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Protein

Glycolate oxidase iron-sulfur subunit

Gene

glcF

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi25 – 251Iron-sulfur 1 (4Fe-4S)By similarity
Metal bindingi28 – 281Iron-sulfur 1 (4Fe-4S)By similarity
Metal bindingi31 – 311Iron-sulfur 1 (4Fe-4S)By similarity
Metal bindingi35 – 351Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi75 – 751Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi78 – 781Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi81 – 811Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi85 – 851Iron-sulfur 1 (4Fe-4S)By similarity

GO - Molecular functioni

  • 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  • glycolate dehydrogenase activity Source: EcoCyc
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • glycolate catabolic process Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Electron transport, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:MONOMER0-561.
ECOL316407:JW5486-MONOMER.
MetaCyc:MONOMER0-561.
RETL1328306-WGS:GSTH-5076-MONOMER.
RETL1328306-WGS:GSTH-819-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycolate oxidase iron-sulfur subunit
Gene namesi
Name:glcF
Synonyms:gox, yghL
Ordered Locus Names:b4467, JW5486
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13291. glcF.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 407407Glycolate oxidase iron-sulfur subunitPRO_0000159258Add
BLAST

Proteomic databases

PaxDbiP52074.
PRIDEiP52074.

Interactioni

Protein-protein interaction databases

BioGridi4262366. 14 interactions.
STRINGi511145.b4467.

Structurei

3D structure databases

ProteinModelPortaliP52074.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini14 – 47344Fe-4S ferredoxin-type 1PROSITE-ProRule annotationAdd
BLAST
Domaini66 – 95304Fe-4S ferredoxin-type 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 2 4Fe-4S ferredoxin-type domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG4105CHG. Bacteria.
COG0247. LUCA.
HOGENOMiHOG000256469.
InParanoidiP52074.
KOiK11473.
OMAiGHTLKEY.
OrthoDBiEOG6WT8B4.
PhylomeDBiP52074.

Family and domain databases

InterProiIPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR004017. Cys_rich_dom.
IPR012257. Glc_ox_4Fe-4S.
[Graphical view]
PfamiPF02754. CCG. 2 hits.
PF13183. Fer4_8. 1 hit.
[Graphical view]
PIRSFiPIRSF000139. Glc_ox_4Fe-4S. 1 hit.
PROSITEiPS00198. 4FE4S_FER_1. 2 hits.
PS51379. 4FE4S_FER_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P52074-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQTQLTEEMR QNARALEADS ILRACVHCGF CTATCPTYQL LGDELDGPRG
60 70 80 90 100
RIYLIKQVLE GNEVTLKTQE HLDRCLTCRN CETTCPSGVR YHNLLDIGRD
110 120 130 140 150
IVEQKVKRPL PERILREGLR QVVPRPAVFR ALTQVGLVLR PFLPEQVRAK
160 170 180 190 200
LPAETVKAKP RPPLRHKRRV LMLEGCAQPT LSPNTNAATA RVLDRLGISV
210 220 230 240 250
MPANEAGCCG AVDYHLNAQE KGLARARNNI DAWWPAIEAG AEAILQTASG
260 270 280 290 300
CGAFVKEYGQ MLKNDALYAD KARQVSELAV DLVELLREEP LEKLAIRGDK
310 320 330 340 350
KLAFHCPCTL QHAQKLNGEV EKVLLRLGFT LTDVPDSHLC CGSAGTYALT
360 370 380 390 400
HPDLARQLRD NKMNALESGK PEMIVTANIG CQTHLASAGR TSVRHWIEIV

EQALEKE
Length:407
Mass (Da):45,110
Last modified:October 1, 1996 - v1
Checksum:iF983C893063F76DC
GO

Sequence cautioni

The sequence AAA69145.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L43490 Genomic DNA. Translation: AAB02532.1.
U28377 Genomic DNA. Translation: AAA69145.1. Different initiation.
U00096 Genomic DNA. Translation: AAT48157.1.
AP009048 Genomic DNA. Translation: BAE77038.1.
RefSeqiWP_001194661.1. NZ_LN832404.1.
YP_026190.1. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiAAT48157; AAT48157; b4467.
BAE77038; BAE77038; BAE77038.
GeneIDi2847717.
KEGGiecj:JW5486.
eco:b4467.
PATRICi32121370. VBIEscCol129921_3072.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L43490 Genomic DNA. Translation: AAB02532.1.
U28377 Genomic DNA. Translation: AAA69145.1. Different initiation.
U00096 Genomic DNA. Translation: AAT48157.1.
AP009048 Genomic DNA. Translation: BAE77038.1.
RefSeqiWP_001194661.1. NZ_LN832404.1.
YP_026190.1. NC_000913.3.

3D structure databases

ProteinModelPortaliP52074.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262366. 14 interactions.
STRINGi511145.b4467.

Proteomic databases

PaxDbiP52074.
PRIDEiP52074.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAT48157; AAT48157; b4467.
BAE77038; BAE77038; BAE77038.
GeneIDi2847717.
KEGGiecj:JW5486.
eco:b4467.
PATRICi32121370. VBIEscCol129921_3072.

Organism-specific databases

EchoBASEiEB3076.
EcoGeneiEG13291. glcF.

Phylogenomic databases

eggNOGiENOG4105CHG. Bacteria.
COG0247. LUCA.
HOGENOMiHOG000256469.
InParanoidiP52074.
KOiK11473.
OMAiGHTLKEY.
OrthoDBiEOG6WT8B4.
PhylomeDBiP52074.

Enzyme and pathway databases

BioCyciEcoCyc:MONOMER0-561.
ECOL316407:JW5486-MONOMER.
MetaCyc:MONOMER0-561.
RETL1328306-WGS:GSTH-5076-MONOMER.
RETL1328306-WGS:GSTH-819-MONOMER.

Miscellaneous databases

PROiP52074.

Family and domain databases

InterProiIPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR004017. Cys_rich_dom.
IPR012257. Glc_ox_4Fe-4S.
[Graphical view]
PfamiPF02754. CCG. 2 hits.
PF13183. Fer4_8. 1 hit.
[Graphical view]
PIRSFiPIRSF000139. Glc_ox_4Fe-4S. 1 hit.
PROSITEiPS00198. 4FE4S_FER_1. 2 hits.
PS51379. 4FE4S_FER_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "glc locus of Escherichia coli: characterization of genes encoding the subunits of glycolate oxidase and the glc regulator protein."
    Pellicer M.T., Badia J., Aguilar J.T., Baldoma L.
    J. Bacteriol. 178:2051-2059(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. Cited for: SEQUENCE REVISION.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Entry informationi

Entry nameiGLCF_ECOLI
AccessioniPrimary (citable) accession number: P52074
Secondary accession number(s): P76654, Q2M9L8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: January 20, 2016
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.