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Protein

Squalene monooxygenase

Gene

Sqle

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the first oxygenation step in sterol biosynthesis and is suggested to be one of the rate-limiting enzymes in this pathway.

Catalytic activityi

Squalene + [reduced NADPH--hemoprotein reductase] + O2 = (3S)-2,3-epoxy-2,3-dihydrosqualene + [oxidized NADPH--hemoprotein reductase] + H2O.

Cofactori

Pathwayi: lanosterol biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes lanosterol from farnesyl diphosphate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Squalene synthase (Fdft1)
  2. Squalene monooxygenase (Sqle)
  3. Lanosterol synthase (Lss)
This subpathway is part of the pathway lanosterol biosynthesis, which is itself part of Terpene metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes lanosterol from farnesyl diphosphate, the pathway lanosterol biosynthesis and in Terpene metabolism.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi125 – 15228FADSequence analysisAdd
BLAST

GO - Molecular functioni

  • flavin adenine dinucleotide binding Source: InterPro
  • squalene monooxygenase activity Source: RGD

GO - Biological processi

  • cellular aromatic compound metabolic process Source: RGD
  • cholesterol metabolic process Source: RGD
  • response to organic substance Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein, NADP

Enzyme and pathway databases

ReactomeiR-RNO-191273. Cholesterol biosynthesis.
R-RNO-2426168. Activation of gene expression by SREBF (SREBP).
SABIO-RKP52020.
UniPathwayiUPA00767; UER00752.

Names & Taxonomyi

Protein namesi
Recommended name:
Squalene monooxygenase (EC:1.14.14.17)
Alternative name(s):
Squalene epoxidase
Short name:
SE
Gene namesi
Name:Sqle
Synonyms:Erg1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 7

Organism-specific databases

RGDi3755. Sqle.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei20 – 4021HelicalSequence analysisAdd
BLAST
Transmembranei60 – 8021HelicalSequence analysisAdd
BLAST
Transmembranei118 – 13821HelicalSequence analysisAdd
BLAST
Transmembranei545 – 56521HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL4241.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 573573Squalene monooxygenasePRO_0000209840Add
BLAST

Proteomic databases

PaxDbiP52020.
PRIDEiP52020.

Expressioni

Gene expression databases

GenevisibleiP52020. RN.

Interactioni

Subunit structurei

May form a complex with squalene synthase.

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000012883.

Chemistry

BindingDBiP52020.

Structurei

3D structure databases

ProteinModelPortaliP52020.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the squalene monooxygenase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1298. Eukaryota.
COG0654. LUCA.
GeneTreeiENSGT00390000011759.
HOGENOMiHOG000174713.
HOVERGENiHBG005603.
InParanoidiP52020.
KOiK00511.
OMAiAYYRAPR.
OrthoDBiEOG7J446G.
PhylomeDBiP52020.
TreeFamiTF331056.

Family and domain databases

Gene3Di3.50.50.60. 2 hits.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR013698. Squalene_epoxidase.
[Graphical view]
PfamiPF08491. SE. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 2 hits.

Sequencei

Sequence statusi: Complete.

P52020-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWTFLGIATF TYFYKKCGDV TLANKELLLC VLVFLSLGLV LSYRCRHRNG
60 70 80 90 100
GLLGRHQSGS QFAAFSDILS ALPLIGFFWA KSPPESEKKE QLESKRRRKE
110 120 130 140 150
VNLSETTLTG AATSVSTSSV TDPEVIIIGS GVLGSALATV LSRDGRTVTV
160 170 180 190 200
IERDLKEPDR ILGECLQPGG YRVLRELGLG DTVESLNAHH IHGYVIHDCE
210 220 230 240 250
SRSEVQIPYP VSENNQVQSG VAFHHGKFIM SLRKAAMAEP NVKFIEGVVL
260 270 280 290 300
RLLEEDDAVI GVQYKDKETG DTKELHAPLT VVADGLFSKF RKNLISNKVS
310 320 330 340 350
VSSHFVGFIM KDAPQFKANF AELVLVDPSP VLIYQISPSE TRVLVDIRGE
360 370 380 390 400
LPRNLREYMT EQIYPQIPDH LKESFLEACQ NARLRTMPAS FLPPSSVNKR
410 420 430 440 450
GVLLLGDAYN LRHPLTGGGM TVALKDIKIW RQLLKDIPDL YDDAAIFQAK
460 470 480 490 500
KSFFWSRKRS HSFVVNVLAQ ALYELFSATD DSLRQLRKAC FLYFKLGGEC
510 520 530 540 550
LTGPVGLLSI LSPDPLLLIR HFFSVAVYAT YFCFKSEPWA TKPRALFSSG
560 570
AILYKACSII FPLIYSEMKY LVH
Length:573
Mass (Da):64,024
Last modified:October 1, 1996 - v1
Checksum:i55222C911E2B8777
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D37920 mRNA. Translation: BAA07141.1.
BC097330 mRNA. Translation: AAH97330.1.
PIRiA55767.
RefSeqiNP_058832.1. NM_017136.2.
UniGeneiRn.33239.

Genome annotation databases

EnsembliENSRNOT00000012883; ENSRNOP00000012883; ENSRNOG00000009550.
GeneIDi29230.
KEGGirno:29230.
UCSCiRGD:3755. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D37920 mRNA. Translation: BAA07141.1.
BC097330 mRNA. Translation: AAH97330.1.
PIRiA55767.
RefSeqiNP_058832.1. NM_017136.2.
UniGeneiRn.33239.

3D structure databases

ProteinModelPortaliP52020.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000012883.

Chemistry

BindingDBiP52020.
ChEMBLiCHEMBL4241.

Proteomic databases

PaxDbiP52020.
PRIDEiP52020.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000012883; ENSRNOP00000012883; ENSRNOG00000009550.
GeneIDi29230.
KEGGirno:29230.
UCSCiRGD:3755. rat.

Organism-specific databases

CTDi6713.
RGDi3755. Sqle.

Phylogenomic databases

eggNOGiKOG1298. Eukaryota.
COG0654. LUCA.
GeneTreeiENSGT00390000011759.
HOGENOMiHOG000174713.
HOVERGENiHBG005603.
InParanoidiP52020.
KOiK00511.
OMAiAYYRAPR.
OrthoDBiEOG7J446G.
PhylomeDBiP52020.
TreeFamiTF331056.

Enzyme and pathway databases

UniPathwayiUPA00767; UER00752.
ReactomeiR-RNO-191273. Cholesterol biosynthesis.
R-RNO-2426168. Activation of gene expression by SREBF (SREBP).
SABIO-RKP52020.

Miscellaneous databases

PROiP52020.

Gene expression databases

GenevisibleiP52020. RN.

Family and domain databases

Gene3Di3.50.50.60. 2 hits.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR013698. Squalene_epoxidase.
[Graphical view]
PfamiPF08491. SE. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 2 hits.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and expression of rat squalene epoxidase."
    Sakakibara J., Watanabe R., Kanai Y., Ono T.
    J. Biol. Chem. 270:17-20(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Kidney.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Placenta.

Entry informationi

Entry nameiERG1_RAT
AccessioniPrimary (citable) accession number: P52020
Secondary accession number(s): Q4V8L3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 8, 2016
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.