Transcriptional regulatory protein RstA - Escherichia coli (strain K12)

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P52108 (RSTA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 107. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Transcriptional regulatory protein RstA

Gene names

Name:	rstA
Synonyms:	urpT
Ordered Locus Names:	b1608, JW1600

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length	239 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Member of the two-component regulatory system RstB/RstA.
Subcellular location	Cytoplasm Potential.
Induction	Induced by low extracellular levels of magnesium via the PhoQ/PhoP two-component regulatory system. Ref.6
Post-translational modification	Phosphorylated by RstB. Ref.7
Sequence similarities	Contains 1 response regulatory domain.
Sequence caution	The sequence AAA82081.1 differs from that shown. Reason: Erroneous initiation. The sequence BAA15346.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Transcription Transcription regulation Two-component regulatory system
Cellular component	Cytoplasm
Ligand	DNA-binding
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	intracellular signal transduction Inferred from electronic annotation. Source: GOC regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW phosphorelay response regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
kdpE	P21866	2	EBI-558990,EBI-6403634

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 239

239

Transcriptional regulatory protein RstA

PRO_0000081223

Regions

Domain

3 – 116

114

Response regulatory

Amino acid modifications

Modified residue

4-aspartylphosphate By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

P52108 [UniParc].

Last modified June 26, 2007. Version 2.
Checksum: 409A1B3A2588A77D
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FASTA

239

26,704

        10         20         30         40         50         60 
MNTIVFVEDD AEVGSLIAAY LAKHDMQVTV EPRGDQAEET ILRENPDLVL LDIMLPGKDG 

        70         80         90        100        110        120 
MTICRDLRAK WSGPIVLLTS LDSDMNHILA LEMGACDYIL KTTPPAVLLA RLRLHLRQNE 

       130        140        150        160        170        180 
QATLTKGLQE TSLTPYKALH FGTLTIDPIN RVVTLANTEI SLSTADFELL WELATHAGQI 

       190        200        210        220        230 
MDRDALLKNL RGVSYDGLDR SVDVAISRLR KKLLDNAAEP YRIKTVRNKG YLFAPHAWE

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Kuempel P.L. Submitted (NOV-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T., Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K. Horiuchi T. DNA Res. 3:363-377(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"In vivo characterization of tus gene expression in Escherichia coli." Roecklein B.A., Kuempel P.L. Mol. Microbiol. 6:1655-1661(1992) [PubMed] [Europe PMC] [Abstract] Cited for: MAPPING.
[6]	"Identification and molecular characterization of the Mg2+ stimulon of Escherichia coli." Minagawa S., Ogasawara H., Kato A., Yamamoto K., Eguchi Y., Oshima T., Mori H., Ishihama A., Utsumi R. J. Bacteriol. 185:3696-3702(2003) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION. Strain: K12.
[7]	"Functional characterization in vitro of all two-component signal transduction systems from Escherichia coli." Yamamoto K., Hirao K., Oshima T., Aiba H., Utsumi R., Ishihama A. J. Biol. Chem. 280:1448-1456(2005) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U41101 Genomic DNA. Translation: AAA82081.1. Different initiation. U00096 Genomic DNA. Translation: AAC74680.2. AP009048 Genomic DNA. Translation: BAA15346.1. Different initiation.
PIR	B64917.
RefSeq	NP_416125.2. NC_000913.2. YP_489871.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P52108.
SMR	P52108. Positions 4-235.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-10807N.
IntAct	P52108. 12 interactions.
MINT	MINT-1274913.
STRING	511145.b1608.
Proteomic databases
PaxDb	P52108.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC74680; AAC74680; b1608. BAA15346; BAA15346; BAA15346.
GeneID	12932769. 946199.
KEGG	ecj:Y75_p1584. eco:b1608.
PATRIC	32118518. VBIEscCol129921_1679.
Organism-specific databases
EchoBASE	EB2982.
EcoGene	EG13190. rstA.
Phylogenomic databases
eggNOG	COG0745.
HOGENOM	HOG000034819.
KO	K07661.
OMA	HKSLHFG.
ProtClustDB	PRK10701.
Enzyme and pathway databases
BioCyc	EcoCyc:RSTA-MONOMER. ECOL316407:JW1600-MONOMER.
Gene expression databases
Genevestigator	P52108.
Family and domain databases
Gene3D	1.10.10.10. 1 hit.
InterPro	IPR011006. CheY-like_superfamily. IPR001867. Sig_transdc_resp-reg_C. IPR016032. Sig_transdc_resp-reg_C-effctor. IPR001789. Sig_transdc_resp-reg_receiver. IPR011991. WHTH_DNA-bd_dom. [Graphical view]
Pfam	PF00072. Response_reg. 1 hit. PF00486. Trans_reg_C. 1 hit. [Graphical view]
SMART	SM00448. REC. 1 hit. SM00862. Trans_reg_C. 1 hit. [Graphical view]
SUPFAM	SSF46894. Bipartite_resp_reg_C-effector. 1 hit. SSF52172. CheY_like. 1 hit.
PROSITE	PS50110. RESPONSE_REGULATORY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RSTA_ECOLI

Accession

Primary (citable) accession number: P52108

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	June 26, 2007
Last modified:	May 1, 2013
This is version 107 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents