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Protein

Inward rectifier potassium channel 16

Gene

Kcnj16

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. KCNJ16 may be involved in the regulation of fluid and pH balance (By similarity). In the kidney, together with KCNJ10, mediates basolateral K+ recycling in distal tubules; this process is critical for Na+ reabsorption at the tubules.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei161 – 1611Role in the control of polyamine-mediated channel gating and in the blocking by intracellular magnesiumBy similarity

GO - Molecular functioni

GO - Biological processi

  • cellular potassium ion transport Source: RGD
  • potassium ion import Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Potassium transport, Transport

Keywords - Ligandi

Potassium

Names & Taxonomyi

Protein namesi
Recommended name:
Inward rectifier potassium channel 16
Alternative name(s):
BIR9
Inward rectifier K(+) channel Kir5.1
Potassium channel, inwardly rectifying subfamily J member 16
Gene namesi
Name:Kcnj16
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi61824. Kcnj16.

Subcellular locationi

  • Membrane By similarity; Multi-pass membrane protein By similarity
  • Basolateral cell membrane By similarity

  • Note: In kidney distal convoluted tubules, located in the basolateral membrane in the presence of KCNJ10.By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 7070CytoplasmicBy similarityAdd
BLAST
Transmembranei71 – 9525Helical; Name=M1By similarityAdd
BLAST
Topological domaini96 – 11722ExtracellularBy similarityAdd
BLAST
Intramembranei118 – 12912Helical; Pore-forming; Name=H5By similarityAdd
BLAST
Intramembranei130 – 1367Pore-formingBy similarity
Topological domaini137 – 1459ExtracellularBy similarity
Transmembranei146 – 16722Helical; Name=M2By similarityAdd
BLAST
Topological domaini168 – 419252CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Chemistry

GuidetoPHARMACOLOGYi440.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 419419Inward rectifier potassium channel 16PRO_0000154977Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei358 – 3581PhosphoserineBy similarity
Modified residuei374 – 3741PhosphoserineBy similarity
Modified residuei376 – 3761PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP52191.

PTM databases

iPTMnetiP52191.
PhosphoSiteiP52191.

Expressioni

Tissue specificityi

Expressed in the brain, testis, liver, spleen, kidney, submaxillary gland and adrenals. In the kidney, expressed in the epithelial cells of both proximal and distal convoluted tubules, in the endothelial cells surrounding glomerular capillaries and in the flattened parietal layer of Bowman's capsule.1 Publication

Interactioni

Subunit structurei

Heterodimer with Kir4.1/KCNJ10; this interaction is required for KCNJ16 localization to the basolateral membrane in kidney cells. As a heterodimer with KCNJ10, may interact with MAGI1; this interaction may facilitate KCNJ10/KCNJ16 potassium channel expression at the basolateral membrane in kidney cells. May form heterodimers with Kir2.1/KCNJ2.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000006238.

Structurei

3D structure databases

ProteinModelPortaliP52191.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi131 – 1366Selectivity filterBy similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3827. Eukaryota.
ENOG410XQ62. LUCA.
HOGENOMiHOG000237325.
HOVERGENiHBG006178.
InParanoidiP52191.
PhylomeDBiP52191.

Family and domain databases

Gene3Di2.60.40.1400. 1 hit.
InterProiIPR014756. Ig_E-set.
IPR016449. K_chnl_inward-rec_Kir.
IPR008061. K_chnl_inward-rec_Kir5.
IPR013518. K_chnl_inward-rec_Kir_cyto.
[Graphical view]
PANTHERiPTHR11767. PTHR11767. 1 hit.
PTHR11767:SF24. PTHR11767:SF24. 1 hit.
PfamiPF01007. IRK. 1 hit.
[Graphical view]
PIRSFiPIRSF005465. GIRK_kir. 1 hit.
PRINTSiPR01678. KIR5CHANNEL.
PR01320. KIRCHANNEL.
SUPFAMiSSF81296. SSF81296. 1 hit.

Sequencei

Sequence statusi: Complete.

P52191-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSYYGSSYRI VNVDSKYPGY PPEHAIAEKR RARRRLLHKD GSCNVYFKHI
60 70 80 90 100
FGEWGSYMVD IFTTLVDTKW RHMFVVFSLS YILSWLIFGS IFWLIALHHG
110 120 130 140 150
DLLSDPDITP CVDNVHSFTA AFLFSLETQT TIGYGYRCVT EECSVAVLTV
160 170 180 190 200
ILQSILSCII NTFIIGAALA KMATARKRAQ TIRFSYFALI GMRDGKLCLM
210 220 230 240 250
WRIGDFRPNH VVEGTVRAQL LRYSEDSEGR MTMAFKDLKL VNDQIILVTP
260 270 280 290 300
VTIVHEIDHE SPLYALDRKA VAKDNFEILV TFIYTGDSTG TSHQSRSSYV
310 320 330 340 350
PREILWGHRF HDVLEVKRKY YKVNCLQFEG SVEVYAPFCS AKQLDWKDQQ
360 370 380 390 400
LNNLEKTSPA RGSCTSDTNT RRRSFSAVAM VSSCENPEET SLSPQDECKE
410
VPYQKALLTL NRISMESQM
Length:419
Mass (Da):47,936
Last modified:April 27, 2001 - v2
Checksum:iACE54A72D511A11C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti361 – 41959RGSCT…MESQM → PRILHLGHQHQEEILQRSCH GEQL in CAA58564 (PubMed:7874445).CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X83581 mRNA. Translation: CAA58564.1.
AF249676 Genomic DNA. Translation: AAF74265.1.
UniGeneiRn.1989.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X83581 mRNA. Translation: CAA58564.1.
AF249676 Genomic DNA. Translation: AAF74265.1.
UniGeneiRn.1989.

3D structure databases

ProteinModelPortaliP52191.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000006238.

Chemistry

GuidetoPHARMACOLOGYi440.

PTM databases

iPTMnetiP52191.
PhosphoSiteiP52191.

Proteomic databases

PaxDbiP52191.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

RGDi61824. Kcnj16.

Phylogenomic databases

eggNOGiKOG3827. Eukaryota.
ENOG410XQ62. LUCA.
HOGENOMiHOG000237325.
HOVERGENiHBG006178.
InParanoidiP52191.
PhylomeDBiP52191.

Miscellaneous databases

PROiP52191.

Family and domain databases

Gene3Di2.60.40.1400. 1 hit.
InterProiIPR014756. Ig_E-set.
IPR016449. K_chnl_inward-rec_Kir.
IPR008061. K_chnl_inward-rec_Kir5.
IPR013518. K_chnl_inward-rec_Kir_cyto.
[Graphical view]
PANTHERiPTHR11767. PTHR11767. 1 hit.
PTHR11767:SF24. PTHR11767:SF24. 1 hit.
PfamiPF01007. IRK. 1 hit.
[Graphical view]
PIRSFiPIRSF005465. GIRK_kir. 1 hit.
PRINTSiPR01678. KIR5CHANNEL.
PR01320. KIRCHANNEL.
SUPFAMiSSF81296. SSF81296. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiKCJ16_RAT
AccessioniPrimary (citable) accession number: P52191
Secondary accession number(s): Q9JI87
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: April 27, 2001
Last modified: July 6, 2016
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.