tRNA(Ile)-lysidine synthase - Escherichia coli (strain K12)

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P52097 (TILS_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 100. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
tRNA(Ile)-lysidine synthase
EC=6.3.4.19

Alternative name(s):
tRNA(Ile)-2-lysyl-cytidine synthase
tRNA(Ile)-lysidine synthetase

Gene names

Name:	tilS
Synonyms:	mesJ, yaeN
Ordered Locus Names:	b0188, JW0183

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	432 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionine to isoleucine. This enzyme is essential for viability. HAMAP MF_01161
Catalytic activity	(tRNA(Ile2))-cytidine(34) + L-lysine + ATP = (tRNA(Ile2))-lysidine(34) + AMP + diphosphate + H₂O. Ref.7
Subcellular location	Cytoplasm Probable HAMAP MF_01161.
Domain	The N-terminal region contains the highly conserved SGGXDS motif, predicted to be a P-loop motif involved in ATP binding. HAMAP MF_01161
Sequence similarities	Belongs to the tRNA(Ile)-lysidine synthase family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	wobble base lysidine biosynthesis Inferred from mutant phenotype Ref.7. Source: EcoCyc
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW tRNA(Ile)-lysidine synthase activity Inferred from direct assay Ref.7. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 432

432

tRNA(Ile)-lysidine synthase HAMAP MF_01161

PRO_0000181689

Regions

Nucleotide binding

20 – 25

ATP Potential

Secondary structure

432

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P52097 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 26946DC7220B5A40
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FASTA

432

48,204

        10         20         30         40         50         60 
MTLTLNRQLL TSRQILVAFS GGLDSTVLLH QLVQWRTENP GVALRAIHVH HGLSANADAW 

        70         80         90        100        110        120 
VTHCENVCQQ WQVPLVVERV QLAQEGLGIE AQARQARYQA FARTLLPGEV LVTAQHLDDQ 

       130        140        150        160        170        180 
CETFLLALKR GSGPAGLSAM AEVSEFAGTR LIRPLLARTR GELVQWARQY DLRWIEDESN 

       190        200        210        220        230        240 
QDDSYDRNFL RLRVVPLLQQ RWPHFAEATA RSAALCAEQE SLLDELLADD LAHCQSPQGT 

       250        260        270        280        290        300 
LQIVPMLAMS DARRAAIIRR WLAGQNAPMP SRDALVRIWQ EVALAREDAS PCLRLGAFEI 

       310        320        330        340        350        360 
RRYQSQLWWI KSVTGQSENI VPWQTWLQPL ELPAGLGSVQ LNAGGDIRPP RADEAVSVRF 

       370        380        390        400        410        420 
KAPGLLHIVG RNGGRKLKKI WQELGVPPWL RDTTPLLFYG ETLIAAAGVF VTQEGVAEGE 

       430 
NGVSFVWQKT LS

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Yamamoto Y. Submitted (DEC-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"An Escherichia coli gene (yaeO) suppresses temperature-sensitive mutations in essential genes by modulating Rho-dependent transcription termination." Pichoff S., Alibaud L., Guedant A., Castanie M.-P., Bouche J.-P. Mol. Microbiol. 29:859-869(1998) [PubMed: 9723924] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[3]	"Systematic sequencing of the Escherichia coli genome: analysis of the 4.0 - 6.0 min (189,987 - 281,416bp) region." Takemoto K., Mori H., Murayama N., Kataoka K., Yano M., Itoh T., Yamamoto Y., Inokuchi H., Miki T., Hatada E., Fukuda R., Ichihara S., Mizuno T., Makino K., Nakata A., Yura T., Sampei G., Mizobuchi K. Submitted (FEB-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Sequence of minutes 4-25 of Escherichia coli." Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[6]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[7]	"An RNA-modifying enzyme that governs both the codon and amino acid specificities of isoleucine tRNA." Soma A., Ikeuchi Y., Kanemasa S., Kobayashi K., Ogasawara N., Ote T., Kato J., Watanabe K., Sekine Y., Suzuki T. Mol. Cell 12:689-698(2003) [PubMed: 14527414] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CATALYTIC ACTIVITY, CHARACTERIZATION.
[8]	"Structure of the MesJ Pp-ATPase from Escherichia coli." Gu M., Burling T., Lima C.D. Submitted (JAN-2003) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

D49445 Genomic DNA. Translation: BAA08428.1.
Z50870 Genomic DNA. Translation: CAA90751.1.
U70214 Genomic DNA. Translation: AAB08617.1.
U00096 Genomic DNA. Translation: AAC73299.1.
AP009048 Genomic DNA. Translation: BAA77863.1.
BR000032 Genomic DNA. Translation: FAA00003.1.

PIR

D64743.

RefSeq

NP_414730.1. NC_000913.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1NI5	X-ray	2.65	A	1-432	[»]

ProteinModelPortal

P52097.

SMR

P52097. Positions 1-432.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-10190N.

IntAct

P52097. 15 interactions.

MINT

MINT-1261006.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBESCT00000004816; EBESCP00000004816; EBESCG00000003932.
EBESCT00000015119; EBESCP00000014410; EBESCG00000014179.

GeneID

944889.

GenomeReviews

Gene locus JW0183 in contig AP009048_GR.
Gene locus b0188 in contig U00096_GR.

KEGG

ecj:JW0183.
eco:b0188.

PATRIC

32115487. VBIEscCol129921_0195.

Organism-specific databases

EchoBASE

EB3011.

EcoGene

EG13220. tilS.

Phylogenomic databases

eggNOG

COG0037.

GeneTree

EBGT00050000009088.

HOGENOM

HBG668702.

OMA

IPPWRRD.

PhylomeDB

P52097.

ProtClustDB

PRK10660.

Enzyme and pathway databases

BioCyc

EcoCyc:G6096-MONOMER.
MetaCyc:G6096-MONOMER.

Gene expression databases

Genevestigator

P52097.

Family and domain databases

HAMAP

MF_01161. tRNA(Ile)-lys_synt.
[Tree]

InterPro

IPR012094. Lysidine-tRNA-synth.
IPR012796. Lysidine-tRNA-synth_C.
IPR012795. Lysidine-tRNA-synth_N.
IPR015262. Lysidine-tRNA-synth_subst-bd.
IPR020825. Phe-tRNA_synthase_B3/B4.
IPR014729. Rossmann-like_a/b/a_fold.
IPR011063. tRNA-lysidine/thiocyt_synth.
[Graphical view]

Gene3D

G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.

K04075.

Pfam

PF01171. ATP_bind_3. 1 hit.
PF09179. TilS. 1 hit.
PF11734. TilS_C. 1 hit.
[Graphical view]

SMART

SM00977. TilS_C. 1 hit.
[Graphical view]

SUPFAM

SSF56037. B3_4. 1 hit.

TIGRFAMs

TIGR02433. Lysidine_TilS_C. 1 hit.
TIGR02432. Lysidine_TilS_N. 1 hit.

ProtoNet

Search...

Entry information

Entry name

TILS_ECOLI

Accession

Primary (citable) accession number: P52097

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	January 25, 2012
This is version 100 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Uncharacterized protein families (UPF)

List of uncharacterized protein family (UPF) entries

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents