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P52176

- MMP9_BOVIN

UniProt

P52176 - MMP9_BOVIN

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Protein
Matrix metalloproteinase-9
Gene
MMP9
Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Could play a role in bone osteoclastic resorption. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments By similarity.

Catalytic activityi

Cleavage of gelatin types I and V and collagen types IV and V.

Cofactori

Binds 2 zinc ions per subunit By similarity.
Binds 3 calcium ions per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi99 – 991Zinc 2; in inhibited form By similarity
Metal bindingi131 – 1311Calcium 1 By similarity
Metal bindingi165 – 1651Calcium 2; via carbonyl oxygen By similarity
Metal bindingi175 – 1751Zinc 1; structural By similarity
Metal bindingi177 – 1771Zinc 1; structural By similarity
Metal bindingi182 – 1821Calcium 3 By similarity
Metal bindingi183 – 1831Calcium 3; via carbonyl oxygen By similarity
Metal bindingi185 – 1851Calcium 3; via carbonyl oxygen By similarity
Metal bindingi187 – 1871Calcium 3; via carbonyl oxygen By similarity
Metal bindingi190 – 1901Zinc 1; structural By similarity
Metal bindingi197 – 1971Calcium 2; via carbonyl oxygen By similarity
Metal bindingi199 – 1991Calcium 2; via carbonyl oxygen By similarity
Metal bindingi201 – 2011Calcium 2 By similarity
Metal bindingi203 – 2031Zinc 1; structural By similarity
Metal bindingi205 – 2051Calcium 3 By similarity
Metal bindingi206 – 2061Calcium 1 By similarity
Metal bindingi208 – 2081Calcium 1 By similarity
Metal bindingi208 – 2081Calcium 3 By similarity
Metal bindingi401 – 4011Zinc 2; catalytic By similarity
Active sitei402 – 4021 By similarity
Metal bindingi405 – 4051Zinc 2; catalytic By similarity
Metal bindingi411 – 4111Zinc 2; catalytic By similarity

GO - Molecular functioni

  1. metalloendopeptidase activity Source: UniProtKB
  2. zinc ion binding Source: InterPro

GO - Biological processi

  1. collagen catabolic process Source: UniProtKB-KW
  2. leukocyte migration Source: InterPro
  3. ossification Source: InterPro
  4. proteolysis Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Biological processi

Collagen degradation

Keywords - Ligandi

Calcium, Metal-binding, Zinc

Protein family/group databases

MEROPSiM10.004.

Names & Taxonomyi

Protein namesi
Recommended name:
Matrix metalloproteinase-9 (EC:3.4.24.35)
Short name:
MMP-9
Alternative name(s):
92 kDa gelatinase
92 kDa type IV collagenase
Gelatinase B
Short name:
GELB
Gene namesi
Name:MMP9
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Unplaced

Subcellular locationi

GO - Cellular componenti

  1. extracellular space Source: UniProtKB
  2. proteinaceous extracellular matrix Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919 By similarity
Add
BLAST
Propeptidei20 – 10687Activation peptide
PRO_0000028750Add
BLAST
Chaini107 – 712606Matrix metalloproteinase-9
PRO_0000028751Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi38 – 381N-linked (GlcNAc...) Reviewed prediction
Glycosylationi120 – 1201N-linked (GlcNAc...) Reviewed prediction
Glycosylationi127 – 1271N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi230 ↔ 256 By similarity
Disulfide bondi244 ↔ 271 By similarity
Disulfide bondi288 ↔ 314 By similarity
Disulfide bondi302 ↔ 329 By similarity
Disulfide bondi347 ↔ 373 By similarity
Disulfide bondi361 ↔ 388 By similarity
Disulfide bondi521 ↔ 709 By similarity

Post-translational modificationi

N- and O-glycosylated By similarity.

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiP52176.
PRIDEiP52176.

Interactioni

Subunit structurei

Exists as monomer or homodimer; disulfide-linked By similarity. Exists also as heterodimer with a 25 kDa protein By similarity. Interacts with ECM1 By similarity.

Structurei

3D structure databases

ProteinModelPortaliP52176.
SMRiP52176. Positions 29-444, 518-712.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini225 – 27349Fibronectin type-II 1
Add
BLAST
Domaini283 – 33149Fibronectin type-II 2
Add
BLAST
Domaini342 – 39049Fibronectin type-II 3
Add
BLAST
Repeati523 – 56846Hemopexin 1
Add
BLAST
Repeati569 – 61345Hemopexin 2
Add
BLAST
Repeati615 – 66248Hemopexin 3
Add
BLAST
Repeati663 – 70947Hemopexin 4
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi97 – 1048Cysteine switch By similarity

Domaini

The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

Sequence similaritiesi

Belongs to the peptidase M10A family.
Contains 4 hemopexin repeats.

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiNOG328372.
HOGENOMiHOG000217926.
HOVERGENiHBG052484.
InParanoidiP52176.
KOiK01403.

Family and domain databases

Gene3Di2.10.10.10. 3 hits.
2.110.10.10. 1 hit.
3.40.390.10. 2 hits.
InterProiIPR000562. FN_type2_col-bd.
IPR000585. Hemopexin-like_dom.
IPR018487. Hemopexin-like_repeat.
IPR013806. Kringle-like.
IPR024079. MetalloPept_cat_dom.
IPR028688. MMP9.
IPR001818. Pept_M10_metallopeptidase.
IPR021190. Pept_M10A.
IPR021158. Pept_M10A_Zn_BS.
IPR006026. Peptidase_Metallo.
IPR002477. Peptidoglycan-bd-like.
IPR006970. PT.
[Graphical view]
PANTHERiPTHR10201:SF30. PTHR10201:SF30. 1 hit.
PfamiPF00040. fn2. 3 hits.
PF00045. Hemopexin. 3 hits.
PF00413. Peptidase_M10. 1 hit.
PF01471. PG_binding_1. 1 hit.
PF04886. PT. 1 hit.
[Graphical view]
PRINTSiPR00138. MATRIXIN.
SMARTiSM00059. FN2. 3 hits.
SM00120. HX. 4 hits.
SM00235. ZnMc. 1 hit.
[Graphical view]
SUPFAMiSSF47090. SSF47090. 1 hit.
SSF50923. SSF50923. 1 hit.
SSF57440. SSF57440. 3 hits.
PROSITEiPS00546. CYSTEINE_SWITCH. 1 hit.
PS00023. FN2_1. 3 hits.
PS51092. FN2_2. 3 hits.
PS51642. HEMOPEXIN_2. 4 hits.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P52176-1 [UniParc]FASTAAdd to Basket

« Hide

MSPLQPLVLA LLVLACCSAV PRRRQPTVVV FPGEPRTNLT NRQLAEEYLY    50
RYGYTPGAEL SEDGQSLQRA LLRFQRRLSL PETGELDSTT LNAMRAPRCG 100
VPDVGRFQTF EGELKWHHHN ITYWIQNYSE DLPRAVIDDA FARAFALWSA 150
VTPLTFTRVY GPEADIVIQF GVREHGDGYP FDGKNGLLAH AFPPGKGIQG 200
DAHFDDEELW SLGKGVVIPT YFGNAKGAAC HFPFTFEGRS YSACTTDGRS 250
DDMLWCSTTA DYDADRQFGF CPSERLYTQD GNADGKPCVF PFTFQGRTYS 300
ACTSDGRSDG YRWCATTANY DQDKLYGFCP TRVDATVTGG NAAGELCVFP 350
FTFLGKEYSA CTREGRNDGH LWCATTSNFD KDKKWGFCPD QGYSLFLVAA 400
HEFGHALGLD HTSVPEALMY PMYRFTEEHP LHRDDVQGIQ HLYGPRPEPE 450
PRPPTTTTTT TTEPQPTAPP TVCVTGPPTA RPSEGPTTGP TGPPAAGPTG 500
PPTAGPSAAP TESPDPAEDV CNVDIFDAIA EIRNRLHFFK AGKYWRLSEG 550
GGRRVQGPFL VKSKWPALPR KLDSAFEDPL TKKIFFFSGR QVWVYTGASL 600
LGPRRLDKLG LGPEVAQVTG ALPRPEGKVL LFSGQSFWRF DVKTQKVDPQ 650
SVTPVDQMFP GVPISTHDIF QYQEKAYFCQ DHFYWRVSSQ NEVNQVDYVG 700
YVTFDLLKCP ED 712
Length:712
Mass (Da):79,088
Last modified:October 1, 1996 - v1
Checksum:iE7FDA28372AEE0CE
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X78324 mRNA. Translation: CAA55127.1.
BC142430 mRNA. Translation: AAI42431.1.
PIRiI46031.
RefSeqiNP_777169.1. NM_174744.2.
UniGeneiBt.4714.

Genome annotation databases

GeneIDi282871.
KEGGibta:282871.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X78324 mRNA. Translation: CAA55127.1 .
BC142430 mRNA. Translation: AAI42431.1 .
PIRi I46031.
RefSeqi NP_777169.1. NM_174744.2.
UniGenei Bt.4714.

3D structure databases

ProteinModelPortali P52176.
SMRi P52176. Positions 29-444, 518-712.
ModBasei Search...
MobiDBi Search...

Chemistry

ChEMBLi CHEMBL5846.

Protein family/group databases

MEROPSi M10.004.

Proteomic databases

PaxDbi P52176.
PRIDEi P52176.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 282871.
KEGGi bta:282871.

Organism-specific databases

CTDi 4318.

Phylogenomic databases

eggNOGi NOG328372.
HOGENOMi HOG000217926.
HOVERGENi HBG052484.
InParanoidi P52176.
KOi K01403.

Miscellaneous databases

NextBioi 20806399.

Family and domain databases

Gene3Di 2.10.10.10. 3 hits.
2.110.10.10. 1 hit.
3.40.390.10. 2 hits.
InterProi IPR000562. FN_type2_col-bd.
IPR000585. Hemopexin-like_dom.
IPR018487. Hemopexin-like_repeat.
IPR013806. Kringle-like.
IPR024079. MetalloPept_cat_dom.
IPR028688. MMP9.
IPR001818. Pept_M10_metallopeptidase.
IPR021190. Pept_M10A.
IPR021158. Pept_M10A_Zn_BS.
IPR006026. Peptidase_Metallo.
IPR002477. Peptidoglycan-bd-like.
IPR006970. PT.
[Graphical view ]
PANTHERi PTHR10201:SF30. PTHR10201:SF30. 1 hit.
Pfami PF00040. fn2. 3 hits.
PF00045. Hemopexin. 3 hits.
PF00413. Peptidase_M10. 1 hit.
PF01471. PG_binding_1. 1 hit.
PF04886. PT. 1 hit.
[Graphical view ]
PRINTSi PR00138. MATRIXIN.
SMARTi SM00059. FN2. 3 hits.
SM00120. HX. 4 hits.
SM00235. ZnMc. 1 hit.
[Graphical view ]
SUPFAMi SSF47090. SSF47090. 1 hit.
SSF50923. SSF50923. 1 hit.
SSF57440. SSF57440. 3 hits.
PROSITEi PS00546. CYSTEINE_SWITCH. 1 hit.
PS00023. FN2_1. 3 hits.
PS51092. FN2_2. 3 hits.
PS51642. HEMOPEXIN_2. 4 hits.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Infection with Theileria annulata induces expression of matrix metalloproteinase 9 and transcription factor AP-1 in bovine leucocytes."
    Baylis H.A., Megson A., Hall R.
    Mol. Biochem. Parasitol. 69:211-222(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Leukocyte.
  2. NIH - Mammalian Gene Collection (MGC) project
    Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Crossbred X Angus.
    Tissue: Ileum.

Entry informationi

Entry nameiMMP9_BOVIN
AccessioniPrimary (citable) accession number: P52176
Secondary accession number(s): A5PKB8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 11, 2014
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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