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P52188 (IRK12_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 100. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
ATP-sensitive inward rectifier potassium channel 12
Alternative name(s):
Inward rectifier K(+) channel Kir2.2
Short name=IRK-2
Potassium channel, inwardly rectifying subfamily J member 12
Gene names
Name:Kcnj12
Synonyms:Irk2
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length427 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Probably participates in establishing action potential waveform and excitability of neuronal and muscle tissues. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium and cesium. Ref.2

Subunit structure

Association, via its PDZ-recognition domain, with LIN7A, LIN7B, LIN7C, DLG1, CASK and APBA1 plays a key role in its localization and trafficking.

Subcellular location

Membrane; Multi-pass membrane protein.

Tissue specificity

Highest level in cerebellum. Moderately found in kidney, forebrain and skeletal muscle. Not detected in uterus, liver and pancreas.

Sequence similarities

Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ12 subfamily. [View classification]

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 427427ATP-sensitive inward rectifier potassium channel 12
PRO_0000154964

Regions

Topological domain1 – 8080Cytoplasmic By similarity
Transmembrane81 – 10525Helical; Name=M1; By similarity
Topological domain106 – 12924Extracellular By similarity
Intramembrane130 – 14112Helical; Pore-forming; Name=H5; By similarity
Intramembrane142 – 1487Pore-forming; By similarity
Topological domain149 – 1579Extracellular By similarity
Transmembrane158 – 17922Helical; Name=M2; By similarity
Topological domain180 – 427248Cytoplasmic By similarity
Motif143 – 1486Selectivity filter By similarity
Motif425 – 4273PDZ-binding Potential

Sites

Site1731Role in the control of polyamine-mediated channel gating and in the blocking by intracellular magnesium By similarity

Sequences

Sequence LengthMass (Da)Tools
P52188 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 6FA100FEB6066677

FASTA42748,399
        10         20         30         40         50         60 
MTAASRANPY SIVSSEEDGL HLVTMSGANG FGNGKVHTRR RCRNRFVKKN GQCNIEFANM 

        70         80         90        100        110        120 
DEKSQRYLAD MFTTCVDIRW RYMLLIFSLA FLASWLLFGI IFWVIAVAHG DLEPAEGRGR 

       130        140        150        160        170        180 
TPCVLQVHGF MAAFLFSIET QTTIGYGLRC VTEECPVAVF MVVAQSIVGC IIDSFMIGAI 

       190        200        210        220        230        240 
MAKMGRPKKR AQTLLFSHNA VVALRDGKLC LMWRVGNLRK SHIVEAHVRA QLIKPRVTEE 

       250        260        270        280        290        300 
GEYIPLDQID IDVGFDKGLD RIFLVSPITI LHEIDEASPL FGISRQDLET DDFEIVVILE 

       310        320        330        340        350        360 
GMVEATAMTT QARSSYLANE ILWGHRFEPV LFEEKNQYKI DYSHFHKTYE VPSTPRCSAK 

       370        380        390        400        410        420 
DLVENKFLLP SANSFCYENE LAFLSRDEED EVATDRDGRS PQPEHDFDRL QASSGALERP 


YRRESEI

« Hide

References

[1]"Molecular cloning, functional expression and localization of a novel inward rectifier potassium channel in the rat brain."
Koyama H., Morishige K., Takahashi N., Zanelli J.S., Fass D.N., Kurachi Y.
FEBS Lett. 341:303-307(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
[2]"A multiprotein trafficking complex composed of SAP97, CASK, Veli, and Mint1 is associated with inward rectifier Kir2 potassium channels."
Leonoudakis D., Conti L.R., Radeke C.M., McGuire L.M., Vandenberg C.A.
J. Biol. Chem. 279:19051-19063(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH CASK; LIN7A; LIN7B; LIN7C; APBA1 AND DLG1, FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X78461 mRNA. Translation: CAA55216.1.
IPIIPI00777371.
PIRS43667.
UniGeneRn.10406.

3D structure databases

ProteinModelPortalP52188.
SMRP52188. Positions 188-369.
ModBaseSearch...

Protein-protein interaction databases

IntActP52188. 8 interactions.
MINTMINT-158957.
STRING10116.ENSRNOP00000003138.

PTM databases

PhosphoSiteP52188.

Proteomic databases

PaxDbP52188.
PRIDEP52188.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

UCSCRGD:621660. rat.

Organism-specific databases

RGD621660. Kcnj12.

Phylogenomic databases

eggNOGNOG72812.
HOGENOMHOG000237325.
HOVERGENHBG006178.
InParanoidP52188.
OrthoDBEOG4N04DV.

Gene expression databases

ArrayExpressP52188.
GenevestigatorP52188.
GermOnlineENSRNOG00000002303. Rattus norvegicus.

Family and domain databases

Gene3D2.60.40.1400. 1 hit.
InterProIPR014756. Ig_E-set.
IPR016449. K_chnl_inward-rec_Kir.
IPR003272. K_chnl_inward-rec_Kir2.2.
IPR013518. K_chnl_inward-rec_Kir_cyto.
IPR013673. K_chnl_inward-rec_Kir_N.
[Graphical view]
PANTHERPTHR11767. PTHR11767. 1 hit.
PTHR11767:SF14. PTHR11767:SF14. 1 hit.
PfamPF01007. IRK. 1 hit.
PF08466. IRK_N. 1 hit.
[Graphical view]
PIRSFPIRSF005465. GIRK_kir. 1 hit.
PRINTSPR01325. KIR22CHANNEL.
PR01320. KIRCHANNEL.
SUPFAMSSF81296. Ig_E-set. 1 hit.
ProtoNetSearch...

Entry information

Entry nameIRK12_RAT
AccessionPrimary (citable) accession number: P52188
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 3, 2013
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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