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Protein

Sensor histidine kinase GlrK

Gene

glrK

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Member of the two-component regulatory system GlrR/GlrK that up-regulates transcription of the glmY sRNA when cells enter the stationary growth phase. Activates GlrR by phosphorylation.2 Publications

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • phosphorelay sensor kinase activity Source: EcoCyc

GO - Biological processi

  • phosphorelay signal transduction system Source: EcoCyc
  • protein autophosphorylation Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Two-component regulatory system

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:G7345-MONOMER.
ECOL316407:JW5407-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Sensor histidine kinase GlrK (EC:2.7.13.3)
Gene namesi
Name:glrK
Synonyms:yfhK
Ordered Locus Names:b2556, JW5407
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13461. glrK.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 13CytoplasmicSequence analysisAdd BLAST13
Transmembranei14 – 34HelicalSequence analysisAdd BLAST21
Topological domaini35 – 173PeriplasmicSequence analysisAdd BLAST139
Transmembranei174 – 194HelicalSequence analysisAdd BLAST21
Topological domaini195 – 475CytoplasmicSequence analysisAdd BLAST281

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • intracellular Source: GOC
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Mutants show decreased amounts of glmY.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000749151 – 475Sensor histidine kinase GlrKAdd BLAST475

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei259Phosphohistidine; by autocatalysisPROSITE-ProRule annotation1

Post-translational modificationi

Autophosphorylated.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP52101.
PRIDEiP52101.

Interactioni

Protein-protein interaction databases

DIPiDIP-12052N.
STRINGi511145.b2556.

Structurei

3D structure databases

ProteinModelPortaliP52101.
SMRiP52101.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini256 – 472Histidine kinasePROSITE-ProRule annotationAdd BLAST217

Sequence similaritiesi

Contains 1 histidine kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4107WP9. Bacteria.
ENOG410Y4CW. LUCA.
HOGENOMiHOG000284457.
InParanoidiP52101.
KOiK07711.
OMAiINFSHET.
PhylomeDBiP52101.

Family and domain databases

Gene3Di1.10.287.130. 1 hit.
3.30.565.10. 1 hit.
InterProiIPR003660. HAMP_dom.
IPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR004358. Sig_transdc_His_kin-like_C.
[Graphical view]
PfamiPF00672. HAMP. 1 hit.
PF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
[Graphical view]
SUPFAMiSSF47384. SSF47384. 1 hit.
SSF55874. SSF55874. 1 hit.
PROSITEiPS50109. HIS_KIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P52101-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRWPVFPRS LRQLVMLAFL LILLPLLVLA WQAWQSLNAL SDQAALVNRT
60 70 80 90 100
TLIDARRSEA MTNAALEMER SYRQYCVLDD PTLAKVYQSQ RKRYSEMLDA
110 120 130 140 150
HAGVLPDDKL YQALRQDLHN LAQLQCNNSG PDAAAAARLE AFASANTEMV
160 170 180 190 200
QATRTVVFSR GQQLQREIAE RGQYFGWQSL VLFLVSLVMV LLFTRMIIGP
210 220 230 240 250
VKNIERMINR LGEGRSLGNS VSFSGPSELR SVGQRILWLS ERLSWLESQR
260 270 280 290 300
HQFLRHLSHE LKTPLASMRE GTELLADQVV GPLTPEQKEV VSILDSSSRN
310 320 330 340 350
LQKLIEQLLD YNRKQADSAV ELENVELAPL VETVVSAHSL PARAKMMHTD
360 370 380 390 400
VDLKATACLA EPMLLMSVLD NLYSNAVHYG AESGNICLRS SLHGARVYID
410 420 430 440 450
VINTGTPIPQ EERAMIFEPF FQGSHQRKGA VKGSGLGLSI ARDCIRRMQG
460 470
ELYLVDESGQ DVCFRIELPS SKNTK
Length:475
Mass (Da):53,330
Last modified:January 15, 2008 - v3
Checksum:iEBB3F4D821C24AEB
GO

Sequence cautioni

The sequence AAA79818 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U36841 Genomic DNA. Translation: AAA79818.1. Different initiation.
U00096 Genomic DNA. Translation: AAC75609.2.
AP009048 Genomic DNA. Translation: BAE76732.1.
PIRiC65033.
RefSeqiNP_417051.2. NC_000913.3.
WP_001311037.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75609; AAC75609; b2556.
BAE76732; BAE76732; BAE76732.
GeneIDi947013.
KEGGiecj:JW5407.
eco:b2556.
PATRICi32120511. VBIEscCol129921_2658.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U36841 Genomic DNA. Translation: AAA79818.1. Different initiation.
U00096 Genomic DNA. Translation: AAC75609.2.
AP009048 Genomic DNA. Translation: BAE76732.1.
PIRiC65033.
RefSeqiNP_417051.2. NC_000913.3.
WP_001311037.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP52101.
SMRiP52101.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-12052N.
STRINGi511145.b2556.

Proteomic databases

PaxDbiP52101.
PRIDEiP52101.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75609; AAC75609; b2556.
BAE76732; BAE76732; BAE76732.
GeneIDi947013.
KEGGiecj:JW5407.
eco:b2556.
PATRICi32120511. VBIEscCol129921_2658.

Organism-specific databases

EchoBASEiEB3234.
EcoGeneiEG13461. glrK.

Phylogenomic databases

eggNOGiENOG4107WP9. Bacteria.
ENOG410Y4CW. LUCA.
HOGENOMiHOG000284457.
InParanoidiP52101.
KOiK07711.
OMAiINFSHET.
PhylomeDBiP52101.

Enzyme and pathway databases

BioCyciEcoCyc:G7345-MONOMER.
ECOL316407:JW5407-MONOMER.

Miscellaneous databases

PROiP52101.

Family and domain databases

Gene3Di1.10.287.130. 1 hit.
3.30.565.10. 1 hit.
InterProiIPR003660. HAMP_dom.
IPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR004358. Sig_transdc_His_kin-like_C.
[Graphical view]
PfamiPF00672. HAMP. 1 hit.
PF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
[Graphical view]
SUPFAMiSSF47384. SSF47384. 1 hit.
SSF55874. SSF55874. 1 hit.
PROSITEiPS50109. HIS_KIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGLRK_ECOLI
AccessioniPrimary (citable) accession number: P52101
Secondary accession number(s): P76587, Q2MAH4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: January 15, 2008
Last modified: November 2, 2016
This is version 143 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Not required for the regulation of the glmY-glmZ-glmS regulatory cascade by glucosamine-6-phosphate depletion.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.