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P52193

- CALR_BOVIN

UniProt

P52193 - CALR_BOVIN

Protein

Calreticulin

Gene

CALR

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 113 (01 Oct 2014)
      Sequence version 2 (22 Feb 2003)
      Previous versions | rss
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    Functioni

    Calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export. Involved in maternal gene expression regulation. May participate in oocyte maturation via the regulation of calcium homeostasis By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi26 – 261Calcium; via carbonyl oxygenBy similarity
    Metal bindingi62 – 621Calcium; via carbonyl oxygenBy similarity
    Metal bindingi64 – 641Calcium; via carbonyl oxygenBy similarity
    Binding sitei109 – 1091CarbohydrateBy similarity
    Binding sitei111 – 1111CarbohydrateBy similarity
    Binding sitei128 – 1281CarbohydrateBy similarity
    Binding sitei135 – 1351CarbohydrateBy similarity
    Binding sitei317 – 3171CarbohydrateBy similarity
    Metal bindingi328 – 3281CalciumBy similarity

    GO - Molecular functioni

    1. calcium ion binding Source: UniProtKB

    GO - Biological processi

    1. protein folding Source: InterPro
    2. protein stabilization Source: UniProtKB

    Keywords - Molecular functioni

    Chaperone

    Keywords - Ligandi

    Calcium, Lectin, Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Calreticulin
    Alternative name(s):
    CRP55
    Calregulin
    HACBP
    Gene namesi
    Name:CALR
    Synonyms:CRT
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Unplaced

    Subcellular locationi

    Endoplasmic reticulum lumen 1 PublicationPROSITE-ProRule annotation. Sarcoplasmic reticulum lumen By similarity

    GO - Cellular componenti

    1. sarcoplasmic reticulum lumen Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Endoplasmic reticulum, Sarcoplasmic reticulum

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 17172 PublicationsAdd
    BLAST
    Chaini18 – 417400CalreticulinPRO_0000004170Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei48 – 481N6-acetyllysineBy similarity
    Disulfide bondi137 ↔ 163
    Modified residuei159 – 1591N6-acetyllysineBy similarity
    Glycosylationi179 – 1791N-linked (GlcNAc...)
    Modified residuei209 – 2091N6-acetyllysineBy similarity

    Keywords - PTMi

    Acetylation, Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiP52193.
    PRIDEiP52193.

    Interactioni

    Subunit structurei

    Monomer. Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5. Interacts with GABARAP, NR3C1, PDIA3/ERp57 and TRIM21. Interacts with PPIB By similarity.By similarity

    Protein-protein interaction databases

    IntActiP52193. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliP52193.
    SMRiP52193. Positions 206-305.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati191 – 202121-1Add
    BLAST
    Repeati210 – 221121-2Add
    BLAST
    Repeati227 – 238121-3Add
    BLAST
    Repeati244 – 255121-4Add
    BLAST
    Repeati259 – 269112-1Add
    BLAST
    Repeati273 – 283112-2Add
    BLAST
    Repeati287 – 297112-3Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni18 – 197180N-domainAdd
    BLAST
    Regioni191 – 255654 X approximate repeatsAdd
    BLAST
    Regioni198 – 308111P-domainAdd
    BLAST
    Regioni237 – 27034Interaction with PPIBBy similarityAdd
    BLAST
    Regioni259 – 297393 X approximate repeatsAdd
    BLAST
    Regioni309 – 417109C-domainAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi414 – 4174Prevents secretion from ERPROSITE-ProRule annotation

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi351 – 40757Asp/Glu/Lys-richAdd
    BLAST

    Domaini

    Can be divided into a N-terminal globular domain, a proline-rich P-domain forming an elongated arm-like structure and a C-terminal acidic domain. The P-domain binds one molecule of calcium with high affinity, whereas the acidic C-domain binds multiple calcium ions with low affinity By similarity.By similarity
    The interaction with glycans occurs through a binding site in the globular lectin domain.By similarity
    The zinc binding sites are localized to the N-domain.By similarity
    Associates with PDIA3 through the tip of the extended arm formed by the P-domain.By similarity

    Sequence similaritiesi

    Belongs to the calreticulin family.Curated

    Keywords - Domaini

    Repeat, Signal

    Phylogenomic databases

    eggNOGiNOG305105.
    HOGENOMiHOG000192435.
    HOVERGENiHBG005407.
    InParanoidiP52193.
    KOiK08057.

    Family and domain databases

    Gene3Di2.60.120.200. 2 hits.
    InterProiIPR001580. Calret/calnex.
    IPR018124. Calret/calnex_CS.
    IPR009169. Calreticulin.
    IPR009033. Calreticulin/calnexin_P_dom.
    IPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    [Graphical view]
    PANTHERiPTHR11073. PTHR11073. 1 hit.
    PfamiPF00262. Calreticulin. 1 hit.
    [Graphical view]
    PIRSFiPIRSF002356. Calreticulin. 1 hit.
    PRINTSiPR00626. CALRETICULIN.
    SUPFAMiSSF49899. SSF49899. 2 hits.
    SSF63887. SSF63887. 1 hit.
    PROSITEiPS00803. CALRETICULIN_1. 1 hit.
    PS00804. CALRETICULIN_2. 1 hit.
    PS00805. CALRETICULIN_REPEAT. 3 hits.
    PS00014. ER_TARGET. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P52193-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLLPVPLLLG LLGLAAADPT VYFKEQFLDG DGWTERWIES KHKPDFGKFV    50
    LSSGKFYGDQ EKDKGLQTSQ DARFYALSAR FEPFSNKGQT LVVQFTVKHE 100
    QNIDCGGGYV KLFPAGLDQT DMHGDSEYNI MFGPDICGPG TKKVHVIFNY 150
    KGKNVLINKD IRCKDDEFTH LYTLIVRPNN TYEVKIDNSQ VESGSLEDDW 200
    DFLPPKKIKD PDAAKPEDWD DRAKIDDPTD SKPEDWDKPE HIPDPDAKKP 250
    EDWDEEMDGE WEPPVIQNPE YKGEWKPRQI DNPEYKGIWI HPEIDNPEYS 300
    PDSNIYAYEN FAVLGLDLWQ VKSGTIFDNF LITNDEAYAE EFGNETWGVT 350
    KAAEKQMKDK QDEEQRLHEE EEEKKGKEEE EADKDDDEDK DEDEEDEDEK 400
    EEEEEEDAAA GQAKDEL 417
    Length:417
    Mass (Da):48,039
    Last modified:February 22, 2003 - v2
    Checksum:i7BF812C7B5417BE9
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti1 – 9999MLLPV…FTVKH → MCLNHFLLSLVLSIVLLFHF VFYICLHHIVTFLREETVFF SEQFLTLDLKYKASKLSSIR EALSMSKVGIIENFCFSEIS FLQESIKSHGRRTLVGCSPW GHE in AAC37307. (PubMed:8373827)CuratedAdd
    BLAST
    Sequence conflicti18 – 214DPTV → EPAI AA sequence (PubMed:2016321)Curated
    Sequence conflicti111 – 1122KL → NV in AAC37307. (PubMed:8373827)Curated
    Sequence conflicti265 – 2651V → L in AAC37307. (PubMed:8373827)Curated
    Sequence conflicti383 – 3831D → E in AAC37307. (PubMed:8373827)Curated
    Sequence conflicti411 – 4111G → A in AAC37307. (PubMed:8373827)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L13462 mRNA. Translation: AAC37307.1.
    AB067687 mRNA. Translation: BAB86913.1.
    PIRiA33208.
    S36799.
    S43376.
    RefSeqiNP_776425.1. NM_174000.2.
    UniGeneiBt.30105.

    Genome annotation databases

    GeneIDi281036.
    KEGGibta:281036.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L13462 mRNA. Translation: AAC37307.1 .
    AB067687 mRNA. Translation: BAB86913.1 .
    PIRi A33208.
    S36799.
    S43376.
    RefSeqi NP_776425.1. NM_174000.2.
    UniGenei Bt.30105.

    3D structure databases

    ProteinModelPortali P52193.
    SMRi P52193. Positions 206-305.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P52193. 1 interaction.

    Proteomic databases

    PaxDbi P52193.
    PRIDEi P52193.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 281036.
    KEGGi bta:281036.

    Organism-specific databases

    CTDi 811.

    Phylogenomic databases

    eggNOGi NOG305105.
    HOGENOMi HOG000192435.
    HOVERGENi HBG005407.
    InParanoidi P52193.
    KOi K08057.

    Miscellaneous databases

    NextBioi 20805123.

    Family and domain databases

    Gene3Di 2.60.120.200. 2 hits.
    InterProi IPR001580. Calret/calnex.
    IPR018124. Calret/calnex_CS.
    IPR009169. Calreticulin.
    IPR009033. Calreticulin/calnexin_P_dom.
    IPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    [Graphical view ]
    PANTHERi PTHR11073. PTHR11073. 1 hit.
    Pfami PF00262. Calreticulin. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF002356. Calreticulin. 1 hit.
    PRINTSi PR00626. CALRETICULIN.
    SUPFAMi SSF49899. SSF49899. 2 hits.
    SSF63887. SSF63887. 1 hit.
    PROSITEi PS00803. CALRETICULIN_1. 1 hit.
    PS00804. CALRETICULIN_2. 1 hit.
    PS00805. CALRETICULIN_REPEAT. 3 hits.
    PS00014. ER_TARGET. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Comparison of cDNAs from bovine brain coding for two isoforms of calreticulin."
      Liu N., Fine R.E., Johnson R.J.
      Biochim. Biophys. Acta 1202:70-76(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Brain.
    2. "Bovine brain calreticulin."
      Hossain M.A., Takuwa K., Minakata H., Nakajima T.
      Submitted (AUG-2001) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Brain.
    3. "Covalent structure of bovine brain calreticulin."
      Matsuoka K., Seta K., Yamakawa Y., Okuyama T., Shinoda T., Isobe T.
      Biochem. J. 298:435-442(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 18-417.
      Tissue: Brain.
    4. "Calreticulin, and not calsequestrin, is the major calcium binding protein of smooth muscle sarcoplasmic reticulum and liver endoplasmic reticulum."
      Milner R.E., Baksh S., Shemanko C., Carpenter M.R., Smillie L., Vance J.E., Opas M., Michalak M.
      J. Biol. Chem. 266:7155-7165(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 18-30.
      Tissue: Liver.
    5. "Calreticulin -- an endoplasmic reticulum protein with calcium-binding activity is also found in the extracellular matrix."
      Somogyi E., Petersson U., Hultenby K., Wendel M.
      Matrix Biol. 22:179-191(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: PARTIAL PROTEIN SEQUENCE, SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiCALR_BOVIN
    AccessioniPrimary (citable) accession number: P52193
    Secondary accession number(s): P28489, P42918, Q8SQ53
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: February 22, 2003
    Last modified: October 1, 2014
    This is version 113 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3