 871E → Q in AAA20599 (PubMed:8188695).Curated
Sequence conflicti127 – 1271E → D in AAA20599 (PubMed:8188695).Curated
Sequence conflicti133 – 1353ADK → GAIN in AAA20599 (PubMed:8188695).Curated
Sequence conflicti201 – 2011K → Q in AAA20599 (PubMed:8188695).Curated
Sequence conflicti210 – 2101A → P in AAA20599 (PubMed:8188695).Curated
Sequence conflicti291 – 2911R → K in BAA03180 (PubMed:8126001).Curated
Sequence conflicti291 – 2911R → K in AAA20599 (PubMed:8188695).Curated
Sequence conflicti291 – 2911R → K in BAA22591 (PubMed:9434179).Curated
Sequence conflicti350 – 3501A → D in AAA20599 (PubMed:8188695).Curated
Sequence conflicti432 – 4321E → D in AAA20599 (PubMed:8188695).Curated
Sequence conflicti436 – 4361A → P in AAA20599 (PubMed:8188695).Curated
Sequence conflicti440 – 4401A → P in AAA20599 (PubMed:8188695).Curated
Sequence conflicti445 – 4451E → D in AAA20599 (PubMed:8188695).Curated
Sequence conflicti451 – 4511A → T in AAA20599 (PubMed:8188695).Curated
Sequence conflicti522 – 5221E → D in AAA20599 (PubMed:8188695).Curated
Sequence conflicti549 – 5491V → L in AAA20599 (PubMed:8188695).Curated
Sequence conflicti577 – 5782LS → AE in BAB31782 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D14117 mRNA. Translation: BAA03180.1.
U08373 mRNA. Translation: AAA20599.1.
D86323 mRNA. Translation: BAA22591.1.
AK019534 mRNA. Translation: BAB31782.1. Sequence problems.
BC050767 mRNA. Translation: AAH50767.1.
CCDSiCCDS22452.1.
PIRiA53418.
A54086.
RefSeqiNP_034034.2. NM_009904.2.
XP_006530698.1. XM_006530635.1.
XP_011246600.1. XM_011248298.1.
UniGeneiMm.358581.

Genome annotation databases

EnsembliENSMUST00000002259; ENSMUSP00000002259; ENSMUSG00000002190.
ENSMUST00000109831; ENSMUSP00000105457; ENSMUSG00000002190.
GeneIDi12745.
KEGGimmu:12745.
UCSCiuc009mkd.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D14117 mRNA. Translation: BAA03180.1.
U08373 mRNA. Translation: AAA20599.1.
D86323 mRNA. Translation: BAA22591.1.
AK019534 mRNA. Translation: BAB31782.1. Sequence problems.
BC050767 mRNA. Translation: AAH50767.1.
CCDSiCCDS22452.1.
PIRiA53418.
A54086.
RefSeqiNP_034034.2. NM_009904.2.
XP_006530698.1. XM_006530635.1.
XP_011246600.1. XM_011248298.1.
UniGeneiMm.358581.

3D structure databases

ProteinModelPortaliP52194.
SMRiP52194. Positions 51-446.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-47581N.
IntActiP52194. 2 interactions.
MINTiMINT-4592857.
STRINGi10090.ENSMUSP00000002259.

PTM databases

iPTMnetiP52194.
PhosphoSiteiP52194.

Proteomic databases

MaxQBiP52194.
PaxDbiP52194.
PRIDEiP52194.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000002259; ENSMUSP00000002259; ENSMUSG00000002190.
ENSMUST00000109831; ENSMUSP00000105457; ENSMUSG00000002190.
GeneIDi12745.
KEGGimmu:12745.
UCSCiuc009mkd.1. mouse.

Organism-specific databases

CTDi1047.
MGIiMGI:107472. Clgn.

Phylogenomic databases

eggNOGiKOG0675. Eukaryota.
ENOG410XP7T. LUCA.
GeneTreeiENSGT00430000030841.
HOGENOMiHOG000192436.
HOVERGENiHBG005407.
InParanoidiP52194.
KOiK09551.
OMAiPACRIGC.
OrthoDBiEOG77126Z.
TreeFamiTF300618.

Miscellaneous databases

NextBioi282066.
PROiP52194.
SOURCEiSearch...

Gene expression databases

BgeeiP52194.
CleanExiMM_CLGN.
GenevisibleiP52194. MM.

Family and domain databases

Gene3Di2.10.250.10. 2 hits.
2.60.120.200. 1 hit.
InterProiIPR001580. Calret/calnex.
IPR018124. Calret/calnex_CS.
IPR009033. Calreticulin/calnexin_P_dom.
IPR013320. ConA-like_dom.
[Graphical view]
PANTHERiPTHR11073. PTHR11073. 2 hits.
PfamiPF00262. Calreticulin. 1 hit.
[Graphical view]
PRINTSiPR00626. CALRETICULIN.
SUPFAMiSSF49899. SSF49899. 1 hit.
SSF63887. SSF63887. 1 hit.
PROSITEiPS00803. CALRETICULIN_1. 1 hit.
PS00804. CALRETICULIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of a novel Ca(2+)-binding protein (calmegin) specifically expressed during male meiotic germ cell development."
    Watanabe D., Yamada K., Nishina Y., Tajima Y., Koshimizu U., Nagata A., Nishimune Y.
    J. Biol. Chem. 269:7744-7749(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], CALCIUM-BINDING, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.
    Strain: ICR.
    Tissue: Testis.
  2. "Molecular cloning and sequencing of calnexin-t. An abundant male germ cell-specific calcium-binding protein of the endoplasmic reticulum."
    Ohsako S., Hayashi Y., Bunick D.
    J. Biol. Chem. 269:14140-14148(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], CALCIUM-BINDING, TISSUE SPECIFICITY.
    Strain: CD-1.
    Tissue: Testis.
  3. "Cloning and characterization of the human Calmegin gene encoding putative testis-specific chaperone."
    Tanaka H., Ikawa M., Tsuchida J., Nozaki M., Suzuki M., Fujiwara T., Okabe M., Nishimune Y.
    Gene 204:159-163(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Strain: C57BL/6J.
    Tissue: Testis.
  4. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Testis.
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.
  6. "The putative chaperone calmegin is required for sperm fertility."
    Ikawa M., Wada I., Kominami K., Watanabe D., Toshimori K., Nishimune Y., Okabe M.
    Nature 387:607-611(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE, FUNCTION, TISSUE SPECIFICITY.
  7. "Molecular chaperone calmegin localization to the endoplasmic reticulum of meiotic and post-meiotic germ cells in the mouse testis."
    Yoshinaga K., Tanii I., Toshimori K.
    Arch. Histol. Cytol. 62:283-293(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.
  8. "Calmegin is required for fertilin alpha/beta heterodimerization and sperm fertility."
    Ikawa M., Nakanishi T., Yamada S., Wada I., Kominami K., Tanaka H., Nozaki M., Nishimune Y., Okabe M.
    Dev. Biol. 240:254-261(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE, INTERACTION WITH ADAM1B AND ADAM2, TISSUE SPECIFICITY.
  9. "Selective passage through the uterotubal junction of sperm from a mixed population produced by chimeras of calmegin-knockout and wild-type male mice."
    Nakanishi T., Isotani A., Yamaguchi R., Ikawa M., Baba T., Suarez S.S., Okabe M.
    Biol. Reprod. 71:959-965(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  10. "Aberrant distribution of ADAM3 in sperm from both angiotensin-converting enzyme (Ace)- and calmegin (Clgn)-deficient mice."
    Yamaguchi R., Yamagata K., Ikawa M., Moss S.B., Okabe M.
    Biol. Reprod. 75:760-766(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  11. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-578; SER-580; SER-582; SER-592 AND SER-595, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain and Testis.

Entry informationi

Entry nameiCLGN_MOUSE
AccessioniPrimary (citable) accession number: P52194
Secondary accession number(s): Q80YU3, Q9D2K5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: July 27, 2011
Last modified: May 11, 2016
This is version 142 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.