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Protein

GTP-binding protein YPT32/YPT11

Gene

YPT32

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for protein transport through the secretory pathway. Probably involved in regulation of secretory vesicle formation at the trans-Golgi compartment. Mediates the proper polarized localization of SEC2, a GEF for SEC4, but does not alter the exchange activity of SEC2 on SEC4. Plays a role in autophagy.5 Publications

Enzyme regulationi

Alternates between an inactive form bound to GDP and an active form bound to GTP. Activated by the guanine nucleotide-exchange factor (GEF) TRAPP complex, and inactivated by GTPase-activating protein (GAP) GYP3.2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi20 – 278GTPBy similarity
Nucleotide bindingi68 – 725GTPBy similarity
Nucleotide bindingi126 – 1294GTPBy similarity

GO - Molecular functioni

  • GTPase activity Source: SGD
  • GTP binding Source: UniProtKB-KW

GO - Biological processi

  • autophagy Source: UniProtKB-KW
  • early endosome to Golgi transport Source: SGD
  • exocytosis Source: SGD
  • protein transport Source: UniProtKB-KW
  • small GTPase mediated signal transduction Source: InterPro
  • vesicle-mediated transport Source: SGD
Complete GO annotation...

Keywords - Biological processi

Autophagy, Exocytosis, Protein transport, Transport

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30687-MONOMER.
ReactomeiR-SCE-1445148. Translocation of GLUT4 to the plasma membrane.
R-SCE-6811440. Retrograde transport at the Trans-Golgi-Network.

Names & Taxonomyi

Protein namesi
Recommended name:
GTP-binding protein YPT32/YPT11
Alternative name(s):
Rab GTPase YPT32
Gene namesi
Name:YPT32
Synonyms:YPT11
Ordered Locus Names:YGL210W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGL210W.
SGDiS000003178. YPT32.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: GOC
  • endosome Source: SGD
  • Golgi apparatus Source: SGD
  • Golgi membrane Source: UniProtKB-SubCell
  • recycling endosome Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi27 – 271S → N: Binds preferentially GDP. 1 Publication
Mutagenesisi49 – 491E → Q: Cold sensitive. 1 Publication
Mutagenesisi72 – 721Q → L: Reduces GTPase activity. 1 Publication
Mutagenesisi126 – 1261N → I: Blocks nucleotide binding. 1 Publication
Mutagenesisi141 – 1411A → D: Loss of function at 37 degrees Celsius. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 222221GTP-binding protein YPT32/YPT11PRO_0000121324Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity
Lipidationi221 – 2211S-geranylgeranyl cysteineBy similarity
Lipidationi222 – 2221S-geranylgeranyl cysteineBy similarity

Keywords - PTMi

Acetylation, Lipoprotein, Prenylation

Proteomic databases

MaxQBiP51996.
PRIDEiP51996.

PTM databases

iPTMnetiP51996.

Interactioni

Subunit structurei

Interacts with YIF1, YIP3, YIP4 and YIP5. Interacts with the GEF SEC2. Interacts with TRS130.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
YIF1P538452EBI-29384,EBI-28230
YIP3P536332EBI-29384,EBI-25301
YIP4P530932EBI-29384,EBI-24124

Protein-protein interaction databases

BioGridi33046. 69 interactions.
DIPiDIP-6597N.
IntActiP51996. 6 interactions.
MINTiMINT-703976.

Structurei

Secondary structure

1
222
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi11 – 199Combined sources
Helixi26 – 3510Combined sources
Beta strandi47 – 5711Combined sources
Beta strandi60 – 7112Combined sources
Helixi76 – 838Combined sources
Beta strandi88 – 947Combined sources
Helixi98 – 1025Combined sources
Helixi104 – 11411Combined sources
Beta strandi120 – 1267Combined sources
Helixi128 – 1336Combined sources
Helixi138 – 14710Combined sources
Beta strandi151 – 1544Combined sources
Turni157 – 1593Combined sources
Helixi163 – 17715Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3RWMX-ray2.00B7-188[»]
3RWOX-ray1.70A/B7-188[»]
ProteinModelPortaliP51996.
SMRiP51996. Positions 7-178.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi42 – 509Effector regionCurated

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rab family.Curated

Phylogenomic databases

GeneTreeiENSGT00760000118841.
HOGENOMiHOG000233968.
InParanoidiP51996.
KOiK07905.
OMAiANSTACK.
OrthoDBiEOG092C5MDH.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P51996-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNEDYGYDY DYLFKIVLIG DSGVGKSNLL SRFTTDEFNI ESKSTIGVEF
60 70 80 90 100
ATRTIEVENK KIKAQIWDTA GQERYRAITS AYYRGAVGAL IVYDISKSSS
110 120 130 140 150
YENCNHWLTE LRENADDNVA VGLIGNKSDL AHLRAVPTDE AKNFAMENQM
160 170 180 190 200
LFTETSALNS DNVDKAFREL IVAIFQMVSK HQVDLSGSGT NNMGSNGAPK
210 220
GPTISLTPAP KEDKKKKSSN CC
Length:222
Mass (Da):24,520
Last modified:January 23, 2007 - v3
Checksum:i621217A296161964
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X72834 Genomic DNA. Translation: CAA51355.1.
U33754 Genomic DNA. Translation: AAC49495.1.
Z72732 Genomic DNA. Translation: CAA96926.1.
AY558506 Genomic DNA. Translation: AAS56832.1.
BK006941 Genomic DNA. Translation: DAA07906.1.
PIRiS58514.
RefSeqiNP_011305.1. NM_001181075.1.

Genome annotation databases

EnsemblFungiiYGL210W; YGL210W; YGL210W.
GeneIDi852662.
KEGGisce:YGL210W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X72834 Genomic DNA. Translation: CAA51355.1.
U33754 Genomic DNA. Translation: AAC49495.1.
Z72732 Genomic DNA. Translation: CAA96926.1.
AY558506 Genomic DNA. Translation: AAS56832.1.
BK006941 Genomic DNA. Translation: DAA07906.1.
PIRiS58514.
RefSeqiNP_011305.1. NM_001181075.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3RWMX-ray2.00B7-188[»]
3RWOX-ray1.70A/B7-188[»]
ProteinModelPortaliP51996.
SMRiP51996. Positions 7-178.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33046. 69 interactions.
DIPiDIP-6597N.
IntActiP51996. 6 interactions.
MINTiMINT-703976.

PTM databases

iPTMnetiP51996.

Proteomic databases

MaxQBiP51996.
PRIDEiP51996.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGL210W; YGL210W; YGL210W.
GeneIDi852662.
KEGGisce:YGL210W.

Organism-specific databases

EuPathDBiFungiDB:YGL210W.
SGDiS000003178. YPT32.

Phylogenomic databases

GeneTreeiENSGT00760000118841.
HOGENOMiHOG000233968.
InParanoidiP51996.
KOiK07905.
OMAiANSTACK.
OrthoDBiEOG092C5MDH.

Enzyme and pathway databases

BioCyciYEAST:G3O-30687-MONOMER.
ReactomeiR-SCE-1445148. Translocation of GLUT4 to the plasma membrane.
R-SCE-6811440. Retrograde transport at the Trans-Golgi-Network.

Miscellaneous databases

PROiP51996.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiYPT32_YEAST
AccessioniPrimary (citable) accession number: P51996
Secondary accession number(s): D6VTU5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: January 23, 2007
Last modified: September 7, 2016
This is version 152 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 4298 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.