P52020 (ERG1_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Squalene monooxygenase
EC=1.14.13.132

Alternative name(s):
Squalene epoxidase
Short name=SE

Gene names

Name:	Sqle
Synonyms:	Erg1

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	573 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the first oxygenation step in sterol biosynthesis and is suggested to be one of the rate-limiting enzymes in this pathway.
Catalytic activity	Squalene + NADPH + O2 = (3S)-2,3-epoxy-2,3-dihydrosqualene + NADP+ + H2O.
Cofactor	FAD.
Pathway	Terpene metabolism; lanosterol biosynthesis; lanosterol from farnesyl diphosphate: step 2/3.
Subunit structure	May form a complex with squalene synthase.
Subcellular location	Microsome membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the squalene monooxygenase family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Domain	Transmembrane Transmembrane helix
Ligand	FAD Flavoprotein NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cellular aromatic compound metabolic process Inferred from direct assay Ref.1. Source: RGD cholesterol metabolic process Inferred from mutant phenotype. Source: RGD response to organic substance Inferred from direct assay. Source: RGD
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW microsome Inferred from direct assay. Source: RGD
Molecular function	flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro squalene monooxygenase activity Inferred from direct assay Ref.1. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 573	573	Squalene monooxygenase		PRO_0000209840
Regions
	Transmembrane	20 – 40	21	Helical; Potential
	Transmembrane	60 – 80	21	Helical; Potential
	Transmembrane	118 – 138	21	Helical; Potential
	Transmembrane	545 – 565	21	Helical; Potential
	Nucleotide binding	125 – 152	28	FAD Potential

Sequences

	Sequence		Length	Mass (Da)
	P52020 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 55222C911E2B8777	FASTA	573	64,024
	10 20 30 40 50 60 MWTFLGIATF TYFYKKCGDV TLANKELLLC VLVFLSLGLV LSYRCRHRNG GLLGRHQSGS 70 80 90 100 110 120 QFAAFSDILS ALPLIGFFWA KSPPESEKKE QLESKRRRKE VNLSETTLTG AATSVSTSSV 130 140 150 160 170 180 TDPEVIIIGS GVLGSALATV LSRDGRTVTV IERDLKEPDR ILGECLQPGG YRVLRELGLG 190 200 210 220 230 240 DTVESLNAHH IHGYVIHDCE SRSEVQIPYP VSENNQVQSG VAFHHGKFIM SLRKAAMAEP 250 260 270 280 290 300 NVKFIEGVVL RLLEEDDAVI GVQYKDKETG DTKELHAPLT VVADGLFSKF RKNLISNKVS 310 320 330 340 350 360 VSSHFVGFIM KDAPQFKANF AELVLVDPSP VLIYQISPSE TRVLVDIRGE LPRNLREYMT 370 380 390 400 410 420 EQIYPQIPDH LKESFLEACQ NARLRTMPAS FLPPSSVNKR GVLLLGDAYN LRHPLTGGGM 430 440 450 460 470 480 TVALKDIKIW RQLLKDIPDL YDDAAIFQAK KSFFWSRKRS HSFVVNVLAQ ALYELFSATD 490 500 510 520 530 540 DSLRQLRKAC FLYFKLGGEC LTGPVGLLSI LSPDPLLLIR HFFSVAVYAT YFCFKSEPWA 550 560 570 TKPRALFSSG AILYKACSII FPLIYSEMKY LVH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and expression of rat squalene epoxidase." Sakakibara J., Watanabe R., Kanai Y., Ono T. J. Biol. Chem. 270:17-20(1995) [PubMed: 7814369] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Kidney.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Placenta.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D37920 mRNA. Translation: BAA07141.1. BC097330 mRNA. Translation: AAH97330.1.
IPI	IPI00202028.
PIR	A55767.
RefSeq	NP_058832.1. NM_017136.2.
UniGene	Rn.33239.
3D structure databases
ProteinModelPortal	P52020.
ModBase	Search...
Protein-protein interaction databases
STRING	P52020.
Proteomic databases
PRIDE	P52020.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000012883; ENSRNOP00000012883; ENSRNOG00000009550.
GeneID	29230.
KEGG	rno:29230.
UCSC	NM_017136. rat.
Organism-specific databases
CTD	6713.
RGD	3755. Sqle.
Phylogenomic databases
eggNOG	roNOG07673.
HOVERGEN	HBG005603.
InParanoid	P52020.
OMA	NEMREAC.
OrthoDB	EOG4NZTT2.
PhylomeDB	P52020.
Gene expression databases
ArrayExpress	P52020.
Genevestigator	P52020.
GermOnline	ENSRNOG00000009550. Rattus norvegicus.
Family and domain databases
InterPro	IPR003042. Rng_hydrolase-like. IPR013698. Squalene_epoxidase. [Graphical view]
KO	K00511.
Pfam	PF08491. SE. 1 hit. [Graphical view]
PRINTS	PR00420. RNGMNOXGNASE.
ProtoNet	Search...
Other
NextBio	608474.

Entry information

Entry name

ERG1_RAT

Accession

Primary (citable) accession number: P52020
Secondary accession number(s): Q4V8L3

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	January 25, 2012
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program