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Protein

Adenosylcobalamin/alpha-ribazole phosphatase

Gene

cobC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of adenosylcobalamin 5'-phosphate to adenosylcobalamin (vitamin B12); involved in the assembly of the nucleotide loop of cobalamin. Also catalyzes the hydrolysis of the phospho group from alpha-ribazole 5'-phosphate to form alpha-ribazole.By similarity

Catalytic activityi

Adenosylcobalamin 5'-phosphate + H2O = coenzyme B12 + phosphate.By similarity
Alpha-ribazole 5'-phosphate + H2O = alpha-ribazole + phosphate.By similarity

Pathwayi: alpha-ribazole biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes alpha-ribazole from 5,6-dimethylbenzimidazole.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase (cobT)
  2. Adenosylcobalamin/alpha-ribazole phosphatase (cobC)
This subpathway is part of the pathway alpha-ribazole biosynthesis, which is itself part of Nucleoside biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes alpha-ribazole from 5,6-dimethylbenzimidazole, the pathway alpha-ribazole biosynthesis and in Nucleoside biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei8Tele-phosphohistidine intermediateBy similarity1
Active sitei81Proton donor/acceptorBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Cobalamin biosynthesis

Enzyme and pathway databases

BioCyciEcoCyc:RIBAZOLEPHOSPHAT-MONOMER.
ECOL316407:JW0633-MONOMER.
MetaCyc:RIBAZOLEPHOSPHAT-MONOMER.
UniPathwayiUPA00061; UER00517.

Names & Taxonomyi

Protein namesi
Recommended name:
Adenosylcobalamin/alpha-ribazole phosphatase (EC:3.1.3.73By similarity)
Alternative name(s):
Adenosylcobalamin phosphatase
Alpha-ribazole-5'-phosphate phosphatase
Gene namesi
Name:cobC
Synonyms:phpB
Ordered Locus Names:b0638, JW0633
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13240. cobC.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001799541 – 203Adenosylcobalamin/alpha-ribazole phosphataseAdd BLAST203

Proteomic databases

PaxDbiP52086.
PRIDEiP52086.

Interactioni

Protein-protein interaction databases

IntActiP52086. 3 interactors.
STRINGi511145.b0638.

Structurei

3D structure databases

ProteinModelPortaliP52086.
SMRiP52086.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the phosphoglycerate mutase family.Curated

Phylogenomic databases

eggNOGiENOG4108TGR. Bacteria.
COG0406. LUCA.
HOGENOMiHOG000221683.
InParanoidiP52086.
KOiK02226.
OMAiEVERSHC.
PhylomeDBiP52086.

Family and domain databases

CDDicd07067. HP_PGM_like. 1 hit.
Gene3Di3.40.50.1240. 1 hit.
InterProiIPR013078. His_Pase_superF_clade-1.
IPR029033. His_PPase_superfam.
IPR001345. PG/BPGM_mutase_AS.
IPR017578. Ribazole_CobC.
[Graphical view]
PfamiPF00300. His_Phos_1. 1 hit.
[Graphical view]
SMARTiSM00855. PGAM. 1 hit.
[Graphical view]
SUPFAMiSSF53254. SSF53254. 1 hit.
TIGRFAMsiTIGR03162. ribazole_cobC. 1 hit.
PROSITEiPS00175. PG_MUTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P52086-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLWLIRHGE TQANIDGLYS GHAPTPLTAR GIEQAQNLHT LLHGVSFDLV
60 70 80 90 100
LCSELERAQH TARLVLSDRQ LPVQIIPELN EMFFGDWEMR HHRDLMQEDA
110 120 130 140 150
ENYSAWCNDW QHAIPTNGEG FQAFSQRVER FIARLSEFQH YQNILVVSHQ
160 170 180 190 200
GVLSLLIARL IGMPAEAMWH FRVDQGCWSA IDINQKFATL RVLNSRAIGV

ENA
Length:203
Mass (Da):23,308
Last modified:October 1, 1996 - v1
Checksum:i9034B19DC366E1D0
GO

Sequence cautioni

The sequence AAB40839 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U23163 Genomic DNA. Translation: AAA64853.1.
U82598 Genomic DNA. Translation: AAB40839.1. Different initiation.
U00096 Genomic DNA. Translation: AAC73739.1.
AP009048 Genomic DNA. Translation: BAA35285.1.
PIRiD64798.
RefSeqiNP_415171.1. NC_000913.3.
WP_001241872.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73739; AAC73739; b0638.
BAA35285; BAA35285; BAA35285.
GeneIDi945246.
KEGGiecj:JW0633.
eco:b0638.
PATRICi32116459. VBIEscCol129921_0669.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U23163 Genomic DNA. Translation: AAA64853.1.
U82598 Genomic DNA. Translation: AAB40839.1. Different initiation.
U00096 Genomic DNA. Translation: AAC73739.1.
AP009048 Genomic DNA. Translation: BAA35285.1.
PIRiD64798.
RefSeqiNP_415171.1. NC_000913.3.
WP_001241872.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP52086.
SMRiP52086.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP52086. 3 interactors.
STRINGi511145.b0638.

Proteomic databases

PaxDbiP52086.
PRIDEiP52086.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73739; AAC73739; b0638.
BAA35285; BAA35285; BAA35285.
GeneIDi945246.
KEGGiecj:JW0633.
eco:b0638.
PATRICi32116459. VBIEscCol129921_0669.

Organism-specific databases

EchoBASEiEB3029.
EcoGeneiEG13240. cobC.

Phylogenomic databases

eggNOGiENOG4108TGR. Bacteria.
COG0406. LUCA.
HOGENOMiHOG000221683.
InParanoidiP52086.
KOiK02226.
OMAiEVERSHC.
PhylomeDBiP52086.

Enzyme and pathway databases

UniPathwayiUPA00061; UER00517.
BioCyciEcoCyc:RIBAZOLEPHOSPHAT-MONOMER.
ECOL316407:JW0633-MONOMER.
MetaCyc:RIBAZOLEPHOSPHAT-MONOMER.

Miscellaneous databases

PROiP52086.

Family and domain databases

CDDicd07067. HP_PGM_like. 1 hit.
Gene3Di3.40.50.1240. 1 hit.
InterProiIPR013078. His_Pase_superF_clade-1.
IPR029033. His_PPase_superfam.
IPR001345. PG/BPGM_mutase_AS.
IPR017578. Ribazole_CobC.
[Graphical view]
PfamiPF00300. His_Phos_1. 1 hit.
[Graphical view]
SMARTiSM00855. PGAM. 1 hit.
[Graphical view]
SUPFAMiSSF53254. SSF53254. 1 hit.
TIGRFAMsiTIGR03162. ribazole_cobC. 1 hit.
PROSITEiPS00175. PG_MUTASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCOBC_ECOLI
AccessioniPrimary (citable) accession number: P52086
Secondary accession number(s): P77109
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.