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Protein

CsgBAC operon transcriptional regulatory protein

Gene

csgD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The master regulator for adhesive curli fimbriae expression; necessary for transcription of the csgBAC/ymdA operon. Plays a positive role in biofilm formation. May have the capability to respond to starvation and/or high cell density by activating csgBA transcription. Low-level constitutive expression confers an adherent curli fimbriae-expressing phenotype, up-regulates 10 genes and down-regulates 14 others.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi173 – 192H-T-H motifPROSITE-ProRule annotationAdd BLAST20

GO - Molecular functioni

GO - Biological processi

  • positive regulation of DNA-templated transcription, initiation Source: EcoCyc
  • regulation of single-species biofilm formation Source: EcoCyc
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionDNA-binding
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

BioCyciEcoCyc:PD01379

Names & Taxonomyi

Protein namesi
Recommended name:
CsgBAC operon transcriptional regulatory protein
Gene namesi
Name:csgD
Ordered Locus Names:b1040, JW1023
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13410 csgD

Subcellular locationi

  • Cell inner membrane 1 Publication; Peripheral membrane protein 1 Publication
  • Note: In experiments done with low-level constitutively expressed protein.

GO - Cellular componenti

  • plasma membrane Source: UniProtKB-SubCell
  • protein-DNA complex Source: CollecTF

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

No curli production.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001841431 – 216CsgBAC operon transcriptional regulatory proteinAdd BLAST216

Proteomic databases

PaxDbiP52106
PRIDEiP52106

Expressioni

Inductioni

Regulation is very complex. Strongly induced at 28 degrees Celsius (at protein level), transcription regulation requires c-di-GMP, although the mechanism is currently unknown. c-di-GMP levels are stimulated by the diguanylate cyclase DgcM and repressed by the c-di-GMP phosphodiesterase PdeR (YciR) (PubMed:17010156). Transcription directly repressed by MqsA, and indirectly repressed by MqsA via MqsA's repression of rpoS (PubMed:24212724).2 Publications

Gene expression databases

CollecTFiEXPREG_00000b00

Interactioni

Protein-protein interaction databases

BioGridi4260064, 9 interactors
DIPiDIP-9328N
IntActiP52106, 2 interactors
STRINGi316385.ECDH10B_1112

Structurei

3D structure databases

ProteinModelPortaliP52106
SMRiP52106
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini149 – 214HTH luxR-typePROSITE-ProRule annotationAdd BLAST66

Phylogenomic databases

eggNOGiENOG41086KC Bacteria
COG2771 LUCA
HOGENOMiHOG000284130
KOiK04333
OMAiTHLYRAF
PhylomeDBiP52106

Family and domain databases

CDDicd06170 LuxR_C_like, 1 hit
Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR016032 Sig_transdc_resp-reg_C-effctor
IPR000792 Tscrpt_reg_LuxR_C
IPR036388 WH-like_DNA-bd_sf
PfamiView protein in Pfam
PF00196 GerE, 1 hit
PRINTSiPR00038 HTHLUXR
SMARTiView protein in SMART
SM00421 HTH_LUXR, 1 hit
SUPFAMiSSF46894 SSF46894, 1 hit
PROSITEiView protein in PROSITE
PS00622 HTH_LUXR_1, 1 hit
PS50043 HTH_LUXR_2, 1 hit

Sequencei

Sequence statusi: Complete.

P52106-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFNEVHSIHG HTLLLITKSS LQATALLQHL KQSLAITGKL HNIQRSLDDI
60 70 80 90 100
SSGSIILLDM MEADKKLIHY WQDTLSRKNN NIKILLLNTP EDYPYRDIEN
110 120 130 140 150
WPHINGVFYS MEDQERVVNG LQGVLRGECY FTQKLASYLI THSGNYRYNS
160 170 180 190 200
TESALLTHRE KEILNKLRIG ASNNEIARSL FISENTVKTH LYNLFKKIAV
210
KNRTQAVSWA NDNLRR
Length:216
Mass (Da):24,935
Last modified:October 1, 1996 - v1
Checksum:iE1A5FD0DA1855DDE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X90754 Genomic DNA Translation: CAA62280.1
U00096 Genomic DNA Translation: AAC74124.1
AP009048 Genomic DNA Translation: BAA35830.1
AF081826 Genomic DNA Translation: AAD16025.1
PIRiS70786
RefSeqiNP_415558.1, NC_000913.3
WP_000481509.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74124; AAC74124; b1040
BAA35830; BAA35830; BAA35830
GeneIDi949119
KEGGiecj:JW1023
eco:b1040
PATRICifig|1411691.4.peg.1231

Similar proteinsi

Entry informationi

Entry nameiCSGD_ECOLI
AccessioniPrimary (citable) accession number: P52106
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: March 28, 2018
This is version 132 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health