Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

ATP-sensitive inward rectifier potassium channel 12

Gene

Kcnj12

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Inward rectifying potassium channel that is activated by phosphatidylinositol 4,5-bisphosphate and that probably participates in controlling the resting membrane potential in electrically excitable cells. Probably participates in establishing action potential waveform and excitability of neuronal and muscle tissues. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei173Role in the control of polyamine-mediated channel gating and in the blocking by intracellular magnesiumBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIon channel, Voltage-gated channel
Biological processIon transport, Potassium transport, Transport
LigandPotassium

Enzyme and pathway databases

ReactomeiR-MMU-1296041 Activation of G protein gated Potassium channels
R-MMU-1296053 Classical Kir channels
R-MMU-5576886 Phase 4 - resting membrane potential
R-MMU-997272 Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-sensitive inward rectifier potassium channel 12
Alternative name(s):
Inward rectifier K(+) channel Kir2.2
Short name:
IRK-2
Potassium channel, inwardly rectifying subfamily J member 12
Gene namesi
Name:Kcnj12
Synonyms:Irk2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:108495 Kcnj12

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 82CytoplasmicBy similarityAdd BLAST82
Transmembranei83 – 107Helical; Name=M1By similarityAdd BLAST25
Topological domaini108 – 123ExtracellularBy similarityAdd BLAST16
Intramembranei124 – 128By similarity5
Intramembranei129 – 141Helical; Pore-forming; Name=H5By similarityAdd BLAST13
Intramembranei142 – 151Pore-formingBy similarity10
Topological domaini152 – 156ExtracellularBy similarity5
Transmembranei157 – 181Helical; Name=M2By similarityAdd BLAST25
Topological domaini182 – 427CytoplasmicBy similarityAdd BLAST246

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Chemistry databases

GuidetoPHARMACOLOGYi431

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001549631 – 427ATP-sensitive inward rectifier potassium channel 12Add BLAST427

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei75S-nitrosocysteineBy similarity1
Disulfide bondi155InterchainBy similarity

Keywords - PTMi

Disulfide bond, S-nitrosylation

Proteomic databases

EPDiP52187
PaxDbiP52187
PRIDEiP52187

PTM databases

iPTMnetiP52187
PhosphoSitePlusiP52187

Expressioni

Tissue specificityi

Highest level in cerebellum.1 Publication

Gene expression databases

BgeeiENSMUSG00000042529
ExpressionAtlasiP52187 baseline and differential
GenevisibleiP52187 MM

Interactioni

Subunit structurei

Homotetramer. Forms heteromer with KCNJ4. Association, via its PDZ-recognition domain, with LIN7A, LIN7B, LIN7C, DLG1, CASK and APBA1 plays a key role in its localization and trafficking (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000086588

Structurei

3D structure databases

ProteinModelPortaliP52187
SMRiP52187
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi79 – 81Interaction with phosphatidylinositidesBy similarity3
Motifi143 – 148Selectivity filterBy similarity6
Motifi183 – 190Interaction with phosphatidylinositidesBy similarity8
Motifi425 – 427PDZ-bindingSequence analysis3

Domaini

Phosphatidylinositol 4,5-bisphosphate binding to the cytoplasmic side of the channel triggers a conformation change leading to channel opening.By similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3827 Eukaryota
ENOG410XQ62 LUCA
GeneTreeiENSGT00760000118842
HOGENOMiHOG000237325
HOVERGENiHBG006178
InParanoidiP52187
KOiK05005
TreeFamiTF313676

Family and domain databases

Gene3Di2.60.40.1400, 2 hits
InterProiView protein in InterPro
IPR014756 Ig_E-set
IPR016449 K_chnl_inward-rec_Kir
IPR003272 K_chnl_inward-rec_Kir2.2
IPR013518 K_chnl_inward-rec_Kir_cyto
IPR013673 K_chnl_inward-rec_Kir_N
PANTHERiPTHR11767 PTHR11767, 1 hit
PTHR11767:SF14 PTHR11767:SF14, 1 hit
PfamiView protein in Pfam
PF01007 IRK, 1 hit
PF08466 IRK_N, 1 hit
PIRSFiPIRSF005465 GIRK_kir, 1 hit
PRINTSiPR01325 KIR22CHANNEL
PR01320 KIRCHANNEL
SUPFAMiSSF81296 SSF81296, 1 hit

Sequencei

Sequence statusi: Complete.

P52187-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTAASRANPY SIVSSEEDGL HLVTMSGANG FGNGKVHTRR RCRNRFVKKN
60 70 80 90 100
GQCNIEFANM DEKSQRYLAD MFTTCVDIRW RYMLLIFSLA FLASWLLFGI
110 120 130 140 150
IFWVIAVAHG DLEPAEGRGR TPCVLQVHGF MAAFLFSIET QTTIGYGLRC
160 170 180 190 200
VTEECPVAVF MVVAQSIVGC IIDSFMIGAI MAKMARPKKR AQTLLFSHNA
210 220 230 240 250
VVALRDGKLC LMWRVGNLRK SHIVEAHVRA QLIKPRVTEE GEYIPLDQID
260 270 280 290 300
IDVGFDKGLD RIFLVSPITI LHEIDEASPL FGISRQDLET DDFEIVVILE
310 320 330 340 350
GMVEATAMTT QARSSYLANE ILWGHRFEPV LFEEKNQYKI DYSHFHKTYE
360 370 380 390 400
VPSTPRCSAK DLVENKFLLP SANSFCYENE LAFLSRDEED EVATDRDGRS
410 420
PQPEHDFDRL QASSAALERP YRRESEI
Length:427
Mass (Da):48,427
Last modified:October 3, 2012 - v2
Checksum:i3FA101CCF8BCF6AC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti177I → N in CAA56622 (PubMed:8083233).Curated1
Sequence conflicti255F → L in BAA88471 (Ref. 5) Curated1
Sequence conflicti385S → I in CAA56622 (PubMed:8083233).Curated1
Sequence conflicti393A → S in CAA56622 (PubMed:8083233).Curated1
Sequence conflicti398 – 400GRS → VRT in CAA56622 (PubMed:8083233).Curated3
Sequence conflicti418E → V in CAA56622 (PubMed:8083233).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X80417 mRNA Translation: CAA56622.1
AK032271 mRNA Translation: BAC27788.1
AL646093 Genomic DNA No translation available.
BC115970 mRNA Translation: AAI15971.1
BC116677 mRNA Translation: AAI16678.1
AB035889 mRNA Translation: BAA88471.1
CCDSiCCDS24805.1
RefSeqiNP_001254522.1, NM_001267593.1
XP_006532381.1, XM_006532318.3
UniGeneiMm.4970

Genome annotation databases

EnsembliENSMUST00000089184; ENSMUSP00000086588; ENSMUSG00000042529
ENSMUST00000108717; ENSMUSP00000104357; ENSMUSG00000042529
GeneIDi16515
KEGGimmu:16515
UCSCiuc007jgy.3 mouse

Similar proteinsi

Entry informationi

Entry nameiKCJ12_MOUSE
AccessioniPrimary (citable) accession number: P52187
Secondary accession number(s): Q8CCR0, Q9QYF2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 3, 2012
Last modified: May 23, 2018
This is version 141 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health