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Protein

CsgBAC operon transcriptional regulatory protein

Gene

csgD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

The master regulator for adhesive curli fimbriae expression; necessary for transcription of the csgBAC/ymdA operon. Plays a positive role in biofilm formation. May have the capability to respond to starvation and/or high cell density by activating csgBA transcription. Low-level constitutive expression confers an adherent curli fimbriae-expressing phenotype, up-regulates 10 genes and down-regulates 14 others.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi173 – 19220H-T-H motifPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • positive regulation of DNA-templated transcription, initiation Source: EcoCyc
  • regulation of single-species biofilm formation Source: EcoCyc
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:PD01379.
ECOL316407:JW1023-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
CsgBAC operon transcriptional regulatory protein
Gene namesi
Name:csgD
Ordered Locus Names:b1040, JW1023
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13410. csgD.

Subcellular locationi

  • Cell inner membrane 1 Publication; Peripheral membrane protein 1 Publication

  • Note: In experiments done with low-level constitutively expressed protein.

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

No curli production.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 216216CsgBAC operon transcriptional regulatory proteinPRO_0000184143Add
BLAST

Proteomic databases

PaxDbiP52106.
PRIDEiP52106.

Expressioni

Inductioni

Regulation is very complex. Strongly induced at 28 degrees Celsius (at protein level), transcription regulation requires c-di-GMP, although the mechanism is currently unknown. c-di-GMP levels are stimulated by the diguanylate cyclase YdaM and repressed by the c-di-GMP phosphodiesterase Gmr (YciR) (PubMed:17010156). Transcription directly repressed by MqsA, and indirectly repressed by MqsA via MqsA's repression of rpoS (PubMed:24212724).2 Publications

Gene expression databases

CollecTFiEXPREG_00000b00.

Interactioni

Protein-protein interaction databases

BioGridi4260064. 9 interactions.
DIPiDIP-9328N.
IntActiP52106. 2 interactions.
MINTiMINT-1293972.
STRINGi511145.b1040.

Structurei

3D structure databases

ProteinModelPortaliP52106.
SMRiP52106. Positions 21-214.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini149 – 21466HTH luxR-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 HTH luxR-type DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG41086KC. Bacteria.
COG2771. LUCA.
HOGENOMiHOG000284130.
KOiK04333.
OMAiRKNNNIR.
PhylomeDBiP52106.

Family and domain databases

CDDicd06170. LuxR_C_like. 1 hit.
Gene3Di1.10.10.10. 1 hit.
InterProiIPR016032. Sig_transdc_resp-reg_C-effctor.
IPR000792. Tscrpt_reg_LuxR_C.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00196. GerE. 1 hit.
[Graphical view]
PRINTSiPR00038. HTHLUXR.
SMARTiSM00421. HTH_LUXR. 1 hit.
[Graphical view]
SUPFAMiSSF46894. SSF46894. 1 hit.
PROSITEiPS00622. HTH_LUXR_1. 1 hit.
PS50043. HTH_LUXR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P52106-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFNEVHSIHG HTLLLITKSS LQATALLQHL KQSLAITGKL HNIQRSLDDI
60 70 80 90 100
SSGSIILLDM MEADKKLIHY WQDTLSRKNN NIKILLLNTP EDYPYRDIEN
110 120 130 140 150
WPHINGVFYS MEDQERVVNG LQGVLRGECY FTQKLASYLI THSGNYRYNS
160 170 180 190 200
TESALLTHRE KEILNKLRIG ASNNEIARSL FISENTVKTH LYNLFKKIAV
210
KNRTQAVSWA NDNLRR
Length:216
Mass (Da):24,935
Last modified:October 1, 1996 - v1
Checksum:iE1A5FD0DA1855DDE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X90754 Genomic DNA. Translation: CAA62280.1.
U00096 Genomic DNA. Translation: AAC74124.1.
AP009048 Genomic DNA. Translation: BAA35830.1.
AF081826 Genomic DNA. Translation: AAD16025.1.
PIRiS70786.
RefSeqiNP_415558.1. NC_000913.3.
WP_000481509.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74124; AAC74124; b1040.
BAA35830; BAA35830; BAA35830.
GeneIDi949119.
KEGGiecj:JW1023.
eco:b1040.
PATRICi32117313. VBIEscCol129921_1079.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X90754 Genomic DNA. Translation: CAA62280.1.
U00096 Genomic DNA. Translation: AAC74124.1.
AP009048 Genomic DNA. Translation: BAA35830.1.
AF081826 Genomic DNA. Translation: AAD16025.1.
PIRiS70786.
RefSeqiNP_415558.1. NC_000913.3.
WP_000481509.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP52106.
SMRiP52106. Positions 21-214.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260064. 9 interactions.
DIPiDIP-9328N.
IntActiP52106. 2 interactions.
MINTiMINT-1293972.
STRINGi511145.b1040.

Proteomic databases

PaxDbiP52106.
PRIDEiP52106.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74124; AAC74124; b1040.
BAA35830; BAA35830; BAA35830.
GeneIDi949119.
KEGGiecj:JW1023.
eco:b1040.
PATRICi32117313. VBIEscCol129921_1079.

Organism-specific databases

EchoBASEiEB3186.
EcoGeneiEG13410. csgD.

Phylogenomic databases

eggNOGiENOG41086KC. Bacteria.
COG2771. LUCA.
HOGENOMiHOG000284130.
KOiK04333.
OMAiRKNNNIR.
PhylomeDBiP52106.

Enzyme and pathway databases

BioCyciEcoCyc:PD01379.
ECOL316407:JW1023-MONOMER.

Miscellaneous databases

PROiP52106.

Gene expression databases

CollecTFiEXPREG_00000b00.

Family and domain databases

CDDicd06170. LuxR_C_like. 1 hit.
Gene3Di1.10.10.10. 1 hit.
InterProiIPR016032. Sig_transdc_resp-reg_C-effctor.
IPR000792. Tscrpt_reg_LuxR_C.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00196. GerE. 1 hit.
[Graphical view]
PRINTSiPR00038. HTHLUXR.
SMARTiSM00421. HTH_LUXR. 1 hit.
[Graphical view]
SUPFAMiSSF46894. SSF46894. 1 hit.
PROSITEiPS00622. HTH_LUXR_1. 1 hit.
PS50043. HTH_LUXR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCSGD_ECOLI
AccessioniPrimary (citable) accession number: P52106
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: September 7, 2016
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.