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Protein

Sensor histidine kinase GlrK

Gene

glrK

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Member of the two-component regulatory system GlrR/GlrK that up-regulates transcription of the glmY sRNA when cells enter the stationary growth phase. Activates GlrR by phosphorylation.2 Publications

Miscellaneous

Not required for the regulation of the glmY-glmZ-glmS regulatory cascade by glucosamine-6-phosphate depletion.

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • phosphorelay sensor kinase activity Source: EcoCyc

GO - Biological processi

  • phosphorelay signal transduction system Source: EcoCyc
  • protein autophosphorylation Source: EcoCyc

Keywordsi

Molecular functionKinase, Transferase
Biological processTwo-component regulatory system
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:G7345-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Sensor histidine kinase GlrK (EC:2.7.13.3)
Gene namesi
Name:glrK
Synonyms:yfhK
Ordered Locus Names:b2556, JW5407
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13461 glrK

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 13CytoplasmicSequence analysisAdd BLAST13
Transmembranei14 – 34HelicalSequence analysisAdd BLAST21
Topological domaini35 – 173PeriplasmicSequence analysisAdd BLAST139
Transmembranei174 – 194HelicalSequence analysisAdd BLAST21
Topological domaini195 – 475CytoplasmicSequence analysisAdd BLAST281

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • intracellular Source: GOC
  • plasma membrane Source: EcoCyc

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Mutants show decreased amounts of glmY.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000749151 – 475Sensor histidine kinase GlrKAdd BLAST475

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei259Phosphohistidine; by autocatalysisPROSITE-ProRule annotation1

Post-translational modificationi

Autophosphorylated.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP52101
PRIDEiP52101

PTM databases

iPTMnetiP52101

Interactioni

Protein-protein interaction databases

BioGridi4259204, 3 interactors
DIPiDIP-12052N
STRINGi316385.ECDH10B_2723

Structurei

3D structure databases

ProteinModelPortaliP52101
SMRiP52101
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini256 – 472Histidine kinasePROSITE-ProRule annotationAdd BLAST217

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4107WP9 Bacteria
ENOG410Y4CW LUCA
HOGENOMiHOG000284457
InParanoidiP52101
KOiK07711
OMAiFLRHVSH
PhylomeDBiP52101

Family and domain databases

CDDicd00075 HATPase_c, 1 hit
cd00082 HisKA, 1 hit
Gene3Di3.30.565.10, 1 hit
InterProiView protein in InterPro
IPR003594 HATPase_C
IPR036890 HATPase_C_sf
IPR005467 His_kinase_dom
IPR003661 HisK_dim/P
IPR036097 HisK_dim/P_sf
IPR004358 Sig_transdc_His_kin-like_C
PfamiView protein in Pfam
PF02518 HATPase_c, 1 hit
PF00512 HisKA, 1 hit
PRINTSiPR00344 BCTRLSENSOR
SMARTiView protein in SMART
SM00387 HATPase_c, 1 hit
SM00388 HisKA, 1 hit
SUPFAMiSSF47384 SSF47384, 1 hit
SSF55874 SSF55874, 1 hit
PROSITEiView protein in PROSITE
PS50109 HIS_KIN, 1 hit

Sequencei

Sequence statusi: Complete.

P52101-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRWPVFPRS LRQLVMLAFL LILLPLLVLA WQAWQSLNAL SDQAALVNRT
60 70 80 90 100
TLIDARRSEA MTNAALEMER SYRQYCVLDD PTLAKVYQSQ RKRYSEMLDA
110 120 130 140 150
HAGVLPDDKL YQALRQDLHN LAQLQCNNSG PDAAAAARLE AFASANTEMV
160 170 180 190 200
QATRTVVFSR GQQLQREIAE RGQYFGWQSL VLFLVSLVMV LLFTRMIIGP
210 220 230 240 250
VKNIERMINR LGEGRSLGNS VSFSGPSELR SVGQRILWLS ERLSWLESQR
260 270 280 290 300
HQFLRHLSHE LKTPLASMRE GTELLADQVV GPLTPEQKEV VSILDSSSRN
310 320 330 340 350
LQKLIEQLLD YNRKQADSAV ELENVELAPL VETVVSAHSL PARAKMMHTD
360 370 380 390 400
VDLKATACLA EPMLLMSVLD NLYSNAVHYG AESGNICLRS SLHGARVYID
410 420 430 440 450
VINTGTPIPQ EERAMIFEPF FQGSHQRKGA VKGSGLGLSI ARDCIRRMQG
460 470
ELYLVDESGQ DVCFRIELPS SKNTK
Length:475
Mass (Da):53,330
Last modified:January 15, 2008 - v3
Checksum:iEBB3F4D821C24AEB
GO

Sequence cautioni

The sequence AAA79818 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U36841 Genomic DNA Translation: AAA79818.1 Different initiation.
U00096 Genomic DNA Translation: AAC75609.2
AP009048 Genomic DNA Translation: BAE76732.1
PIRiC65033
RefSeqiNP_417051.2, NC_000913.3
WP_001311037.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75609; AAC75609; b2556
BAE76732; BAE76732; BAE76732
GeneIDi947013
KEGGiecj:JW5407
eco:b2556
PATRICifig|511145.12.peg.2658

Similar proteinsi

Entry informationi

Entry nameiGLRK_ECOLI
AccessioniPrimary (citable) accession number: P52101
Secondary accession number(s): P76587, Q2MAH4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: January 15, 2008
Last modified: March 28, 2018
This is version 151 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health