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P52032 (GPX1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Phospholipid hydroperoxide glutathione peroxidase 1, chloroplastic

Short name=PHGPx
EC=1.11.1.12
Gene names
Name:GPX1
Ordered Locus Names:At2g25080
ORF Names:F13D4.40
OrganismArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonscore eudicotyledonsrosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length236 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Protects cells and enzymes from oxidative damage, by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxide, by glutathione By similarity.

Catalytic activity

2 glutathione + a lipid hydroperoxide = glutathione disulfide + lipid + 2 H2O.

Subcellular location

Plastidchloroplast stroma Ref.6.

Tissue specificity

Expressed in leaves, stems, flowers, green siliques and seeds. Ref.7

Induction

By salt stress, osmotic stress, metals and heat treatment. Up-regulated by abscisic acid (ABA) and auxin. Ref.7

Sequence similarities

Belongs to the glutathione peroxidase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 6464Chloroplast Potential
Chain65 – 236172Phospholipid hydroperoxide glutathione peroxidase 1, chloroplastic
PRO_0000013086

Sites

Active site1111 By similarity

Experimental info

Sequence conflict181V → F in CAA61965. Ref.1
Sequence conflict2361A → ELKNF Ref.1

Sequences

Sequence LengthMass (Da)Tools
P52032 [UniParc].

Last modified March 27, 2002. Version 2.
Checksum: D676C0381526C37A

FASTA23626,016
        10         20         30         40         50         60 
MVSMTTSSSS YGTFSTVVNS SRPNSSATFL VPSLKFSTGI SNFANLSNGF SLKSPINPGF 

        70         80         90        100        110        120 
LFKSRPFTVQ ARAAAEKTVH DFTVKDIDGK DVALNKFKGK VMLIVNVASR CGLTSSNYSE 

       130        140        150        160        170        180 
LSHLYEKYKT QGFEILAFPC NQFGFQEPGS NSEIKQFACT RFKAEFPIFD KVDVNGPSTA 

       190        200        210        220        230 
PIYEFLKSNA GGFLGGLIKW NFEKFLIDKK GKVVERYPPT TSPFQIEKDI QKLLAA 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and sequencing of a glutathione peroxidase homologue from Arabidopsis thaliana."
Gachotte D., Benveniste P.
Plant Gene Register PGR95-133
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Columbia.
[2]"Identification of cDNAS encoding plastid-targeted glutathione peroxidase."
Mullineaux P.M., Karpinski S., Jimenez A., Cleary S.P., Robinson C., Creissen G.P.
Plant J. 13:375-379(1998) [PubMed: 9680987] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Columbia.
[3]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed: 10617197] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[4]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[5]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed: 14593172] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[6]"Proteomics of the chloroplast envelope membranes from Arabidopsis thaliana."
Ferro M., Salvi D., Brugiere S., Miras S., Kowalski S., Louwagie M., Garin J., Joyard J., Rolland N.
Mol. Cell. Proteomics 2:325-345(2003) [PubMed: 12766230] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
Strain: cv. Wassilewskija.
[7]"Glutathione peroxidase genes in Arabidopsis are ubiquitous and regulated by abiotic stresses through diverse signaling pathways."
Rodriguez Milla M.A., Maurer A., Rodriguez Huete A., Gustafson J.P.
Plant J. 36:602-615(2003) [PubMed: 14617062] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE, TISSUE SPECIFICITY, INDUCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X89866 mRNA. Translation: CAA61965.1.
AJ000469 mRNA. Translation: CAA04112.1.
CP002685 Genomic DNA. Translation: AEC07655.1.
AY035153 mRNA. Translation: AAK59657.1.
AY063024 mRNA. Translation: AAL34198.1.
IPIIPI00520738.
PIRA84644.
S71250.
RefSeqNP_180080.1. NM_128065.4.
UniGeneAt.24670.

3D structure databases

ProteinModelPortalP52032.
SMRP52032. Positions 77-234.
ModBaseSearch...

Protein-protein interaction databases

STRINGP52032.

Protein family/group databases

PeroxiBase2499. AtGPx01.

Proteomic databases

PRIDEP52032.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT2G25080.1; AT2G25080.1; AT2G25080.
GeneID817046.
GenomeReviewsGene locus AT2G25080 in contig CT485783_GR.
KEGGath:AT2G25080.
NMPDRfig|3702.1.peg.9525.

Organism-specific databases

GeneFarm2047. 163.
TAIRAt2g25080.

Phylogenomic databases

eggNOGKOG1651.
GeneTreeEPGT00050000008661.
HOGENOMHBG648990.
InParanoidP52032.
OMASFCELNF.
PhylomeDBP52032.
ProtClustDBPLN02399.

Gene expression databases

ArrayExpressP52032.
GenevestigatorP52032.
GermOnlineAT2G25080. Arabidopsis thaliana.

Family and domain databases

InterProIPR000889. Glutathione_peroxidase.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
Gene3DG3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
KOK00432.
PANTHERPTHR11592. Glut_peroxidase. 1 hit.
PfamPF00255. GSHPx. 1 hit.
[Graphical view]
PIRSFPIRSF000303. Glutathion_perox. 1 hit.
PRINTSPR01011. GLUTPROXDASE.
SUPFAMSSF52833. Thiordxn-like_fd. 1 hit.
PROSITEPS00460. GLUTATHIONE_PEROXID_1. 1 hit.
PS00763. GLUTATHIONE_PEROXID_2. 1 hit.
PS51355. GLUTATHIONE_PEROXID_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGPX1_ARATH
AccessionPrimary (citable) accession number: P52032
Secondary accession number(s): O19985, O81717
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: March 27, 2002
Last modified: November 16, 2011
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families