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P52188

- KCJ12_RAT

UniProt

P52188 - KCJ12_RAT

Protein

ATP-sensitive inward rectifier potassium channel 12

Gene

Kcnj12

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 113 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Inward rectifying potassium channel that is activated by phosphatidylinositol 4,5-bisphosphate and that probably participates in controlling the resting membrane potential in electrically excitable cells. Probably participates in establishing action potential waveform and excitability of neuronal and muscle tissues. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium.2 Publications

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei173 – 1731Role in the control of polyamine-mediated channel gating and in the blocking by intracellular magnesiumBy similarity

    GO - Molecular functioni

    1. inward rectifier potassium channel activity Source: UniProtKB
    2. PDZ domain binding Source: RGD
    3. protein binding Source: RGD

    GO - Biological processi

    1. potassium ion transmembrane transport Source: GOC
    2. potassium ion transport Source: UniProtKB
    3. protein homotetramerization Source: UniProtKB

    Keywords - Molecular functioni

    Ion channel, Voltage-gated channel

    Keywords - Biological processi

    Ion transport, Potassium transport, Transport

    Keywords - Ligandi

    Potassium

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ATP-sensitive inward rectifier potassium channel 12
    Alternative name(s):
    Inward rectifier K(+) channel Kir2.2
    Short name:
    IRK-2
    Potassium channel, inwardly rectifying subfamily J member 12
    Gene namesi
    Name:Kcnj12
    Synonyms:Irk2
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi621660. Kcnj12.

    Subcellular locationi

    Membrane 1 Publication; Multi-pass membrane protein 1 Publication. Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication

    GO - Cellular componenti

    1. dendrite Source: RGD
    2. integral component of membrane Source: UniProtKB-KW
    3. intrinsic component of membrane Source: UniProtKB
    4. neuronal cell body Source: RGD
    5. T-tubule Source: RGD

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 427427ATP-sensitive inward rectifier potassium channel 12PRO_0000154964Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi155 – 155InterchainBy similarity

    Keywords - PTMi

    Disulfide bond

    Proteomic databases

    PaxDbiP52188.
    PRIDEiP52188.

    PTM databases

    PhosphoSiteiP52188.

    Expressioni

    Tissue specificityi

    Highest level in cerebellum. Moderately found in kidney, forebrain and skeletal muscle. Not detected in uterus, liver and pancreas.1 Publication

    Gene expression databases

    GenevestigatoriP52188.

    Interactioni

    Subunit structurei

    Homotetramer. Forms heteromer with KCNJ4. Association, via its PDZ-recognition domain, with LIN7A, LIN7B, LIN7C, DLG1, CASK and APBA1 plays a key role in its localization and trafficking.

    Protein-protein interaction databases

    IntActiP52188. 8 interactions.
    MINTiMINT-158957.
    STRINGi10116.ENSRNOP00000003138.

    Structurei

    3D structure databases

    ProteinModelPortaliP52188.
    SMRiP52188. Positions 188-369.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 8282CytoplasmicBy similarityAdd
    BLAST
    Topological domaini108 – 12316ExtracellularBy similarityAdd
    BLAST
    Topological domaini152 – 1565ExtracellularBy similarity
    Topological domaini182 – 427246CytoplasmicBy similarityAdd
    BLAST

    Intramembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Intramembranei124 – 1285By similarity
    Intramembranei129 – 14113Helical; Pore-forming; Name=H5By similarityAdd
    BLAST
    Intramembranei142 – 15110Pore-formingBy similarity

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei83 – 10725Helical; Name=M1By similarityAdd
    BLAST
    Transmembranei157 – 18125Helical; Name=M2By similarityAdd
    BLAST

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi79 – 813Interaction with phosphatidylinositidesBy similarity
    Motifi143 – 1486Selectivity filterBy similarity
    Motifi183 – 1908Interaction with phosphatidylinositidesBy similarity
    Motifi425 – 4273PDZ-bindingSequence Analysis

    Domaini

    Phosphatidylinositol 4,5-bisphosphate binding to the cytoplasmic side of the channel triggers a conformation change leading to channel opening.By similarity

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG72812.
    HOGENOMiHOG000237325.
    HOVERGENiHBG006178.
    InParanoidiP52188.
    PhylomeDBiP52188.

    Family and domain databases

    Gene3Di2.60.40.1400. 1 hit.
    InterProiIPR014756. Ig_E-set.
    IPR016449. K_chnl_inward-rec_Kir.
    IPR003272. K_chnl_inward-rec_Kir2.2.
    IPR013518. K_chnl_inward-rec_Kir_cyto.
    IPR013673. K_chnl_inward-rec_Kir_N.
    [Graphical view]
    PANTHERiPTHR11767. PTHR11767. 1 hit.
    PTHR11767:SF14. PTHR11767:SF14. 1 hit.
    PfamiPF01007. IRK. 1 hit.
    PF08466. IRK_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005465. GIRK_kir. 1 hit.
    PRINTSiPR01325. KIR22CHANNEL.
    PR01320. KIRCHANNEL.
    SUPFAMiSSF81296. SSF81296. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P52188-1 [UniParc]FASTAAdd to Basket

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    MTAASRANPY SIVSSEEDGL HLVTMSGANG FGNGKVHTRR RCRNRFVKKN    50
    GQCNIEFANM DEKSQRYLAD MFTTCVDIRW RYMLLIFSLA FLASWLLFGI 100
    IFWVIAVAHG DLEPAEGRGR TPCVLQVHGF MAAFLFSIET QTTIGYGLRC 150
    VTEECPVAVF MVVAQSIVGC IIDSFMIGAI MAKMGRPKKR AQTLLFSHNA 200
    VVALRDGKLC LMWRVGNLRK SHIVEAHVRA QLIKPRVTEE GEYIPLDQID 250
    IDVGFDKGLD RIFLVSPITI LHEIDEASPL FGISRQDLET DDFEIVVILE 300
    GMVEATAMTT QARSSYLANE ILWGHRFEPV LFEEKNQYKI DYSHFHKTYE 350
    VPSTPRCSAK DLVENKFLLP SANSFCYENE LAFLSRDEED EVATDRDGRS 400
    PQPEHDFDRL QASSGALERP YRRESEI 427
    Length:427
    Mass (Da):48,399
    Last modified:October 1, 1996 - v1
    Checksum:i6FA100FEB6066677
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X78461 mRNA. Translation: CAA55216.1.
    PIRiS43667.
    UniGeneiRn.10406.

    Genome annotation databases

    UCSCiRGD:621660. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X78461 mRNA. Translation: CAA55216.1 .
    PIRi S43667.
    UniGenei Rn.10406.

    3D structure databases

    ProteinModelPortali P52188.
    SMRi P52188. Positions 188-369.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P52188. 8 interactions.
    MINTi MINT-158957.
    STRINGi 10116.ENSRNOP00000003138.

    Chemistry

    GuidetoPHARMACOLOGYi 431.

    PTM databases

    PhosphoSitei P52188.

    Proteomic databases

    PaxDbi P52188.
    PRIDEi P52188.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    UCSCi RGD:621660. rat.

    Organism-specific databases

    RGDi 621660. Kcnj12.

    Phylogenomic databases

    eggNOGi NOG72812.
    HOGENOMi HOG000237325.
    HOVERGENi HBG006178.
    InParanoidi P52188.
    PhylomeDBi P52188.

    Miscellaneous databases

    PROi P52188.

    Gene expression databases

    Genevestigatori P52188.

    Family and domain databases

    Gene3Di 2.60.40.1400. 1 hit.
    InterProi IPR014756. Ig_E-set.
    IPR016449. K_chnl_inward-rec_Kir.
    IPR003272. K_chnl_inward-rec_Kir2.2.
    IPR013518. K_chnl_inward-rec_Kir_cyto.
    IPR013673. K_chnl_inward-rec_Kir_N.
    [Graphical view ]
    PANTHERi PTHR11767. PTHR11767. 1 hit.
    PTHR11767:SF14. PTHR11767:SF14. 1 hit.
    Pfami PF01007. IRK. 1 hit.
    PF08466. IRK_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005465. GIRK_kir. 1 hit.
    PRINTSi PR01325. KIR22CHANNEL.
    PR01320. KIRCHANNEL.
    SUPFAMi SSF81296. SSF81296. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning, functional expression and localization of a novel inward rectifier potassium channel in the rat brain."
      Koyama H., Morishige K., Takahashi N., Zanelli J.S., Fass D.N., Kurachi Y.
      FEBS Lett. 341:303-307(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
      Tissue: Brain.
    2. "A multiprotein trafficking complex composed of SAP97, CASK, Veli, and Mint1 is associated with inward rectifier Kir2 potassium channels."
      Leonoudakis D., Conti L.R., Radeke C.M., McGuire L.M., Vandenberg C.A.
      J. Biol. Chem. 279:19051-19063(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CASK; LIN7A; LIN7B; LIN7C; APBA1 AND DLG1, FUNCTION.

    Entry informationi

    Entry nameiKCJ12_RAT
    AccessioniPrimary (citable) accession number: P52188
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 113 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3