P52046 (CRT_CLOAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-hydroxybutyryl-CoA dehydratase
EC=4.2.1.55

Alternative name(s):
Crotonase

Gene names

Name:	crt
Ordered Locus Names:	CA_C2712

Organism

Clostridium acetobutylicum

Taxonomic identifier

1488 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	261 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(3R)-3-hydroxybutanoyl-CoA = crotonoyl-CoA + H2O.
Pathway	Lipid metabolism; butanoate metabolism.
Sequence similarities	Belongs to the enoyl-CoA hydratase/isomerase family.

Ontologies

Keywords
Biological process	Fatty acid metabolism Lipid metabolism
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	fatty acid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3-hydroxybutyryl-CoA dehydratase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 261	261	3-hydroxybutyryl-CoA dehydratase		PRO_0000109317

Sequences

	Sequence		Length	Mass (Da)
	P52046 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: C9D70D4D2FBF26D4	FASTA	261	28,190
	10 20 30 40 50 60 MELNNVILEK EGKVAVVTIN RPKALNALNS DTLKEMDYVI GEIENDSEVL AVILTGAGEK 70 80 90 100 110 120 SFVAGADISE MKEMNTIEGR KFGILGNKVF RRLELLEKPV IAAVNGFALG GGCEIAMSCD 130 140 150 160 170 180 IRIASSNARF GQPEVGLGIT PGFGGTQRLS RLVGMGMAKQ LIFTAQNIKA DEALRIGLVN 190 200 210 220 230 240 KVVEPSELMN TAKEIANKIV SNAPVAVKLS KQAINRGMQC DIDTALAFES EAFGECFSTE 250 260 DQKDAMTAFI EKRKIEGFKN R

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, sequencing, and expression of clustered genes encoding beta-hydroxybutyryl-coenzyme A (CoA) dehydrogenase, crotonase, and butyryl-CoA dehydrogenase from Clostridium acetobutylicum ATCC 824." Boynton Z.L., Bennett G.N., Rudolph F.B. J. Bacteriol. 178:3015-3024(1996) [PubMed: 8655474] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.
[2]	"Genome sequence and comparative analysis of the solvent-producing bacterium Clostridium acetobutylicum." Noelling J., Breton G., Omelchenko M.V., Makarova K.S., Zeng Q., Gibson R., Lee H.M., Dubois J., Qiu D., Hitti J., Wolf Y.I., Tatusov R.L., Sabathe F., Doucette-Stamm L.A., Soucaille P., Daly M.J., Bennett G.N., Koonin E.V., Smith D.R. J. Bacteriol. 183:4823-4838(2001) [PubMed: 11466286] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U17110 Genomic DNA. Translation: AAA95967.1. AE001437 Genomic DNA. Translation: AAK80658.1.
PIR	G97233. T47261.
RefSeq	NP_349318.1. NC_003030.1.
3D structure databases
ProteinModelPortal	P52046.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1118895.
GenomeReviews	Gene locus CA_C2712 in contig AE001437_GR.
KEGG	cac:CA_C2712.
NMPDR	fig|272562.1.peg.2847.
PATRIC	32039837. VBICloAce74127_2902.
Phylogenomic databases
HOGENOM	HBG748731.
OMA	LGKAKEM.
PhylomeDB	P52046.
ProtClustDB	PRK05809.
Enzyme and pathway databases
BioCyc	CACE272562:CAC2712-MONOMER. MetaCyc:CRTCLOS-MONOMER.
Family and domain databases
InterPro	IPR014748. Crontonase_C. IPR001753. Crotonase_core. IPR018376. Enoyl-CoA_hyd/isom_CS. [Graphical view]
Gene3D	G3DSA:1.10.12.10. Crontonase_C. 1 hit.
KO	K01715.
Pfam	PF00378. ECH. 1 hit. [Graphical view]
PROSITE	PS00166. ENOYL_COA_HYDRATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CRT_CLOAB

Accession

Primary (citable) accession number: P52046

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	January 25, 2012
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program