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P52188

- KCJ12_RAT

UniProt

P52188 - KCJ12_RAT

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Protein

ATP-sensitive inward rectifier potassium channel 12

Gene
Kcnj12, Irk2
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Inward rectifying potassium channel that is activated by phosphatidylinositol 4,5-bisphosphate and that probably participates in controlling the resting membrane potential in electrically excitable cells. Probably participates in establishing action potential waveform and excitability of neuronal and muscle tissues. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium.2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei173 – 1731Role in the control of polyamine-mediated channel gating and in the blocking by intracellular magnesium By similarity

GO - Molecular functioni

  1. inward rectifier potassium channel activity Source: UniProtKB
  2. PDZ domain binding Source: RGD
  3. protein binding Source: RGD

GO - Biological processi

  1. potassium ion transmembrane transport Source: GOC
  2. potassium ion transport Source: UniProtKB
  3. protein homotetramerization Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Potassium transport, Transport

Keywords - Ligandi

Potassium

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-sensitive inward rectifier potassium channel 12
Alternative name(s):
Inward rectifier K(+) channel Kir2.2
Short name:
IRK-2
Potassium channel, inwardly rectifying subfamily J member 12
Gene namesi
Name:Kcnj12
Synonyms:Irk2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi621660. Kcnj12.

Subcellular locationi

Membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 8282Cytoplasmic By similarityAdd
BLAST
Transmembranei83 – 10725Helical; Name=M1; By similarityAdd
BLAST
Topological domaini108 – 12316Extracellular By similarityAdd
BLAST
Intramembranei124 – 1285 By similarity
Intramembranei129 – 14113Helical; Pore-forming; Name=H5; By similarityAdd
BLAST
Intramembranei142 – 15110Pore-forming; By similarity
Topological domaini152 – 1565Extracellular By similarity
Transmembranei157 – 18125Helical; Name=M2; By similarityAdd
BLAST
Topological domaini182 – 427246Cytoplasmic By similarityAdd
BLAST

GO - Cellular componenti

  1. dendrite Source: RGD
  2. integral component of membrane Source: UniProtKB-KW
  3. intrinsic component of membrane Source: UniProtKB
  4. neuronal cell body Source: RGD
  5. T-tubule Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 427427ATP-sensitive inward rectifier potassium channel 12PRO_0000154964Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi155 – 155Interchain By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP52188.
PRIDEiP52188.

PTM databases

PhosphoSiteiP52188.

Expressioni

Tissue specificityi

Highest level in cerebellum. Moderately found in kidney, forebrain and skeletal muscle. Not detected in uterus, liver and pancreas.1 Publication

Gene expression databases

GenevestigatoriP52188.

Interactioni

Subunit structurei

Homotetramer. Forms heteromer with KCNJ4. Association, via its PDZ-recognition domain, with LIN7A, LIN7B, LIN7C, DLG1, CASK and APBA1 plays a key role in its localization and trafficking.

Protein-protein interaction databases

IntActiP52188. 8 interactions.
MINTiMINT-158957.
STRINGi10116.ENSRNOP00000003138.

Structurei

3D structure databases

ProteinModelPortaliP52188.
SMRiP52188. Positions 188-369.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi79 – 813Interaction with phosphatidylinositides By similarity
Motifi143 – 1486Selectivity filter By similarity
Motifi183 – 1908Interaction with phosphatidylinositides By similarity
Motifi425 – 4273PDZ-binding Reviewed prediction

Domaini

Phosphatidylinositol 4,5-bisphosphate binding to the cytoplasmic side of the channel triggers a conformation change leading to channel opening By similarity.

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG72812.
HOGENOMiHOG000237325.
HOVERGENiHBG006178.
InParanoidiP52188.
PhylomeDBiP52188.

Family and domain databases

Gene3Di2.60.40.1400. 1 hit.
InterProiIPR014756. Ig_E-set.
IPR016449. K_chnl_inward-rec_Kir.
IPR003272. K_chnl_inward-rec_Kir2.2.
IPR013518. K_chnl_inward-rec_Kir_cyto.
IPR013673. K_chnl_inward-rec_Kir_N.
[Graphical view]
PANTHERiPTHR11767. PTHR11767. 1 hit.
PTHR11767:SF14. PTHR11767:SF14. 1 hit.
PfamiPF01007. IRK. 1 hit.
PF08466. IRK_N. 1 hit.
[Graphical view]
PIRSFiPIRSF005465. GIRK_kir. 1 hit.
PRINTSiPR01325. KIR22CHANNEL.
PR01320. KIRCHANNEL.
SUPFAMiSSF81296. SSF81296. 1 hit.

Sequencei

Sequence statusi: Complete.

P52188-1 [UniParc]FASTAAdd to Basket

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MTAASRANPY SIVSSEEDGL HLVTMSGANG FGNGKVHTRR RCRNRFVKKN    50
GQCNIEFANM DEKSQRYLAD MFTTCVDIRW RYMLLIFSLA FLASWLLFGI 100
IFWVIAVAHG DLEPAEGRGR TPCVLQVHGF MAAFLFSIET QTTIGYGLRC 150
VTEECPVAVF MVVAQSIVGC IIDSFMIGAI MAKMGRPKKR AQTLLFSHNA 200
VVALRDGKLC LMWRVGNLRK SHIVEAHVRA QLIKPRVTEE GEYIPLDQID 250
IDVGFDKGLD RIFLVSPITI LHEIDEASPL FGISRQDLET DDFEIVVILE 300
GMVEATAMTT QARSSYLANE ILWGHRFEPV LFEEKNQYKI DYSHFHKTYE 350
VPSTPRCSAK DLVENKFLLP SANSFCYENE LAFLSRDEED EVATDRDGRS 400
PQPEHDFDRL QASSGALERP YRRESEI 427
Length:427
Mass (Da):48,399
Last modified:October 1, 1996 - v1
Checksum:i6FA100FEB6066677
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X78461 mRNA. Translation: CAA55216.1.
PIRiS43667.
UniGeneiRn.10406.

Genome annotation databases

UCSCiRGD:621660. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X78461 mRNA. Translation: CAA55216.1 .
PIRi S43667.
UniGenei Rn.10406.

3D structure databases

ProteinModelPortali P52188.
SMRi P52188. Positions 188-369.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P52188. 8 interactions.
MINTi MINT-158957.
STRINGi 10116.ENSRNOP00000003138.

Chemistry

GuidetoPHARMACOLOGYi 431.

PTM databases

PhosphoSitei P52188.

Proteomic databases

PaxDbi P52188.
PRIDEi P52188.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

UCSCi RGD:621660. rat.

Organism-specific databases

RGDi 621660. Kcnj12.

Phylogenomic databases

eggNOGi NOG72812.
HOGENOMi HOG000237325.
HOVERGENi HBG006178.
InParanoidi P52188.
PhylomeDBi P52188.

Miscellaneous databases

PROi P52188.

Gene expression databases

Genevestigatori P52188.

Family and domain databases

Gene3Di 2.60.40.1400. 1 hit.
InterProi IPR014756. Ig_E-set.
IPR016449. K_chnl_inward-rec_Kir.
IPR003272. K_chnl_inward-rec_Kir2.2.
IPR013518. K_chnl_inward-rec_Kir_cyto.
IPR013673. K_chnl_inward-rec_Kir_N.
[Graphical view ]
PANTHERi PTHR11767. PTHR11767. 1 hit.
PTHR11767:SF14. PTHR11767:SF14. 1 hit.
Pfami PF01007. IRK. 1 hit.
PF08466. IRK_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF005465. GIRK_kir. 1 hit.
PRINTSi PR01325. KIR22CHANNEL.
PR01320. KIRCHANNEL.
SUPFAMi SSF81296. SSF81296. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning, functional expression and localization of a novel inward rectifier potassium channel in the rat brain."
    Koyama H., Morishige K., Takahashi N., Zanelli J.S., Fass D.N., Kurachi Y.
    FEBS Lett. 341:303-307(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Tissue: Brain.
  2. "A multiprotein trafficking complex composed of SAP97, CASK, Veli, and Mint1 is associated with inward rectifier Kir2 potassium channels."
    Leonoudakis D., Conti L.R., Radeke C.M., McGuire L.M., Vandenberg C.A.
    J. Biol. Chem. 279:19051-19063(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CASK; LIN7A; LIN7B; LIN7C; APBA1 AND DLG1, FUNCTION.

Entry informationi

Entry nameiKCJ12_RAT
AccessioniPrimary (citable) accession number: P52188
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 11, 2014
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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