P52034 (K6PF_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
6-phosphofructokinase
Short name=Phosphofructokinase
EC=2.7.1.11

Alternative name(s):
Phosphohexokinase

Gene names

Name:	Pfk
ORF Names:	CG4001

Organism

Drosophila melanogaster (Fruit fly) [Reference proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophilina › Drosophiliti › Drosophila › Sophophora › melanogaster group › melanogaster subgroup

Protein attributes

Sequence length	788 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.
Sequence similarities	Belongs to the phosphofructokinase family. Two domains subfamily.
Sequence caution	The sequence AAN71109.1 differs from that shown. Reason: Frameshift at position 218.

Ontologies

Keywords
Biological process	Glycolysis
Coding sequence diversity	Alternative splicing
Domain	Repeat
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Allosteric enzyme Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	fructose 6-phosphate metabolic process Inferred from electronic annotation. Source: InterPro glycolysis Inferred from mutant phenotype Ref.1. Source: FlyBase
Cellular_component	6-phosphofructokinase complex Inferred by curator Ref.1. Source: FlyBase
Molecular_function	6-phosphofructokinase activity Inferred from mutant phenotype Ref.1. Source: FlyBase ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform B (identifier: P52034-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform A (identifier: P52034-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-15: MNSEINQRFLARGSQ → MHSIKFRVFT...IDFVHPVKPF

Note: No experimental confirmation available.

Isoform C (identifier: P52034-3)

The sequence of this isoform differs from the canonical sequence as follows:
     219-250: LVGGLACEADFIFIPEMPPKVDWPDRLCSQLA → ISAAIATEADFMFIPEEPVSVNWKDEICVKLH

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 788	788	6-phosphofructokinase		PRO_0000112031
Regions
	Nucleotide binding	37 – 41	5	ATP By similarity
	Nucleotide binding	212 – 228	17	ATP By similarity
Sites
	Active site	168	1	Proton acceptor By similarity
	Binding site	203	1	Substrate By similarity
	Binding site	294	1	Substrate By similarity
	Binding site	300	1	Substrate By similarity
	Binding site	303	1	Substrate By similarity
Natural variations
	Alternative sequence	1 – 15	15	MNSEI…ARGSQ → MHSIKFRVFTKLKPIFLEIN GRIPICRHFHGPTTFRLEIS NKTPPIRQKLTFPNIGIQCT RSHHLCCPRDISGNTLLSVK FNCKRHCIKLRSDSGDQKND SPGEKNIQKDKSAQRCGKPI NNLHNGFLNAVNYSEKNAVK KKKSAPKRKCGKSVDELRKC LRTMQDVIDFVHPVKPF in isoform A.		VSP_014950
	Alternative sequence	219 – 250	32	LVGGL…CSQLA → ISAAIATEADFMFIPEEPVS VNWKDEICVKLH in isoform C.		VSP_014951
Experimental info
	Sequence conflict	68 – 70	3	QEA → RKS in AAA62385. Ref.1
	Sequence conflict	80 – 92	13	HRGGT…SARCQ → PFVGWHHPLLRPLP Ref.1
	Sequence conflict	130 – 131	2	FR → LP in AAA62385. Ref.1
	Sequence conflict	160 – 161	2	IV → ML in AAA62385. Ref.1
	Sequence conflict	190 – 199	10	AIDAISSTAY → QSKAKVQSPVQPN in AAA62385. Ref.1
	Sequence conflict	209 – 212	4	VMGR → GQVS in AAA62385. Ref.1
	Sequence conflict	228 – 252	25	DFIFI…QLAQE → IHIHPNAPGRLGQTGSALSW TQ in AAA62385. Ref.1
	Sequence conflict	313	1	I → F in AAA62385. Ref.1
	Sequence conflict	322 – 325	4	ATLA → PLWP in AAA62385. Ref.1
	Sequence conflict	360 – 361	2	VA → G in AAA62385. Ref.1
	Sequence conflict	374 – 375	2	KL → NV in AAA62385. Ref.1
	Sequence conflict	455	1	G → R in AAA62385. Ref.1
	Sequence conflict	478	1	T → S in AAA62385. Ref.1
	Sequence conflict	522 – 526	5	DNYPQ → TTTHS in AAA62385. Ref.1
	Sequence conflict	590 – 591	2	AT → PP in AAA62385. Ref.1
	Sequence conflict	613	1	D → E in AAA62385. Ref.1
	Sequence conflict	622 – 636	15	ASKMA…GLILR → PPRWPRRLPRSNPA in AAA62385. Ref.1
	Sequence conflict	695 – 705	11	WLAAQIKANID → CWPPRSRRTST in AAA62385. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform B [UniParc]. Last modified August 16, 2005. Version 2. Checksum: BE45A03013299B75	FASTA	788	86,648
	10 20 30 40 50 60 MNSEINQRFL ARGSQKDKGL AVFTSGGDSQ GMNAAVRACV RMAIYLGCKV YFIREGYQGM 70 80 90 100 110 120 VDGGDCIQEA NWASVSSIIH RGGTIIGSAR CQDFRERQGR LKAANNLIQR GITNLVVIGG 130 140 150 160 170 180 DGSLTGANLF RQEWSSLLDE LVKNKTITTE QQEKFNVLHI VGLVGSIDND FCGTDMTIGT 190 200 210 220 230 240 DTALHRIIEA IDAISSTAYS HQRTFIMEVM GRHCGYLALV GGLACEADFI FIPEMPPKVD 250 260 270 280 290 300 WPDRLCSQLA QERSAGQRLN IVIVAEGAMD REGHPITAED VKKVIDERLK HDARITVLGH 310 320 330 340 350 360 VQRGGNPSAF DRILACRMGA EATLALMEAT KDSVPVVISL DGNQAVRVPL MECVERTQAV 370 380 390 400 410 420 AKAMAEKRWA DAVKLRGRSF ERNLETYKML TRLKPPKENF DADGKGIEGY RLAVMHIGAP 430 440 450 460 470 480 ACGMNAAVRS FVRNAIYRGD VVYGINDGVE GLIAGNVREL GWSDVSGWVG QGGAYLGTKR 490 500 510 520 530 540 TLPEGKFKEI AARLKEFKIQ GLLIIGGFES YHAAGQIADQ RDNYPQFCIP IVVIPSTISN 550 560 570 580 590 600 NVPGTEFSLG CDTGLNEITE ICDRIRQSAQ GTKRRVFVIE TMGGYCGYLA TLAGLAGGAD 610 620 630 640 650 660 AAYIYEEKFS IKDLQQDVYH MASKMAEGVS RGLILRNEKA SENYSTDFIY RLYSEEGKGL 670 680 690 700 710 720 FTCRMNILGH MQQGGSPTPF DRNMGTKMAA KCVDWLAAQI KANIDANGVV NCKSPDTATL 730 740 750 760 770 780 LGIVSRQYRF SPLVDLIAET NFDQRIPKKQ WWLRLRPLLR ILAKHDSAYE EEGMYITVEE ECDTDAVA
	Isoform A [UniParc]. Checksum: 28BD631321945759	FASTA	950	105,187
	10 20 30 40 50 60 MHSIKFRVFT KLKPIFLEIN GRIPICRHFH GPTTFRLEIS NKTPPIRQKL TFPNIGIQCT 70 80 90 100 110 120 RSHHLCCPRD ISGNTLLSVK FNCKRHCIKL RSDSGDQKND SPGEKNIQKD KSAQRCGKPI 130 140 150 160 170 180 NNLHNGFLNA VNYSEKNAVK KKKSAPKRKC GKSVDELRKC LRTMQDVIDF VHPVKPFKDK 190 200 210 220 230 240 GLAVFTSGGD SQGMNAAVRA CVRMAIYLGC KVYFIREGYQ GMVDGGDCIQ EANWASVSSI 250 260 270 280 290 300 IHRGGTIIGS ARCQDFRERQ GRLKAANNLI QRGITNLVVI GGDGSLTGAN LFRQEWSSLL 310 320 330 340 350 360 DELVKNKTIT TEQQEKFNVL HIVGLVGSID NDFCGTDMTI GTDTALHRII EAIDAISSTA 370 380 390 400 410 420 YSHQRTFIME VMGRHCGYLA LVGGLACEAD FIFIPEMPPK VDWPDRLCSQ LAQERSAGQR 430 440 450 460 470 480 LNIVIVAEGA MDREGHPITA EDVKKVIDER LKHDARITVL GHVQRGGNPS AFDRILACRM 490 500 510 520 530 540 GAEATLALME ATKDSVPVVI SLDGNQAVRV PLMECVERTQ AVAKAMAEKR WADAVKLRGR 550 560 570 580 590 600 SFERNLETYK MLTRLKPPKE NFDADGKGIE GYRLAVMHIG APACGMNAAV RSFVRNAIYR 610 620 630 640 650 660 GDVVYGINDG VEGLIAGNVR ELGWSDVSGW VGQGGAYLGT KRTLPEGKFK EIAARLKEFK 670 680 690 700 710 720 IQGLLIIGGF ESYHAAGQIA DQRDNYPQFC IPIVVIPSTI SNNVPGTEFS LGCDTGLNEI 730 740 750 760 770 780 TEICDRIRQS AQGTKRRVFV IETMGGYCGY LATLAGLAGG ADAAYIYEEK FSIKDLQQDV 790 800 810 820 830 840 YHMASKMAEG VSRGLILRNE KASENYSTDF IYRLYSEEGK GLFTCRMNIL GHMQQGGSPT 850 860 870 880 890 900 PFDRNMGTKM AAKCVDWLAA QIKANIDANG VVNCKSPDTA TLLGIVSRQY RFSPLVDLIA 910 920 930 940 950 ETNFDQRIPK KQWWLRLRPL LRILAKHDSA YEEEGMYITV EEECDTDAVA
	Isoform C [UniParc]. Checksum: D4E991EADAD0B084	FASTA	788	86,720
	10 20 30 40 50 60 MNSEINQRFL ARGSQKDKGL AVFTSGGDSQ GMNAAVRACV RMAIYLGCKV YFIREGYQGM 70 80 90 100 110 120 VDGGDCIQEA NWASVSSIIH RGGTIIGSAR CQDFRERQGR LKAANNLIQR GITNLVVIGG 130 140 150 160 170 180 DGSLTGANLF RQEWSSLLDE LVKNKTITTE QQEKFNVLHI VGLVGSIDND FCGTDMTIGT 190 200 210 220 230 240 DTALHRIIEA IDAISSTAYS HQRTFIMEVM GRHCGYLAIS AAIATEADFM FIPEEPVSVN 250 260 270 280 290 300 WKDEICVKLH QERSAGQRLN IVIVAEGAMD REGHPITAED VKKVIDERLK HDARITVLGH 310 320 330 340 350 360 VQRGGNPSAF DRILACRMGA EATLALMEAT KDSVPVVISL DGNQAVRVPL MECVERTQAV 370 380 390 400 410 420 AKAMAEKRWA DAVKLRGRSF ERNLETYKML TRLKPPKENF DADGKGIEGY RLAVMHIGAP 430 440 450 460 470 480 ACGMNAAVRS FVRNAIYRGD VVYGINDGVE GLIAGNVREL GWSDVSGWVG QGGAYLGTKR 490 500 510 520 530 540 TLPEGKFKEI AARLKEFKIQ GLLIIGGFES YHAAGQIADQ RDNYPQFCIP IVVIPSTISN 550 560 570 580 590 600 NVPGTEFSLG CDTGLNEITE ICDRIRQSAQ GTKRRVFVIE TMGGYCGYLA TLAGLAGGAD 610 620 630 640 650 660 AAYIYEEKFS IKDLQQDVYH MASKMAEGVS RGLILRNEKA SENYSTDFIY RLYSEEGKGL 670 680 690 700 710 720 FTCRMNILGH MQQGGSPTPF DRNMGTKMAA KCVDWLAAQI KANIDANGVV NCKSPDTATL 730 740 750 760 770 780 LGIVSRQYRF SPLVDLIAET NFDQRIPKKQ WWLRLRPLLR ILAKHDSAYE EEGMYITVEE ECDTDAVA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure and expression of the gene encoding phosphofructokinase (PFK) in Drosophila melanogaster." Currie P.D., Sullivan D.T. J. Biol. Chem. 269:24679-24687(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM B). Strain: Oregon-R.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING. Strain: Berkeley.
[4]	"A Drosophila complementary DNA resource." Rubin G.M., Hong L., Brokstein P., Evans-Holm M., Frise E., Stapleton M., Harvey D.A. Science 287:2222-2224(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A). Strain: Berkeley. Tissue: Head.
[5]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 101-788 (ISOFORM C). Strain: Berkeley. Tissue: Testis.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L27653 Genomic DNA. Translation: AAA62385.1. AE013599 Genomic DNA. Translation: AAF58840.1. AE013599 Genomic DNA. Translation: AAF58841.1. AE013599 Genomic DNA. Translation: AAM71065.2. AF145673 mRNA. Translation: AAD38648.1. BT001354 mRNA. Translation: AAN71109.1. Frameshift.
PIR	A55034.
RefSeq	NP_523676.1. NM_078952.3. NP_724890.1. NM_165746.3. NP_724891.2. NM_165747.4.
UniGene	Dm.7095.
3D structure databases
ProteinModelPortal	P52034.
SMR	P52034. Positions 18-766.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-928619.
Proteomic databases
PaxDb	P52034.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0088422; FBpp0087508; FBgn0003071.
GeneID	36060.
KEGG	dme:Dmel_CG4001.
UCSC	CG4001-RA. d. melanogaster.
Organism-specific databases
CTD	36060.
FlyBase	FBgn0003071. Pfk.
Phylogenomic databases
eggNOG	COG0205.
GeneTree	ENSGT00390000013209.
HOGENOM	HOG000148827.
InParanoid	P52034.
KO	K00850.
OrthoDB	EOG4PNVXS.
Enzyme and pathway databases
UniPathway	UPA00109; UER00182.
Gene expression databases
Bgee	P52034.
GermOnline	CG4001. Drosophila melanogaster.
Family and domain databases
InterPro	IPR009161. 6-phosphofructokinase_euk. IPR022953. Phosphofructokinase. IPR015912. Phosphofructokinase_CS. IPR000023. Phosphofructokinase_dom. [Graphical view]
Pfam	PF00365. PFK. 2 hits. [Graphical view]
PIRSF	PIRSF000533. ATP_PFK_euk. 1 hit.
PRINTS	PR00476. PHFRCTKINASE.
SUPFAM	SSF53784. Ppfruckinase. 2 hits.
TIGRFAMs	TIGR02478. 6PF1K_euk. 1 hit.
PROSITE	PS00433. PHOSPHOFRUCTOKINASE. 2 hits. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	36060.
NextBio	796655.

Entry information

Entry name

K6PF_DROME

Accession

Primary (citable) accession number: P52034
Secondary accession number(s): Q8IH94 , Q8MKV4, Q9V5G7, Q9Y100

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	August 16, 2005
Last modified:	April 3, 2013
This is version 97 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project