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P52191 (IRK16_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Inward rectifier potassium channel 16
Alternative name(s):
BIR9
Inward rectifier K(+) channel Kir5.1
Potassium channel, inwardly rectifying subfamily J member 16
Gene names
Name:Kcnj16
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length419 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. KCNJ16 may be involved in the regulation of fluid and pH balance By similarity.

Subunit structure

Seems to form heterodimer with Kir4.1/KCNJ10 or Kir2.1/KCNJ2.

Subcellular location

Membrane; Multi-pass membrane protein.

Tissue specificity

In the brain, testis, liver, spleen, kidney, submaxillary gland and adrenals.

Sequence similarities

Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ16 subfamily. [View classification]

Ontologies

Keywords
   Biological processIon transport
Potassium transport
Transport
   Cellular componentMembrane
   DomainTransmembrane
Transmembrane helix
   LigandPotassium
   Molecular functionIon channel
Voltage-gated channel
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcellular potassium ion transport

Inferred from direct assay PubMed 19665991. Source: RGD

   Cellular_componentintegral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functioninward rectifier potassium channel activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 419419Inward rectifier potassium channel 16
PRO_0000154977

Regions

Topological domain1 – 7070Cytoplasmic By similarity
Transmembrane71 – 9525Helical; Name=M1; By similarity
Topological domain96 – 11722Extracellular By similarity
Intramembrane118 – 12912Helical; Pore-forming; Name=H5; By similarity
Intramembrane130 – 1367Pore-forming; By similarity
Topological domain137 – 1459Extracellular By similarity
Transmembrane146 – 16722Helical; Name=M2; By similarity
Topological domain168 – 419252Cytoplasmic By similarity
Motif131 – 1366Selectivity filter By similarity

Sites

Site1611Role in the control of polyamine-mediated channel gating and in the blocking by intracellular magnesium By similarity

Experimental info

Sequence conflict361 – 41959RGSCT…MESQM → PRILHLGHQHQEEILQRSCH GEQL in CAA58564. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P52191 [UniParc].

Last modified April 27, 2001. Version 2.
Checksum: ACE54A72D511A11C

FASTA41947,936
        10         20         30         40         50         60 
MSYYGSSYRI VNVDSKYPGY PPEHAIAEKR RARRRLLHKD GSCNVYFKHI FGEWGSYMVD 

        70         80         90        100        110        120 
IFTTLVDTKW RHMFVVFSLS YILSWLIFGS IFWLIALHHG DLLSDPDITP CVDNVHSFTA 

       130        140        150        160        170        180 
AFLFSLETQT TIGYGYRCVT EECSVAVLTV ILQSILSCII NTFIIGAALA KMATARKRAQ 

       190        200        210        220        230        240 
TIRFSYFALI GMRDGKLCLM WRIGDFRPNH VVEGTVRAQL LRYSEDSEGR MTMAFKDLKL 

       250        260        270        280        290        300 
VNDQIILVTP VTIVHEIDHE SPLYALDRKA VAKDNFEILV TFIYTGDSTG TSHQSRSSYV 

       310        320        330        340        350        360 
PREILWGHRF HDVLEVKRKY YKVNCLQFEG SVEVYAPFCS AKQLDWKDQQ LNNLEKTSPA 

       370        380        390        400        410 
RGSCTSDTNT RRRSFSAVAM VSSCENPEET SLSPQDECKE VPYQKALLTL NRISMESQM 

« Hide

References

[1]"Cloning and expression of a family of inward rectifier potassium channels."
Bond C.T., Pessia M., Xia X.-M., Lagrutta A., Kavanaugh M.P., Adelman J.P.
Recept. Channels 2:183-191(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Sprague-Dawley.
Tissue: Brain.
[2]"pH dependence of the inwardly rectifying potassium channel, Kir5.1, and localization in renal tubular epithelia."
Tucker S.J., Imbrici P., Salvatore L., D'Adamo M.C., Pessia M.
J. Biol. Chem. 275:16404-16407(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X83581 mRNA. Translation: CAA58564.1.
AF249676 Genomic DNA. Translation: AAF74265.1.
UniGeneRn.1989.

3D structure databases

ProteinModelPortalP52191.
ModBaseSearch...
MobiDBSearch...

Chemistry

GuidetoPHARMACOLOGY440.

PTM databases

PhosphoSiteP52191.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

RGD61824. Kcnj16.

Phylogenomic databases

eggNOGNOG277052.
HOGENOMHOG000237325.
HOVERGENHBG006178.
InParanoidP52191.
PhylomeDBP52191.

Gene expression databases

GenevestigatorP52191.

Family and domain databases

Gene3D2.60.40.1400. 1 hit.
InterProIPR014756. Ig_E-set.
IPR016449. K_chnl_inward-rec_Kir.
IPR008061. K_chnl_inward-rec_Kir5.
IPR013518. K_chnl_inward-rec_Kir_cyto.
[Graphical view]
PANTHERPTHR11767. PTHR11767. 1 hit.
PTHR11767:SF24. PTHR11767:SF24. 1 hit.
PfamPF01007. IRK. 1 hit.
[Graphical view]
PIRSFPIRSF005465. GIRK_kir. 1 hit.
PRINTSPR01678. KIR5CHANNEL.
PR01320. KIRCHANNEL.
SUPFAMSSF81296. SSF81296. 1 hit.
ProtoNetSearch...

Other

PROP52191.

Entry information

Entry nameIRK16_RAT
AccessionPrimary (citable) accession number: P52191
Secondary accession number(s): Q9JI87
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: April 27, 2001
Last modified: April 16, 2014
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families