Reviewed, UniProtKB/Swiss-Prot P52115 (RENI_SHEEP)

Names and origin

Protein names

Recommended name:
    Renin
    EC=3.4.23.15
Alternative name(s):
    Angiotensinogenase

Gene names

Name:

REN

Organism

Ovis aries (Sheep)

Taxonomic identifier

9940 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Caprinae › Ovis

Protein attributes

Sequence length	400 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney.
Catalytic activity	Cleavage of Leu-|-Xaa bond in angiotensinogen to generate angiotensin I.
Enzyme regulation	Interaction with ATP6AP2 results in a 5-fold increased efficiency in angiotensinogen processing By similarity.
Subunit structure	Interacts with ATP6AP2 By similarity.
Subcellular location	SecretedBy similarity. MembraneBy similarity. Note= Associated to membranes via binding to ATP6AP2 By similarity.
Tissue specificity	Kidney.
Sequence similarities	Belongs to the peptidase A1 family.

Ontologies

Keywords
Cellular component	Membrane Secreted
Domain	Signal
Molecular function	Aspartyl protease Hydrolase Protease
PTM	Glycoprotein Zymogen
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-KW membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	aspartic-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 17	17	Potential
	Propeptide	18 – 59	42	Activation peptide Potential		PRO_0000026093
	Chain	60 – 400	341	Renin		PRO_0000026094
Sites
	Active site	98	1	By similarity
	Active site	286	1	By similarity
Amino acid modifications
	Glycosylation	65	1	N-linked (GlcNAc...) Potential
	Glycosylation	135	1	N-linked (GlcNAc...) Potential
	Glycosylation	353	1	N-linked (GlcNAc...) Potential
	Disulfide bond	111 ↔ 118		By similarity
	Disulfide bond	277 ↔ 281		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P52115-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 95ED40716565FE25	FASTA	400	44,016
	10 20 30 40 50 60 MPLWGLLLAL WGCSTFSLPA DTAAFRRIFL KKMPSVRESL KERGVDMAQL GAEWSQLTKT 70 80 90 100 110 120 LSFGNRTSPV VLTNYLDTQY YGEIGIGTPP QTFKVIFDTG SANLWVPSTK CSPLYTACEI 130 140 150 160 170 180 HSLYDSLESS SYVENGTEFT IYYGSGKVKG FLSQDLVTVG GITVTQTFGE VTELPLRPFM 190 200 210 220 230 240 LAKFDGVLGM GFPAQAVGGV TPVFDHILAQ RVLTEDVFSV YYSRDSKNSH LLGGEIVLGG 250 260 270 280 290 300 SDPQYYQENF HYVSISKPGS WQIRMKGVSV RSTTLLCEEG CMVVVDTGAS YISGPTSSLR 310 320 330 340 350 360 LLMEALGAKE LSIDEYVVNC NQMPTLPDIS FHLGGKAYTL TSADYVLQDP YNNISCTLAL 370 380 390 400 HGMDIPPPTG PVWVLGATFI RKFYTEFDRR NNRIGFALAR

References

[1]	"The sequence and tissue expression of ovine renin." Aldred G.P., Fu P., Crawford R.J., Fernley R.T. J. Mol. Endocrinol. 8:3-11(1992) [PubMed: 1543532] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Merino. Tissue: Kidney.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L43524 mRNA. Translation: AAA69809.1.
PIR	I47099.
RefSeq	NP_001009299.1.
UniGene	Oar.403
3D structure databases
HSSP	HSSP built from PDB template 1SMR based on UniProtKB P00796.
SMR	P52115. Positions 63-400.
ModBase	Search...
Protein family/group databases
MEROPS	A01.007.
Genome annotation databases
GeneID	443310.
Phylogenomic databases
HOVERGEN	P52115.
Family and domain databases
InterPro	IPR001969. Pept_Asp_AS. IPR009007. Pept_Aspartc_cat. IPR001461. Peptidase_A1. IPR012848. Propep_A1. [Graphical view]
Gene3D	G3DSA:2.40.70.10. Pept_Aspartc_cat. 1 hit.
PANTHER	PTHR13683. Peptidase_A1. 1 hit.
Pfam	PF07966. A1_Propeptide. 1 hit. PF00026. Asp. 1 hit. [Graphical view]
PRINTS	PR00792. PEPSIN.
PROSITE	PS00141. ASP_PROTEASE. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RENI_SHEEP

Accession

Primary (citable) accession number: P52115

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	November 25, 2008
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)