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Protein

GATA-binding factor A

Gene

pnr

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional regulator involved in several developmental processes during embryonic and imaginal disks development. Involved in determining dorsal cell fate. Acts as an essential transcriptional regulator of proneural achaete-scute complex (AS-C) and is required for its spatial regulation during development of the adult peripheral nervous system, and hence for the positioning of neural precursors. It is the only factor to directly activate AS-C genes.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri169 – 193GATA-type 1PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri226 – 250GATA-type 2PROSITE-ProRule annotationAdd BLAST25

GO - Molecular functioni

GO - Biological processi

  • anatomical structure formation involved in morphogenesis Source: GO_Central
  • blastoderm segmentation Source: FlyBase
  • cardioblast cell fate determination Source: FlyBase
  • cardioblast differentiation Source: FlyBase
  • cardiocyte differentiation Source: FlyBase
  • cell development Source: GO_Central
  • cell differentiation Source: FlyBase
  • chaeta development Source: FlyBase
  • chaeta morphogenesis Source: FlyBase
  • digestive tract development Source: GO_Central
  • dorsal closure Source: FlyBase
  • ectoderm development Source: FlyBase
  • embryonic heart tube development Source: FlyBase
  • heart contraction Source: FlyBase
  • heart development Source: FlyBase
  • lymph gland development Source: FlyBase
  • negative regulation of transcription, DNA-templated Source: FlyBase
  • negative regulation of transcription from RNA polymerase II promoter Source: FlyBase
  • pattern specification process Source: FlyBase
  • pericardial nephrocyte differentiation Source: FlyBase
  • pigment metabolic process Source: FlyBase
  • positive regulation of transcription, DNA-templated Source: FlyBase
  • positive regulation of transcription from RNA polymerase II promoter Source: FlyBase
  • regulation of antimicrobial peptide biosynthetic process Source: FlyBase
  • regulation of glucose metabolic process Source: FlyBase
  • transforming growth factor beta receptor signaling pathway Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-DME-5683826. Surfactant metabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
GATA-binding factor A
Alternative name(s):
Protein pannier
Transcription factor GATA-A
dGATA-A
Gene namesi
Name:pnr
Synonyms:GATA-A
ORF Names:CG3978
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0003117. pnr.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: FlyBase
  • transcription factor complex Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi168E → K in pnrD4; abolishes DNA-binding. 1
Mutagenesisi169C → Y in pnrD1; abolishes DNA-binding. 1
Mutagenesisi186G → E in pnrD2; abolishes DNA-binding. 1
Mutagenesisi190C → S in pnrD3; abolishes DNA-binding. 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000834601 – 540GATA-binding factor AAdd BLAST540

Proteomic databases

PaxDbiP52168.
PRIDEiP52168.

Expressioni

Developmental stagei

It is first seen in the dorsal portion of the embryo just after cellularization and is expressed at high levels during early embryogenesis and as development progresses high levels are seen in the dorsal epidermis.

Gene expression databases

BgeeiFBgn0003117.
ExpressionAtlasiP52168. baseline.
GenevisibleiP52168. DM.

Interactioni

Subunit structurei

Interacts with OSA.1 Publication

GO - Molecular functioni

  • protein heterodimerization activity Source: FlyBase
  • RNA polymerase II repressing transcription factor binding Source: FlyBase
  • RNA polymerase II transcription factor binding Source: FlyBase

Protein-protein interaction databases

BioGridi69326. 66 interactors.
STRINGi7227.FBpp0082675.

Structurei

3D structure databases

ProteinModelPortaliP52168.
SMRiP52168.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi333 – 336Poly-Ser4
Compositional biasi345 – 364Poly-GlnAdd BLAST20
Compositional biasi448 – 451Poly-His4
Compositional biasi462 – 465Poly-Ala4
Compositional biasi471 – 477Poly-Ala7

Sequence similaritiesi

Contains 2 GATA-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri169 – 193GATA-type 1PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri226 – 250GATA-type 2PROSITE-ProRule annotationAdd BLAST25

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1601. Eukaryota.
COG5641. LUCA.
GeneTreeiENSGT00760000119221.
InParanoidiP52168.
KOiK09183.
OMAiYESAMDF.
OrthoDBiEOG091G0AUR.
PhylomeDBiP52168.

Family and domain databases

Gene3Di3.30.50.10. 2 hits.
InterProiIPR028436. GATA_4/pnr.
IPR000679. Znf_GATA.
IPR013088. Znf_NHR/GATA.
[Graphical view]
PANTHERiPTHR10071:SF154. PTHR10071:SF154. 1 hit.
PfamiPF00320. GATA. 2 hits.
[Graphical view]
PRINTSiPR00619. GATAZNFINGER.
SMARTiSM00401. ZnF_GATA. 2 hits.
[Graphical view]
PROSITEiPS00344. GATA_ZN_FINGER_1. 2 hits.
PS50114. GATA_ZN_FINGER_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P52168-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGILLSDGDS TSDQQSTRDY PHFSGDYQNV TLSAASASTS ASASATHVAA
60 70 80 90 100
VKMYHSSAVA AYTDLAAAGS AASAGVGVGV SGYHQQAVNA PVYVPSNRQY
110 120 130 140 150
NHVAAHFGSA AAQNAWTTEG FGSAHAQFYS PNAAVMMGSW RSAYDPSGFQ
160 170 180 190 200
RSSPYESAMD FQFGEGRECV NCGAISTPLW RRDGTGHYLC NACGLYHKMN
210 220 230 240 250
GMNRPLIKPS KRLVSATATR RMGLCCTNCG TRTTTLWRRN NDGEPVCNAC
260 270 280 290 300
GLYYKLHGVN RPLAMRKDGI QTRKRKPKKT GSGSAVGAGT GSGTGSTLEA
310 320 330 340 350
IKECKEEHDL KPSLSLERHS LSKLHTDMKS GTSSSSTLMG HHSAQQQQQQ
360 370 380 390 400
QQQQQQQQQQ QQQQSAHQQC FPLYGQTTTQ QQHQQHGHSM TSSSGQAHLS
410 420 430 440 450
ARHLHGAAGT QLYTPGSSSG GGSASAYTSH SAETPALSNG TPSPHYQHHH
460 470 480 490 500
HLGGTHGHHV TAAAAHHHFH AAAAVAAYGV KTEASATNYD YVNNCYFGGT
510 520 530 540
FGALGGAATT TAMAGGAASE LAGYHHQHNV IQAAKLMATS
Length:540
Mass (Da):57,036
Last modified:October 1, 1996 - v1
Checksum:i46557C0754BEEB59
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti413Y → S in AAB29876 (PubMed:7916679).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S68798
, S68909, S68793, S68795 Genomic DNA. Translation: AAB29874.1.
S68803, S68802 Genomic DNA. Translation: AAB29876.2.
AE014297 Genomic DNA. Translation: AAN13693.1.
RefSeqiNP_476685.1. NM_057337.3.
UniGeneiDm.7432.

Genome annotation databases

EnsemblMetazoaiFBtr0083221; FBpp0082675; FBgn0003117.
GeneIDi44849.
KEGGidme:Dmel_CG3978.
UCSCiCG3978-RA. d. melanogaster.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S68798
, S68909, S68793, S68795 Genomic DNA. Translation: AAB29874.1.
S68803, S68802 Genomic DNA. Translation: AAB29876.2.
AE014297 Genomic DNA. Translation: AAN13693.1.
RefSeqiNP_476685.1. NM_057337.3.
UniGeneiDm.7432.

3D structure databases

ProteinModelPortaliP52168.
SMRiP52168.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi69326. 66 interactors.
STRINGi7227.FBpp0082675.

Proteomic databases

PaxDbiP52168.
PRIDEiP52168.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0083221; FBpp0082675; FBgn0003117.
GeneIDi44849.
KEGGidme:Dmel_CG3978.
UCSCiCG3978-RA. d. melanogaster.

Organism-specific databases

CTDi44849.
FlyBaseiFBgn0003117. pnr.

Phylogenomic databases

eggNOGiKOG1601. Eukaryota.
COG5641. LUCA.
GeneTreeiENSGT00760000119221.
InParanoidiP52168.
KOiK09183.
OMAiYESAMDF.
OrthoDBiEOG091G0AUR.
PhylomeDBiP52168.

Enzyme and pathway databases

ReactomeiR-DME-5683826. Surfactant metabolism.

Miscellaneous databases

GenomeRNAii44849.
PROiP52168.

Gene expression databases

BgeeiFBgn0003117.
ExpressionAtlasiP52168. baseline.
GenevisibleiP52168. DM.

Family and domain databases

Gene3Di3.30.50.10. 2 hits.
InterProiIPR028436. GATA_4/pnr.
IPR000679. Znf_GATA.
IPR013088. Znf_NHR/GATA.
[Graphical view]
PANTHERiPTHR10071:SF154. PTHR10071:SF154. 1 hit.
PfamiPF00320. GATA. 2 hits.
[Graphical view]
PRINTSiPR00619. GATAZNFINGER.
SMARTiSM00401. ZnF_GATA. 2 hits.
[Graphical view]
PROSITEiPS00344. GATA_ZN_FINGER_1. 2 hits.
PS50114. GATA_ZN_FINGER_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPNR_DROME
AccessioniPrimary (citable) accession number: P52168
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 30, 2016
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.