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Protein

Inward rectifier potassium channel 4

Gene

Kcnj4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium and cesium (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei163Role in the control of polyamine-mediated channel gating and in the blocking by intracellular magnesiumBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Potassium transport, Transport

Keywords - Ligandi

Potassium

Names & Taxonomyi

Protein namesi
Recommended name:
Inward rectifier potassium channel 4
Alternative name(s):
Inward rectifier K(+) channel Kir2.3
Short name:
IRK-3
Potassium channel, inwardly rectifying subfamily J member 4
Gene namesi
Name:Kcnj4
Synonyms:Irk3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:104743. Kcnj4.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 55CytoplasmicBy similarityAdd BLAST55
Transmembranei56 – 80Helical; Name=M1By similarityAdd BLAST25
Topological domaini81 – 119ExtracellularBy similarityAdd BLAST39
Intramembranei120 – 131Helical; Pore-forming; Name=H5By similarityAdd BLAST12
Intramembranei132 – 138Pore-formingBy similarity7
Topological domaini139 – 147ExtracellularBy similarity9
Transmembranei148 – 169Helical; Name=M2By similarityAdd BLAST22
Topological domaini170 – 445CytoplasmicBy similarityAdd BLAST276

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasmic vesicle, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi442 – 444ESR → AAA: Abolishes interaction with TAX1BP3. 1 Publication3
Mutagenesisi442E → A: Abolishes interaction with TAX1BP3. 1 Publication1
Mutagenesisi443S → A or D: Abolishes interaction with TAX1BP3. 1 Publication1
Mutagenesisi445I → A: Abolishes interaction with TAX1BP3. 1 Publication1

Chemistry databases

GuidetoPHARMACOLOGYi432.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001549321 – 445Inward rectifier potassium channel 4Add BLAST445

Proteomic databases

PaxDbiP52189.
PeptideAtlasiP52189.
PRIDEiP52189.

PTM databases

iPTMnetiP52189.
PhosphoSitePlusiP52189.

Expressioni

Tissue specificityi

Highly expressed in the forebrain, moderately in skeletal muscle. Im olfactory bulb, specifically expressed at the postsynaptic membrane of dendritic spines of granule cells.1 Publication

Developmental stagei

Expression increases between day 2 to 45 and is then stable from day 45 to 120.

Interactioni

Subunit structurei

Homomultimeric and heteromultimeric association with KCNJ2 and KCNJ12. Association, via its PDZ-recognition domain, with LIN7A, LIN7B, LIN7C, DLG1, DLG2, DLG4, CASK and APBA1 plays a key role in its localization and trafficking (By similarity). Interacts with TAX1BP3. TAX1BP3 competes with LIN7 family members for KCNJ4 binding.By similarity2 Publications

GO - Molecular functioni

Protein-protein interaction databases

IntActiP52189. 2 interactors.
MINTiMINT-103836.
STRINGi10090.ENSMUSP00000094075.

Structurei

3D structure databases

ProteinModelPortaliP52189.
SMRiP52189.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi133 – 138Selectivity filterBy similarity6
Motifi443 – 445PDZ-bindingSequence analysis3

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi361 – 367Poly-Pro7
Compositional biasi382 – 389Poly-Glu8
Compositional biasi390 – 398Poly-Ala9

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3827. Eukaryota.
ENOG410XQ62. LUCA.
HOGENOMiHOG000237325.
HOVERGENiHBG006178.
InParanoidiP52189.
PhylomeDBiP52189.

Family and domain databases

Gene3Di2.60.40.1400. 1 hit.
InterProiIPR014756. Ig_E-set.
IPR016449. K_chnl_inward-rec_Kir.
IPR003273. K_chnl_inward-rec_Kir2.3.
IPR013518. K_chnl_inward-rec_Kir_cyto.
[Graphical view]
PANTHERiPTHR11767. PTHR11767. 3 hits.
PfamiPF01007. IRK. 1 hit.
[Graphical view]
PIRSFiPIRSF005465. GIRK_kir. 1 hit.
PRINTSiPR01326. KIR23CHANNEL.
PR01320. KIRCHANNEL.
SUPFAMiSSF81296. SSF81296. 1 hit.

Sequencei

Sequence statusi: Complete.

P52189-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHGHNRNGQA HVPRRKRRNR FVKKNGQCNV YFANLSNKSQ RYMADIFTTC
60 70 80 90 100
VDTRWRYMLM IFSAAFLVSW LFFGLLFWWI AFFHGDLEAS PSVPAVGGPG
110 120 130 140 150
GNGGESPNAP KPCIMHVNGF LGAFLFSVET QTTIGYGFRC VTEECPLAVI
160 170 180 190 200
AVVVQSIVGC VIDSFMIGTI MAKMARPKKR AQTLLFSHHA VISVRDGKLC
210 220 230 240 250
LMWRVGNLRK SHIVEAHVRA QLIKPYMTQE GEYLPLDQRD LNVGYDIGLD
260 270 280 290 300
RIFLVSPIII VHEIDEDSPL YGMGKEELES EDFEIVVILE GMVEATAMTT
310 320 330 340 350
QARSSYLASE ILWGHRFEPV VFEEKSHYKV DYSRFHKTYE VAGTPCCSAR
360 370 380 390 400
ELQESKITVL PAPPPPPSAF CYENELALMS QEEEEMEEEA AAAAAVAAGL
410 420 430 440
GLEAGSKEEA GIIRMLEFGS HLDLERMQAA TLPLDNISYR RESRI
Length:445
Mass (Da):49,899
Last modified:October 1, 1996 - v1
Checksum:i09C9E889CC8E0DF3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti79W → C in AAA53241 (PubMed:7926018).Curated1
Sequence conflicti86D → E in AAA53241 (PubMed:7926018).Curated1
Sequence conflicti96V → A in AAA53241 (PubMed:7926018).Curated1
Sequence conflicti105E → A in AAA53241 (PubMed:7926018).Curated1
Sequence conflicti239R → G in AAA53241 (PubMed:7926018).Curated1
Sequence conflicti444R → A in AAA53241 (PubMed:7926018).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U11075 mRNA. Translation: AAA53241.1.
S71382 mRNA. Translation: AAB31087.1.
CCDSiCCDS27641.1.
PIRiS45713.
UniGeneiMm.140760.
Mm.39759.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U11075 mRNA. Translation: AAA53241.1.
S71382 mRNA. Translation: AAB31087.1.
CCDSiCCDS27641.1.
PIRiS45713.
UniGeneiMm.140760.
Mm.39759.

3D structure databases

ProteinModelPortaliP52189.
SMRiP52189.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP52189. 2 interactors.
MINTiMINT-103836.
STRINGi10090.ENSMUSP00000094075.

Chemistry databases

GuidetoPHARMACOLOGYi432.

PTM databases

iPTMnetiP52189.
PhosphoSitePlusiP52189.

Proteomic databases

PaxDbiP52189.
PeptideAtlasiP52189.
PRIDEiP52189.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

MGIiMGI:104743. Kcnj4.

Phylogenomic databases

eggNOGiKOG3827. Eukaryota.
ENOG410XQ62. LUCA.
HOGENOMiHOG000237325.
HOVERGENiHBG006178.
InParanoidiP52189.
PhylomeDBiP52189.

Miscellaneous databases

PROiP52189.
SOURCEiSearch...

Family and domain databases

Gene3Di2.60.40.1400. 1 hit.
InterProiIPR014756. Ig_E-set.
IPR016449. K_chnl_inward-rec_Kir.
IPR003273. K_chnl_inward-rec_Kir2.3.
IPR013518. K_chnl_inward-rec_Kir_cyto.
[Graphical view]
PANTHERiPTHR11767. PTHR11767. 3 hits.
PfamiPF01007. IRK. 1 hit.
[Graphical view]
PIRSFiPIRSF005465. GIRK_kir. 1 hit.
PRINTSiPR01326. KIR23CHANNEL.
PR01320. KIRCHANNEL.
SUPFAMiSSF81296. SSF81296. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiKCNJ4_MOUSE
AccessioniPrimary (citable) accession number: P52189
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.