P52101 (GLRK_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sensor histidine kinase GlrK
EC=2.7.13.3

Gene names

Name:	glrK
Synonyms:	yfhK
Ordered Locus Names:	b2556, JW5407

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	475 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Member of the two-component regulatory system GlrR/GlrK that up-regulates transcription of the glmY sRNA when cells enter the stationary growth phase. Activates GlrR by phosphorylation. Ref.3 Ref.5
Catalytic activity	ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.
Subcellular location	Cell inner membrane; Multi-pass membrane protein.
Post-translational modification	Autophosphorylated. Ref.3
Disruption phenotype	Mutants show decreased amounts of glmY. Ref.5
Miscellaneous	Not required for the regulation of the glmY-glmZ-glmS regulatory cascade by glucosamine-6-phosphate depletion.
Sequence similarities	Contains 1 histidine kinase domain.
Sequence caution	The sequence AAA79818.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Biological process	Two-component regulatory system
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein autophosphorylation Inferred from direct assay Ref.3. Source: EcoCyc
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphorelay sensor kinase activity Inferred from direct assay Ref.3. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 475	475	Sensor histidine kinase GlrK		PRO_0000074915
Regions
	Topological domain	1 – 13	13	Cytoplasmic Potential
	Transmembrane	14 – 34	21	Helical; Potential
	Topological domain	35 – 173	139	Periplasmic Potential
	Transmembrane	174 – 194	21	Helical; Potential
	Topological domain	195 – 475	281	Cytoplasmic Potential
	Domain	256 – 472	217	Histidine kinase
Amino acid modifications
	Modified residue	259	1	Phosphohistidine; by autocatalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	P52101 [UniParc]. Last modified January 15, 2008. Version 3. Checksum: EBB3F4D821C24AEB	FASTA	475	53,330
	10 20 30 40 50 60 MKRWPVFPRS LRQLVMLAFL LILLPLLVLA WQAWQSLNAL SDQAALVNRT TLIDARRSEA 70 80 90 100 110 120 MTNAALEMER SYRQYCVLDD PTLAKVYQSQ RKRYSEMLDA HAGVLPDDKL YQALRQDLHN 130 140 150 160 170 180 LAQLQCNNSG PDAAAAARLE AFASANTEMV QATRTVVFSR GQQLQREIAE RGQYFGWQSL 190 200 210 220 230 240 VLFLVSLVMV LLFTRMIIGP VKNIERMINR LGEGRSLGNS VSFSGPSELR SVGQRILWLS 250 260 270 280 290 300 ERLSWLESQR HQFLRHLSHE LKTPLASMRE GTELLADQVV GPLTPEQKEV VSILDSSSRN 310 320 330 340 350 360 LQKLIEQLLD YNRKQADSAV ELENVELAPL VETVVSAHSL PARAKMMHTD VDLKATACLA 370 380 390 400 410 420 EPMLLMSVLD NLYSNAVHYG AESGNICLRS SLHGARVYID VINTGTPIPQ EERAMIFEPF 430 440 450 460 470 FQGSHQRKGA VKGSGLGLSI ARDCIRRMQG ELYLVDESGQ DVCFRIELPS SKNTK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"Functional characterization in vitro of all two-component signal transduction systems from Escherichia coli." Yamamoto K., Hirao K., Oshima T., Aiba H., Utsumi R., Ishihama A. J. Biol. Chem. 280:1448-1456(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, AUTOPHOSPHORYLATION. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Dual control by perfectly overlapping sigma 54- and sigma 70-promoters adjusts small RNA GlmY expression to different environmental signals." Reichenbach B., Gopel Y., Gorke B. Mol. Microbiol. 74:1054-1070(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE, GENE NAME.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U36841 Genomic DNA. Translation: AAA79818.1. Different initiation. U00096 Genomic DNA. Translation: AAC75609.2. AP009048 Genomic DNA. Translation: BAE76732.1.
PIR	C65033.
RefSeq	NP_417051.2. NC_000913.2. YP_490784.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P52101.
SMR	P52101. Positions 241-472.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-12052N.
STRING	511145.b2556.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC75609; AAC75609; b2556. BAE76732; BAE76732; BAE76732.
GeneID	12930210. 947013.
KEGG	ecj:Y75_p2509. eco:b2556.
PATRIC	32120511. VBIEscCol129921_2658.
Organism-specific databases
EchoBASE	EB3234.
EcoGene	EG13461. glrK.
Phylogenomic databases
eggNOG	COG0642.
HOGENOM	HOG000284457.
KO	K07711.
OMA	INFSHET.
ProtClustDB	CLSK880434.
Enzyme and pathway databases
BioCyc	EcoCyc:G7345-MONOMER. ECOL316407:JW5407-MONOMER.
Gene expression databases
Genevestigator	P52101.
Family and domain databases
Gene3D	1.10.287.130. 1 hit. 3.30.565.10. 1 hit.
InterPro	IPR003660. HAMP_linker_domain. IPR003594. HATPase_ATP-bd. IPR004358. Sig_transdc_His_kin-like_C. IPR003661. Sig_transdc_His_kin_sub1_dim/P. IPR005467. Sig_transdc_His_kinase_core. IPR009082. Sig_transdc_His_kinase_dimeric. [Graphical view]
Pfam	PF00672. HAMP. 1 hit. PF02518. HATPase_c. 1 hit. PF00512. HisKA. 1 hit. [Graphical view]
PRINTS	PR00344. BCTRLSENSOR.
SMART	SM00387. HATPase_c. 1 hit. SM00388. HisKA. 1 hit. [Graphical view]
SUPFAM	SSF55874. ATP_bd_ATPase. 1 hit. SSF47384. His_kin_homodim. 1 hit.
PROSITE	PS50109. HIS_KIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLRK_ECOLI

Accession

Primary (citable) accession number: P52101
Secondary accession number(s): P76587, Q2MAH4

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	January 15, 2008
Last modified:	May 1, 2013
This is version 118 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program