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Protein

Inward rectifier potassium channel 4

Gene

Kcnj4

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium and cesium (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei164 – 1641Role in the control of polyamine-mediated channel gating and in the blocking by intracellular magnesiumBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Potassium transport, Transport

Keywords - Ligandi

Potassium

Names & Taxonomyi

Protein namesi
Recommended name:
Inward rectifier potassium channel 4
Alternative name(s):
BIR11
Brain inwardly rectifying K(+) channel 2
Inward rectifier K(+) channel Kir2.3
Short name:
IRK-3
Potassium channel, inwardly rectifying subfamily J member 4
Gene namesi
Name:Kcnj4
Synonyms:Irk3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Unplaced

Organism-specific databases

RGDi621436. Kcnj4.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 5555CytoplasmicBy similarityAdd
BLAST
Transmembranei56 – 8025Helical; Name=M1By similarityAdd
BLAST
Topological domaini81 – 12040ExtracellularBy similarityAdd
BLAST
Intramembranei121 – 13212Helical; Pore-forming; Name=H5By similarityAdd
BLAST
Intramembranei133 – 1397Pore-formingBy similarity
Topological domaini140 – 1489ExtracellularBy similarity
Transmembranei149 – 17022Helical; Name=M2By similarityAdd
BLAST
Topological domaini171 – 446276CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

  • cell junction Source: UniProtKB-KW
  • cytoplasmic vesicle membrane Source: UniProtKB-SubCell
  • dendrite Source: RGD
  • integral component of plasma membrane Source: GO_Central
  • neuronal cell body Source: RGD
  • postsynaptic membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasmic vesicle, Membrane, Postsynaptic cell membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 446446Inward rectifier potassium channel 4PRO_0000154933Add
BLAST

Proteomic databases

PaxDbiP52190.

PTM databases

PhosphoSiteiP52190.

Expressioni

Tissue specificityi

Detected in kidney distal convoluted tubules (at protein level). Widely expressed throughout the brain. Also found in some peripheral tissues.1 Publication

Gene expression databases

GenevisibleiP52190. RN.

Interactioni

Subunit structurei

Homomultimeric and heteromultimeric association with KCNJ2 and KCNJ12. Association, via its PDZ-recognition domain, with LIN7A, LIN7B, LIN7C, DLG1, CASK and APBA1 plays a key role in its localization and trafficking. Interacts with TAX1BP3. TAX1BP3 competes with LIN7 family members for KCNJ4 binding.1 Publication

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000053014.

Structurei

3D structure databases

ProteinModelPortaliP52190.
SMRiP52190. Positions 179-357.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni91 – 11121Val/Gly/Ala/Pro stretchAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi134 – 1396Selectivity filterBy similarity
Motifi444 – 4463PDZ-bindingSequence Analysis

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi362 – 3687Poly-Pro
Compositional biasi383 – 3908Poly-Glu
Compositional biasi391 – 3999Poly-Ala

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG72812.
HOGENOMiHOG000237325.
HOVERGENiHBG006178.
InParanoidiP52190.
KOiK04998.
PhylomeDBiP52190.

Family and domain databases

Gene3Di2.60.40.1400. 1 hit.
InterProiIPR014756. Ig_E-set.
IPR016449. K_chnl_inward-rec_Kir.
IPR003273. K_chnl_inward-rec_Kir2.3.
IPR013518. K_chnl_inward-rec_Kir_cyto.
[Graphical view]
PANTHERiPTHR11767. PTHR11767. 1 hit.
PTHR11767:SF13. PTHR11767:SF13. 1 hit.
PfamiPF01007. IRK. 1 hit.
[Graphical view]
PIRSFiPIRSF005465. GIRK_kir. 1 hit.
PRINTSiPR01326. KIR23CHANNEL.
PR01320. KIRCHANNEL.
SUPFAMiSSF81296. SSF81296. 1 hit.

Sequencei

Sequence statusi: Complete.

P52190-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHGHSRNGQA HVPRRKRRNR FVKKNGQCNV YFANLSNKSQ RYMADIFTTC
60 70 80 90 100
VDTRWRYMLM IFSAAFLVSW LFFGLLFWCI AFFHGDLEPS PSGPTAGGPG
110 120 130 140 150
GNGGGAAPTA AKPCIMHVNG FLGAFLFSVE TQTTIGYGFR CVTEECPLAV
160 170 180 190 200
IAVVVQSIVG CVIDSFMIGT IMAKMPRPKK RAQTLLFSHH AVISVRDGKL
210 220 230 240 250
CLMWRVGNLR KSHIVEAHVR AQLIKPYMTQ EGEYLPLDQR DLNVGYDIGL
260 270 280 290 300
DRIFLVSPII IVHEIDEDSP LYGMGKEELE SEDFEIVVIL EGMVEATAMT
310 320 330 340 350
TQARSSYLAS EILWGHRFEP VVFEEKSHYK VDYSRFHKTY EVAGTPCCSA
360 370 380 390 400
RELQESKITV LPAPPPPPSA FCYENELALM SQEEEEMEEE AAAAAAVAAG
410 420 430 440
LGLEAGSKEE TGIIRMLEFG SHLDLERMQA ATLPLDNISY RRESAI
Length:446
Mass (Da):49,690
Last modified:October 1, 1996 - v1
Checksum:iA86073996861D20C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti53 – 542Missing in AAA87812 (PubMed:7624316).Curated
Sequence conflicti91 – 988PSGPTAGG → LRAHGGS in AAA87812 (PubMed:7624316).Curated
Sequence conflicti109 – 1091T → R in AAA87812 (PubMed:7624316).Curated
Sequence conflicti115 – 1228IMHVNGFL → YHACKRLFW in AAA87812 (PubMed:7624316).Curated
Sequence conflicti130 – 1312ET → GA in AAA87812 (PubMed:7624316).Curated
Sequence conflicti135 – 1351I → Y in AAA87812 (PubMed:7624316).Curated
Sequence conflicti155 – 1551Missing in AAA87812 (PubMed:7624316).Curated
Sequence conflicti176 – 1761P → A in AAA87812 (PubMed:7624316).Curated
Sequence conflicti176 – 1761P → G (PubMed:7874445).Curated
Sequence conflicti178 – 1781P → S (PubMed:7874445).Curated
Sequence conflicti197 – 1982DG → T in AAA87812 (PubMed:7624316).Curated
Sequence conflicti205 – 2073RVG → GWV in AAA87812 (PubMed:7624316).Curated
Sequence conflicti294 – 2941V → A in AAA87812 (PubMed:7624316).Curated
Sequence conflicti298 – 2981A → V in CAA60963 (PubMed:7874445).Curated
Sequence conflicti308 – 3081L → Q in AAA87812 (PubMed:7624316).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X87635 mRNA. Translation: CAA60963.1.
X83580 mRNA. Translation: CAA58563.1.
U27582 mRNA. Translation: AAA87812.1.
PIRiS66268.
RefSeqiNP_446322.2. NM_053870.2.
UniGeneiRn.10197.

Genome annotation databases

GeneIDi116649.
KEGGirno:116649.
UCSCiRGD:621436. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X87635 mRNA. Translation: CAA60963.1.
X83580 mRNA. Translation: CAA58563.1.
U27582 mRNA. Translation: AAA87812.1.
PIRiS66268.
RefSeqiNP_446322.2. NM_053870.2.
UniGeneiRn.10197.

3D structure databases

ProteinModelPortaliP52190.
SMRiP52190. Positions 179-357.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000053014.

PTM databases

PhosphoSiteiP52190.

Proteomic databases

PaxDbiP52190.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi116649.
KEGGirno:116649.
UCSCiRGD:621436. rat.

Organism-specific databases

CTDi3761.
RGDi621436. Kcnj4.

Phylogenomic databases

eggNOGiNOG72812.
HOGENOMiHOG000237325.
HOVERGENiHBG006178.
InParanoidiP52190.
KOiK04998.
PhylomeDBiP52190.

Miscellaneous databases

NextBioi619399.
PROiP52190.

Gene expression databases

GenevisibleiP52190. RN.

Family and domain databases

Gene3Di2.60.40.1400. 1 hit.
InterProiIPR014756. Ig_E-set.
IPR016449. K_chnl_inward-rec_Kir.
IPR003273. K_chnl_inward-rec_Kir2.3.
IPR013518. K_chnl_inward-rec_Kir_cyto.
[Graphical view]
PANTHERiPTHR11767. PTHR11767. 1 hit.
PTHR11767:SF13. PTHR11767:SF13. 1 hit.
PfamiPF01007. IRK. 1 hit.
[Graphical view]
PIRSFiPIRSF005465. GIRK_kir. 1 hit.
PRINTSiPR01326. KIR23CHANNEL.
PR01320. KIRCHANNEL.
SUPFAMiSSF81296. SSF81296. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Cloning, functional expression and mRNA distribution of an inwardly rectifying potassium channel protein."
    Falk T., Meyerhof W., Corrette B.J., Schaefer J., Bauer C.K., Schwarz J.R., Richter D.
    FEBS Lett. 367:127-131(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: GH3/B6.
  2. "Cloning and expression of a family of inward rectifier potassium channels."
    Bond C.T., Pessia M., Xia X.-M., Lagrutta A., Kavanaugh M.P., Adelman J.P.
    Recept. Channels 2:183-191(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Brain.
  3. "Cloning and expression of two brain-specific inwardly rectifying potassium channels."
    Bredt D.S., Wang T.L., Cohen N.A., Guggino W.B., Snyder S.H.
    Proc. Natl. Acad. Sci. U.S.A. 92:6753-6757(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  4. "A multiprotein trafficking complex composed of SAP97, CASK, Veli, and Mint1 is associated with inward rectifier Kir2 potassium channels."
    Leonoudakis D., Conti L.R., Radeke C.M., McGuire L.M., Vandenberg C.A.
    J. Biol. Chem. 279:19051-19063(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CASK; LIN7A; LIN7B; LIN7C; APBA1 AND DLG1, FUNCTION.
  5. Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiKCNJ4_RAT
AccessioniPrimary (citable) accession number: P52190
Secondary accession number(s): O35752
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 24, 2015
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.