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Protein

Transcriptional regulatory protein RstA

Gene

rstA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Member of the two-component regulatory system RstB/RstA.

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW

GO - Biological processi

  1. phosphorelay signal transduction system Source: UniProtKB-KW
  2. regulation of transcription, DNA-templated Source: UniProtKB-KW
  3. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation, Two-component regulatory system

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:RSTA-MONOMER.
ECOL316407:JW1600-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcriptional regulatory protein RstA
Gene namesi
Name:rstA
Synonyms:urpT
Ordered Locus Names:b1608, JW1600
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG13190. rstA.

Subcellular locationi

Cytoplasm Curated

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 239239Transcriptional regulatory protein RstAPRO_0000081223Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei52 – 5214-aspartylphosphatePROSITE-ProRule annotation

Post-translational modificationi

Phosphorylated by RstB.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP52108.

Expressioni

Inductioni

Induced by low extracellular levels of magnesium via the PhoQ/PhoP two-component regulatory system.1 Publication

Gene expression databases

GenevestigatoriP52108.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
kdpEP218662EBI-558990,EBI-6403634

Protein-protein interaction databases

DIPiDIP-10807N.
IntActiP52108. 12 interactions.
MINTiMINT-1274913.
STRINGi511145.b1608.

Structurei

3D structure databases

ProteinModelPortaliP52108.
SMRiP52108. Positions 2-107, 132-239.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 116114Response regulatoryPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 response regulatory domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0745.
HOGENOMiHOG000034819.
InParanoidiP52108.
KOiK07661.
OMAiLAMLTQD.
OrthoDBiEOG6G4VQG.
PhylomeDBiP52108.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR011006. CheY-like_superfamily.
IPR001867. Sig_transdc_resp-reg_C.
IPR016032. Sig_transdc_resp-reg_C-effctor.
IPR001789. Sig_transdc_resp-reg_receiver.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00072. Response_reg. 1 hit.
PF00486. Trans_reg_C. 1 hit.
[Graphical view]
SMARTiSM00448. REC. 1 hit.
SM00862. Trans_reg_C. 1 hit.
[Graphical view]
SUPFAMiSSF46894. SSF46894. 1 hit.
SSF52172. SSF52172. 1 hit.
PROSITEiPS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P52108-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNTIVFVEDD AEVGSLIAAY LAKHDMQVTV EPRGDQAEET ILRENPDLVL
60 70 80 90 100
LDIMLPGKDG MTICRDLRAK WSGPIVLLTS LDSDMNHILA LEMGACDYIL
110 120 130 140 150
KTTPPAVLLA RLRLHLRQNE QATLTKGLQE TSLTPYKALH FGTLTIDPIN
160 170 180 190 200
RVVTLANTEI SLSTADFELL WELATHAGQI MDRDALLKNL RGVSYDGLDR
210 220 230
SVDVAISRLR KKLLDNAAEP YRIKTVRNKG YLFAPHAWE
Length:239
Mass (Da):26,704
Last modified:June 26, 2007 - v2
Checksum:i409A1B3A2588A77D
GO

Sequence cautioni

The sequence AAA82081.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence BAA15346.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U41101 Genomic DNA. Translation: AAA82081.1. Different initiation.
U00096 Genomic DNA. Translation: AAC74680.2.
AP009048 Genomic DNA. Translation: BAA15346.1. Different initiation.
PIRiB64917.
RefSeqiNP_416125.2. NC_000913.3.
YP_489871.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC74680; AAC74680; b1608.
BAA15346; BAA15346; BAA15346.
GeneIDi12932769.
946199.
KEGGiecj:Y75_p1584.
eco:b1608.
PATRICi32118518. VBIEscCol129921_1679.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U41101 Genomic DNA. Translation: AAA82081.1. Different initiation.
U00096 Genomic DNA. Translation: AAC74680.2.
AP009048 Genomic DNA. Translation: BAA15346.1. Different initiation.
PIRiB64917.
RefSeqiNP_416125.2. NC_000913.3.
YP_489871.1. NC_007779.1.

3D structure databases

ProteinModelPortaliP52108.
SMRiP52108. Positions 2-107, 132-239.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-10807N.
IntActiP52108. 12 interactions.
MINTiMINT-1274913.
STRINGi511145.b1608.

Proteomic databases

PaxDbiP52108.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74680; AAC74680; b1608.
BAA15346; BAA15346; BAA15346.
GeneIDi12932769.
946199.
KEGGiecj:Y75_p1584.
eco:b1608.
PATRICi32118518. VBIEscCol129921_1679.

Organism-specific databases

EchoBASEiEB2982.
EcoGeneiEG13190. rstA.

Phylogenomic databases

eggNOGiCOG0745.
HOGENOMiHOG000034819.
InParanoidiP52108.
KOiK07661.
OMAiLAMLTQD.
OrthoDBiEOG6G4VQG.
PhylomeDBiP52108.

Enzyme and pathway databases

BioCyciEcoCyc:RSTA-MONOMER.
ECOL316407:JW1600-MONOMER.

Miscellaneous databases

PROiP52108.

Gene expression databases

GenevestigatoriP52108.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR011006. CheY-like_superfamily.
IPR001867. Sig_transdc_resp-reg_C.
IPR016032. Sig_transdc_resp-reg_C-effctor.
IPR001789. Sig_transdc_resp-reg_receiver.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00072. Response_reg. 1 hit.
PF00486. Trans_reg_C. 1 hit.
[Graphical view]
SMARTiSM00448. REC. 1 hit.
SM00862. Trans_reg_C. 1 hit.
[Graphical view]
SUPFAMiSSF46894. SSF46894. 1 hit.
SSF52172. SSF52172. 1 hit.
PROSITEiPS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Kuempel P.L.
    Submitted (NOV-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "In vivo characterization of tus gene expression in Escherichia coli."
    Roecklein B.A., Kuempel P.L.
    Mol. Microbiol. 6:1655-1661(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: MAPPING.
  6. "Identification and molecular characterization of the Mg2+ stimulon of Escherichia coli."
    Minagawa S., Ogasawara H., Kato A., Yamamoto K., Eguchi Y., Oshima T., Mori H., Ishihama A., Utsumi R.
    J. Bacteriol. 185:3696-3702(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
    Strain: K12.
  7. "Functional characterization in vitro of all two-component signal transduction systems from Escherichia coli."
    Yamamoto K., Hirao K., Oshima T., Aiba H., Utsumi R., Ishihama A.
    J. Biol. Chem. 280:1448-1456(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION.
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Entry informationi

Entry nameiRSTA_ECOLI
AccessioniPrimary (citable) accession number: P52108
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: June 26, 2007
Last modified: January 7, 2015
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.