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P52187

- KCJ12_MOUSE

UniProt

P52187 - KCJ12_MOUSE

Protein

ATP-sensitive inward rectifier potassium channel 12

Gene

Kcnj12

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 2 (03 Oct 2012)
      Previous versions | rss
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    Functioni

    Inward rectifying potassium channel that is activated by phosphatidylinositol 4,5-bisphosphate and that probably participates in controlling the resting membrane potential in electrically excitable cells. Probably participates in establishing action potential waveform and excitability of neuronal and muscle tissues. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei173 – 1731Role in the control of polyamine-mediated channel gating and in the blocking by intracellular magnesiumBy similarity

    GO - Molecular functioni

    1. inward rectifier potassium channel activity Source: UniProtKB

    GO - Biological processi

    1. potassium ion transmembrane transport Source: GOC
    2. potassium ion transport Source: UniProtKB
    3. protein homotetramerization Source: UniProtKB

    Keywords - Molecular functioni

    Ion channel, Voltage-gated channel

    Keywords - Biological processi

    Ion transport, Potassium transport, Transport

    Keywords - Ligandi

    Potassium

    Enzyme and pathway databases

    ReactomeiREACT_199076. Classical Kir channels.
    REACT_208837. Activation of G protein gated Potassium channels.
    REACT_226457. Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ATP-sensitive inward rectifier potassium channel 12
    Alternative name(s):
    Inward rectifier K(+) channel Kir2.2
    Short name:
    IRK-2
    Potassium channel, inwardly rectifying subfamily J member 12
    Gene namesi
    Name:Kcnj12
    Synonyms:Irk2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 11

    Organism-specific databases

    MGIiMGI:108495. Kcnj12.

    Subcellular locationi

    Membrane 1 Publication; Multi-pass membrane protein 1 Publication. Cell membrane By similarity; Multi-pass membrane protein By similarity

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. intrinsic component of membrane Source: UniProtKB
    3. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 427427ATP-sensitive inward rectifier potassium channel 12PRO_0000154963Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi155 – 155InterchainBy similarity

    Keywords - PTMi

    Disulfide bond

    Proteomic databases

    PRIDEiP52187.

    Expressioni

    Tissue specificityi

    Highest level in cerebellum.1 Publication

    Gene expression databases

    GenevestigatoriP52187.

    Interactioni

    Subunit structurei

    Homotetramer. Forms heteromer with KCNJ4. Association, via its PDZ-recognition domain, with LIN7A, LIN7B, LIN7C, DLG1, CASK and APBA1 plays a key role in its localization and trafficking By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP52187.
    SMRiP52187. Positions 42-372.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 8282CytoplasmicBy similarityAdd
    BLAST
    Topological domaini108 – 12316ExtracellularBy similarityAdd
    BLAST
    Topological domaini152 – 1565ExtracellularBy similarity
    Topological domaini182 – 427246CytoplasmicBy similarityAdd
    BLAST

    Intramembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Intramembranei124 – 1285By similarity
    Intramembranei129 – 14113Helical; Pore-forming; Name=H5By similarityAdd
    BLAST
    Intramembranei142 – 15110Pore-formingBy similarity

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei83 – 10725Helical; Name=M1By similarityAdd
    BLAST
    Transmembranei157 – 18125Helical; Name=M2By similarityAdd
    BLAST

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi79 – 813Interaction with phosphatidylinositidesBy similarity
    Motifi143 – 1486Selectivity filterBy similarity
    Motifi183 – 1908Interaction with phosphatidylinositidesBy similarity
    Motifi425 – 4273PDZ-bindingSequence Analysis

    Domaini

    Phosphatidylinositol 4,5-bisphosphate binding to the cytoplasmic side of the channel triggers a conformation change leading to channel opening.By similarity

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG72812.
    GeneTreeiENSGT00650000093228.
    HOGENOMiHOG000237325.
    HOVERGENiHBG006178.
    InParanoidiP52187.
    KOiK05005.
    OrthoDBiEOG7XPZ5K.
    TreeFamiTF313676.

    Family and domain databases

    Gene3Di2.60.40.1400. 1 hit.
    InterProiIPR014756. Ig_E-set.
    IPR016449. K_chnl_inward-rec_Kir.
    IPR003272. K_chnl_inward-rec_Kir2.2.
    IPR013518. K_chnl_inward-rec_Kir_cyto.
    IPR013673. K_chnl_inward-rec_Kir_N.
    [Graphical view]
    PANTHERiPTHR11767. PTHR11767. 1 hit.
    PTHR11767:SF14. PTHR11767:SF14. 1 hit.
    PfamiPF01007. IRK. 1 hit.
    PF08466. IRK_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005465. GIRK_kir. 1 hit.
    PRINTSiPR01325. KIR22CHANNEL.
    PR01320. KIRCHANNEL.
    SUPFAMiSSF81296. SSF81296. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P52187-1 [UniParc]FASTAAdd to Basket

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    MTAASRANPY SIVSSEEDGL HLVTMSGANG FGNGKVHTRR RCRNRFVKKN    50
    GQCNIEFANM DEKSQRYLAD MFTTCVDIRW RYMLLIFSLA FLASWLLFGI 100
    IFWVIAVAHG DLEPAEGRGR TPCVLQVHGF MAAFLFSIET QTTIGYGLRC 150
    VTEECPVAVF MVVAQSIVGC IIDSFMIGAI MAKMARPKKR AQTLLFSHNA 200
    VVALRDGKLC LMWRVGNLRK SHIVEAHVRA QLIKPRVTEE GEYIPLDQID 250
    IDVGFDKGLD RIFLVSPITI LHEIDEASPL FGISRQDLET DDFEIVVILE 300
    GMVEATAMTT QARSSYLANE ILWGHRFEPV LFEEKNQYKI DYSHFHKTYE 350
    VPSTPRCSAK DLVENKFLLP SANSFCYENE LAFLSRDEED EVATDRDGRS 400
    PQPEHDFDRL QASSAALERP YRRESEI 427
    Length:427
    Mass (Da):48,427
    Last modified:October 3, 2012 - v2
    Checksum:i3FA101CCF8BCF6AC
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti177 – 1771I → N in CAA56622. (PubMed:8083233)Curated
    Sequence conflicti255 – 2551F → L in BAA88471. 1 PublicationCurated
    Sequence conflicti385 – 3851S → I in CAA56622. (PubMed:8083233)Curated
    Sequence conflicti393 – 3931A → S in CAA56622. (PubMed:8083233)Curated
    Sequence conflicti398 – 4003GRS → VRT in CAA56622. (PubMed:8083233)Curated
    Sequence conflicti418 – 4181E → V in CAA56622. (PubMed:8083233)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X80417 mRNA. Translation: CAA56622.1.
    AK032271 mRNA. Translation: BAC27788.1.
    AL646093 Genomic DNA. Translation: CAI25892.1.
    BC115970 mRNA. Translation: AAI15971.1.
    BC116677 mRNA. Translation: AAI16678.1.
    AB035889 mRNA. Translation: BAA88471.1.
    CCDSiCCDS24805.1.
    RefSeqiNP_001254522.1. NM_001267593.1.
    XP_006532381.1. XM_006532318.1.
    UniGeneiMm.4970.

    Genome annotation databases

    EnsembliENSMUST00000089184; ENSMUSP00000086588; ENSMUSG00000042529.
    ENSMUST00000108717; ENSMUSP00000104357; ENSMUSG00000042529.
    GeneIDi16515.
    KEGGimmu:16515.
    UCSCiuc007jgy.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X80417 mRNA. Translation: CAA56622.1 .
    AK032271 mRNA. Translation: BAC27788.1 .
    AL646093 Genomic DNA. Translation: CAI25892.1 .
    BC115970 mRNA. Translation: AAI15971.1 .
    BC116677 mRNA. Translation: AAI16678.1 .
    AB035889 mRNA. Translation: BAA88471.1 .
    CCDSi CCDS24805.1.
    RefSeqi NP_001254522.1. NM_001267593.1.
    XP_006532381.1. XM_006532318.1.
    UniGenei Mm.4970.

    3D structure databases

    ProteinModelPortali P52187.
    SMRi P52187. Positions 42-372.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    GuidetoPHARMACOLOGYi 431.

    Proteomic databases

    PRIDEi P52187.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000089184 ; ENSMUSP00000086588 ; ENSMUSG00000042529 .
    ENSMUST00000108717 ; ENSMUSP00000104357 ; ENSMUSG00000042529 .
    GeneIDi 16515.
    KEGGi mmu:16515.
    UCSCi uc007jgy.2. mouse.

    Organism-specific databases

    CTDi 3768.
    MGIi MGI:108495. Kcnj12.

    Phylogenomic databases

    eggNOGi NOG72812.
    GeneTreei ENSGT00650000093228.
    HOGENOMi HOG000237325.
    HOVERGENi HBG006178.
    InParanoidi P52187.
    KOi K05005.
    OrthoDBi EOG7XPZ5K.
    TreeFami TF313676.

    Enzyme and pathway databases

    Reactomei REACT_199076. Classical Kir channels.
    REACT_208837. Activation of G protein gated Potassium channels.
    REACT_226457. Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits.

    Miscellaneous databases

    NextBioi 289869.
    PROi P52187.
    SOURCEi Search...

    Gene expression databases

    Genevestigatori P52187.

    Family and domain databases

    Gene3Di 2.60.40.1400. 1 hit.
    InterProi IPR014756. Ig_E-set.
    IPR016449. K_chnl_inward-rec_Kir.
    IPR003272. K_chnl_inward-rec_Kir2.2.
    IPR013518. K_chnl_inward-rec_Kir_cyto.
    IPR013673. K_chnl_inward-rec_Kir_N.
    [Graphical view ]
    PANTHERi PTHR11767. PTHR11767. 1 hit.
    PTHR11767:SF14. PTHR11767:SF14. 1 hit.
    Pfami PF01007. IRK. 1 hit.
    PF08466. IRK_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005465. GIRK_kir. 1 hit.
    PRINTSi PR01325. KIR22CHANNEL.
    PR01320. KIRCHANNEL.
    SUPFAMi SSF81296. SSF81296. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and functional expression of cDNA encoding a second class of inward rectifier potassium channels in the mouse brain."
      Takahashi N., Morishige K., Jahangir A., Yamada M., Findlay I., Koyama H., Kurachi Y.
      J. Biol. Chem. 269:23274-23279(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
      Strain: BALB/c.
      Tissue: Brain.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Olfactory bulb.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    5. "Inward recifier potassium channel IRK2."
      Ikeda K., Kobayashi T.
      Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 10-420.
      Strain: C57BL/6NJcl.
      Tissue: Brain.

    Entry informationi

    Entry nameiKCJ12_MOUSE
    AccessioniPrimary (citable) accession number: P52187
    Secondary accession number(s): Q8CCR0, Q9QYF2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 3, 2012
    Last modified: October 1, 2014
    This is version 116 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3