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Protein

dITP/XTP pyrophosphatase

Gene

ML1175

Organism
Mycobacterium leprae (strain TN)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP. Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions.UniRule annotation

Catalytic activityi

XTP + H2O = XMP + diphosphate.UniRule annotation
dITP + H2O = dIMP + diphosphate.UniRule annotation
ITP + H2O = IMP + diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei76Proton acceptorUniRule annotation1
Metal bindingi76MagnesiumUniRule annotation1
Binding sitei77Substrate; via amide nitrogenUniRule annotation1
Binding sitei184SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processNucleotide metabolism
LigandMagnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
dITP/XTP pyrophosphataseUniRule annotation (EC:3.6.1.66UniRule annotation)
Alternative name(s):
Non-canonical purine NTP pyrophosphataseUniRule annotation
Non-standard purine NTP pyrophosphataseUniRule annotation
Nucleoside-triphosphate diphosphataseUniRule annotation
Nucleoside-triphosphate pyrophosphataseUniRule annotation
Short name:
NTPaseUniRule annotation
Gene namesi
Ordered Locus Names:ML1175
ORF Names:B1549_C2_213, MLCB1701.01
OrganismiMycobacterium leprae (strain TN)
Taxonomic identifieri272631 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000000806 Componenti: Chromosome

Organism-specific databases

LepromaiML1175.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001781931 – 208dITP/XTP pyrophosphataseAdd BLAST208

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi272631.ML1175.

Structurei

3D structure databases

ProteinModelPortaliP52063.
SMRiP52063.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni11 – 16Substrate bindingUniRule annotation6
Regioni158 – 161Substrate bindingUniRule annotation4
Regioni189 – 190Substrate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the HAM1 NTPase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0127. LUCA.
HOGENOMiHOG000293319.
OrthoDBiPOG091H02BP.

Family and domain databases

CDDicd00515. HAM1. 1 hit.
Gene3Di3.90.950.10. 1 hit.
HAMAPiMF_01405. Non_canon_purine_NTPase. 1 hit.
InterProiView protein in InterPro
IPR020922. dITP/XTP_pyrophosphatase.
IPR002637. Ham1p-like.
IPR029001. ITPase-like_fam.
PANTHERiPTHR11067. PTHR11067. 1 hit.
PfamiView protein in Pfam
PF01725. Ham1p_like. 1 hit.
SUPFAMiSSF52972. SSF52972. 1 hit.

Sequencei

Sequence statusi: Complete.

P52063-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALVTKLLVA SRNWKKLAEL RRVLDNAGLS GLTLVSLNDV VPFDEAPEAG
60 70 80 90 100
ATFEDNALAK ARDAFAATGL ASVADDSGLE AAALGGMPGV LSARWSGSYG
110 120 130 140 150
DDAGNTALLL AQLCDVPDER RSAAFVSACA LVSESDEVVV RGVWPGTIAR
160 170 180 190 200
EPRGYGGFGY DSIFIPDGPG LGGRTVAQLR PAEKDAFSHR FRALTLLMPA

LRVLAMRT
Length:208
Mass (Da):21,820
Last modified:August 30, 2002 - v3
Checksum:i9A23FBA4269CE981
GO

Sequence cautioni

The sequence AAA50892 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAB39141 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAC31556 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00014 Genomic DNA. Translation: AAA50892.1. Different initiation.
AL049191 Genomic DNA. Translation: CAB39141.1. Different initiation.
AL583921 Genomic DNA. Translation: CAC31556.1. Different initiation.
PIRiA87056.
S72787.
RefSeqiWP_010908181.1. NC_002677.1.

Genome annotation databases

EnsemblBacteriaiCAC31556; CAC31556; CAC31556.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiIXTPA_MYCLE
AccessioniPrimary (citable) accession number: P52063
Secondary accession number(s): P53428, Q9S383
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: August 30, 2002
Last modified: July 5, 2017
This is version 100 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families