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Protein

Squalene monooxygenase

Gene

Sqle

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the first oxygenation step in sterol biosynthesis and is suggested to be one of the rate-limiting enzymes in this pathway.

Catalytic activityi

Squalene + NADPH + O2 = (3S)-2,3-epoxy-2,3-dihydrosqualene + NADP+ + H2O.

Cofactori

Pathway: lanosterol biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes lanosterol from farnesyl diphosphate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Squalene synthase (Fdft1)
  2. Squalene monooxygenase (Sqle)
  3. Lanosterol synthase (Lss)
This subpathway is part of the pathway lanosterol biosynthesis, which is itself part of Terpene metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes lanosterol from farnesyl diphosphate, the pathway lanosterol biosynthesis and in Terpene metabolism.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi124 – 15128FADSequence AnalysisAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein, NADP

Enzyme and pathway databases

ReactomeiREACT_303828. Activation of gene expression by SREBF (SREBP).
REACT_345006. Cholesterol biosynthesis.
UniPathwayiUPA00767; UER00752.

Names & Taxonomyi

Protein namesi
Recommended name:
Squalene monooxygenase (EC:1.14.13.132)
Alternative name(s):
Squalene epoxidase
Short name:
SE
Gene namesi
Name:Sqle
Synonyms:Erg1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:109296. Sqle.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei20 – 4021HelicalSequence AnalysisAdd
BLAST
Transmembranei60 – 8021HelicalSequence AnalysisAdd
BLAST
Transmembranei118 – 13821HelicalSequence AnalysisAdd
BLAST
Transmembranei544 – 56421HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 572572Squalene monooxygenasePRO_0000209839Add
BLAST

Proteomic databases

MaxQBiP52019.
PaxDbiP52019.
PRIDEiP52019.

PTM databases

PhosphoSiteiP52019.

Expressioni

Gene expression databases

BgeeiP52019.
CleanExiMM_SQLE.
ExpressionAtlasiP52019. baseline and differential.
GenevisibleiP52019. MM.

Interactioni

Subunit structurei

May form a complex with squalene synthase.

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000022977.

Structurei

3D structure databases

ProteinModelPortaliP52019.
SMRiP52019. Positions 122-154.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the squalene monooxygenase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0654.
HOGENOMiHOG000174713.
HOVERGENiHBG005603.
InParanoidiP52019.
KOiK00511.
OMAiLRKACFF.
OrthoDBiEOG7J446G.
PhylomeDBiP52019.
TreeFamiTF331056.

Family and domain databases

InterProiIPR013698. Squalene_epoxidase.
[Graphical view]
PfamiPF08491. SE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P52019-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWTFLGIATF TYFYKKCGDV TLANKELLLC VLVFLSLGLV LSYRCRHRHG
60 70 80 90 100
GLLGRHQSGA QFAAFSDILS ALPLIGFFWA KSPESEKKEQ LESKKCRKEI
110 120 130 140 150
GLSETTLTGA ATSVSTSFVT DPEVIIVGSG VLGSALAAVL SRDGRKVTVI
160 170 180 190 200
ERDLKEPDRI VGELLQPGGY RVLQELGLGD TVEGLNAHHI HGYIVHDYES
210 220 230 240 250
RSEVQIPYPL SETNQVQSGI AFHHGRFIMS LRKAAMAEPN VKFIEGVVLQ
260 270 280 290 300
LLEEDDAVIG VQYKDKETGD TKELHAPLTV VADGLFSKFR KSLISSKVSV
310 320 330 340 350
SSHFVGFLMK DAPQFKPNFA ELVLVNPSPV LIYQISSSET RVLVDIRGEL
360 370 380 390 400
PRNLREYMAE QIYPQLPEHL KESFLEASQN GRLRTMPASF LPPSSVNKRG
410 420 430 440 450
VLILGDAYNL RHPLTGGGMT VALKDIKLWR QLLKDIPDLY DDAAIFQAKK
460 470 480 490 500
SFFWSRKRTH SFVVNVLAQA LYELFSATDD SLHQLRKACF LYFKLGGECV
510 520 530 540 550
TGPVGLLSIL SPHPLVLIRH FFSVAIYATY FCFKSEPWAT KPRALFSSGA
560 570
VLYKACSILF PLIYSEMKYL VH
Length:572
Mass (Da):63,770
Last modified:October 1, 1996 - v1
Checksum:i5C30D80A204664D4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D42048 mRNA. Translation: BAA07649.1.
BC042781 mRNA. Translation: AAH42781.1.
BC056361 mRNA. Translation: AAH56361.1.
CCDSiCCDS27497.1.
PIRiS52113.
RefSeqiNP_033296.1. NM_009270.3.
UniGeneiMm.296169.

Genome annotation databases

EnsembliENSMUST00000022977; ENSMUSP00000022977; ENSMUSG00000022351.
GeneIDi20775.
KEGGimmu:20775.
UCSCiuc007vxq.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D42048 mRNA. Translation: BAA07649.1.
BC042781 mRNA. Translation: AAH42781.1.
BC056361 mRNA. Translation: AAH56361.1.
CCDSiCCDS27497.1.
PIRiS52113.
RefSeqiNP_033296.1. NM_009270.3.
UniGeneiMm.296169.

3D structure databases

ProteinModelPortaliP52019.
SMRiP52019. Positions 122-154.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000022977.

PTM databases

PhosphoSiteiP52019.

Proteomic databases

MaxQBiP52019.
PaxDbiP52019.
PRIDEiP52019.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000022977; ENSMUSP00000022977; ENSMUSG00000022351.
GeneIDi20775.
KEGGimmu:20775.
UCSCiuc007vxq.1. mouse.

Organism-specific databases

CTDi6713.
MGIiMGI:109296. Sqle.

Phylogenomic databases

eggNOGiCOG0654.
HOGENOMiHOG000174713.
HOVERGENiHBG005603.
InParanoidiP52019.
KOiK00511.
OMAiLRKACFF.
OrthoDBiEOG7J446G.
PhylomeDBiP52019.
TreeFamiTF331056.

Enzyme and pathway databases

UniPathwayiUPA00767; UER00752.
ReactomeiREACT_303828. Activation of gene expression by SREBF (SREBP).
REACT_345006. Cholesterol biosynthesis.

Miscellaneous databases

ChiTaRSiSqle. mouse.
NextBioi299491.
PROiP52019.
SOURCEiSearch...

Gene expression databases

BgeeiP52019.
CleanExiMM_SQLE.
ExpressionAtlasiP52019. baseline and differential.
GenevisibleiP52019. MM.

Family and domain databases

InterProiIPR013698. Squalene_epoxidase.
[Graphical view]
PfamiPF08491. SE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of a cDNA for mouse squalene epoxidase."
    Kosuga K., Hata S., Osumi T., Sakakibara J., Ono T.
    Biochim. Biophys. Acta 1260:345-348(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6 X CBA.
    Tissue: Liver.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6 and NMRI.
    Tissue: Brain and Mammary gland.

Entry informationi

Entry nameiERG1_MOUSE
AccessioniPrimary (citable) accession number: P52019
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 24, 2015
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.