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P52190 (IRK4_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 114. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Inward rectifier potassium channel 4
Alternative name(s):
BIR11
Brain inwardly rectifying K(+) channel 2
Inward rectifier K(+) channel Kir2.3
Short name=IRK-3
Potassium channel, inwardly rectifying subfamily J member 4
Gene names
Name:Kcnj4
Synonyms:Irk3
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length446 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

This receptor is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium and cesium. Ref.4

Subunit structure

Homomultimeric and heteromultimeric association with Kir2.1, resulting in an enhanced G-protein-induced current. Interacts with TAX1BP3. TAX1BP3 competes with LIN7 family members for KCNJ4 binding By similarity. Association, via its PDZ-recognition domain, with LIN7A, LIN7B, LIN7C, DLG1, CASK and APBA1 plays a key role in its localization and trafficking. Ref.4

Subcellular location

Cell membrane; Multi-pass membrane protein. Cytoplasmic vesicle membrane By similarity. Note: TAX1BP3 binding promotes dissociation of KCNJ4 from LIN7 famaly members and KCNJ4 internalization By similarity.

Tissue specificity

Detected in kidney distal convoluted tubules (at protein level). Widely expressed throughout the brain. Also found in some peripheral tissues. Ref.5

Sequence similarities

Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ4 subfamily. [View classification]

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 446446Inward rectifier potassium channel 4
PRO_0000154933

Regions

Topological domain1 – 5555Cytoplasmic By similarity
Transmembrane56 – 8025Helical; Name=M1; By similarity
Topological domain81 – 12040Extracellular By similarity
Intramembrane121 – 13212Helical; Pore-forming; Name=H5; By similarity
Intramembrane133 – 1397Pore-forming; By similarity
Topological domain140 – 1489Extracellular By similarity
Transmembrane149 – 17022Helical; Name=M2; By similarity
Topological domain171 – 446276Cytoplasmic By similarity
Region91 – 11121Val/Gly/Ala/Pro stretch
Motif134 – 1396Selectivity filter By similarity
Motif444 – 4463PDZ-binding Potential
Compositional bias362 – 3687Poly-Pro
Compositional bias383 – 3908Poly-Glu
Compositional bias391 – 3999Poly-Ala

Sites

Site1641Role in the control of polyamine-mediated channel gating and in the blocking by intracellular magnesium By similarity

Experimental info

Sequence conflict53 – 542Missing in AAA87812. Ref.3
Sequence conflict91 – 988PSGPTAGG → LRAHGGS in AAA87812. Ref.3
Sequence conflict1091T → R in AAA87812. Ref.3
Sequence conflict115 – 1228IMHVNGFL → YHACKRLFW in AAA87812. Ref.3
Sequence conflict130 – 1312ET → GA in AAA87812. Ref.3
Sequence conflict1351I → Y in AAA87812. Ref.3
Sequence conflict1551Missing in AAA87812. Ref.3
Sequence conflict1761P → A in AAA87812. Ref.3
Sequence conflict1761P → G Ref.2
Sequence conflict1781P → S Ref.2
Sequence conflict197 – 1982DG → T in AAA87812. Ref.3
Sequence conflict205 – 2073RVG → GWV in AAA87812. Ref.3
Sequence conflict2941V → A in AAA87812. Ref.3
Sequence conflict2981A → V in CAA60963. Ref.2
Sequence conflict3081L → Q in AAA87812. Ref.3

Sequences

Sequence LengthMass (Da)Tools
P52190 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: A86073996861D20C

FASTA44649,690
        10         20         30         40         50         60 
MHGHSRNGQA HVPRRKRRNR FVKKNGQCNV YFANLSNKSQ RYMADIFTTC VDTRWRYMLM 

        70         80         90        100        110        120 
IFSAAFLVSW LFFGLLFWCI AFFHGDLEPS PSGPTAGGPG GNGGGAAPTA AKPCIMHVNG 

       130        140        150        160        170        180 
FLGAFLFSVE TQTTIGYGFR CVTEECPLAV IAVVVQSIVG CVIDSFMIGT IMAKMPRPKK 

       190        200        210        220        230        240 
RAQTLLFSHH AVISVRDGKL CLMWRVGNLR KSHIVEAHVR AQLIKPYMTQ EGEYLPLDQR 

       250        260        270        280        290        300 
DLNVGYDIGL DRIFLVSPII IVHEIDEDSP LYGMGKEELE SEDFEIVVIL EGMVEATAMT 

       310        320        330        340        350        360 
TQARSSYLAS EILWGHRFEP VVFEEKSHYK VDYSRFHKTY EVAGTPCCSA RELQESKITV 

       370        380        390        400        410        420 
LPAPPPPPSA FCYENELALM SQEEEEMEEE AAAAAAVAAG LGLEAGSKEE TGIIRMLEFG 

       430        440 
SHLDLERMQA ATLPLDNISY RRESAI 

« Hide

References

[1]"Cloning, functional expression and mRNA distribution of an inwardly rectifying potassium channel protein."
Falk T., Meyerhof W., Corrette B.J., Schaefer J., Bauer C.K., Schwarz J.R., Richter D.
FEBS Lett. 367:127-131(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: GH3/B6.
[2]"Cloning and expression of a family of inward rectifier potassium channels."
Bond C.T., Pessia M., Xia X.-M., Lagrutta A., Kavanaugh M.P., Adelman J.P.
Recept. Channels 2:183-191(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Sprague-Dawley.
Tissue: Brain.
[3]"Cloning and expression of two brain-specific inwardly rectifying potassium channels."
Bredt D.S., Wang T.L., Cohen N.A., Guggino W.B., Snyder S.H.
Proc. Natl. Acad. Sci. U.S.A. 92:6753-6757(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]"A multiprotein trafficking complex composed of SAP97, CASK, Veli, and Mint1 is associated with inward rectifier Kir2 potassium channels."
Leonoudakis D., Conti L.R., Radeke C.M., McGuire L.M., Vandenberg C.A.
J. Biol. Chem. 279:19051-19063(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH CASK; LIN7A; LIN7B; LIN7C; APBA1 AND DLG1, FUNCTION.
[5]"TIP-1 has PDZ scaffold antagonist activity."
Alewine C., Olsen O., Wade J.B., Welling P.A.
Mol. Biol. Cell 17:4200-4211(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X87635 mRNA. Translation: CAA60963.1.
X83580 mRNA. Translation: CAA58563.1.
U27582 mRNA. Translation: AAA87812.1.
PIRS66268.
RefSeqNP_446322.2. NM_053870.2.
UniGeneRn.10197.

3D structure databases

ProteinModelPortalP52190.
SMRP52190. Positions 179-357.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteP52190.

Proteomic databases

PaxDbP52190.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID116649.
KEGGrno:116649.
UCSCRGD:621436. rat.

Organism-specific databases

CTD3761.
RGD621436. Kcnj4.

Phylogenomic databases

eggNOGNOG72812.
HOGENOMHOG000237325.
HOVERGENHBG006178.
InParanoidP52190.
KOK04998.
PhylomeDBP52190.

Gene expression databases

GenevestigatorP52190.

Family and domain databases

Gene3D2.60.40.1400. 1 hit.
InterProIPR014756. Ig_E-set.
IPR016449. K_chnl_inward-rec_Kir.
IPR003273. K_chnl_inward-rec_Kir2.3.
IPR013518. K_chnl_inward-rec_Kir_cyto.
[Graphical view]
PANTHERPTHR11767. PTHR11767. 1 hit.
PTHR11767:SF13. PTHR11767:SF13. 1 hit.
PfamPF01007. IRK. 1 hit.
[Graphical view]
PIRSFPIRSF005465. GIRK_kir. 1 hit.
PRINTSPR01326. KIR23CHANNEL.
PR01320. KIRCHANNEL.
SUPFAMSSF81296. SSF81296. 1 hit.
ProtoNetSearch...

Other

NextBio619399.
PROP52190.

Entry information

Entry nameIRK4_RAT
AccessionPrimary (citable) accession number: P52190
Secondary accession number(s): O35752
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 16, 2014
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families