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Protein

Inward rectifier potassium channel 4

Gene

Kcnj4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium and cesium (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei163Role in the control of polyamine-mediated channel gating and in the blocking by intracellular magnesiumBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIon channel, Voltage-gated channel
Biological processIon transport, Potassium transport, Transport
LigandPotassium

Names & Taxonomyi

Protein namesi
Recommended name:
Inward rectifier potassium channel 4
Alternative name(s):
Inward rectifier K(+) channel Kir2.3
Short name:
IRK-3
Potassium channel, inwardly rectifying subfamily J member 4
Gene namesi
Name:Kcnj4
Synonyms:Irk3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:104743 Kcnj4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 55CytoplasmicBy similarityAdd BLAST55
Transmembranei56 – 80Helical; Name=M1By similarityAdd BLAST25
Topological domaini81 – 119ExtracellularBy similarityAdd BLAST39
Intramembranei120 – 131Helical; Pore-forming; Name=H5By similarityAdd BLAST12
Intramembranei132 – 138Pore-formingBy similarity7
Topological domaini139 – 147ExtracellularBy similarity9
Transmembranei148 – 169Helical; Name=M2By similarityAdd BLAST22
Topological domaini170 – 445CytoplasmicBy similarityAdd BLAST276

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasmic vesicle, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi442 – 444ESR → AAA: Abolishes interaction with TAX1BP3. 1 Publication3
Mutagenesisi442E → A: Abolishes interaction with TAX1BP3. 1 Publication1
Mutagenesisi443S → A or D: Abolishes interaction with TAX1BP3. 1 Publication1
Mutagenesisi445I → A: Abolishes interaction with TAX1BP3. 1 Publication1

Chemistry databases

GuidetoPHARMACOLOGYi432

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001549321 – 445Inward rectifier potassium channel 4Add BLAST445

Proteomic databases

PaxDbiP52189
PeptideAtlasiP52189
PRIDEiP52189

PTM databases

iPTMnetiP52189
PhosphoSitePlusiP52189
SwissPalmiP52189

Expressioni

Tissue specificityi

Highly expressed in the forebrain, moderately in skeletal muscle. Im olfactory bulb, specifically expressed at the postsynaptic membrane of dendritic spines of granule cells.1 Publication

Developmental stagei

Expression increases between day 2 to 45 and is then stable from day 45 to 120.

Interactioni

Subunit structurei

Homomultimeric and heteromultimeric association with KCNJ2 and KCNJ12. Association, via its PDZ-recognition domain, with LIN7A, LIN7B, LIN7C, DLG1, DLG2, DLG4, CASK and APBA1 plays a key role in its localization and trafficking (By similarity). Interacts with TAX1BP3. TAX1BP3 competes with LIN7 family members for KCNJ4 binding.By similarity2 Publications

GO - Molecular functioni

Protein-protein interaction databases

IntActiP52189, 3 interactors
MINTiP52189
STRINGi10090.ENSMUSP00000094075

Structurei

3D structure databases

ProteinModelPortaliP52189
SMRiP52189
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi133 – 138Selectivity filterBy similarity6
Motifi443 – 445PDZ-bindingSequence analysis3

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi361 – 367Poly-Pro7
Compositional biasi382 – 389Poly-Glu8
Compositional biasi390 – 398Poly-Ala9

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3827 Eukaryota
ENOG410XQ62 LUCA
HOGENOMiHOG000237325
HOVERGENiHBG006178
InParanoidiP52189
PhylomeDBiP52189

Family and domain databases

Gene3Di2.60.40.1400, 2 hits
InterProiView protein in InterPro
IPR014756 Ig_E-set
IPR016449 K_chnl_inward-rec_Kir
IPR003273 K_chnl_inward-rec_Kir2.3
IPR013518 K_chnl_inward-rec_Kir_cyto
PANTHERiPTHR11767 PTHR11767, 1 hit
PTHR11767:SF53 PTHR11767:SF53, 1 hit
PfamiView protein in Pfam
PF01007 IRK, 1 hit
PIRSFiPIRSF005465 GIRK_kir, 1 hit
PRINTSiPR01326 KIR23CHANNEL
PR01320 KIRCHANNEL
SUPFAMiSSF81296 SSF81296, 1 hit

Sequencei

Sequence statusi: Complete.

P52189-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHGHNRNGQA HVPRRKRRNR FVKKNGQCNV YFANLSNKSQ RYMADIFTTC
60 70 80 90 100
VDTRWRYMLM IFSAAFLVSW LFFGLLFWWI AFFHGDLEAS PSVPAVGGPG
110 120 130 140 150
GNGGESPNAP KPCIMHVNGF LGAFLFSVET QTTIGYGFRC VTEECPLAVI
160 170 180 190 200
AVVVQSIVGC VIDSFMIGTI MAKMARPKKR AQTLLFSHHA VISVRDGKLC
210 220 230 240 250
LMWRVGNLRK SHIVEAHVRA QLIKPYMTQE GEYLPLDQRD LNVGYDIGLD
260 270 280 290 300
RIFLVSPIII VHEIDEDSPL YGMGKEELES EDFEIVVILE GMVEATAMTT
310 320 330 340 350
QARSSYLASE ILWGHRFEPV VFEEKSHYKV DYSRFHKTYE VAGTPCCSAR
360 370 380 390 400
ELQESKITVL PAPPPPPSAF CYENELALMS QEEEEMEEEA AAAAAVAAGL
410 420 430 440
GLEAGSKEEA GIIRMLEFGS HLDLERMQAA TLPLDNISYR RESRI
Length:445
Mass (Da):49,899
Last modified:October 1, 1996 - v1
Checksum:i09C9E889CC8E0DF3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti79W → C in AAA53241 (PubMed:7926018).Curated1
Sequence conflicti86D → E in AAA53241 (PubMed:7926018).Curated1
Sequence conflicti96V → A in AAA53241 (PubMed:7926018).Curated1
Sequence conflicti105E → A in AAA53241 (PubMed:7926018).Curated1
Sequence conflicti239R → G in AAA53241 (PubMed:7926018).Curated1
Sequence conflicti444R → A in AAA53241 (PubMed:7926018).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U11075 mRNA Translation: AAA53241.1
S71382 mRNA Translation: AAB31087.1
CCDSiCCDS27641.1
PIRiS45713
UniGeneiMm.140760
Mm.39759

Similar proteinsi

Entry informationi

Entry nameiKCNJ4_MOUSE
AccessioniPrimary (citable) accession number: P52189
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 23, 2018
This is version 133 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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