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Protein

Presenilin sel-12

Gene

sel-12

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable catalytic subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors (lin-12 or glp-1). Provides the major presenilin function compared to hop-1 and spe-4. Required cell-autonomously for correct neurite connectivity of the AIY cholinergic interneurons and their correct functioning in thermotaxis. Required for mesodermal patterning of muscle function.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei226By similarity1
Active sitei364By similarity1

GO - Molecular functioni

GO - Biological processi

  • amyloid precursor protein catabolic process Source: GO_Central
  • apical protein localization Source: WormBase
  • beta-amyloid metabolic process Source: GO_Central
  • calcium ion transport Source: GO_Central
  • cell fate specification Source: WormBase
  • detection of temperature stimulus Source: UniProtKB
  • membrane protein ectodomain proteolysis Source: GO_Central
  • negative regulation of apoptotic process Source: GO_Central
  • nervous system development Source: UniProtKB
  • Notch receptor processing Source: GO_Central
  • Notch signaling pathway Source: UniProtKB
  • oviposition Source: WormBase
  • positive regulation of Notch signaling pathway Source: WormBase
  • post-embryonic animal organ morphogenesis Source: WormBase
  • protein processing Source: InterPro
  • regulation of transforming growth factor beta receptor signaling pathway Source: WormBase
Complete GO annotation...

Keywords - Biological processi

Notch signaling pathway

Enzyme and pathway databases

ReactomeiR-CEL-6798695. Neutrophil degranulation.
SignaLinkiP52166.

Protein family/group databases

MEROPSiA22.009.

Names & Taxonomyi

Protein namesi
Recommended name:
Presenilin sel-12
Alternative name(s):
Suppressor/enhancer of lin-12 protein 12
Gene namesi
Name:sel-12
ORF Names:F35H12.3
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome X

Organism-specific databases

WormBaseiF35H12.3; CE24946; WBGene00004769; sel-12.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 45CytoplasmicSequence analysisAdd BLAST45
Transmembranei46 – 66HelicalSequence analysisAdd BLAST21
Topological domaini67 – 101LumenalSequence analysisAdd BLAST35
Transmembranei102 – 122HelicalSequence analysisAdd BLAST21
Topological domaini123 – 130CytoplasmicSequence analysis8
Transmembranei131 – 151HelicalSequence analysisAdd BLAST21
Topological domaini152 – 163LumenalSequence analysisAdd BLAST12
Transmembranei164 – 184HelicalSequence analysisAdd BLAST21
Topological domaini185 – 189CytoplasmicSequence analysis5
Transmembranei190 – 210HelicalSequence analysisAdd BLAST21
Topological domaini211 – 212LumenalSequence analysis2
Transmembranei213 – 233HelicalSequence analysisAdd BLAST21
Topological domaini234 – 359CytoplasmicSequence analysisAdd BLAST126
Transmembranei360 – 380HelicalSequence analysisAdd BLAST21
Topological domaini381 – 384LumenalSequence analysis4
Transmembranei385 – 405HelicalSequence analysisAdd BLAST21
Topological domaini406 – 413CytoplasmicSequence analysis8
Intramembranei414 – 434HelicalSequence analysisAdd BLAST21
Topological domaini435 – 444CytoplasmicSequence analysis10

GO - Cellular componenti

  • apical plasma membrane Source: GO_Central
  • cell cortex Source: GO_Central
  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • gamma-secretase complex Source: WormBase
  • Golgi membrane Source: UniProtKB-SubCell
  • integral component of plasma membrane Source: GO_Central
  • neuronal cell body Source: GO_Central
  • nucleus Source: GO_Central
  • perinuclear region of cytoplasm Source: WormBase
  • Z disc Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi60C → S in ar131; egg-laying-defective. 2 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000739031 – 444Presenilin sel-12Add BLAST444

Proteomic databases

EPDiP52166.
PaxDbiP52166.

Expressioni

Tissue specificityi

Expressed in most neurons.1 Publication

Developmental stagei

Expressed both maternally and zygotically. Ubiquitously expressed throughout the development and in the adult.1 Publication

Gene expression databases

BgeeiWBGene00004769.

Interactioni

Subunit structurei

Homodimer. Component of the gamma-secretase complex, a complex probably composed of the presenilin homodimer (sel-12, hop-1 or spe-4), nicastrin (aph-2), aph-1 and pen-2 (Probable). Interacts with sel-10 (PubMed:9861048).Curated1 Publication

Protein-protein interaction databases

BioGridi45394. 56 interactors.
STRINGi6239.F35H12.3.

Structurei

3D structure databases

ProteinModelPortaliP52166.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi410 – 412PAL3

Domaini

The PAL motif is required for normal active site conformation.By similarity

Sequence similaritiesi

Belongs to the peptidase A22A family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2736. Eukaryota.
ENOG410XPZD. LUCA.
GeneTreeiENSGT00390000016593.
HOGENOMiHOG000240228.
InParanoidiP52166.
KOiK04505.
OMAiNAFERRT.
OrthoDBiEOG091G0C72.
PhylomeDBiP52166.

Family and domain databases

InterProiIPR001686. Pept_A22A_Ceel.
IPR001108. Peptidase_A22A.
IPR006639. Preselin/SPP.
[Graphical view]
PANTHERiPTHR10202. PTHR10202. 1 hit.
PfamiPF01080. Presenilin. 1 hit.
[Graphical view]
PRINTSiPR01072. PRESENILIN.
PR01075. PRESENILNSEL.
SMARTiSM00730. PSN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P52166-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPSTRRQQEG GGADAETHTV YGTNLITNRN SQEDENVVEE AELKYGASHV
60 70 80 90 100
IHLFVPVSLC MALVVFTMNT ITFYSQNNGR HLLYTPFVRE TDSIVEKGLM
110 120 130 140 150
SLGNALVMLC VVVLMTVLLI VFYKYKFYKL IHGWLIVSSF LLLFLFTTIY
160 170 180 190 200
VQEVLKSFDV SPSALLVLFG LGNYGVLGMM CIHWKGPLRL QQFYLITMSA
210 220 230 240 250
LMALVFIKYL PEWTVWFVLF VISVWDLVAV LTPKGPLRYL VETAQERNEP
260 270 280 290 300
IFPALIYSSG VIYPYVLVTA VENTTDPREP TSSDSNTSTA FPGEASCSSE
310 320 330 340 350
TPKRPKVKRI PQKVQIESNT TASTTQNSGV RVERELAAER PTVQDANFHR
360 370 380 390 400
HEEEERGVKL GLGDFIFYSV LLGKASSYFD WNTTIACYVA ILIGLCFTLV
410 420 430 440
LLAVFKRALP ALPISIFSGL IFYFCTRWII TPFVTQVSQK CLLY
Length:444
Mass (Da):50,034
Last modified:May 27, 2002 - v2
Checksum:i37ADBC124E16429C
GO

Sequence cautioni

The sequence AAA85511 differs from that shown. Reason: Frameshift at position 413.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U35660 mRNA. Translation: AAA85511.1. Frameshift.
AF171064 mRNA. Translation: AAD50991.1.
FO081301 Genomic DNA. Translation: CCD70617.1.
RefSeqiNP_508175.1. NM_075774.5.
UniGeneiCel.19557.

Genome annotation databases

EnsemblMetazoaiF35H12.3; F35H12.3; WBGene00004769.
GeneIDi180441.
KEGGicel:CELE_F35H12.3.
UCSCiF35H12.3. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U35660 mRNA. Translation: AAA85511.1. Frameshift.
AF171064 mRNA. Translation: AAD50991.1.
FO081301 Genomic DNA. Translation: CCD70617.1.
RefSeqiNP_508175.1. NM_075774.5.
UniGeneiCel.19557.

3D structure databases

ProteinModelPortaliP52166.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi45394. 56 interactors.
STRINGi6239.F35H12.3.

Protein family/group databases

MEROPSiA22.009.

Proteomic databases

EPDiP52166.
PaxDbiP52166.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiF35H12.3; F35H12.3; WBGene00004769.
GeneIDi180441.
KEGGicel:CELE_F35H12.3.
UCSCiF35H12.3. c. elegans.

Organism-specific databases

CTDi180441.
WormBaseiF35H12.3; CE24946; WBGene00004769; sel-12.

Phylogenomic databases

eggNOGiKOG2736. Eukaryota.
ENOG410XPZD. LUCA.
GeneTreeiENSGT00390000016593.
HOGENOMiHOG000240228.
InParanoidiP52166.
KOiK04505.
OMAiNAFERRT.
OrthoDBiEOG091G0C72.
PhylomeDBiP52166.

Enzyme and pathway databases

ReactomeiR-CEL-6798695. Neutrophil degranulation.
SignaLinkiP52166.

Miscellaneous databases

PROiP52166.

Gene expression databases

BgeeiWBGene00004769.

Family and domain databases

InterProiIPR001686. Pept_A22A_Ceel.
IPR001108. Peptidase_A22A.
IPR006639. Preselin/SPP.
[Graphical view]
PANTHERiPTHR10202. PTHR10202. 1 hit.
PfamiPF01080. Presenilin. 1 hit.
[Graphical view]
PRINTSiPR01072. PRESENILIN.
PR01075. PRESENILNSEL.
SMARTiSM00730. PSN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPSN_CAEEL
AccessioniPrimary (citable) accession number: P52166
Secondary accession number(s): Q20076, Q9U9C7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: May 27, 2002
Last modified: November 30, 2016
This is version 135 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.