Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Nucleoside diphosphate kinase A 1

Gene

NME1-1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Major role in the synthesis of nucleoside triphosphates other than ATP. Possesses nucleoside-diphosphate kinase, serine/threonine-specific protein kinase, geranyl and farnesyl pyrophosphate kinase, histidine protein kinase and 3'-5' exonuclease activities. Involved in cell proliferation, differentiation and development, signal transduction, G protein-coupled receptor endocytosis, and gene expression. Required for neural development including neural patterning and cell fate determination.

Catalytic activityi

ATP + nucleoside diphosphate = ADP + nucleoside triphosphate.1 Publication

Cofactori

Mg2+By similarity

Enzyme regulationi

Autophosphorylation at His-118 increases serine/threonine protein kinase activity of the enzyme. Interaction with the SET complex inhibits exonuclease activity (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei12 – 121ATP
Binding sitei60 – 601ATP
Binding sitei88 – 881ATP
Binding sitei94 – 941ATP
Binding sitei105 – 1051ATP
Binding sitei115 – 1151ATP
Active sitei118 – 1181Pros-phosphohistidine intermediate

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Differentiation, Endocytosis, Neurogenesis, Nucleotide metabolism

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleoside diphosphate kinase A 1 (EC:2.7.4.6)
Short name:
NDK A 1
Short name:
NDP kinase A 1
Alternative name(s):
Nucleoside diphosphate kinase NBR-A
Short name:
NDK NBR-A
Gene namesi
Name:NME1-1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 152151Nucleoside diphosphate kinase A 1PRO_0000137111Add
BLAST

Post-translational modificationi

The N-terminus is blocked.

Proteomic databases

PaxDbiP52174.
PRIDEiP52174.

Interactioni

Subunit structurei

Homohexamer.1 Publication

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000006106.

Structurei

Secondary structure

1
152
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi6 – 116Combined sources
Helixi13 – 175Combined sources
Helixi21 – 3010Combined sources
Beta strandi34 – 429Combined sources
Helixi45 – 517Combined sources
Helixi53 – 553Combined sources
Helixi61 – 7010Combined sources
Beta strandi71 – 8010Combined sources
Helixi83 – 919Combined sources
Turni96 – 983Combined sources
Helixi104 – 1085Combined sources
Turni112 – 1143Combined sources
Beta strandi117 – 1193Combined sources
Helixi123 – 13311Combined sources
Helixi136 – 1383Combined sources
Helixi147 – 1504Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1BHNX-ray2.40A/B/C/D/E/F1-152[»]
ProteinModelPortaliP52174.
SMRiP52174. Positions 2-152.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP52174.

Family & Domainsi

Sequence similaritiesi

Belongs to the NDK family.Curated

Phylogenomic databases

eggNOGiKOG0888. Eukaryota.
COG0105. LUCA.
HOGENOMiHOG000224564.
HOVERGENiHBG000423.
InParanoidiP52174.

Family and domain databases

HAMAPiMF_00451. NDP_kinase.
InterProiIPR001564. Nucleoside_diP_kinase.
IPR023005. Nucleoside_diP_kinase_AS.
[Graphical view]
PfamiPF00334. NDK. 1 hit.
[Graphical view]
PRINTSiPR01243. NUCDPKINASE.
SMARTiSM00562. NDK. 1 hit.
[Graphical view]
PROSITEiPS00469. NDP_KINASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P52174-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANSERTFIA IKPDGVQRGL IGEIIKRFEQ KGFRLVAMKF MRASEDLLKE
60 70 80 90 100
HYIDLKDRPF FAGLVKYMHS GPVVAMVWEG LNVVKTGRVM LGETNPADSK
110 120 130 140 150
PGTIRGDFCI QVGRNIIHGS DSVESAEKEI ALWFHPEELV NYKSCAQNWI

YE
Length:152
Mass (Da):17,261
Last modified:January 23, 2007 - v3
Checksum:iF7052FBC1337704B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X92956 mRNA. Translation: CAA63532.1.
UniGeneiBt.5340.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X92956 mRNA. Translation: CAA63532.1.
UniGeneiBt.5340.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1BHNX-ray2.40A/B/C/D/E/F1-152[»]
ProteinModelPortaliP52174.
SMRiP52174. Positions 2-152.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000006106.

Proteomic databases

PaxDbiP52174.
PRIDEiP52174.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiKOG0888. Eukaryota.
COG0105. LUCA.
HOGENOMiHOG000224564.
HOVERGENiHBG000423.
InParanoidiP52174.

Miscellaneous databases

EvolutionaryTraceiP52174.

Family and domain databases

HAMAPiMF_00451. NDP_kinase.
InterProiIPR001564. Nucleoside_diP_kinase.
IPR023005. Nucleoside_diP_kinase_AS.
[Graphical view]
PfamiPF00334. NDK. 1 hit.
[Graphical view]
PRINTSiPR01243. NUCDPKINASE.
SMARTiSM00562. NDK. 1 hit.
[Graphical view]
PROSITEiPS00469. NDP_KINASES. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Nucleoside diphosphate kinase from bovine retina: purification, subcellular localization, molecular cloning, and three-dimensional structure."
    Abdulaev N.G., Karaschuk G.N., Ladner J.E., Kakuev D.L., Yakhyaev A.V., Tordova M., Gaidarov I.O., Popov V.I., Fujiwara J.H., Chinchilla D., Eisenstein E., Gilliland G.L., Ridge K.D.
    Biochemistry 37:13958-13967(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 2-152, CATALYTIC ACTIVITY, SUBUNIT, SUBCELLULAR LOCATION, BLOCKAGE OF N-TERMINUS, X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS).
    Tissue: Retina.

Entry informationi

Entry nameiNDKA1_BOVIN
AccessioniPrimary (citable) accession number: P52174
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: January 23, 2007
Last modified: February 17, 2016
This is version 106 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.