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Protein

GTP-binding protein YPT32/YPT11

Gene

YPT32

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for protein transport through the secretory pathway. Probably involved in regulation of secretory vesicle formation at the trans-Golgi compartment. Mediates the proper polarized localization of SEC2, a GEF for SEC4, but does not alter the exchange activity of SEC2 on SEC4. Plays a role in autophagy.5 Publications

Miscellaneous

Present with 4298 molecules/cell in log phase SD medium.1 Publication

Enzyme regulationi

Alternates between an inactive form bound to GDP and an active form bound to GTP. Activated by the guanine nucleotide-exchange factor (GEF) TRAPP complex, and inactivated by GTPase-activating protein (GAP) GYP3.2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi20 – 27GTPBy similarity8
Nucleotide bindingi68 – 72GTPBy similarity5
Nucleotide bindingi126 – 129GTPBy similarity4

GO - Molecular functioni

  • GTPase activity Source: SGD
  • GTP binding Source: UniProtKB-KW

GO - Biological processi

  • autophagy Source: UniProtKB-KW
  • early endosome to Golgi transport Source: SGD
  • exocytosis Source: SGD
  • protein transport Source: UniProtKB-KW
  • vesicle-mediated transport Source: SGD

Keywordsi

Biological processAutophagy, Exocytosis, Protein transport, Transport
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30687-MONOMER
ReactomeiR-SCE-6798695 Neutrophil degranulation
R-SCE-8854214 TBC/RABGAPs
R-SCE-8873719 RAB geranylgeranylation

Names & Taxonomyi

Protein namesi
Recommended name:
GTP-binding protein YPT32/YPT11
Alternative name(s):
Rab GTPase YPT32
Gene namesi
Name:YPT32
Synonyms:YPT11
Ordered Locus Names:YGL210W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGL210W
SGDiS000003178 YPT32

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi27S → N: Binds preferentially GDP. 1 Publication1
Mutagenesisi49E → Q: Cold sensitive. 1 Publication1
Mutagenesisi72Q → L: Reduces GTPase activity. 1 Publication1
Mutagenesisi126N → I: Blocks nucleotide binding. 1 Publication1
Mutagenesisi141A → D: Loss of function at 37 degrees Celsius. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001213242 – 222GTP-binding protein YPT32/YPT11Add BLAST221

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Lipidationi221S-geranylgeranyl cysteineBy similarity1
Lipidationi222S-geranylgeranyl cysteineBy similarity1

Keywords - PTMi

Acetylation, Lipoprotein, Prenylation

Proteomic databases

MaxQBiP51996
PaxDbiP51996
PRIDEiP51996

PTM databases

iPTMnetiP51996

Interactioni

Subunit structurei

Interacts with YIF1, YIP3, YIP4 and YIP5. Interacts with the GEF SEC2. Interacts with TRS130.4 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi33046, 106 interactors
DIPiDIP-6597N
IntActiP51996, 7 interactors
MINTiP51996
STRINGi4932.YGL210W

Structurei

Secondary structure

1222
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi11 – 19Combined sources9
Helixi26 – 35Combined sources10
Beta strandi47 – 57Combined sources11
Beta strandi60 – 71Combined sources12
Helixi76 – 83Combined sources8
Beta strandi88 – 94Combined sources7
Helixi98 – 102Combined sources5
Helixi104 – 114Combined sources11
Beta strandi120 – 126Combined sources7
Helixi128 – 133Combined sources6
Helixi138 – 147Combined sources10
Beta strandi151 – 154Combined sources4
Turni157 – 159Combined sources3
Helixi163 – 177Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3RWMX-ray2.00B7-188[»]
3RWOX-ray1.70A/B7-188[»]
ProteinModelPortaliP51996
SMRiP51996
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi42 – 50Effector regionCurated9

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rab family.Curated

Phylogenomic databases

GeneTreeiENSGT00760000118841
HOGENOMiHOG000233968
InParanoidiP51996
KOiK07905
OMAiHENADKK
OrthoDBiEOG092C5MDH

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR001806 Small_GTPase
PfamiView protein in Pfam
PF00071 Ras, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51419 RAB, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P51996-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNEDYGYDY DYLFKIVLIG DSGVGKSNLL SRFTTDEFNI ESKSTIGVEF
60 70 80 90 100
ATRTIEVENK KIKAQIWDTA GQERYRAITS AYYRGAVGAL IVYDISKSSS
110 120 130 140 150
YENCNHWLTE LRENADDNVA VGLIGNKSDL AHLRAVPTDE AKNFAMENQM
160 170 180 190 200
LFTETSALNS DNVDKAFREL IVAIFQMVSK HQVDLSGSGT NNMGSNGAPK
210 220
GPTISLTPAP KEDKKKKSSN CC
Length:222
Mass (Da):24,520
Last modified:January 23, 2007 - v3
Checksum:i621217A296161964
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X72834 Genomic DNA Translation: CAA51355.1
U33754 Genomic DNA Translation: AAC49495.1
Z72732 Genomic DNA Translation: CAA96926.1
AY558506 Genomic DNA Translation: AAS56832.1
BK006941 Genomic DNA Translation: DAA07906.1
PIRiS58514
RefSeqiNP_011305.1, NM_001181075.1

Genome annotation databases

EnsemblFungiiYGL210W; YGL210W; YGL210W
GeneIDi852662
KEGGisce:YGL210W

Similar proteinsi

Entry informationi

Entry nameiYPT32_YEAST
AccessioniPrimary (citable) accession number: P51996
Secondary accession number(s): D6VTU5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: January 23, 2007
Last modified: June 20, 2018
This is version 168 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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