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P52190

- KCNJ4_RAT

UniProt

P52190 - KCNJ4_RAT

Protein

Inward rectifier potassium channel 4

Gene

Kcnj4

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 117 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium and cesium By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei164 – 1641Role in the control of polyamine-mediated channel gating and in the blocking by intracellular magnesiumBy similarity

    GO - Molecular functioni

    1. inward rectifier potassium channel activity Source: InterPro

    Keywords - Molecular functioni

    Ion channel, Voltage-gated channel

    Keywords - Biological processi

    Ion transport, Potassium transport, Transport

    Keywords - Ligandi

    Potassium

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Inward rectifier potassium channel 4
    Alternative name(s):
    BIR11
    Brain inwardly rectifying K(+) channel 2
    Inward rectifier K(+) channel Kir2.3
    Short name:
    IRK-3
    Potassium channel, inwardly rectifying subfamily J member 4
    Gene namesi
    Name:Kcnj4
    Synonyms:Irk3
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi621436. Kcnj4.

    Subcellular locationi

    Cell membrane; Multi-pass membrane protein. Cytoplasmic vesicle membrane By similarity. Cell junctionsynapsepostsynaptic cell membrane By similarity; Multi-pass membrane protein By similarity
    Note: TAX1BP3 binding promotes dissociation of KCNJ4 from LIN7 famaly members and KCNJ4 internalization.By similarity

    GO - Cellular componenti

    1. cell junction Source: UniProtKB-KW
    2. cytoplasmic vesicle membrane Source: UniProtKB-SubCell
    3. dendrite Source: RGD
    4. integral component of membrane Source: UniProtKB-KW
    5. neuronal cell body Source: RGD
    6. postsynaptic membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell junction, Cell membrane, Cytoplasmic vesicle, Membrane, Postsynaptic cell membrane, Synapse

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 446446Inward rectifier potassium channel 4PRO_0000154933Add
    BLAST

    Proteomic databases

    PaxDbiP52190.

    PTM databases

    PhosphoSiteiP52190.

    Expressioni

    Tissue specificityi

    Detected in kidney distal convoluted tubules (at protein level). Widely expressed throughout the brain. Also found in some peripheral tissues.1 Publication

    Gene expression databases

    GenevestigatoriP52190.

    Interactioni

    Subunit structurei

    Homomultimeric and heteromultimeric association with KCNJ2 and KCNJ12. Association, via its PDZ-recognition domain, with LIN7A, LIN7B, LIN7C, DLG1, CASK and APBA1 plays a key role in its localization and trafficking. Interacts with TAX1BP3. TAX1BP3 competes with LIN7 family members for KCNJ4 binding.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliP52190.
    SMRiP52190. Positions 179-357.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 5555CytoplasmicBy similarityAdd
    BLAST
    Topological domaini81 – 12040ExtracellularBy similarityAdd
    BLAST
    Topological domaini140 – 1489ExtracellularBy similarity
    Topological domaini171 – 446276CytoplasmicBy similarityAdd
    BLAST

    Intramembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Intramembranei121 – 13212Helical; Pore-forming; Name=H5By similarityAdd
    BLAST
    Intramembranei133 – 1397Pore-formingBy similarity

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei56 – 8025Helical; Name=M1By similarityAdd
    BLAST
    Transmembranei149 – 17022Helical; Name=M2By similarityAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni91 – 11121Val/Gly/Ala/Pro stretchAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi134 – 1396Selectivity filterBy similarity
    Motifi444 – 4463PDZ-bindingSequence Analysis

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi362 – 3687Poly-Pro
    Compositional biasi383 – 3908Poly-Glu
    Compositional biasi391 – 3999Poly-Ala

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG72812.
    HOGENOMiHOG000237325.
    HOVERGENiHBG006178.
    InParanoidiP52190.
    KOiK04998.
    PhylomeDBiP52190.

    Family and domain databases

    Gene3Di2.60.40.1400. 1 hit.
    InterProiIPR014756. Ig_E-set.
    IPR016449. K_chnl_inward-rec_Kir.
    IPR003273. K_chnl_inward-rec_Kir2.3.
    IPR013518. K_chnl_inward-rec_Kir_cyto.
    [Graphical view]
    PANTHERiPTHR11767. PTHR11767. 1 hit.
    PTHR11767:SF13. PTHR11767:SF13. 1 hit.
    PfamiPF01007. IRK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005465. GIRK_kir. 1 hit.
    PRINTSiPR01326. KIR23CHANNEL.
    PR01320. KIRCHANNEL.
    SUPFAMiSSF81296. SSF81296. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P52190-1 [UniParc]FASTAAdd to Basket

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    MHGHSRNGQA HVPRRKRRNR FVKKNGQCNV YFANLSNKSQ RYMADIFTTC    50
    VDTRWRYMLM IFSAAFLVSW LFFGLLFWCI AFFHGDLEPS PSGPTAGGPG 100
    GNGGGAAPTA AKPCIMHVNG FLGAFLFSVE TQTTIGYGFR CVTEECPLAV 150
    IAVVVQSIVG CVIDSFMIGT IMAKMPRPKK RAQTLLFSHH AVISVRDGKL 200
    CLMWRVGNLR KSHIVEAHVR AQLIKPYMTQ EGEYLPLDQR DLNVGYDIGL 250
    DRIFLVSPII IVHEIDEDSP LYGMGKEELE SEDFEIVVIL EGMVEATAMT 300
    TQARSSYLAS EILWGHRFEP VVFEEKSHYK VDYSRFHKTY EVAGTPCCSA 350
    RELQESKITV LPAPPPPPSA FCYENELALM SQEEEEMEEE AAAAAAVAAG 400
    LGLEAGSKEE TGIIRMLEFG SHLDLERMQA ATLPLDNISY RRESAI 446
    Length:446
    Mass (Da):49,690
    Last modified:October 1, 1996 - v1
    Checksum:iA86073996861D20C
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti53 – 542Missing in AAA87812. (PubMed:7624316)Curated
    Sequence conflicti91 – 988PSGPTAGG → LRAHGGS in AAA87812. (PubMed:7624316)Curated
    Sequence conflicti109 – 1091T → R in AAA87812. (PubMed:7624316)Curated
    Sequence conflicti115 – 1228IMHVNGFL → YHACKRLFW in AAA87812. (PubMed:7624316)Curated
    Sequence conflicti130 – 1312ET → GA in AAA87812. (PubMed:7624316)Curated
    Sequence conflicti135 – 1351I → Y in AAA87812. (PubMed:7624316)Curated
    Sequence conflicti155 – 1551Missing in AAA87812. (PubMed:7624316)Curated
    Sequence conflicti176 – 1761P → A in AAA87812. (PubMed:7624316)Curated
    Sequence conflicti176 – 1761P → G(PubMed:7874445)Curated
    Sequence conflicti178 – 1781P → S(PubMed:7874445)Curated
    Sequence conflicti197 – 1982DG → T in AAA87812. (PubMed:7624316)Curated
    Sequence conflicti205 – 2073RVG → GWV in AAA87812. (PubMed:7624316)Curated
    Sequence conflicti294 – 2941V → A in AAA87812. (PubMed:7624316)Curated
    Sequence conflicti298 – 2981A → V in CAA60963. (PubMed:7874445)Curated
    Sequence conflicti308 – 3081L → Q in AAA87812. (PubMed:7624316)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X87635 mRNA. Translation: CAA60963.1.
    X83580 mRNA. Translation: CAA58563.1.
    U27582 mRNA. Translation: AAA87812.1.
    PIRiS66268.
    RefSeqiNP_446322.2. NM_053870.2.
    UniGeneiRn.10197.

    Genome annotation databases

    GeneIDi116649.
    KEGGirno:116649.
    UCSCiRGD:621436. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X87635 mRNA. Translation: CAA60963.1 .
    X83580 mRNA. Translation: CAA58563.1 .
    U27582 mRNA. Translation: AAA87812.1 .
    PIRi S66268.
    RefSeqi NP_446322.2. NM_053870.2.
    UniGenei Rn.10197.

    3D structure databases

    ProteinModelPortali P52190.
    SMRi P52190. Positions 179-357.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei P52190.

    Proteomic databases

    PaxDbi P52190.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 116649.
    KEGGi rno:116649.
    UCSCi RGD:621436. rat.

    Organism-specific databases

    CTDi 3761.
    RGDi 621436. Kcnj4.

    Phylogenomic databases

    eggNOGi NOG72812.
    HOGENOMi HOG000237325.
    HOVERGENi HBG006178.
    InParanoidi P52190.
    KOi K04998.
    PhylomeDBi P52190.

    Miscellaneous databases

    NextBioi 619399.
    PROi P52190.

    Gene expression databases

    Genevestigatori P52190.

    Family and domain databases

    Gene3Di 2.60.40.1400. 1 hit.
    InterProi IPR014756. Ig_E-set.
    IPR016449. K_chnl_inward-rec_Kir.
    IPR003273. K_chnl_inward-rec_Kir2.3.
    IPR013518. K_chnl_inward-rec_Kir_cyto.
    [Graphical view ]
    PANTHERi PTHR11767. PTHR11767. 1 hit.
    PTHR11767:SF13. PTHR11767:SF13. 1 hit.
    Pfami PF01007. IRK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005465. GIRK_kir. 1 hit.
    PRINTSi PR01326. KIR23CHANNEL.
    PR01320. KIRCHANNEL.
    SUPFAMi SSF81296. SSF81296. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Cloning, functional expression and mRNA distribution of an inwardly rectifying potassium channel protein."
      Falk T., Meyerhof W., Corrette B.J., Schaefer J., Bauer C.K., Schwarz J.R., Richter D.
      FEBS Lett. 367:127-131(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: GH3/B6.
    2. "Cloning and expression of a family of inward rectifier potassium channels."
      Bond C.T., Pessia M., Xia X.-M., Lagrutta A., Kavanaugh M.P., Adelman J.P.
      Recept. Channels 2:183-191(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Sprague-Dawley.
      Tissue: Brain.
    3. "Cloning and expression of two brain-specific inwardly rectifying potassium channels."
      Bredt D.S., Wang T.L., Cohen N.A., Guggino W.B., Snyder S.H.
      Proc. Natl. Acad. Sci. U.S.A. 92:6753-6757(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    4. "A multiprotein trafficking complex composed of SAP97, CASK, Veli, and Mint1 is associated with inward rectifier Kir2 potassium channels."
      Leonoudakis D., Conti L.R., Radeke C.M., McGuire L.M., Vandenberg C.A.
      J. Biol. Chem. 279:19051-19063(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CASK; LIN7A; LIN7B; LIN7C; APBA1 AND DLG1, FUNCTION.
    5. Cited for: TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiKCNJ4_RAT
    AccessioniPrimary (citable) accession number: P52190
    Secondary accession number(s): O35752
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 117 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3