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P52183

- ANNU_SCHAM

UniProt

P52183 - ANNU_SCHAM

Protein

Annulin

Gene
N/A
Organism
Schistocerca americana (American grasshopper)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 94 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Participates in morphogenetic activities of the cells, maybe by stabilizing the membrane or subcortical structures of cells that are under mechanical stress. Probably catalyzes the cross-linking of proteins and the conjugation of polyamines to proteins.

    Catalytic activityi

    Protein glutamine + alkylamine = protein N(5)-alkylglutamine + NH3.PROSITE-ProRule annotation

    Cofactori

    Binds 1 calcium ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei400 – 4001PROSITE-ProRule annotation
    Active sitei427 – 4271PROSITE-ProRule annotation
    Metal bindingi467 – 4671CalciumBy similarity
    Metal bindingi469 – 4691CalciumBy similarity
    Metal bindingi517 – 5171CalciumBy similarity
    Metal bindingi522 – 5221CalciumBy similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. protein-glutamine gamma-glutamyltransferase activity Source: UniProtKB-EC

    GO - Biological processi

    1. peptide cross-linking Source: InterPro

    Keywords - Molecular functioni

    Acyltransferase, Transferase

    Keywords - Ligandi

    Calcium, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Annulin
    Alternative name(s):
    Protein-glutamine gamma-glutamyltransferase (EC:2.3.2.13)
    Transglutaminase
    OrganismiSchistocerca americana (American grasshopper)
    Taxonomic identifieri7009 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraOrthopteroideaOrthopteraCaeliferaAcridomorphaAcridoideaAcrididaeCyrtacanthacridinaeSchistocerca

    Subcellular locationi

    Cell membrane; Lipid-anchor; Cytoplasmic side
    Note: Intracellular and peripherally associated with the inner leaflet of the cell membrane, using a fatty acid linkage.

    GO - Cellular componenti

    1. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 772772AnnulinPRO_0000213718Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi4 – 41S-palmitoyl cysteineSequence Analysis
    Lipidationi5 – 51S-palmitoyl cysteineSequence Analysis

    Keywords - PTMi

    Lipoprotein, Palmitate

    Expressioni

    Tissue specificityi

    Has an annular, or ring-like expression pattern in epithelial annuli of developing limb segment boundary cells. In embryos, it is seen in gastrulating cells, in cells surrounding rapidly dividing neuroblasts, and in muscle pioneer cells invaginating to form apodemes.

    Developmental stagei

    Expression of this protein in embryos and limbs is associated with areas undergoing movements, morphogenetic rearrangements, or rapid cell division. Expression of annulin precedes the first morphological signs of segmentation in the developing limbs.

    Structurei

    3D structure databases

    ProteinModelPortaliP52183.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Family and domain databases

    Gene3Di2.60.40.10. 3 hits.
    3.90.260.10. 1 hit.
    InterProiIPR023608. Gln_gamma-glutamylTfrase_euk.
    IPR013783. Ig-like_fold.
    IPR014756. Ig_E-set.
    IPR002931. Transglutaminase-like.
    IPR008958. Transglutaminase_C.
    IPR013808. Transglutaminase_CS.
    IPR001102. Transglutaminase_N.
    [Graphical view]
    PANTHERiPTHR11590. PTHR11590. 1 hit.
    PfamiPF00927. Transglut_C. 2 hits.
    PF01841. Transglut_core. 1 hit.
    PF00868. Transglut_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000459. TGM_EBP42. 1 hit.
    SMARTiSM00460. TGc. 1 hit.
    [Graphical view]
    SUPFAMiSSF49309. SSF49309. 2 hits.
    SSF81296. SSF81296. 1 hit.
    PROSITEiPS00547. TRANSGLUTAMINASES. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P52183-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGNCCSTFRA VFKPNEGSGG GIPLMPVRGG STRRPDSLPK PPAAVVPSPP    50
    SPGDVPDAGV APEVASVKEV DVLLAENGDA HRTRHYELMD REKEPRLVVR 100
    RGQPFAVSVT LSRPYNPDID AISFVFTVED AEKPSYGQGT LVAVPLLAKG 150
    AESGAAWNAV LDSSADDILR IQITPAADAI VGKWKMDIDT KLKNDGAVSY 200
    SYKDPFYILY NPWCRQDQVF LEGEELLQEY VLNDTGLIWR GSYNRLRPCV 250
    WKYAQFEKEI LDCALYLVSK IGGVRPSECG DPVRVCRAIS AAVNSPDDNG 300
    AVMGNWSNDY GGGTPPTKWI GSMKILQQFY KNKKPVKYGQ CWVFAGVLTT 350
    VCRALGLPAR TVTTYSAAHD TQNSLTVDYF VDDKGEIMEE MNSDSIWNFH 400
    VWTEVWMERP DLMPGDGAHY GGWQAVDSTP QELSDNMYRC GPAPVVAVKQ 450
    GEVLRPYDSA YVFAEVNADK VFWRYSGPTQ PLKLIRKDML GIGQNISTKA 500
    VGRFQREDIT NTYKYPEKSV EERAAMLKAL RQSESLFSRY YLNEDFNDIH 550
    FNFELRDDIV IGSPFSVVVV MKNRSNQQDY TVTVLLRVDT VLYTGHVKDG 600
    VKKEKVERLI KAGAVEEVRI DVSYEDYYKH LVDQCAFNIA CLATVHDTNY 650
    EYFAQDDFRV RKPDIKIKLE GEPVQGQEMS AVATLKNPLP IPVKKGQFLI 700
    EGPGIAKTQK IKLSQNIAPG EEASVNFKFT PKYDGRATIA AKFSSKELDD 750
    VDGFLNFMVE PKKEVNGTGN AA 772
    Length:772
    Mass (Da):85,942
    Last modified:October 1, 1996 - v1
    Checksum:iFA5A3CE6A7C4E394
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M92291 mRNA. Translation: AAA29806.1.
    PIRiA48822.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M92291 mRNA. Translation: AAA29806.1 .
    PIRi A48822.

    3D structure databases

    ProteinModelPortali P52183.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 2.60.40.10. 3 hits.
    3.90.260.10. 1 hit.
    InterProi IPR023608. Gln_gamma-glutamylTfrase_euk.
    IPR013783. Ig-like_fold.
    IPR014756. Ig_E-set.
    IPR002931. Transglutaminase-like.
    IPR008958. Transglutaminase_C.
    IPR013808. Transglutaminase_CS.
    IPR001102. Transglutaminase_N.
    [Graphical view ]
    PANTHERi PTHR11590. PTHR11590. 1 hit.
    Pfami PF00927. Transglut_C. 2 hits.
    PF01841. Transglut_core. 1 hit.
    PF00868. Transglut_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000459. TGM_EBP42. 1 hit.
    SMARTi SM00460. TGc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49309. SSF49309. 2 hits.
    SSF81296. SSF81296. 1 hit.
    PROSITEi PS00547. TRANSGLUTAMINASES. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Annulin, a protein expressed at limb segment boundaries in the grasshopper embryo, is homologous to protein cross-linking transglutaminases."
      Singer M.A., Hortsch M., Goodman C.S., Bentley D.
      Dev. Biol. 154:143-159(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].

    Entry informationi

    Entry nameiANNU_SCHAM
    AccessioniPrimary (citable) accession number: P52183
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 94 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3