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Protein

GATA-binding factor A

Gene

pnr

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional regulator involved in several developmental processes during embryonic and imaginal disks development. Involved in determining dorsal cell fate. Acts as an essential transcriptional regulator of proneural achaete-scute complex (AS-C) and is required for its spatial regulation during development of the adult peripheral nervous system, and hence for the positioning of neural precursors. It is the only factor to directly activate AS-C genes.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri169 – 19325GATA-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri226 – 25025GATA-type 2PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • protein heterodimerization activity Source: FlyBase
  • RNA polymerase II repressing transcription factor binding Source: FlyBase
  • RNA polymerase II transcription factor activity, sequence-specific DNA binding Source: FlyBase
  • RNA polymerase II transcription factor binding Source: FlyBase
  • sequence-specific DNA binding Source: FlyBase
  • transcription factor activity, RNA polymerase II core promoter sequence-specific Source: FlyBase
  • transcription factor activity, sequence-specific DNA binding Source: FlyBase
  • zinc ion binding Source: InterPro

GO - Biological processi

  • blastoderm segmentation Source: FlyBase
  • cardioblast cell fate determination Source: FlyBase
  • cardioblast differentiation Source: FlyBase
  • cardiocyte differentiation Source: FlyBase
  • cell differentiation Source: FlyBase
  • chaeta development Source: FlyBase
  • chaeta morphogenesis Source: FlyBase
  • dorsal closure Source: FlyBase
  • ectoderm development Source: FlyBase
  • embryonic heart tube development Source: FlyBase
  • heart contraction Source: FlyBase
  • heart development Source: FlyBase
  • lymph gland development Source: FlyBase
  • mesoderm development Source: FlyBase
  • negative regulation of transcription, DNA-templated Source: FlyBase
  • negative regulation of transcription from RNA polymerase II promoter Source: FlyBase
  • pattern specification process Source: FlyBase
  • pericardial nephrocyte differentiation Source: FlyBase
  • pigment metabolic process Source: FlyBase
  • positive regulation of transcription, DNA-templated Source: FlyBase
  • positive regulation of transcription from RNA polymerase II promoter Source: FlyBase
  • regulation of antimicrobial peptide biosynthetic process Source: FlyBase
  • regulation of glucose metabolic process Source: FlyBase
  • transcription, DNA-templated Source: UniProtKB-KW
  • transforming growth factor beta receptor signaling pathway Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-DME-5683826. Surfactant metabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
GATA-binding factor A
Alternative name(s):
Protein pannier
Transcription factor GATA-A
dGATA-A
Gene namesi
Name:pnr
Synonyms:GATA-A
ORF Names:CG3978
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0003117. pnr.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi168 – 1681E → K in pnrD4; abolishes DNA-binding.
Mutagenesisi169 – 1691C → Y in pnrD1; abolishes DNA-binding.
Mutagenesisi186 – 1861G → E in pnrD2; abolishes DNA-binding.
Mutagenesisi190 – 1901C → S in pnrD3; abolishes DNA-binding.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 540540GATA-binding factor APRO_0000083460Add
BLAST

Proteomic databases

PaxDbiP52168.
PRIDEiP52168.

Expressioni

Developmental stagei

It is first seen in the dorsal portion of the embryo just after cellularization and is expressed at high levels during early embryogenesis and as development progresses high levels are seen in the dorsal epidermis.

Gene expression databases

BgeeiP52168.
ExpressionAtlasiP52168. differential.
GenevisibleiP52168. DM.

Interactioni

Subunit structurei

Interacts with OSA.1 Publication

GO - Molecular functioni

  • protein heterodimerization activity Source: FlyBase
  • RNA polymerase II repressing transcription factor binding Source: FlyBase
  • RNA polymerase II transcription factor binding Source: FlyBase

Protein-protein interaction databases

BioGridi69326. 66 interactions.
STRINGi7227.FBpp0082675.

Structurei

3D structure databases

ProteinModelPortaliP52168.
SMRiP52168. Positions 165-276.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi333 – 3364Poly-Ser
Compositional biasi345 – 36420Poly-GlnAdd
BLAST
Compositional biasi448 – 4514Poly-His
Compositional biasi462 – 4654Poly-Ala
Compositional biasi471 – 4777Poly-Ala

Sequence similaritiesi

Contains 2 GATA-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri169 – 19325GATA-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri226 – 25025GATA-type 2PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1601. Eukaryota.
COG5641. LUCA.
GeneTreeiENSGT00760000119221.
InParanoidiP52168.
KOiK09183.
OMAiYESAMDF.
OrthoDBiEOG7CCBRF.
PhylomeDBiP52168.

Family and domain databases

Gene3Di3.30.50.10. 2 hits.
InterProiIPR028436. GATA_4/pnr.
IPR000679. Znf_GATA.
IPR013088. Znf_NHR/GATA.
[Graphical view]
PANTHERiPTHR10071:SF154. PTHR10071:SF154. 1 hit.
PfamiPF00320. GATA. 2 hits.
[Graphical view]
PRINTSiPR00619. GATAZNFINGER.
SMARTiSM00401. ZnF_GATA. 2 hits.
[Graphical view]
PROSITEiPS00344. GATA_ZN_FINGER_1. 2 hits.
PS50114. GATA_ZN_FINGER_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P52168-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGILLSDGDS TSDQQSTRDY PHFSGDYQNV TLSAASASTS ASASATHVAA
60 70 80 90 100
VKMYHSSAVA AYTDLAAAGS AASAGVGVGV SGYHQQAVNA PVYVPSNRQY
110 120 130 140 150
NHVAAHFGSA AAQNAWTTEG FGSAHAQFYS PNAAVMMGSW RSAYDPSGFQ
160 170 180 190 200
RSSPYESAMD FQFGEGRECV NCGAISTPLW RRDGTGHYLC NACGLYHKMN
210 220 230 240 250
GMNRPLIKPS KRLVSATATR RMGLCCTNCG TRTTTLWRRN NDGEPVCNAC
260 270 280 290 300
GLYYKLHGVN RPLAMRKDGI QTRKRKPKKT GSGSAVGAGT GSGTGSTLEA
310 320 330 340 350
IKECKEEHDL KPSLSLERHS LSKLHTDMKS GTSSSSTLMG HHSAQQQQQQ
360 370 380 390 400
QQQQQQQQQQ QQQQSAHQQC FPLYGQTTTQ QQHQQHGHSM TSSSGQAHLS
410 420 430 440 450
ARHLHGAAGT QLYTPGSSSG GGSASAYTSH SAETPALSNG TPSPHYQHHH
460 470 480 490 500
HLGGTHGHHV TAAAAHHHFH AAAAVAAYGV KTEASATNYD YVNNCYFGGT
510 520 530 540
FGALGGAATT TAMAGGAASE LAGYHHQHNV IQAAKLMATS
Length:540
Mass (Da):57,036
Last modified:October 1, 1996 - v1
Checksum:i46557C0754BEEB59
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti413 – 4131Y → S in AAB29876 (PubMed:7916679).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S68798
, S68909, S68793, S68795 Genomic DNA. Translation: AAB29874.1.
S68803, S68802 Genomic DNA. Translation: AAB29876.2.
AE014297 Genomic DNA. Translation: AAN13693.1.
RefSeqiNP_476685.1. NM_057337.3.
UniGeneiDm.7432.

Genome annotation databases

EnsemblMetazoaiFBtr0083221; FBpp0082675; FBgn0003117.
GeneIDi44849.
KEGGidme:Dmel_CG3978.
UCSCiCG3978-RA. d. melanogaster.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S68798
, S68909, S68793, S68795 Genomic DNA. Translation: AAB29874.1.
S68803, S68802 Genomic DNA. Translation: AAB29876.2.
AE014297 Genomic DNA. Translation: AAN13693.1.
RefSeqiNP_476685.1. NM_057337.3.
UniGeneiDm.7432.

3D structure databases

ProteinModelPortaliP52168.
SMRiP52168. Positions 165-276.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi69326. 66 interactions.
STRINGi7227.FBpp0082675.

Proteomic databases

PaxDbiP52168.
PRIDEiP52168.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0083221; FBpp0082675; FBgn0003117.
GeneIDi44849.
KEGGidme:Dmel_CG3978.
UCSCiCG3978-RA. d. melanogaster.

Organism-specific databases

CTDi44849.
FlyBaseiFBgn0003117. pnr.

Phylogenomic databases

eggNOGiKOG1601. Eukaryota.
COG5641. LUCA.
GeneTreeiENSGT00760000119221.
InParanoidiP52168.
KOiK09183.
OMAiYESAMDF.
OrthoDBiEOG7CCBRF.
PhylomeDBiP52168.

Enzyme and pathway databases

ReactomeiR-DME-5683826. Surfactant metabolism.

Miscellaneous databases

GenomeRNAii44849.
PROiP52168.

Gene expression databases

BgeeiP52168.
ExpressionAtlasiP52168. differential.
GenevisibleiP52168. DM.

Family and domain databases

Gene3Di3.30.50.10. 2 hits.
InterProiIPR028436. GATA_4/pnr.
IPR000679. Znf_GATA.
IPR013088. Znf_NHR/GATA.
[Graphical view]
PANTHERiPTHR10071:SF154. PTHR10071:SF154. 1 hit.
PfamiPF00320. GATA. 2 hits.
[Graphical view]
PRINTSiPR00619. GATAZNFINGER.
SMARTiSM00401. ZnF_GATA. 2 hits.
[Graphical view]
PROSITEiPS00344. GATA_ZN_FINGER_1. 2 hits.
PS50114. GATA_ZN_FINGER_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A GATA family transcription factor is expressed along the embryonic dorsoventral axis in Drosophila melanogaster."
    Winick J., Abel T., Leonard M.W., Michelson A.M., Chardon-Loriaux I., Holmgren R.A., Maniatis T., Engel J.D.
    Development 119:1055-1065(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Pannier, a negative regulator of achaete and scute in Drosophila, encodes a zinc finger protein with homology to the vertebrate transcription factor GATA-1."
    Ramain P., Heitzler P., Haenlin M., Simpson P.
    Development 119:1277-1291(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTANTS PNRD1; PNRD2; PNRD3 AND PNRD4.
  3. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  4. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  5. "Enhancer-promoter communication mediated by Chip during Pannier-driven proneural patterning is regulated by Osa."
    Heitzler P., Vanolst L., Biryukova I., Ramain P.
    Genes Dev. 17:591-596(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH OSA.

Entry informationi

Entry nameiPNR_DROME
AccessioniPrimary (citable) accession number: P52168
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 8, 2016
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.