Reviewed, UniProtKB/Swiss-Prot P52037 (YGFF_ECOLI)

Names and origin

Protein names

Recommended name:
    Uncharacterized oxidoreductase ygfF
    EC=1.-.-.-

Gene names

Name:	ygfF
Synonyms:	yqfD
Ordered Locus Names:	b2902, JW2870

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	247 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Sequence similarities

Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Ontologies

Keywords
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	oxidoreductase activity Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
lpdA	P0A9P0	1	EBI-561028,EBI-542856

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 247	247	Uncharacterized oxidoreductase ygfF		PRO_0000054836
Regions
	Nucleotide binding	4 – 28	25	NAD or NADP By similarity
Sites
	Active site	156	1	Proton acceptor By similarity
	Binding site	142	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P52037-1 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: 8F17257B5905E4F9	FASTA	247	25,938
	10 20 30 40 50 60 MAIALVTGGS RGIGRATALL LAQEGYTVAV NYQQNLHAAQ EVMNLITQAG GKAFVLQADI 70 80 90 100 110 120 SDENQVVAMF TAIDQHDEPL AALVNNAGIL FTQCTVENLT AERINRVLST NVTGYFLCCR 130 140 150 160 170 180 EAVKRMALKN GGSGGAIVNV SSVASRLGSP GEYVDYAASK GAIDTLTTGL SLEVAAQGIR 190 200 210 220 230 240 VNCVRPGFIY TEMHASGGEP GRVDRVKSNI PMQRGGQAEE VAQAIVWLLS DKASYVTGSF IDLAGGK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"Analysis of products of the Escherichia coli genomic genes and regulation of their expressions: an applicable procedure for genomic analysis of other microorganisms." Talukder A.A., Yanai S., Yamada M. Biosci. Biotechnol. Biochem. 58:117-120(1994) [PubMed: 7764507] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-74. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Sequence of minutes 4-25 of Escherichia coli." Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 137-197. Strain: K12 / MG1655 / ATCC 47076.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U28377 Genomic DNA. Translation: AAA69070.1. U28375 Genomic DNA. Translation: AAA83083.1. U00096 Genomic DNA. Translation: AAC75940.1. AP009048 Genomic DNA. Translation: BAE76967.1. D21144 Genomic DNA. Translation: BAA04680.1. U83189 Genomic DNA. Translation: AAB40290.1.
PIR	F65074.
RefSeq	AP_003461.1. NP_417378.1.
3D structure databases
HSSP	HSSP built from PDB template 1FK8 based on UniProtKB Q9ZFY9.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:12165N.
IntAct	P52037. 3 interactions.
Genome annotation databases
GeneID	947373.
GenomeReviews	Gene locus JW2870 in contig AP009048_GR. Gene locus b2902 in contig U00096_GR.
KEGG	ecj:JW2870. eco:b2902.
Organism-specific databases
EchoBASE	EB2798.
EcoGene	EG12971. ygfF.
CMR	Search...
Phylogenomic databases
HOGENOM	P52037.
OMA	P52037. RADISDE.
Enzyme and pathway databases
BioCyc	EcoCyc:G7514-MON.
Family and domain databases
InterPro	IPR002198. DH_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR19410. ADH_short_C2. 1 hit.
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH. PR00080. SDRFAMILY.
PROSITE	PS00061. ADH_SHORT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

YGFF_ECOLI

Accession

Primary (citable) accession number: P52037
Secondary accession number(s): P77138, Q2M9T9, Q46830

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	November 1, 1997
Last modified:	June 16, 2009
This is version 69 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)