P52074 (GLCF_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycolate oxidase iron-sulfur subunit

Gene names

Name:	glcF
Synonyms:	gox, yghL
Ordered Locus Names:	b4467, JW5486

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	407 AA.
Sequence status	Complete.
Protein existence	Predicted

General annotation (Comments)

Sequence similarities	Contains 2 4Fe-4S ferredoxin-type domains.
Sequence caution	The sequence AAA69145.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Electron transport Transport
Domain	Repeat
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW glycolate catabolic process Inferred from mutant phenotype Ref.1. Source: EcoCyc transport Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: InterPro glycolate dehydrogenase activity Inferred from mutant phenotype Ref.1. Source: EcoCyc metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 407	407	Glycolate oxidase iron-sulfur subunit		PRO_0000159258
Regions
	Domain	14 – 47	34	4Fe-4S ferredoxin-type 1
	Domain	66 – 95	30	4Fe-4S ferredoxin-type 2
Sites
	Metal binding	25	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	28	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	31	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	35	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	75	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	78	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	81	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	85	1	Iron-sulfur 1 (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	P52074 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: F983C893063F76DC	FASTA	407	45,110
	10 20 30 40 50 60 MQTQLTEEMR QNARALEADS ILRACVHCGF CTATCPTYQL LGDELDGPRG RIYLIKQVLE 70 80 90 100 110 120 GNEVTLKTQE HLDRCLTCRN CETTCPSGVR YHNLLDIGRD IVEQKVKRPL PERILREGLR 130 140 150 160 170 180 QVVPRPAVFR ALTQVGLVLR PFLPEQVRAK LPAETVKAKP RPPLRHKRRV LMLEGCAQPT 190 200 210 220 230 240 LSPNTNAATA RVLDRLGISV MPANEAGCCG AVDYHLNAQE KGLARARNNI DAWWPAIEAG 250 260 270 280 290 300 AEAILQTASG CGAFVKEYGQ MLKNDALYAD KARQVSELAV DLVELLREEP LEKLAIRGDK 310 320 330 340 350 360 KLAFHCPCTL QHAQKLNGEV EKVLLRLGFT LTDVPDSHLC CGSAGTYALT HPDLARQLRD 370 380 390 400 NKMNALESGK PEMIVTANIG CQTHLASAGR TSVRHWIEIV EQALEKE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"glc locus of Escherichia coli: characterization of genes encoding the subunits of glycolate oxidase and the glc regulator protein." Pellicer M.T., Badia J., Aguilar J.T., Baldoma L. J. Bacteriol. 178:2051-2059(1996) [PubMed: 8606183] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Escherichia coli K-12: a cooperatively developed annotation snapshot -- 2005." Riley M., Abe T., Arnaud M.B., Berlyn M.K.B., Blattner F.R., Chaudhuri R.R., Glasner J.D., Horiuchi T., Keseler I.M., Kosuge T., Mori H., Perna N.T., Plunkett G. III, Rudd K.E., Serres M.H., Thomas G.H., Thomson N.R., Wishart D., Wanner B.L. Nucleic Acids Res. 34:1-9(2006) [PubMed: 16397293] [Abstract] Cited for: SEQUENCE REVISION.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L43490 Genomic DNA. Translation: AAB02532.1. U28377 Genomic DNA. Translation: AAA69145.1. Different initiation. U00096 Genomic DNA. Translation: AAT48157.1. AP009048 Genomic DNA. Translation: BAE77038.1.
RefSeq	YP_026190.1. NC_000913.2.
3D structure databases
ProteinModelPortal	P52074.
SMR	P52074. Positions 22-91.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000001800; EBESCP00000001800; EBESCG00000001485. EBESCT00000014289; EBESCP00000013580; EBESCG00000013351.
GeneID	2847717.
GenomeReviews	Gene locus JW5486 in contig AP009048_GR. Gene locus b4467 in contig U00096_GR.
KEGG	ecj:JW5486. eco:b4467.
PATRIC	32121370. VBIEscCol129921_3072.
Organism-specific databases
EchoBASE	EB3076.
EcoGene	EG13291. glcF.
Phylogenomic databases
eggNOG	COG0247.
GeneTree	EBGT00050000011307.
HOGENOM	HBG646784.
OMA	DYGHMLR.
PhylomeDB	P52074.
ProtClustDB	PRK11274.
Enzyme and pathway databases
BioCyc	EcoCyc:MONOMER0-561. MetaCyc:MONOMER0-561.
Gene expression databases
Genevestigator	P52074.
Family and domain databases
InterPro	IPR001450. 4Fe4S-bd_dom. IPR017896. 4Fe4S_Fe-S-bd. IPR017900. 4Fe4S_Fe_S_CS. IPR004017. Cys_rich_dom. IPR012285. Fum_reductase_C. IPR012257. Glc_ox_4Fe-4S. [Graphical view]
Gene3D	G3DSA:1.10.1060.10. Fum_reductase_C. 1 hit.
KO	K11473.
Pfam	PF02754. CCG. 2 hits. PF12838. Fer4_7. 1 hit. [Graphical view]
PIRSF	PIRSF000139. Glc_ox_4Fe-4S. 1 hit.
PROSITE	PS00198. 4FE4S_FER_1. 2 hits. PS51379. 4FE4S_FER_2. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLCF_ECOLI

Accession

Primary (citable) accession number: P52074
Secondary accession number(s): P76654, Q2M9L8

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	January 25, 2012
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program