GTP-binding protein YPT32/YPT11 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P51996 (YPT32_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 115. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
GTP-binding protein YPT32/YPT11

Alternative name(s):
Rab GTPase YPT32

Gene names

Name:	YPT32
Synonyms:	YPT11
Ordered Locus Names:	YGL210W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	222 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for protein transport through the secretory pathway. Probably involved in regulation of secretory vesicle formation at the trans-Golgi compartment. Mediates the proper polarized localization of SEC2, a GEF for SEC4, but does not alter the exchange activity of SEC2 on SEC4. Ref.1 Ref.7 Ref.12
Enzyme regulation	Alternates between an inactive form bound to GDP and an active form bound to GTP. Activated by the guanine nucleotide-exchange factor (GEF) TRAPP complex, and inactivated by GTPase-activating protein (GAP) GYP3. Ref.8 Ref.9
Subunit structure	Interacts with YIF1, YIP3, YIP4 and YIP5. Interacts with the GEF SEC2. Ref.10 Ref.11 Ref.12
Subcellular location	Golgi apparatus membrane; Lipid-anchor Ref.7.
Miscellaneous	Present with 4298 molecules/cell in log phase SD medium. Ref.13
Sequence similarities	Belongs to the small GTPase superfamily. Rab family.

Ontologies

Keywords
Biological process	Exocytosis Protein transport Transport
Cellular component	Golgi apparatus Membrane
Ligand	GTP-binding Nucleotide-binding
PTM	Acetylation Lipoprotein Prenylation
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	early endosome to Golgi transport Inferred from genetic interaction. Source: SGD exocytosis Inferred from mutant phenotype Ref.7. Source: SGD protein transport Inferred from electronic annotation. Source: UniProtKB-KW small GTPase mediated signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell endosome Inferred from direct assay. Source: SGD mitochondrial outer membrane Inferred from direct assay. Source: SGD
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Inferred from direct assay. Source: SGD protein binding Inferred from physical interaction Ref.10 Ref.11. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct
GDI1	P39958	4	EBI-29384,EBI-7517
YIF1	P53845	2	EBI-29384,EBI-28230
YIP3	P53633	2	EBI-29384,EBI-25301
YIP4	P53093	2	EBI-29384,EBI-24124

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

Removed By similarity

Chain

2 – 222

221

GTP-binding protein YPT32/YPT11

PRO_0000121324

Regions

Nucleotide binding

20 – 27

GTP By similarity

Nucleotide binding

68 – 72

GTP By similarity

Nucleotide binding

126 – 129

GTP By similarity

Motif

42 – 50

Effector region Probable

Amino acid modifications

Modified residue

N-acetylserine By similarity

Lipidation

221

S-geranylgeranyl cysteine By similarity

Lipidation

222

S-geranylgeranyl cysteine By similarity

Experimental info

Mutagenesis

S → N: Binds preferentially GDP. Ref.12

Mutagenesis

E → Q: Cold sensitive. Ref.12

Mutagenesis

Q → L: Reduces GTPase activity. Ref.12

Mutagenesis

126

N → I: Blocks nucleotide binding. Ref.12

Mutagenesis

141

A → D: Loss of function at 37 degrees Celsius. Ref.7

Sequences

Sequence

Length

Mass (Da)

Tools

P51996 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 621217A296161964
Show »

FASTA

222

24,520

        10         20         30         40         50         60 
MSNEDYGYDY DYLFKIVLIG DSGVGKSNLL SRFTTDEFNI ESKSTIGVEF ATRTIEVENK 

        70         80         90        100        110        120 
KIKAQIWDTA GQERYRAITS AYYRGAVGAL IVYDISKSSS YENCNHWLTE LRENADDNVA 

       130        140        150        160        170        180 
VGLIGNKSDL AHLRAVPTDE AKNFAMENQM LFTETSALNS DNVDKAFREL IVAIFQMVSK 

       190        200        210        220 
HQVDLSGSGT NNMGSNGAPK GPTISLTPAP KEDKKKKSSN CC

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Two GTPase isoforms, Ypt31p and Ypt32p, are essential for Golgi function in yeast." Benli M., Doering F., Robinson D.G., Yang X., Gallwitz D. EMBO J. 15:6460-6475(1996) [PubMed: 8978673] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION. Strain: DUR.
[2]	"Lambda clone B22 contains a 7676 bp genomic fragment of Saccharomyces cerevisiae chromosome VII spanning the VAM7-SPM2 intergenic region and containing three novel transcribed open reading frames." Kail M., Juettner E., Vaux D. Yeast 12:799-807(1996) [PubMed: 8813766] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Analysis of 21.7 kb DNA sequence from the left arm of chromosome VII reveals 11 open reading frames: two correspond to new genes." Feuermann M., Simeonava L., Souciet J.-L., Potier S. Yeast 13:475-477(1997) [PubMed: 9153757] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. Kleine K. Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[5]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[6]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[7]	"Two new Ypt GTPases are required for exit from the yeast trans-Golgi compartment." Jedd G., Mulholland J., Segev N. J. Cell Biol. 137:563-580(1997) [PubMed: 9151665] [Abstract] Cited for: FUNCTION, MUTAGENESIS OF ALA-141, SUBCELLULAR LOCATION.
[8]	"Two new members of a family of Ypt/Rab GTPase activating proteins. Promiscuity of substrate recognition." Albert S., Gallwitz D. J. Biol. Chem. 274:33186-33189(1999) [PubMed: 10559187] [Abstract] Cited for: ENZYME REGULATION.
[9]	"The TRAPP complex is a nucleotide exchanger for Ypt1 and Ypt31/32." Jones S., Newman C., Liu F., Segev N. Mol. Biol. Cell 11:4403-4411(2000) [PubMed: 11102533] [Abstract] Cited for: ENZYME REGULATION.
[10]	"Saccharomyces cerevisiae Pra1p/Yip3p interacts with Yip1p and Rab proteins." Calero M., Collins R.N. Biochem. Biophys. Res. Commun. 290:676-681(2002) [PubMed: 11785952] [Abstract] Cited for: INTERACTION WITH YIP3.
[11]	"Identification of the novel proteins Yip4p and Yip5p as Rab GTPase interacting factors." Calero M., Winand N.J., Collins R.N. FEBS Lett. 515:89-98(2002) [PubMed: 11943201] [Abstract] Cited for: INTERACTION WITH YIF1; YIP4 AND YIP5.
[12]	"Ypt32 recruits the Sec4p guanine nucleotide exchange factor, Sec2p, to secretory vesicles; evidence for a Rab cascade in yeast." Ortiz D., Medkova M., Walch-Solimena C., Novick P.J. J. Cell Biol. 157:1005-1015(2002) [PubMed: 12045183] [Abstract] Cited for: FUNCTION, INTERACTION WITH SEC2, MUTAGENESIS OF SER-27; GLU-49; GLN-72 AND ASN-126.
[13]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X72834 Genomic DNA. Translation: CAA51355.1.
U33754 Genomic DNA. Translation: AAC49495.1.
Z72732 Genomic DNA. Translation: CAA96926.1.
AY558506 Genomic DNA. Translation: AAS56832.1.
BK006941 Genomic DNA. Translation: DAA07906.1.

PIR

S58514.

RefSeq

NP_011305.1. NM_001181075.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3RWM	X-ray	2.00	B	7-188	[»]
3RWO	X-ray	1.70	A/B	7-188	[»]

ProteinModelPortal

P51996.

SMR

P51996. Positions 10-178.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-6597N.

IntAct

P51996. 8 interactions.

MINT

MINT-703976.

STRING

P51996.

Proteomic databases

PeptideAtlas

P51996.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YGL210W; YGL210W; YGL210W.

GeneID

852662.

KEGG

sce:YGL210W.

NMPDR

fig|4932.3.peg.2408.

Organism-specific databases

SGD

S000003178. YPT32.

Phylogenomic databases

eggNOG

fuNOG06551.

GeneTree

EFGT00050000002458.

HOGENOM

HBG745225.

OMA

KGFAERN.

OrthoDB

EOG41K2ND.

Gene expression databases

ArrayExpress

P51996.

Genevestigator

P51996.

GermOnline

YGL210W. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003579. Small_GTPase_Rab_type.
[Graphical view]

K07976.

Pfam

PF00071. Ras. 1 hit.
[Graphical view]

PRINTS

PR00449. RASTRNSFRMNG.

SMART

SM00175. RAB. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00231. Small_GTP. 1 hit.

PROSITE

PS51419. RAB. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

971948.

Entry information

Entry name

YPT32_YEAST

Accession

Primary (citable) accession number: P51996
Secondary accession number(s): D6VTU5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	January 23, 2007
Last modified:	December 14, 2011
This is version 115 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents