P51998 (RL4P_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
54S ribosomal protein YmL6, mitochondrial

Gene names

Name:	YML6
Ordered Locus Names:	YML025C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	286 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Component of the mitochondrial large ribosomal subunit. Mature mitochondrial ribosomes consist of a small (37S) and a large (54S) subunit. The 37S subunit contains at least 33 different proteins and 1 molecule of RNA (15S). The 54S subunit contains at least 45 different proteins and 1 molecule of RNA (21S). Ref.3 Ref.4
Subcellular location	Mitochondrion Ref.5 Ref.7.
Miscellaneous	Present with 1200 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Belongs to the ribosomal protein L4P family.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Molecular function	Ribonucleoprotein Ribosomal protein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	mitochondrial translation Inferred by curator Ref.4. Source: SGD
Cellular component	mitochondrial large ribosomal subunit Inferred from direct assay Ref.4. Source: SGD
Molecular function	structural constituent of ribosome Inferred from direct assay Ref.4. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 26	26	Mitochondrion Ref.3
	Chain	27 – 286	260	54S ribosomal protein YmL6, mitochondrial		PRO_0000030541
Experimental info
	Sequence conflict	30	1	N → S AA sequence Ref.3
	Sequence conflict	35	1	A → I AA sequence Ref.3
	Sequence conflict	53	1	E → P AA sequence Ref.3
	Sequence conflict	121	1	S → K AA sequence Ref.4
	Sequence conflict	156	1	L → F AA sequence Ref.4
	Sequence conflict	249	1	E → C AA sequence Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P51998 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 50528B571B0411F2	FASTA	286	31,970
	10 20 30 40 50 60 MTIKRNLVKT LQSIRYQATT ATAHAESTLN PLPNAAIPPK YALVTVRSFP SLEPLTFVPV 70 80 90 100 110 120 PTSTVAAPLR RDILWRAVVY ENDNRRVGAS NPPGRSENGF SRRKLMPQKG SGRARVGDAN 130 140 150 160 170 180 SPTRHNGGRA LARTAPNDYT TELPSKVYSM AFNNALSHQY KSGKLFVIGG EKVDLISPTP 190 200 210 220 230 240 ELDLNRLDLV NTNTVEGKEI FEGEVIFRKF LEEFQLKGKR LLFITDKTRE GLIKSSDPYK 250 260 270 280 QKVDVIQKEL VEVNDILRAQ AVFIELEALE YLAMAHQKEI LHSVSN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Extended N-terminal sequencing of proteins of the large ribosomal subunit from yeast mitochondria." Grohmann L., Graack H.-R., Kruft V., Choli T., Goldschmidt-Reisin S., Kitakawa M. FEBS Lett. 284:51-56(1991) [PubMed: 2060626] [Abstract] Cited for: PROTEIN SEQUENCE OF 27-53, SUBUNIT. Strain: 07173.
[4]	"Identification and characterization of the genes for mitochondrial ribosomal proteins of Saccharomyces cerevisiae." Kitakawa M., Graack H.-R., Grohmann L., Goldschmidt-Reisin S., Herfurth E., Wittmann-Liebold B., Nishimura T., Isono K. Eur. J. Biochem. 245:449-456(1997) [PubMed: 9151978] [Abstract] Cited for: PROTEIN SEQUENCE OF 110-121; 147-156; 241-249 AND 279-286, SUBUNIT. Strain: 07173.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"The proteome of Saccharomyces cerevisiae mitochondria." Sickmann A., Reinders J., Wagner Y., Joppich C., Zahedi R.P., Meyer H.E., Schoenfisch B., Perschil I., Chacinska A., Guiard B., Rehling P., Pfanner N., Meisinger C. Proc. Natl. Acad. Sci. U.S.A. 100:13207-13212(2003) [PubMed: 14576278] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. Strain: ATCC 76625 / YPH499.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z46659 Genomic DNA. Translation: CAA86630.1. BK006946 Genomic DNA. Translation: DAA09873.1.
PIR	S50887.
RefSeq	NP_013687.1. NM_001182383.1.
3D structure databases
ProteinModelPortal	P51998.
SMR	P51998. Positions 42-278.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6670N.
IntAct	P51998. 39 interactions.
MINT	MINT-659020.
STRING	P51998.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YML025C; YML025C; YML025C.
GeneID	854983.
KEGG	sce:YML025C.
NMPDR	fig|4932.3.peg.4728.
Organism-specific databases
CYGD	YML025c.
SGD	S000004487. YML6.
Phylogenomic databases
eggNOG	fuNOG04143.
GeneTree	EFGT00050000005243.
HOGENOM	HBG397831.
OMA	TASTKWR.
OrthoDB	EOG41G6DC.
Gene expression databases
ArrayExpress	P51998.
Genevestigator	P51998.
GermOnline	YML025C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR002136. Ribosomal_L4/L1e. IPR013005. Ribosomal_L4/L1e_bac-type. IPR023574. Ribosomal_L4_dom. [Graphical view]
Gene3D	G3DSA:3.40.1370.10. G3DSA:3.40.1370.10. 1 hit.
PANTHER	PTHR10746. Ribos_L4_L1E_bac. 1 hit.
Pfam	PF00573. Ribosomal_L4. 1 hit. [Graphical view]
SUPFAM	SSF52166. Ribosomal_L4/L1E. 1 hit.
ProtoNet	Search...
Other
NextBio	978106.

Entry information

Entry name

RL4P_YEAST

Accession

Primary (citable) accession number: P51998
Secondary accession number(s): D6VZE9

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	December 14, 2011
This is version 93 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program