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Reviewed, UniProtKB/Swiss-Prot P52097 (TILS_ECOLI)

Last modified February 9, 2010. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    tRNA(Ile)-lysidine synthase
    EC=6.3.4.-
Alternative name(s):
    tRNA(Ile)-lysidine synthetase
    tRNA(Ile)-2-lysyl-cytidine synthase
Gene names
Name: tilS
Synonyms: mesJ, yaeN
Ordered Locus Names: b0188, JW0183
OrganismEscherichia coli (strain K12) [Complete proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

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Protein attributes

Sequence length432 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionine to isoleucine. This enzyme is essential for viability. HAMAP MF_01161

Subcellular location

Cytoplasm Probable HAMAP MF_01161.

Domain

The N-terminal region contains the highly conserved SGGXDS motif, predicted to be a P-loop motif involved in ATP binding. HAMAP MF_01161

Sequence similarities

Belongs to the tRNA(Ile)-lysidine synthase family.

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Ontologies

Keywords
   Biological processtRNA processing
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical term3D-structure
Complete proteome
Gene Ontology (GO)
   Biological processtranslation

Inferred from electronic annotation. Source: InterPro

wobble base lysidine biosynthesis Ref.7

Inferred from mutant phenotype. Source: UniProtKB

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

tRNA(Ile)-lysidine synthase activity Ref.7

Inferred from direct assay. Source: UniProtKB

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 432432tRNA(Ile)-lysidine synthase HAMAP MF_01161
PRO_0000181689

Regions

Nucleotide binding20 – 256ATP Potential

Secondary structure

1 ................................................................................ 432
Helix Strand Turn

Details...

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Sequences

Sequence LengthMass (Da)Tools
P52097-1 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 26946DC7220B5A40

FASTA43248,204
        10         20         30         40         50         60 
MTLTLNRQLL TSRQILVAFS GGLDSTVLLH QLVQWRTENP GVALRAIHVH HGLSANADAW 

        70         80         90        100        110        120 
VTHCENVCQQ WQVPLVVERV QLAQEGLGIE AQARQARYQA FARTLLPGEV LVTAQHLDDQ 

       130        140        150        160        170        180 
CETFLLALKR GSGPAGLSAM AEVSEFAGTR LIRPLLARTR GELVQWARQY DLRWIEDESN 

       190        200        210        220        230        240 
QDDSYDRNFL RLRVVPLLQQ RWPHFAEATA RSAALCAEQE SLLDELLADD LAHCQSPQGT 

       250        260        270        280        290        300 
LQIVPMLAMS DARRAAIIRR WLAGQNAPMP SRDALVRIWQ EVALAREDAS PCLRLGAFEI 

       310        320        330        340        350        360 
RRYQSQLWWI KSVTGQSENI VPWQTWLQPL ELPAGLGSVQ LNAGGDIRPP RADEAVSVRF 

       370        380        390        400        410        420 
KAPGLLHIVG RNGGRKLKKI WQELGVPPWL RDTTPLLFYG ETLIAAAGVF VTQEGVAEGE 

       430 
NGVSFVWQKT LS

« Hide

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References

« Hide 'large scale' references
[1]Yamamoto Y.
Submitted (DEC-1995) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]"An Escherichia coli gene (yaeO) suppresses temperature-sensitive mutations in essential genes by modulating Rho-dependent transcription termination."
Pichoff S., Alibaud L., Guedant A., Castanie M.-P., Bouche J.-P.
Mol. Microbiol. 29:859-869(1998) [PubMed: 9723924] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12.
[3]"Systematic sequencing of the Escherichia coli genome: analysis of the 4.0 - 6.0 min (189,987 - 281,416bp) region."
Takemoto K., Mori H., Murayama N., Kataoka K., Yano M., Itoh T., Yamamoto Y., Inokuchi H., Miki T., Hatada E., Fukuda R., Ichihara S., Mizuno T., Makino K., Nakata A., Yura T., Sampei G., Mizobuchi K.
Submitted (FEB-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]"Sequence of minutes 4-25 of Escherichia coli."
Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[5]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[6]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[7]"An RNA-modifying enzyme that governs both the codon and amino acid specificities of isoleucine tRNA."
Soma A., Ikeuchi Y., Kanemasa S., Kobayashi K., Ogasawara N., Ote T., Kato J., Watanabe K., Sekine Y., Suzuki T.
Mol. Cell 12:689-698(2003) [PubMed: 14527414] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
[8]"Structure of the MesJ Pp-ATPase from Escherichia coli."
Gu M., Burling T., Lima C.D.
Submitted (JAN-2003) to the PDB data bank
Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS).

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		D49445 Genomic DNA. Translation: BAA08428.1.
Z50870 Genomic DNA. Translation: CAA90751.1.
U70214 Genomic DNA. Translation: AAB08617.1.
U00096 Genomic DNA. Translation: AAC73299.1.
AP009048 Genomic DNA. Translation: BAA77863.1.
BR000032 Genomic DNA. Translation: FAA00003.1.
PIRD64743.
RefSeqAP_000848.1.
NP_414730.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1NI5X-ray2.65A1-432[»]
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-10190N.
STRINGP52097.

Genome annotation databases

GeneID944889.
GenomeReviewsGene locus JW0183 in contig AP009048_GR.
Gene locus b0188 in contig U00096_GR.
KEGGecj:JW0183.
eco:b0188.

Organism-specific databases

EchoBASEEB3011.
EcoGeneEG13220. tilS.
CMRSearch...

Phylogenomic databases

eggNOGCOG0037.
HOGENOMHBG668702.
OMAGIPPWRR.

Enzyme and pathway databases

BioCycEcoCyc:G6096-MONOMER.
ECOL168927:B0188-MONOMER.

Gene expression databases

GenevestigatorP52097.

Family and domain databases

HAMAPMF_01161. tRNA(Ile)-lys_synt.
[Tree]
InterProIPR012094. Lysidine-tRNA-synth.
IPR012796. Lysidine-tRNA-synth_C.
IPR012795. Lysidine-tRNA-synth_N.
IPR015262. Lysidine-tRNA-synth_subst-bd.
IPR011063. PP_loop.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
Gene3DG3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHERPTHR11807:SF2. ATPase_MesJ_YaeO. 1 hit.
PfamPF01171. ATP_bind_3. 1 hit.
PF09179. DUF1946. 1 hit.
PF11734. TilS_C. 1 hit.
[Graphical view]
TIGRFAMsTIGR02433. lysidine_TilS_C. 1 hit.
TIGR02432. lysidine_TilS_N. 1 hit.
ProtoNetSearch...

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Entry information

Entry nameTILS_ECOLI
AccessionPrimary (citable) accession number: P52097
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: February 9, 2010
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Uncharacterized protein families (UPF)

List of uncharacterized protein family (UPF) entries

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents