Reviewed, UniProtKB/Swiss-Prot P52175 (NDKA2_BOVIN)

Names and origin

Protein names

Recommended name:
    Nucleoside diphosphate kinase A 2
      Short name=NDP kinase A 2
      Short name=NDK A 2
    EC=2.7.4.6
Alternative name(s):
    Nucleoside diphosphate kinase NBR-B
      Short name=NDK NBR-B

Gene names

Name:

NME1-2

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	152 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Major role in the synthesis of nucleoside triphosphates other than ATP. Possesses nucleoside-diphosphate kinase, serine/threonine-specific protein kinase, geranyl and farnesyl pyrophosphate kinase, histidine protein kinase and 3'-5' exonuclease activities. Involved in cell proliferation, differentiation and development, signal transduction, G protein-coupled receptor endocytosis, and gene expression. Required for neural development including neural patterning and cell fate determination.
Catalytic activity	ATP + nucleoside diphosphate = ADP + nucleoside triphosphate. Ref.1
Cofactor	Magnesium By similarity.
Enzyme regulation	Autophosphorylation at His-118 increases serine/threonine protein kinase activity of the enzyme. Interaction with the SET complex inhibits exonuclease activity By similarity.
Subunit structure	Homohexamer. Ref.1
Subcellular location	Cytoplasm. Cell membrane. Nucleus By similarity.
Post-translational modification	The N-terminus is blocked.
Sequence similarities	Belongs to the NDK family.

Ontologies

Keywords
Biological process	Differentiation Endocytosis Neurogenesis Nucleotide metabolism
Cellular component	Cell membrane Cytoplasm Membrane Nucleus
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	CTP biosynthetic process Inferred from electronic annotation. Source: InterPro GTP biosynthetic process Inferred from electronic annotation. Source: InterPro UTP biosynthetic process Inferred from electronic annotation. Source: InterPro cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW endocytosis Inferred from electronic annotation. Source: UniProtKB-KW nervous system development Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside diphosphate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.1

Chain

2 – 152

151

Nucleoside diphosphate kinase A 2

PRO_0000137112

Sites

Active site

118

1

Pros-phosphohistidine intermediate

Binding site

12

1

ATP

Binding site

60

1

ATP

Binding site

88

1

ATP

Binding site

94

1

ATP

Binding site

105

1

ATP

Binding site

115

1

ATP

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

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152

Helix Strand Turn

Details...

Beta strand

6 – 11

6

Helix

13 – 17

5

Helix

21 – 31

11

Beta strand

34 – 41

8

Helix

45 – 52

8

Helix

53 – 55

3

Beta strand

56 – 58

3

Helix

61 – 63

3

Helix

64 – 69

6

Beta strand

73 – 80

8

Helix

83 – 91

9

Helix

96 – 98

3

Helix

104 – 108

5

Beta strand

117 – 119

3

Helix

123 – 133

11

Helix

136 – 138

3

Helix

147 – 150

4

Sequences

	Sequence		Length	Mass (Da)
	P52175-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: F710E1DFC731B04B	FASTA	152	17,298
	10 20 30 40 50 60 MANSERTFIA IKPDGVQRGL MGEIIKRFEQ KGFRLVAMKF MRASEDLLKE HYIDLKDRPF 70 80 90 100 110 120 FAGLVKYMHS GPVVAMVWEG LNVVKTGRVM LGETNPADSK PGTIRGDFCI QVGRNIIHGS 130 140 150 DSVESAEKEI ALWFRPEELV NYKSCAQNWI YE

References

[1]

"Nucleoside diphosphate kinase from bovine retina: purification, subcellular localization, molecular cloning, and three-dimensional structure." Abdulaev N.G., Karaschuk G.N., Ladner J.E., Kakuev D.L., Yakhyaev A.V., Tordova M., Gaidarov I.O., Popov V.I., Fujiwara J.H., Chinchilla D., Eisenstein E., Gilliland G.L., Ridge K.D. Biochemistry 37:13958-13967(1998) [PubMed: 9760230] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 2-152, CATALYTIC ACTIVITY, SUBUNIT, SUBCELLULAR LOCATION, BLOCKAGE OF N-TERMINUS, X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS). Tissue: Retina.

Cross-references

Sequence databases

EMBLGenBankDDBJ

X92957 mRNA. Translation: CAA63533.1.

IPI

IPI00711386.

UniGene

Bt.5340

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1BE4	X-ray	2.40	A/B/C	2-152	[»]

ModBase

Search...

Protein-protein interaction databases

STRING

P52175.

Genome annotation databases

Ensembl

ENSBTAT00000006104; ENSBTAP00000006104; ENSBTAG00000004651; Bos taurus. [Genome view]
ENSBTAT00000006106; ENSBTAP00000006106; ENSBTAG00000004651; Bos taurus. [Genome view]
ENSBTAT00000043502; ENSBTAP00000041066; ENSBTAG00000004651; Bos taurus. [Genome view]

Phylogenomic databases

HOVERGEN

P52175.

Enzyme and pathway databases

BRENDA

2.7.4.6. 251.

Family and domain databases

InterPro

IPR001564. Nuc_diP_kinase_core.
[Graphical view]

Gene3D

G3DSA:3.30.70.141. NDK. 1 hit.

PANTHER

PTHR11349. Nuc_diP_kinase_core. 1 hit.

Pfam

PF00334. NDK. 1 hit.
[Graphical view]

PRINTS

PR01243. NUCDPKINASE.

ProDom

PD001018. NDK. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SMART

SM00562. NDK. 1 hit.
[Graphical view]

PROSITE

PS00469. NDP_KINASES. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

NDKA2_BOVIN

Accession

Primary (citable) accession number: P52175

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	January 23, 2007
Last modified:	September 1, 2009
This is version 71 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)