Reviewed, UniProtKB/Swiss-Prot P52086 (COBC_ECOLI)

Names and origin

Protein names

Recommended name:
    Alpha-ribazole phosphatase
    EC=3.1.3.73
Alternative name(s):
    Alpha-ribazole-5'-phosphate phosphatase

Gene names

Name:	cobC
Synonyms:	phpB
Ordered Locus Names:	b0638, JW0633

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	203 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Converts N1-(5-phospho-alpha-D-ribosyl)-5,6-dimethylbenzimidazole into N1-alpha-D-ribosyl-5,6-dimethylbenzimidazole; involved in the assembly of the nucleotide loop of cobalamin.
Catalytic activity	Alpha-ribazole 5'-phosphate + H2O = alpha-ribazole + phosphate.
Pathway	Nucleoside biosynthesis; alpha-ribazole biosynthesis; alpha-ribazole from 5,6-dimethylbenzimidazole: step 2/2.
Sequence similarities	Belongs to the phosphoglycerate mutase family.

Ontologies

Keywords
Biological process	Cobalamin biosynthesis
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cobalamin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alpha-ribazole phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 203	203	Alpha-ribazole phosphatase		PRO_0000179954
Sites
	Active site	8	1	Tele-phosphohistidine intermediate By similarity
	Site	149	1	Interaction with carboxyl group of phosphoglycerates By similarity

Sequences

	Sequence		Length	Mass (Da)
	P52086-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 9034B19DC366E1D0	FASTA	203	23,308
	10 20 30 40 50 60 MRLWLIRHGE TQANIDGLYS GHAPTPLTAR GIEQAQNLHT LLHGVSFDLV LCSELERAQH 70 80 90 100 110 120 TARLVLSDRQ LPVQIIPELN EMFFGDWEMR HHRDLMQEDA ENYSAWCNDW QHAIPTNGEG 130 140 150 160 170 180 FQAFSQRVER FIARLSEFQH YQNILVVSHQ GVLSLLIARL IGMPAEAMWH FRVDQGCWSA 190 200 IDINQKFATL RVLNSRAIGV ENA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Addinall S.G., Donachie W.D. Submitted (APR-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. , Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T. DNA Res. 3:137-155(1996) [PubMed: 8905232] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"Sequence of minutes 4-25 of Escherichia coli." Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U23163 Genomic DNA. Translation: AAA64853.1. U82598 Genomic DNA. Translation: AAB40839.1. Different initiation. U00096 Genomic DNA. Translation: AAC73739.1. AP009048 Genomic DNA. Translation: BAA35285.1.
PIR	D64798.
RefSeq	AP_001287.1. NP_415171.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	945246.
GenomeReviews	Gene locus JW0633 in contig AP009048_GR. Gene locus b0638 in contig U00096_GR.
KEGG	ecj:JW0633. eco:b0638.
Organism-specific databases
EchoBASE	EB3029.
EcoGene	EG13240. cobC.
CMR	Search...
Phylogenomic databases
HOGENOM	P52086.
OMA	P52086. HTARRVL.
Enzyme and pathway databases
BioCyc	EcoCyc:RIBAZOLEPHOSPHAT-MON.
Family and domain databases
InterPro	IPR001345. PG/BPGM_mutase. IPR013078. PG_mutase. IPR017578. Ribazole_CobC. [Graphical view]
Pfam	PF00300. PGAM. 1 hit. [Graphical view]
TIGRFAMs	TIGR03162. ribazole_cobC. 1 hit.
PROSITE	PS00175. PG_MUTASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

COBC_ECOLI

Accession

Primary (citable) accession number: P52086
Secondary accession number(s): P77109

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	June 16, 2009
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)