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Protein

Glucose-6-phosphate isomerase

Gene

Pgi

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

D-glucose 6-phosphate = D-fructose 6-phosphate.

Pathway:iglycolysis

This protein is involved in step 2 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (Pgi)
  3. ATP-dependent 6-phosphofructokinase (Pfk-RB), ATP-dependent 6-phosphofructokinase (Pfk), ATP-dependent 6-phosphofructokinase (Pfk), ATP-dependent 6-phosphofructokinase (Pfk-RB), ATP-dependent 6-phosphofructokinase (Pfk), ATP-dependent 6-phosphofructokinase (Pfk-RC)
  4. Fructose-bisphosphate aldolase (CG5432-RA), Fructose-bisphosphate aldolase (Ald-RH), Fructose-bisphosphate aldolase (Ald-RH), Fructose-bisphosphate aldolase (CG5432-RA), Fructose-bisphosphate aldolase (Ald-RC), Fructose-bisphosphate aldolase (Ald-RI), Fructose-bisphosphate aldolase (Ald-RI), Fructose-bisphosphate aldolase (Ald-RH), Fructose-bisphosphate aldolase (Ald), Fructose-bisphosphate aldolase (Ald), Fructose-bisphosphate aldolase (Ald)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei362 – 3621Proton donorBy similarity
Active sitei393 – 3931By similarity
Active sitei523 – 5231By similarity

GO - Molecular functioni

  • glucose-6-phosphate isomerase activity Source: FlyBase

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Gluconeogenesis, Glycolysis

Enzyme and pathway databases

ReactomeiREACT_319845. Glycolysis.
REACT_330212. Gluconeogenesis.
REACT_358894. TP53 Regulates Metabolic Genes.
UniPathwayiUPA00109; UER00181.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucose-6-phosphate isomerase (EC:5.3.1.9)
Short name:
GPI
Alternative name(s):
Phosphoglucose isomerase
Short name:
PGI
Phosphohexose isomerase
Short name:
PHI
Gene namesi
Name:Pgi
ORF Names:CG8251
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0003074. Pgi.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • lipid particle Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 558558Glucose-6-phosphate isomerasePRO_0000180543Add
BLAST

Proteomic databases

PaxDbiP52029.
PRIDEiP52029.

Expressioni

Gene expression databases

BgeeiP52029.
GenevisibleiP52029. DM.

Interactioni

Protein-protein interaction databases

BioGridi61725. 4 interactions.
DIPiDIP-17637N.
IntActiP52029. 5 interactions.
MINTiMINT-314135.
STRINGi7227.FBpp0087760.

Structurei

3D structure databases

ProteinModelPortaliP52029.
SMRiP52029. Positions 8-554.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GPI family.Curated

Phylogenomic databases

eggNOGiCOG0166.
GeneTreeiENSGT00390000000707.
InParanoidiP52029.
KOiK01810.
OMAiCETQAML.
OrthoDBiEOG7FXZXV.
PhylomeDBiP52029.

Family and domain databases

Gene3Di1.10.1390.10. 1 hit.
HAMAPiMF_00473. G6P_isomerase.
InterProiIPR001672. G6P_Isomerase.
IPR023096. G6P_Isomerase_C.
IPR018189. Phosphoglucose_isomerase_CS.
[Graphical view]
PANTHERiPTHR11469. PTHR11469. 1 hit.
PfamiPF00342. PGI. 1 hit.
[Graphical view]
PRINTSiPR00662. G6PISOMERASE.
PROSITEiPS00765. P_GLUCOSE_ISOMERASE_1. 1 hit.
PS00174. P_GLUCOSE_ISOMERASE_2. 1 hit.
PS51463. P_GLUCOSE_ISOMERASE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P52029-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGPLPPLNQ EAAFQKLQEY YDSKGKDLNI KDLFVKDSKR FSKYSLRLHT
60 70 80 90 100
QNDGEILLDY SKNRINDEVW DLLLTLAKVR RVNAARDAMF SGQHINITEN
110 120 130 140 150
RAVLHTALRN RGTDPVLVDD KDVMPDVRAE LAHMKEFTNM VISGVWRGCT
160 170 180 190 200
GKQITDVVNI GIGGSDLGPL MVTEALKPYG KGLHSHFVSN IDGTHLAEVL
210 220 230 240 250
KKVNYETTLF IVASKTFTTQ ETITNATSAK TWLLEHSKEP ESVAKHFVAL
260 270 280 290 300
STNKEKVTEF GIDSTNMFGF WDWVGGRYSL WSAIGLSICL SIGFENFEQL
310 320 330 340 350
LDGAHFMDNH FKTTPFEKNA PVILALLGVW YSNFFKAETH ALLPYDQYLH
360 370 380 390 400
RFAAYFQQGD MESNGKFVSK SGKPVKYSTG PIVWGEPGTN GQHAFYQLIH
410 420 430 440 450
QGTRLIPCDF IAPAQTHNPI AGGKHHKILL SNFLAQTEAL MAGKTVDEAR
460 470 480 490 500
TELSKAGLCG NELDNLLPHK VFVGNRPTNS IVVKKVSPFT LGALIALYEH
510 520 530 540 550
KIFVQGIIWD INSFDQWGVE LGKQLAKAIE PELDHCNEVS THDSSTNGLI

NFIKANWK
Length:558
Mass (Da):62,339
Last modified:June 1, 2001 - v2
Checksum:i8DD54FCFCCBD1488
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti184 – 1841H → Y in strain: LW15. 1 Publication
Natural varianti329 – 3291V → I in strain: AF-F, F1-F, F1-1S, JA-F, LW8, MW18 and ZW20. 1 Publication
Natural varianti471 – 4711V → A in strain: JA-S. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L27539 Genomic DNA. Translation: AAA28789.1.
L27540 Genomic DNA. Translation: AAA28790.1.
L27541 Genomic DNA. Translation: AAA28791.1.
L27542 Genomic DNA. Translation: AAA28792.1.
L27543 Genomic DNA. Translation: AAA28793.1.
L27544 Genomic DNA. Translation: AAA28794.1.
L27545 Genomic DNA. Translation: AAA28795.1.
L27546 Genomic DNA. Translation: AAA28796.1.
L27553 Genomic DNA. Translation: AAA28803.1.
L27554 Genomic DNA. Translation: AAA28804.1.
L27555 Genomic DNA. Translation: AAA28805.1.
U20566 Genomic DNA. Translation: AAA63671.1.
U20567 Genomic DNA. Translation: AAA63672.1.
U20568 Genomic DNA. Translation: AAA63673.1.
U20569 Genomic DNA. Translation: AAA63674.1.
U20570 Genomic DNA. Translation: AAA63675.1.
U20571 Genomic DNA. Translation: AAA63676.1.
U20572 Genomic DNA. Translation: AAA63677.1.
U20573 Genomic DNA. Translation: AAA63678.1.
U20574 Genomic DNA. Translation: AAA63679.1.
U20575 Genomic DNA. Translation: AAA63680.1.
AE013599 Genomic DNA. Translation: AAF59025.1.
AE013599 Genomic DNA. Translation: AAM71091.1.
AY051468 mRNA. Translation: AAK92892.1.
RefSeqiNP_523663.1. NM_078939.3.
NP_724723.1. NM_165635.2.
NP_724724.1. NM_165636.2.
UniGeneiDm.2587.

Genome annotation databases

EnsemblMetazoaiFBtr0088679; FBpp0087760; FBgn0003074.
FBtr0088680; FBpp0087761; FBgn0003074.
FBtr0088681; FBpp0087762; FBgn0003074.
GeneIDi35886.
KEGGidme:Dmel_CG8251.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L27539 Genomic DNA. Translation: AAA28789.1.
L27540 Genomic DNA. Translation: AAA28790.1.
L27541 Genomic DNA. Translation: AAA28791.1.
L27542 Genomic DNA. Translation: AAA28792.1.
L27543 Genomic DNA. Translation: AAA28793.1.
L27544 Genomic DNA. Translation: AAA28794.1.
L27545 Genomic DNA. Translation: AAA28795.1.
L27546 Genomic DNA. Translation: AAA28796.1.
L27553 Genomic DNA. Translation: AAA28803.1.
L27554 Genomic DNA. Translation: AAA28804.1.
L27555 Genomic DNA. Translation: AAA28805.1.
U20566 Genomic DNA. Translation: AAA63671.1.
U20567 Genomic DNA. Translation: AAA63672.1.
U20568 Genomic DNA. Translation: AAA63673.1.
U20569 Genomic DNA. Translation: AAA63674.1.
U20570 Genomic DNA. Translation: AAA63675.1.
U20571 Genomic DNA. Translation: AAA63676.1.
U20572 Genomic DNA. Translation: AAA63677.1.
U20573 Genomic DNA. Translation: AAA63678.1.
U20574 Genomic DNA. Translation: AAA63679.1.
U20575 Genomic DNA. Translation: AAA63680.1.
AE013599 Genomic DNA. Translation: AAF59025.1.
AE013599 Genomic DNA. Translation: AAM71091.1.
AY051468 mRNA. Translation: AAK92892.1.
RefSeqiNP_523663.1. NM_078939.3.
NP_724723.1. NM_165635.2.
NP_724724.1. NM_165636.2.
UniGeneiDm.2587.

3D structure databases

ProteinModelPortaliP52029.
SMRiP52029. Positions 8-554.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi61725. 4 interactions.
DIPiDIP-17637N.
IntActiP52029. 5 interactions.
MINTiMINT-314135.
STRINGi7227.FBpp0087760.

Proteomic databases

PaxDbiP52029.
PRIDEiP52029.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0088679; FBpp0087760; FBgn0003074.
FBtr0088680; FBpp0087761; FBgn0003074.
FBtr0088681; FBpp0087762; FBgn0003074.
GeneIDi35886.
KEGGidme:Dmel_CG8251.

Organism-specific databases

CTDi35886.
FlyBaseiFBgn0003074. Pgi.

Phylogenomic databases

eggNOGiCOG0166.
GeneTreeiENSGT00390000000707.
InParanoidiP52029.
KOiK01810.
OMAiCETQAML.
OrthoDBiEOG7FXZXV.
PhylomeDBiP52029.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00181.
ReactomeiREACT_319845. Glycolysis.
REACT_330212. Gluconeogenesis.
REACT_358894. TP53 Regulates Metabolic Genes.

Miscellaneous databases

GenomeRNAii35886.
NextBioi795651.
PROiP52029.

Gene expression databases

BgeeiP52029.
GenevisibleiP52029. DM.

Family and domain databases

Gene3Di1.10.1390.10. 1 hit.
HAMAPiMF_00473. G6P_isomerase.
InterProiIPR001672. G6P_Isomerase.
IPR023096. G6P_Isomerase_C.
IPR018189. Phosphoglucose_isomerase_CS.
[Graphical view]
PANTHERiPTHR11469. PTHR11469. 1 hit.
PfamiPF00342. PGI. 1 hit.
[Graphical view]
PRINTSiPR00662. G6PISOMERASE.
PROSITEiPS00765. P_GLUCOSE_ISOMERASE_1. 1 hit.
PS00174. P_GLUCOSE_ISOMERASE_2. 1 hit.
PS51463. P_GLUCOSE_ISOMERASE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. McDonald J.H., Kreitman M.E.
    Submitted (JAN-1994) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS TYR-184; ILE-329 AND ALA-471.
    Strain: AF-F, AF-S, F1-1S, F1-F, FL-2S, FR-F, FR-S, JA-F, JA-S, LW15, LW8, MW13, MW17, MW18, WA-F, WA-S, ZW20, ZW29, ZW34, ZW53 and ZW56.
  2. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  3. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Berkeley.
    Tissue: Head.

Entry informationi

Entry nameiG6PI_DROME
AccessioniPrimary (citable) accession number: P52029
Secondary accession number(s): A4UZ93
, Q0E9F0, Q27868, Q27870, Q9V4Z8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: June 1, 2001
Last modified: June 24, 2015
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.