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Reviewed, UniProtKB/Swiss-Prot P52174 (NDKA1_BOVIN)

Last modified September 1, 2009. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Nucleoside diphosphate kinase A 1
      Short name=NDP kinase A 1
      Short name=NDK A 1
    EC=2.7.4.6
Alternative name(s):
    Nucleoside diphosphate kinase NBR-A
      Short name=NDK NBR-A
Gene names
Name: NME1-1
OrganismBos taurus (Bovine)
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

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Protein attributes

Sequence length152 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Major role in the synthesis of nucleoside triphosphates other than ATP. Possesses nucleoside-diphosphate kinase, serine/threonine-specific protein kinase, geranyl and farnesyl pyrophosphate kinase, histidine protein kinase and 3'-5' exonuclease activities. Involved in cell proliferation, differentiation and development, signal transduction, G protein-coupled receptor endocytosis, and gene expression. Required for neural development including neural patterning and cell fate determination.

Catalytic activity

ATP + nucleoside diphosphate = ADP + nucleoside triphosphate. Ref.1

Cofactor

Magnesium By similarity.

Enzyme regulation

Autophosphorylation at His-118 increases serine/threonine protein kinase activity of the enzyme. Interaction with the SET complex inhibits exonuclease activity By similarity.

Subunit structure

Homohexamer. Ref.1

Subcellular location

Cytoplasm. Cell membrane. Nucleus By similarity.

Post-translational modification

The N-terminus is blocked.

Sequence similarities

Belongs to the NDK family.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.1
Chain2 – 152151Nucleoside diphosphate kinase A 1
PRO_0000137111

Sites

Active site1181Pros-phosphohistidine intermediate
Binding site121ATP
Binding site601ATP
Binding site881ATP
Binding site941ATP
Binding site1051ATP
Binding site1151ATP

Secondary structure

1 ................................ 152
Helix Strand Turn

Details...

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Sequences

Sequence LengthMass (Da)Tools
P52174-1 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: F7052FBC1337704B

FASTA15217,261
        10         20         30         40         50         60 
MANSERTFIA IKPDGVQRGL IGEIIKRFEQ KGFRLVAMKF MRASEDLLKE HYIDLKDRPF 

        70         80         90        100        110        120 
FAGLVKYMHS GPVVAMVWEG LNVVKTGRVM LGETNPADSK PGTIRGDFCI QVGRNIIHGS 

       130        140        150 
DSVESAEKEI ALWFHPEELV NYKSCAQNWI YE

« Hide

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References

[1]"Nucleoside diphosphate kinase from bovine retina: purification, subcellular localization, molecular cloning, and three-dimensional structure."
Abdulaev N.G., Karaschuk G.N., Ladner J.E., Kakuev D.L., Yakhyaev A.V., Tordova M., Gaidarov I.O., Popov V.I., Fujiwara J.H., Chinchilla D., Eisenstein E., Gilliland G.L., Ridge K.D.
Biochemistry 37:13958-13967(1998) [PubMed: 9760230] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 2-152, CATALYTIC ACTIVITY, SUBUNIT, SUBCELLULAR LOCATION, BLOCKAGE OF N-TERMINUS, X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS).
Tissue: Retina.
+Additional computationally mapped references.

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Cross-references

Sequence databases

X92956 mRNA. Translation: CAA63532.1.
IPIIPI00688948.
UniGeneBt.5340

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
1BHNX-ray2.40A/B/C/D/E/F2-151[»]
ModBaseSearch...

Protein-protein interaction databases

STRINGP52174.

Genome annotation databases

EnsemblENSBTAT00000006104; ENSBTAP00000006104; ENSBTAG00000004651; Bos taurus. [Genome view]
ENSBTAT00000006106; ENSBTAP00000006106; ENSBTAG00000004651; Bos taurus. [Genome view]
ENSBTAT00000043502; ENSBTAP00000041066; ENSBTAG00000004651; Bos taurus. [Genome view]

Phylogenomic databases

HOVERGENP52174.

Enzyme and pathway databases

BRENDA2.7.4.6. 251.

Family and domain databases

InterProIPR001564. Nuc_diP_kinase_core.
[Graphical view]
Gene3DG3DSA:3.30.70.141. NDK. 1 hit.
PANTHERPTHR11349. Nuc_diP_kinase_core. 1 hit.
PfamPF00334. NDK. 1 hit.
[Graphical view]
PRINTSPR01243. NUCDPKINASE.
ProDomPD001018. NDK. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00562. NDK. 1 hit.
[Graphical view]
PROSITEPS00469. NDP_KINASES. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameNDKA1_BOVIN
AccessionPrimary (citable) accession number: P52174
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: January 23, 2007
Last modified: September 1, 2009
This is version 68 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents