Reviewed, UniProtKB/Swiss-Prot P52136 (YFJU_ECOLI)

Names and origin

Protein names

Recommended name:
    Putative arsenate reductase
    EC=1.20.4.1
Alternative name(s):
    Arsenical pump modifier

Gene names

Name:	yfjU
Ordered Locus Names:	b2638, JW2619

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	51 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Uncertain.

General annotation (Comments)

Function	Reduction of arsenate [As(V)] to arsenite [As(III)]. This protein expands the substrate specificity of arsAB pump which can extrude arsenite and antimonite to allow for arsenate pumping and resistance By similarity.
Catalytic activity	Arsenate + glutaredoxin = arsenite + glutaredoxin disulfide + H2O.
Sequence similarities	Belongs to the arsC family.
Caution	This is a truncated ORF. Compared to E.coli W3110, the strain MG1655 has an apparent deletion of about 6 kb which seems to truncate this putative arsC homolog. Could be the product of a pseudogene.

Ontologies

Keywords
Biological process	Arsenical resistance
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW response to arsenic Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	arsenate reductase (glutaredoxin) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›51	›51	Putative arsenate reductase		PRO_0000162582
Experimental info
	Non-terminal residue	51	1

Sequences

	Sequence		Length	Mass (Da)
	P52136-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: E3D33904ABB23560	FASTA	51	5,786
	10 20 30 40 50 MSNITIYHNP ACGTSRNTLE MLHNNGNEPT IINYLDMPPT RDELIKLISD I

References

[1]

"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.

[2]

"Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T. , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T. DNA Res. 4:91-113(1997) [PubMed: 9205837] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 1-48. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U36840 Genomic DNA. Translation: AAA79806.1. Different termination. U00096 Genomic DNA. No translation available. AP009048 Genomic DNA. Translation: BAA16506.1.
PIR	T08649.
RefSeq	AP_003217.1.
3D structure databases
HSSP	HSSP built from PDB template 1I9D based on UniProtKB P08692.
SMR	P52136. Positions 3-51.
ModBase	Search...
Genome annotation databases
GenomeReviews	Gene locus JW2619 in contig AP009048_GR. Gene locus b2638 in contig U00096_GR.
KEGG	ecj:JW2619. eco:b2638.
Organism-specific databases
EchoBASE	EB2999.
EcoGene	EG13207. yfjU.
CMR	Search...
Phylogenomic databases
HOGENOM	P52136.
Enzyme and pathway databases
BioCyc	EcoCyc:G7373-MON.
Family and domain databases
InterPro	IPR006660. Arsenate_reductase-like. IPR012335. Thioredoxin_fold. [Graphical view]
Gene3D	G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
Pfam	PF03960. ArsC. 1 hit. [Graphical view]
PROSITE	PS51353. ARSC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

YFJU_ECOLI

Accession

Primary (citable) accession number: P52136
Secondary accession number(s): P76606, P77012

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	March 3, 2009
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)