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Protein

Nucleoside triphosphatase NudI

Gene

nudI

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the hydrolysis of nucleoside triphosphates, with a preference for pyrimidine deoxynucleoside triphosphates (dUTP, dTTP and dCTP).1 Publication

Catalytic activityi

A nucleoside triphosphate + H2O = a nucleotide + diphosphate.1 Publication
dUTP + H2O = dUMP + diphosphate.1 Publication
dTTP + H2O = dTMP + diphosphate.1 Publication
dCTP + H2O = dCMP + diphosphate.1 Publication

Cofactori

Mg2+1 Publication

Kineticsi

kcat is 21 sec(-1) with dUTP as substrate, and 15 sec(-1) with dTTP as substrate.1 Publication

  1. KM=2.2 mM for dUTP1 Publication
  2. KM=1.3 mM for dTTP1 Publication
  1. Vmax=81 µmol/min/mg enzyme with dUTP as substrate1 Publication
  2. Vmax=56 µmol/min/mg enzyme with dTTP as substrate1 Publication

pH dependencei

Optimum pH is about 8.5.1 Publication

GO - Molecular functioni

  • dCTP diphosphatase activity Source: EcoCyc
  • dUTP diphosphatase activity Source: EcoCyc
  • magnesium ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BioCyciEcoCyc:G7164-MONOMER.
ECOL316407:JW2245-MONOMER.
MetaCyc:G7164-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleoside triphosphatase NudI1 Publication (EC:3.6.1.191 Publication)
Alternative name(s):
Pyrimidine deoxynucleoside triphosphate diphosphataseCurated
dCTP diphosphatase (EC:3.6.1.121 Publication)
dTTP diphosphatase (EC:3.6.1.-1 Publication)
dUTP diphosphatase (EC:3.6.1.231 Publication)
Gene namesi
Name:nudI
Synonyms:yfaO
Ordered Locus Names:b2251, JW2245
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13275. nudI.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 141141Nucleoside triphosphatase NudIPRO_0000057139Add
BLAST

Proteomic databases

PaxDbiP52006.

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

BioGridi4261215. 13 interactions.
IntActiP52006. 7 interactions.
MINTiMINT-1262923.
STRINGi511145.b2251.

Structurei

3D structure databases

ProteinModelPortaliP52006.
SMRiP52006. Positions 1-141.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 141141Nudix hydrolaseAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi38 – 5922Nudix boxAdd
BLAST

Sequence similaritiesi

Belongs to the Nudix hydrolase family. NudI subfamily.Curated
Contains 1 nudix hydrolase domain.Curated

Phylogenomic databases

eggNOGiENOG4108RJ7. Bacteria.
COG0494. LUCA.
HOGENOMiHOG000059287.
InParanoidiP52006.
KOiK12944.
OMAiNEEFDDY.

Family and domain databases

Gene3Di3.90.79.10. 1 hit.
HAMAPiMF_01846. Nudix_NudI. 1 hit.
InterProiIPR023781. Nucleoside_triphosphatase_NudI.
IPR020476. Nudix_hydrolase.
IPR020084. NUDIX_hydrolase_CS.
IPR000086. NUDIX_hydrolase_dom.
IPR015797. NUDIX_hydrolase_dom-like.
[Graphical view]
PfamiPF00293. NUDIX. 1 hit.
[Graphical view]
PRINTSiPR00502. NUDIXFAMILY.
SUPFAMiSSF55811. SSF55811. 1 hit.
PROSITEiPS51462. NUDIX. 1 hit.
PS00893. NUDIX_BOX. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P52006-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRQRTIVCPL IQNDGAYLLC KMADDRGVFP GQWAISGGGV EPGERIEEAL
60 70 80 90 100
RREIREELGE QLLLTEITPW TFSDDIRTKT YADGRKEEIY MIYLIFDCVS
110 120 130 140
ANREVKINEE FQDYAWVKPE DLVHYDLNVA TRKTLRLKGL L
Length:141
Mass (Da):16,371
Last modified:November 1, 1997 - v2
Checksum:i6AD2A51D276F13AB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC75311.1.
AP009048 Genomic DNA. Translation: BAA16074.1.
X83874 Genomic DNA. No translation available.
PIRiA64996.
RefSeqiNP_416754.1. NC_000913.3.
WP_001300564.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75311; AAC75311; b2251.
BAA16074; BAA16074; BAA16074.
GeneIDi946740.
KEGGiecj:JW2245.
eco:b2251.
PATRICi32119867. VBIEscCol129921_2343.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC75311.1.
AP009048 Genomic DNA. Translation: BAA16074.1.
X83874 Genomic DNA. No translation available.
PIRiA64996.
RefSeqiNP_416754.1. NC_000913.3.
WP_001300564.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP52006.
SMRiP52006. Positions 1-141.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261215. 13 interactions.
IntActiP52006. 7 interactions.
MINTiMINT-1262923.
STRINGi511145.b2251.

Proteomic databases

PaxDbiP52006.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75311; AAC75311; b2251.
BAA16074; BAA16074; BAA16074.
GeneIDi946740.
KEGGiecj:JW2245.
eco:b2251.
PATRICi32119867. VBIEscCol129921_2343.

Organism-specific databases

EchoBASEiEB3060.
EcoGeneiEG13275. nudI.

Phylogenomic databases

eggNOGiENOG4108RJ7. Bacteria.
COG0494. LUCA.
HOGENOMiHOG000059287.
InParanoidiP52006.
KOiK12944.
OMAiNEEFDDY.

Enzyme and pathway databases

BioCyciEcoCyc:G7164-MONOMER.
ECOL316407:JW2245-MONOMER.
MetaCyc:G7164-MONOMER.

Miscellaneous databases

PROiP52006.

Family and domain databases

Gene3Di3.90.79.10. 1 hit.
HAMAPiMF_01846. Nudix_NudI. 1 hit.
InterProiIPR023781. Nucleoside_triphosphatase_NudI.
IPR020476. Nudix_hydrolase.
IPR020084. NUDIX_hydrolase_CS.
IPR000086. NUDIX_hydrolase_dom.
IPR015797. NUDIX_hydrolase_dom-like.
[Graphical view]
PfamiPF00293. NUDIX. 1 hit.
[Graphical view]
PRINTSiPR00502. NUDIXFAMILY.
SUPFAMiSSF55811. SSF55811. 1 hit.
PROSITEiPS51462. NUDIX. 1 hit.
PS00893. NUDIX_BOX. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNUDI_ECOLI
AccessioniPrimary (citable) accession number: P52006
Secondary accession number(s): P77305
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: November 1, 1997
Last modified: September 7, 2016
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.