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Protein

Nucleoside diphosphate kinase A 2

Gene

NME1-2

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Major role in the synthesis of nucleoside triphosphates other than ATP. Possesses nucleoside-diphosphate kinase, serine/threonine-specific protein kinase, geranyl and farnesyl pyrophosphate kinase, histidine protein kinase and 3'-5' exonuclease activities. Involved in cell proliferation, differentiation and development, signal transduction, G protein-coupled receptor endocytosis, and gene expression. Required for neural development including neural patterning and cell fate determination.

Catalytic activityi

ATP + nucleoside diphosphate = ADP + nucleoside triphosphate.1 Publication

Cofactori

Mg2+By similarity

Enzyme regulationi

Autophosphorylation at His-118 increases serine/threonine protein kinase activity of the enzyme. Interaction with the SET complex inhibits exonuclease activity (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei12ATP1
Binding sitei60ATP1
Binding sitei88ATP1
Binding sitei94ATP1
Binding sitei105ATP1
Binding sitei115ATP1
Active sitei118Pros-phosphohistidine intermediate1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Differentiation, Endocytosis, Neurogenesis, Nucleotide metabolism

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleoside diphosphate kinase A 2 (EC:2.7.4.6)
Short name:
NDK A 2
Short name:
NDP kinase A 2
Alternative name(s):
Nucleoside diphosphate kinase NBR-B
Short name:
NDK NBR-B
Gene namesi
Name:NME1-2
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001371122 – 152Nucleoside diphosphate kinase A 2Add BLAST151

Post-translational modificationi

The N-terminus is blocked.

Proteomic databases

PaxDbiP52175.
PRIDEiP52175.

Interactioni

Subunit structurei

Homohexamer.1 Publication

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000006106.

Structurei

Secondary structure

1152
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi6 – 11Combined sources6
Helixi13 – 17Combined sources5
Helixi21 – 31Combined sources11
Beta strandi34 – 41Combined sources8
Helixi45 – 52Combined sources8
Helixi53 – 55Combined sources3
Beta strandi56 – 58Combined sources3
Helixi61 – 63Combined sources3
Helixi64 – 69Combined sources6
Beta strandi73 – 80Combined sources8
Helixi83 – 91Combined sources9
Helixi96 – 98Combined sources3
Helixi104 – 108Combined sources5
Beta strandi117 – 119Combined sources3
Helixi123 – 133Combined sources11
Helixi136 – 138Combined sources3
Helixi147 – 150Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BE4X-ray2.40A/B/C2-152[»]
ProteinModelPortaliP52175.
SMRiP52175.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP52175.

Family & Domainsi

Sequence similaritiesi

Belongs to the NDK family.Curated

Phylogenomic databases

eggNOGiKOG0888. Eukaryota.
COG0105. LUCA.
HOVERGENiHBG000423.
InParanoidiP52175.

Family and domain databases

HAMAPiMF_00451. NDP_kinase. 1 hit.
InterProiIPR001564. Nucleoside_diP_kinase.
IPR023005. Nucleoside_diP_kinase_AS.
[Graphical view]
PfamiPF00334. NDK. 1 hit.
[Graphical view]
PRINTSiPR01243. NUCDPKINASE.
SMARTiSM00562. NDK. 1 hit.
[Graphical view]
PROSITEiPS00469. NDP_KINASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P52175-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANSERTFIA IKPDGVQRGL MGEIIKRFEQ KGFRLVAMKF MRASEDLLKE
60 70 80 90 100
HYIDLKDRPF FAGLVKYMHS GPVVAMVWEG LNVVKTGRVM LGETNPADSK
110 120 130 140 150
PGTIRGDFCI QVGRNIIHGS DSVESAEKEI ALWFRPEELV NYKSCAQNWI

YE
Length:152
Mass (Da):17,298
Last modified:January 23, 2007 - v3
Checksum:iF710E1DFC731B04B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X92957 mRNA. Translation: CAA63533.1.
UniGeneiBt.5340.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X92957 mRNA. Translation: CAA63533.1.
UniGeneiBt.5340.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BE4X-ray2.40A/B/C2-152[»]
ProteinModelPortaliP52175.
SMRiP52175.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000006106.

Proteomic databases

PaxDbiP52175.
PRIDEiP52175.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiKOG0888. Eukaryota.
COG0105. LUCA.
HOVERGENiHBG000423.
InParanoidiP52175.

Miscellaneous databases

EvolutionaryTraceiP52175.

Family and domain databases

HAMAPiMF_00451. NDP_kinase. 1 hit.
InterProiIPR001564. Nucleoside_diP_kinase.
IPR023005. Nucleoside_diP_kinase_AS.
[Graphical view]
PfamiPF00334. NDK. 1 hit.
[Graphical view]
PRINTSiPR01243. NUCDPKINASE.
SMARTiSM00562. NDK. 1 hit.
[Graphical view]
PROSITEiPS00469. NDP_KINASES. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNDKA2_BOVIN
AccessioniPrimary (citable) accession number: P52175
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 108 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.