P52137 (YFJV_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative arsenical pump membrane protein

Gene names

Name:	yfjV
Synonyms:	ypjM
Ordered Locus Names:	b2641
ORF Names:	b2639, b2640

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	338 AA.
Sequence status	Fragment.
Protein existence	Uncertain

General annotation (Comments)

Function	Involved in arsenical resistance. Thought to form the channel of an arsenite pump By similarity.
Subcellular location	Cell inner membrane; Multi-pass membrane protein Probable.
Sequence similarities	Belongs to the ArsB family.
Caution	This is a truncated ORF. Compared to E.coli W3110, the strain MG1655 has an apparent deletion of about 6 kb which seems to truncate this putative ArsB homolog. Could be the product of a pseudogene.
Sequence caution	The sequence AAA79807.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Arsenical resistance
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	arsenite transport Inferred from electronic annotation. Source: InterPro response to arsenic-containing substance Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	arsenite transmembrane transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – 338	›338	Putative arsenical pump membrane protein		PRO_0000201481
Regions
	Transmembrane	22 – 42	21	Helical; Potential
	Transmembrane	45 – 65	21	Helical; Potential
	Transmembrane	87 – 107	21	Helical; Potential
	Transmembrane	137 – 157	21	Helical; Potential
	Transmembrane	183 – 203	21	Helical; Potential
	Transmembrane	225 – 245	21	Helical; Potential
	Transmembrane	246 – 266	21	Helical; Potential
	Transmembrane	280 – 300	21	Helical; Potential
	Transmembrane	316 – 336	21	Helical; Potential
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	P52137 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: C0B2DF18AED8352A	FASTA	338	35,811
	10 20 30 40 50 60 RALFTWKVLL GAAVDALFAN DGAALILTPI VIAMLIALGF SQGTTLAFVM AAGFIADTAS 70 80 90 100 110 120 LPLIVSNLVN IVSADFFGLS FAQYASVMIS VDAAAIAATL IMLYLFFRRV IPATYXVSLL 130 140 150 160 170 180 KTPASAIKDL ATFRAGWIVL LLLLVGFFFL EPQGILVSAI AAAGAAVLFV VAKRGHSINT 190 200 210 220 230 240 GKVLRGAPWQ IVIFSLGMYI VVYGLRNAGF TEYLSGVLNL LADKGLWAAT FGTGFLTAFL 250 260 270 280 290 300 SSVMNNMPTV LIGALSIDGS TATGVVKEAM IYANVIGCDL GPKITPIGSL ATLLWLHVLA 310 320 330 QKNMTITWGY YFRTGIVMTV PVLFVTLAAL AWRLSVTL

References

[1]

"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U36840 Genomic DNA. Translation: AAA79807.1. Different initiation. U36840 Genomic DNA. Translation: AAA79808.1. Different termination. U36840 Genomic DNA. Translation: AAA79809.1. Frameshift. U00096 Genomic DNA. No translation available.
PIR	T08650. T08651. T08652.
3D structure databases
ProteinModelPortal	P52137.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-12087N.
IntAct	P52137. 1 interaction.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
EchoBASE	EB3000.
EcoGene	EG13208. yfjV.
Phylogenomic databases
HOGENOM	HOG000005271.
OMA	DVWFCAF.
Enzyme and pathway databases
BioCyc	EcoCyc:G7375-MONOMER.
Gene expression databases
Genevestigator	P52137.
Family and domain databases
InterPro	IPR000802. Arsenical_pump_ArsB. [Graphical view]
Pfam	PF02040. ArsB. 1 hit. [Graphical view]
PRINTS	PR00758. ARSENICPUMP.
TIGRFAMs	TIGR00935. 2a45. 1 hit.
ProtoNet	Search...

Entry information

Entry name

YFJV_ECOLI

Accession

Primary (citable) accession number: P52137
Secondary accession number(s): P76607, P76608, P76609

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 1, 2013
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program