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Protein

ATP-sensitive inward rectifier potassium channel 12

Gene

Kcnj12

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Inward rectifying potassium channel that is activated by phosphatidylinositol 4,5-bisphosphate and that probably participates in controlling the resting membrane potential in electrically excitable cells. Probably participates in establishing action potential waveform and excitability of neuronal and muscle tissues. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei173 – 1731Role in the control of polyamine-mediated channel gating and in the blocking by intracellular magnesiumBy similarity

GO - Molecular functioni

  1. inward rectifier potassium channel activity Source: UniProtKB

GO - Biological processi

  1. potassium ion import Source: GO_Central
  2. potassium ion transport Source: UniProtKB
  3. protein homotetramerization Source: UniProtKB
  4. regulation of ion transmembrane transport Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Potassium transport, Transport

Keywords - Ligandi

Potassium

Enzyme and pathway databases

ReactomeiREACT_199076. Classical Kir channels.
REACT_208837. Activation of G protein gated Potassium channels.
REACT_226457. Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-sensitive inward rectifier potassium channel 12
Alternative name(s):
Inward rectifier K(+) channel Kir2.2
Short name:
IRK-2
Potassium channel, inwardly rectifying subfamily J member 12
Gene namesi
Name:Kcnj12
Synonyms:Irk2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 11

Organism-specific databases

MGIiMGI:108495. Kcnj12.

Subcellular locationi

Membrane 1 Publication; Multi-pass membrane protein 1 Publication. Cell membrane By similarity; Multi-pass membrane protein By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 8282CytoplasmicBy similarityAdd
BLAST
Transmembranei83 – 10725Helical; Name=M1By similarityAdd
BLAST
Topological domaini108 – 12316ExtracellularBy similarityAdd
BLAST
Intramembranei124 – 1285By similarity
Intramembranei129 – 14113Helical; Pore-forming; Name=H5By similarityAdd
BLAST
Intramembranei142 – 15110Pore-formingBy similarity
Topological domaini152 – 1565ExtracellularBy similarity
Transmembranei157 – 18125Helical; Name=M2By similarityAdd
BLAST
Topological domaini182 – 427246CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

  1. integral component of plasma membrane Source: GO_Central
  2. intrinsic component of membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 427427ATP-sensitive inward rectifier potassium channel 12PRO_0000154963Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei75 – 751S-nitrosocysteineBy similarity
Disulfide bondi155 – 155InterchainBy similarity

Keywords - PTMi

Disulfide bond, S-nitrosylation

Proteomic databases

PRIDEiP52187.

Expressioni

Tissue specificityi

Highest level in cerebellum.1 Publication

Gene expression databases

ExpressionAtlasiP52187. baseline and differential.
GenevestigatoriP52187.

Interactioni

Subunit structurei

Homotetramer. Forms heteromer with KCNJ4. Association, via its PDZ-recognition domain, with LIN7A, LIN7B, LIN7C, DLG1, CASK and APBA1 plays a key role in its localization and trafficking (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliP52187.
SMRiP52187. Positions 42-372.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi79 – 813Interaction with phosphatidylinositidesBy similarity
Motifi143 – 1486Selectivity filterBy similarity
Motifi183 – 1908Interaction with phosphatidylinositidesBy similarity
Motifi425 – 4273PDZ-bindingSequence Analysis

Domaini

Phosphatidylinositol 4,5-bisphosphate binding to the cytoplasmic side of the channel triggers a conformation change leading to channel opening.By similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG72812.
GeneTreeiENSGT00760000118842.
HOGENOMiHOG000237325.
HOVERGENiHBG006178.
InParanoidiP52187.
KOiK05005.
OrthoDBiEOG7XPZ5K.
TreeFamiTF313676.

Family and domain databases

Gene3Di2.60.40.1400. 1 hit.
InterProiIPR014756. Ig_E-set.
IPR016449. K_chnl_inward-rec_Kir.
IPR003272. K_chnl_inward-rec_Kir2.2.
IPR013518. K_chnl_inward-rec_Kir_cyto.
IPR013673. K_chnl_inward-rec_Kir_N.
[Graphical view]
PANTHERiPTHR11767. PTHR11767. 1 hit.
PTHR11767:SF14. PTHR11767:SF14. 1 hit.
PfamiPF01007. IRK. 1 hit.
PF08466. IRK_N. 1 hit.
[Graphical view]
PIRSFiPIRSF005465. GIRK_kir. 1 hit.
PRINTSiPR01325. KIR22CHANNEL.
PR01320. KIRCHANNEL.
SUPFAMiSSF81296. SSF81296. 1 hit.

Sequencei

Sequence statusi: Complete.

P52187-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTAASRANPY SIVSSEEDGL HLVTMSGANG FGNGKVHTRR RCRNRFVKKN
60 70 80 90 100
GQCNIEFANM DEKSQRYLAD MFTTCVDIRW RYMLLIFSLA FLASWLLFGI
110 120 130 140 150
IFWVIAVAHG DLEPAEGRGR TPCVLQVHGF MAAFLFSIET QTTIGYGLRC
160 170 180 190 200
VTEECPVAVF MVVAQSIVGC IIDSFMIGAI MAKMARPKKR AQTLLFSHNA
210 220 230 240 250
VVALRDGKLC LMWRVGNLRK SHIVEAHVRA QLIKPRVTEE GEYIPLDQID
260 270 280 290 300
IDVGFDKGLD RIFLVSPITI LHEIDEASPL FGISRQDLET DDFEIVVILE
310 320 330 340 350
GMVEATAMTT QARSSYLANE ILWGHRFEPV LFEEKNQYKI DYSHFHKTYE
360 370 380 390 400
VPSTPRCSAK DLVENKFLLP SANSFCYENE LAFLSRDEED EVATDRDGRS
410 420
PQPEHDFDRL QASSAALERP YRRESEI
Length:427
Mass (Da):48,427
Last modified:October 3, 2012 - v2
Checksum:i3FA101CCF8BCF6AC
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti177 – 1771I → N in CAA56622 (PubMed:8083233).Curated
Sequence conflicti255 – 2551F → L in BAA88471 (Ref. 5) Curated
Sequence conflicti385 – 3851S → I in CAA56622 (PubMed:8083233).Curated
Sequence conflicti393 – 3931A → S in CAA56622 (PubMed:8083233).Curated
Sequence conflicti398 – 4003GRS → VRT in CAA56622 (PubMed:8083233).Curated
Sequence conflicti418 – 4181E → V in CAA56622 (PubMed:8083233).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X80417 mRNA. Translation: CAA56622.1.
AK032271 mRNA. Translation: BAC27788.1.
AL646093 Genomic DNA. Translation: CAI25892.1.
BC115970 mRNA. Translation: AAI15971.1.
BC116677 mRNA. Translation: AAI16678.1.
AB035889 mRNA. Translation: BAA88471.1.
CCDSiCCDS24805.1.
RefSeqiNP_001254522.1. NM_001267593.1.
XP_006532381.1. XM_006532318.1.
UniGeneiMm.4970.

Genome annotation databases

EnsembliENSMUST00000089184; ENSMUSP00000086588; ENSMUSG00000042529.
ENSMUST00000108717; ENSMUSP00000104357; ENSMUSG00000042529.
GeneIDi16515.
KEGGimmu:16515.
UCSCiuc007jgy.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X80417 mRNA. Translation: CAA56622.1.
AK032271 mRNA. Translation: BAC27788.1.
AL646093 Genomic DNA. Translation: CAI25892.1.
BC115970 mRNA. Translation: AAI15971.1.
BC116677 mRNA. Translation: AAI16678.1.
AB035889 mRNA. Translation: BAA88471.1.
CCDSiCCDS24805.1.
RefSeqiNP_001254522.1. NM_001267593.1.
XP_006532381.1. XM_006532318.1.
UniGeneiMm.4970.

3D structure databases

ProteinModelPortaliP52187.
SMRiP52187. Positions 42-372.
ModBaseiSearch...
MobiDBiSearch...

Chemistry

GuidetoPHARMACOLOGYi431.

Proteomic databases

PRIDEiP52187.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000089184; ENSMUSP00000086588; ENSMUSG00000042529.
ENSMUST00000108717; ENSMUSP00000104357; ENSMUSG00000042529.
GeneIDi16515.
KEGGimmu:16515.
UCSCiuc007jgy.2. mouse.

Organism-specific databases

CTDi3768.
MGIiMGI:108495. Kcnj12.

Phylogenomic databases

eggNOGiNOG72812.
GeneTreeiENSGT00760000118842.
HOGENOMiHOG000237325.
HOVERGENiHBG006178.
InParanoidiP52187.
KOiK05005.
OrthoDBiEOG7XPZ5K.
TreeFamiTF313676.

Enzyme and pathway databases

ReactomeiREACT_199076. Classical Kir channels.
REACT_208837. Activation of G protein gated Potassium channels.
REACT_226457. Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits.

Miscellaneous databases

NextBioi289869.
PROiP52187.
SOURCEiSearch...

Gene expression databases

ExpressionAtlasiP52187. baseline and differential.
GenevestigatoriP52187.

Family and domain databases

Gene3Di2.60.40.1400. 1 hit.
InterProiIPR014756. Ig_E-set.
IPR016449. K_chnl_inward-rec_Kir.
IPR003272. K_chnl_inward-rec_Kir2.2.
IPR013518. K_chnl_inward-rec_Kir_cyto.
IPR013673. K_chnl_inward-rec_Kir_N.
[Graphical view]
PANTHERiPTHR11767. PTHR11767. 1 hit.
PTHR11767:SF14. PTHR11767:SF14. 1 hit.
PfamiPF01007. IRK. 1 hit.
PF08466. IRK_N. 1 hit.
[Graphical view]
PIRSFiPIRSF005465. GIRK_kir. 1 hit.
PRINTSiPR01325. KIR22CHANNEL.
PR01320. KIRCHANNEL.
SUPFAMiSSF81296. SSF81296. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and functional expression of cDNA encoding a second class of inward rectifier potassium channels in the mouse brain."
    Takahashi N., Morishige K., Jahangir A., Yamada M., Findlay I., Koyama H., Kurachi Y.
    J. Biol. Chem. 269:23274-23279(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Strain: BALB/c.
    Tissue: Brain.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Olfactory bulb.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  5. "Inward recifier potassium channel IRK2."
    Ikeda K., Kobayashi T.
    Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 10-420.
    Strain: C57BL/6NJcl.
    Tissue: Brain.

Entry informationi

Entry nameiKCJ12_MOUSE
AccessioniPrimary (citable) accession number: P52187
Secondary accession number(s): Q8CCR0, Q9QYF2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 3, 2012
Last modified: March 4, 2015
This is version 120 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.