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Protein

Nucleoside triphosphatase NudI

Gene

nudI

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the hydrolysis of nucleoside triphosphates, with a preference for pyrimidine deoxynucleoside triphosphates (dUTP, dTTP and dCTP).1 Publication

Catalytic activityi

A nucleoside triphosphate + H2O = a nucleotide + diphosphate.1 Publication
dUTP + H2O = dUMP + diphosphate.1 Publication
dTTP + H2O = dTMP + diphosphate.1 Publication
dCTP + H2O = dCMP + diphosphate.1 Publication

Cofactori

Mg2+1 Publication

Kineticsi

kcat is 21 sec(-1) with dUTP as substrate, and 15 sec(-1) with dTTP as substrate.1 Publication
  1. KM=2.2 mM for dUTP1 Publication
  2. KM=1.3 mM for dTTP1 Publication
  1. Vmax=81 µmol/min/mg enzyme with dUTP as substrate1 Publication
  2. Vmax=56 µmol/min/mg enzyme with dTTP as substrate1 Publication

pH dependencei

Optimum pH is about 8.5.1 Publication

GO - Molecular functioni

  • dCTP diphosphatase activity Source: EcoCyc
  • dUTP diphosphatase activity Source: EcoCyc
  • NADH pyrophosphatase activity Source: UniProtKB-EC

Keywordsi

Molecular functionHydrolase
LigandMagnesium

Enzyme and pathway databases

BioCyciEcoCyc:G7164-MONOMER
MetaCyc:G7164-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleoside triphosphatase NudI1 Publication (EC:3.6.1.91 Publication)
Alternative name(s):
Nucleotide diphosphatase NudIUniRule annotation
Pyrimidine deoxynucleoside triphosphate diphosphataseCurated
dCTP diphosphatase (EC:3.6.1.121 Publication)
dTTP diphosphatase (EC:3.6.1.-1 Publication)
dUTP diphosphatase (EC:3.6.1.231 Publication)
Gene namesi
Name:nudI
Synonyms:yfaO
Ordered Locus Names:b2251, JW2245
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13275 nudI

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000571391 – 141Nucleoside triphosphatase NudIAdd BLAST141

Proteomic databases

PaxDbiP52006
PRIDEiP52006

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

BioGridi4261215, 13 interactors
IntActiP52006, 7 interactors
STRINGi316385.ECDH10B_2411

Structurei

3D structure databases

ProteinModelPortaliP52006
SMRiP52006
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 141Nudix hydrolaseAdd BLAST141

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi38 – 59Nudix boxAdd BLAST22

Sequence similaritiesi

Belongs to the Nudix hydrolase family. NudI subfamily.Curated

Phylogenomic databases

eggNOGiENOG4108RJ7 Bacteria
COG0494 LUCA
HOGENOMiHOG000059287
InParanoidiP52006
KOiK12944
OMAiRTTKWRG

Family and domain databases

HAMAPiMF_01846 Nudix_NudI, 1 hit
InterProiView protein in InterPro
IPR023781 Nucleoside_triphosphatase_NudI
IPR020476 Nudix_hydrolase
IPR015797 NUDIX_hydrolase-like_dom_sf
IPR020084 NUDIX_hydrolase_CS
IPR000086 NUDIX_hydrolase_dom
PfamiView protein in Pfam
PF00293 NUDIX, 1 hit
PRINTSiPR00502 NUDIXFAMILY
SUPFAMiSSF55811 SSF55811, 1 hit
PROSITEiView protein in PROSITE
PS51462 NUDIX, 1 hit
PS00893 NUDIX_BOX, 1 hit

Sequencei

Sequence statusi: Complete.

P52006-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRQRTIVCPL IQNDGAYLLC KMADDRGVFP GQWAISGGGV EPGERIEEAL
60 70 80 90 100
RREIREELGE QLLLTEITPW TFSDDIRTKT YADGRKEEIY MIYLIFDCVS
110 120 130 140
ANREVKINEE FQDYAWVKPE DLVHYDLNVA TRKTLRLKGL L
Length:141
Mass (Da):16,371
Last modified:November 1, 1997 - v2
Checksum:i6AD2A51D276F13AB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC75311.1
AP009048 Genomic DNA Translation: BAA16074.1
X83874 Genomic DNA No translation available.
PIRiA64996
RefSeqiNP_416754.1, NC_000913.3
WP_001300564.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75311; AAC75311; b2251
BAA16074; BAA16074; BAA16074
GeneIDi946740
KEGGiecj:JW2245
eco:b2251
PATRICifig|511145.12.peg.2343

Similar proteinsi

Entry informationi

Entry nameiNUDI_ECOLI
AccessioniPrimary (citable) accession number: P52006
Secondary accession number(s): P77305
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: November 1, 1997
Last modified: March 28, 2018
This is version 121 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health