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Protein

Renin

Gene

REN

Organism
Ovis aries (Sheep)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney.

Catalytic activityi

Cleavage of Leu-|-Xaa bond in angiotensinogen to generate angiotensin I.

Enzyme regulationi

Interaction with ATP6AP2 results in a 5-fold increased efficiency in angiotensinogen processing.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei98 – 981PROSITE-ProRule annotation
Active sitei286 – 2861PROSITE-ProRule annotation

GO - Molecular functioni

  1. aspartic-type endopeptidase activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Aspartyl protease, Hydrolase, Protease

Protein family/group databases

MEROPSiA01.007.

Names & Taxonomyi

Protein namesi
Recommended name:
Renin (EC:3.4.23.15)
Alternative name(s):
Angiotensinogenase
Gene namesi
Name:REN
OrganismiOvis aries (Sheep)
Taxonomic identifieri9940 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeCaprinaeOvis
ProteomesiUP000002356: Unplaced

Subcellular locationi

Secreted By similarity. Membrane By similarity
Note: Associated to membranes via binding to ATP6AP2.By similarity

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
  2. membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1717Sequence AnalysisAdd
BLAST
Propeptidei18 – 5942Activation peptideSequence AnalysisPRO_0000026093Add
BLAST
Chaini60 – 400341ReninPRO_0000026094Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi65 – 651N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi111 ↔ 118By similarity
Glycosylationi135 – 1351N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi277 ↔ 281By similarity
Glycosylationi353 – 3531N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Expressioni

Tissue specificityi

Kidney.

Interactioni

Subunit structurei

Interacts with ATP6AP2.By similarity

Structurei

3D structure databases

ProteinModelPortaliP52115.
SMRiP52115. Positions 68-400.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase A1 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

HOVERGENiHBG000482.
KOiK01380.

Family and domain databases

Gene3Di2.40.70.10. 2 hits.
InterProiIPR001461. Aspartic_peptidase.
IPR001969. Aspartic_peptidase_AS.
IPR012848. Aspartic_peptidase_N.
IPR021109. Peptidase_aspartic_dom.
[Graphical view]
PANTHERiPTHR13683. PTHR13683. 1 hit.
PfamiPF07966. A1_Propeptide. 1 hit.
PF00026. Asp. 1 hit.
[Graphical view]
PRINTSiPR00792. PEPSIN.
SUPFAMiSSF50630. SSF50630. 1 hit.
PROSITEiPS00141. ASP_PROTEASE. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P52115-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPLWGLLLAL WGCSTFSLPA DTAAFRRIFL KKMPSVRESL KERGVDMAQL
60 70 80 90 100
GAEWSQLTKT LSFGNRTSPV VLTNYLDTQY YGEIGIGTPP QTFKVIFDTG
110 120 130 140 150
SANLWVPSTK CSPLYTACEI HSLYDSLESS SYVENGTEFT IYYGSGKVKG
160 170 180 190 200
FLSQDLVTVG GITVTQTFGE VTELPLRPFM LAKFDGVLGM GFPAQAVGGV
210 220 230 240 250
TPVFDHILAQ RVLTEDVFSV YYSRDSKNSH LLGGEIVLGG SDPQYYQENF
260 270 280 290 300
HYVSISKPGS WQIRMKGVSV RSTTLLCEEG CMVVVDTGAS YISGPTSSLR
310 320 330 340 350
LLMEALGAKE LSIDEYVVNC NQMPTLPDIS FHLGGKAYTL TSADYVLQDP
360 370 380 390 400
YNNISCTLAL HGMDIPPPTG PVWVLGATFI RKFYTEFDRR NNRIGFALAR
Length:400
Mass (Da):44,016
Last modified:October 1, 1996 - v1
Checksum:i95ED40716565FE25
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L43524 mRNA. Translation: AAA69809.1.
PIRiI47099.
RefSeqiNP_001009299.1. NM_001009299.1.
UniGeneiOar.403.

Genome annotation databases

GeneIDi443310.
KEGGioas:443310.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L43524 mRNA. Translation: AAA69809.1.
PIRiI47099.
RefSeqiNP_001009299.1. NM_001009299.1.
UniGeneiOar.403.

3D structure databases

ProteinModelPortaliP52115.
SMRiP52115. Positions 68-400.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiA01.007.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi443310.
KEGGioas:443310.

Organism-specific databases

CTDi5972.

Phylogenomic databases

HOVERGENiHBG000482.
KOiK01380.

Family and domain databases

Gene3Di2.40.70.10. 2 hits.
InterProiIPR001461. Aspartic_peptidase.
IPR001969. Aspartic_peptidase_AS.
IPR012848. Aspartic_peptidase_N.
IPR021109. Peptidase_aspartic_dom.
[Graphical view]
PANTHERiPTHR13683. PTHR13683. 1 hit.
PfamiPF07966. A1_Propeptide. 1 hit.
PF00026. Asp. 1 hit.
[Graphical view]
PRINTSiPR00792. PEPSIN.
SUPFAMiSSF50630. SSF50630. 1 hit.
PROSITEiPS00141. ASP_PROTEASE. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The sequence and tissue expression of ovine renin."
    Aldred G.P., Fu P., Crawford R.J., Fernley R.T.
    J. Mol. Endocrinol. 8:3-11(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Merino.
    Tissue: Kidney.

Entry informationi

Entry nameiRENI_SHEEP
AccessioniPrimary (citable) accession number: P52115
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: January 7, 2015
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.