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P51996

- YPT32_YEAST

UniProt

P51996 - YPT32_YEAST

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Protein

GTP-binding protein YPT32/YPT11

Gene

YPT32

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Required for protein transport through the secretory pathway. Probably involved in regulation of secretory vesicle formation at the trans-Golgi compartment. Mediates the proper polarized localization of SEC2, a GEF for SEC4, but does not alter the exchange activity of SEC2 on SEC4. Plays a role in autophagy.5 Publications

Enzyme regulationi

Alternates between an inactive form bound to GDP and an active form bound to GTP. Activated by the guanine nucleotide-exchange factor (GEF) TRAPP complex, and inactivated by GTPase-activating protein (GAP) GYP3.2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi20 – 278GTPBy similarity
Nucleotide bindingi68 – 725GTPBy similarity
Nucleotide bindingi126 – 1294GTPBy similarity

GO - Molecular functioni

  1. GTPase activity Source: SGD
  2. GTP binding Source: UniProtKB-KW

GO - Biological processi

  1. autophagy Source: UniProtKB-KW
  2. early endosome to Golgi transport Source: SGD
  3. exocytosis Source: SGD
  4. GTP catabolic process Source: GOC
  5. protein transport Source: UniProtKB-KW
  6. small GTPase mediated signal transduction Source: InterPro
  7. vesicle-mediated transport Source: SGD
Complete GO annotation...

Keywords - Biological processi

Autophagy, Exocytosis, Protein transport, Transport

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30687-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
GTP-binding protein YPT32/YPT11
Alternative name(s):
Rab GTPase YPT32
Gene namesi
Name:YPT32
Synonyms:YPT11
Ordered Locus Names:YGL210W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VII

Organism-specific databases

SGDiS000003178. YPT32.

Subcellular locationi

Golgi apparatus membrane 1 Publication; Lipid-anchor 1 Publication

GO - Cellular componenti

  1. endosome Source: SGD
  2. Golgi apparatus Source: SGD
  3. membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi27 – 271S → N: Binds preferentially GDP. 1 Publication
Mutagenesisi49 – 491E → Q: Cold sensitive. 1 Publication
Mutagenesisi72 – 721Q → L: Reduces GTPase activity. 1 Publication
Mutagenesisi126 – 1261N → I: Blocks nucleotide binding. 1 Publication
Mutagenesisi141 – 1411A → D: Loss of function at 37 degrees Celsius. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 222222GTP-binding protein YPT32/YPT11PRO_0000121324Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi221 – 2211S-geranylgeranyl cysteineBy similarity
Lipidationi222 – 2221S-geranylgeranyl cysteineBy similarity

Keywords - PTMi

Lipoprotein, Prenylation

Proteomic databases

MaxQBiP51996.
PaxDbiP51996.
PeptideAtlasiP51996.
PRIDEiP51996.

Expressioni

Gene expression databases

GenevestigatoriP51996.

Interactioni

Subunit structurei

Interacts with YIF1, YIP3, YIP4 and YIP5. Interacts with the GEF SEC2. Interacts with TRS130.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
YIF1P538452EBI-29384,EBI-28230
YIP3P536332EBI-29384,EBI-25301
YIP4P530932EBI-29384,EBI-24124

Protein-protein interaction databases

BioGridi33046. 64 interactions.
DIPiDIP-6597N.
IntActiP51996. 6 interactions.
MINTiMINT-703976.
STRINGi4932.YGL210W.

Structurei

Secondary structure

1
222
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi11 – 199Combined sources
Helixi26 – 3510Combined sources
Beta strandi47 – 5711Combined sources
Beta strandi60 – 7112Combined sources
Helixi76 – 838Combined sources
Beta strandi88 – 947Combined sources
Helixi98 – 1025Combined sources
Helixi104 – 11411Combined sources
Beta strandi120 – 1267Combined sources
Helixi128 – 1336Combined sources
Helixi138 – 14710Combined sources
Beta strandi151 – 1544Combined sources
Turni157 – 1593Combined sources
Helixi163 – 17715Combined sources

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3RWMX-ray2.00B7-188[»]
3RWOX-ray1.70A/B7-188[»]
ProteinModelPortaliP51996.
SMRiP51996. Positions 7-178.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi42 – 509Effector regionCurated

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rab family.Curated

Phylogenomic databases

eggNOGiCOG1100.
GeneTreeiENSGT00760000118841.
HOGENOMiHOG000233968.
InParanoidiP51996.
KOiK07905.
OMAiIQVDAKT.
OrthoDBiEOG7BGHXR.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003579. Small_GTPase_Rab_type.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
PRINTSiPR00449. RASTRNSFRMNG.
SMARTiSM00175. RAB. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P51996-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSNEDYGYDY DYLFKIVLIG DSGVGKSNLL SRFTTDEFNI ESKSTIGVEF
60 70 80 90 100
ATRTIEVENK KIKAQIWDTA GQERYRAITS AYYRGAVGAL IVYDISKSSS
110 120 130 140 150
YENCNHWLTE LRENADDNVA VGLIGNKSDL AHLRAVPTDE AKNFAMENQM
160 170 180 190 200
LFTETSALNS DNVDKAFREL IVAIFQMVSK HQVDLSGSGT NNMGSNGAPK
210 220
GPTISLTPAP KEDKKKKSSN CC
Length:222
Mass (Da):24,520
Last modified:January 23, 2007 - v3
Checksum:i621217A296161964
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X72834 Genomic DNA. Translation: CAA51355.1.
U33754 Genomic DNA. Translation: AAC49495.1.
Z72732 Genomic DNA. Translation: CAA96926.1.
AY558506 Genomic DNA. Translation: AAS56832.1.
BK006941 Genomic DNA. Translation: DAA07906.1.
PIRiS58514.
RefSeqiNP_011305.1. NM_001181075.1.

Genome annotation databases

EnsemblFungiiYGL210W; YGL210W; YGL210W.
GeneIDi852662.
KEGGisce:YGL210W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X72834 Genomic DNA. Translation: CAA51355.1 .
U33754 Genomic DNA. Translation: AAC49495.1 .
Z72732 Genomic DNA. Translation: CAA96926.1 .
AY558506 Genomic DNA. Translation: AAS56832.1 .
BK006941 Genomic DNA. Translation: DAA07906.1 .
PIRi S58514.
RefSeqi NP_011305.1. NM_001181075.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3RWM X-ray 2.00 B 7-188 [» ]
3RWO X-ray 1.70 A/B 7-188 [» ]
ProteinModelPortali P51996.
SMRi P51996. Positions 7-178.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33046. 64 interactions.
DIPi DIP-6597N.
IntActi P51996. 6 interactions.
MINTi MINT-703976.
STRINGi 4932.YGL210W.

Proteomic databases

MaxQBi P51996.
PaxDbi P51996.
PeptideAtlasi P51996.
PRIDEi P51996.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YGL210W ; YGL210W ; YGL210W .
GeneIDi 852662.
KEGGi sce:YGL210W.

Organism-specific databases

SGDi S000003178. YPT32.

Phylogenomic databases

eggNOGi COG1100.
GeneTreei ENSGT00760000118841.
HOGENOMi HOG000233968.
InParanoidi P51996.
KOi K07905.
OMAi IQVDAKT.
OrthoDBi EOG7BGHXR.

Enzyme and pathway databases

BioCyci YEAST:G3O-30687-MONOMER.

Miscellaneous databases

NextBioi 971948.

Gene expression databases

Genevestigatori P51996.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
InterProi IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003579. Small_GTPase_Rab_type.
[Graphical view ]
Pfami PF00071. Ras. 1 hit.
[Graphical view ]
PRINTSi PR00449. RASTRNSFRMNG.
SMARTi SM00175. RAB. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
TIGRFAMsi TIGR00231. small_GTP. 1 hit.
PROSITEi PS51419. RAB. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Two GTPase isoforms, Ypt31p and Ypt32p, are essential for Golgi function in yeast."
    Benli M., Doering F., Robinson D.G., Yang X., Gallwitz D.
    EMBO J. 15:6460-6475(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
    Strain: DUR.
  2. "Lambda clone B22 contains a 7676 bp genomic fragment of Saccharomyces cerevisiae chromosome VII spanning the VAM7-SPM2 intergenic region and containing three novel transcribed open reading frames."
    Kail M., Juettner E., Vaux D.
    Yeast 12:799-807(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. "Analysis of 21.7 kb DNA sequence from the left arm of chromosome VII reveals 11 open reading frames: two correspond to new genes."
    Feuermann M., Simeonava L., Souciet J.-L., Potier S.
    Yeast 13:475-477(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  6. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  7. "Two new Ypt GTPases are required for exit from the yeast trans-Golgi compartment."
    Jedd G., Mulholland J., Segev N.
    J. Cell Biol. 137:563-580(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF ALA-141, SUBCELLULAR LOCATION.
  8. "Two new members of a family of Ypt/Rab GTPase activating proteins. Promiscuity of substrate recognition."
    Albert S., Gallwitz D.
    J. Biol. Chem. 274:33186-33189(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: ENZYME REGULATION.
  9. "The TRAPP complex is a nucleotide exchanger for Ypt1 and Ypt31/32."
    Jones S., Newman C., Liu F., Segev N.
    Mol. Biol. Cell 11:4403-4411(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: ENZYME REGULATION.
  10. "Saccharomyces cerevisiae Pra1p/Yip3p interacts with Yip1p and Rab proteins."
    Calero M., Collins R.N.
    Biochem. Biophys. Res. Commun. 290:676-681(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH YIP3.
  11. "Mutation of TRS130, which encodes a component of the TRAPP II complex, activates transcription of OCH1 in Saccharomyces cerevisiae."
    Yamamoto K., Jigami Y.
    Curr. Genet. 42:85-93(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH TRS130.
  12. "Identification of the novel proteins Yip4p and Yip5p as Rab GTPase interacting factors."
    Calero M., Winand N.J., Collins R.N.
    FEBS Lett. 515:89-98(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH YIF1; YIP4 AND YIP5.
  13. "Ypt32 recruits the Sec4p guanine nucleotide exchange factor, Sec2p, to secretory vesicles; evidence for a Rab cascade in yeast."
    Ortiz D., Medkova M., Walch-Solimena C., Novick P.J.
    J. Cell Biol. 157:1005-1015(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH SEC2, MUTAGENESIS OF SER-27; GLU-49; GLN-72 AND ASN-126.
  14. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  15. "Trs130 participates in autophagy through GTPases Ypt31/32 in Saccharomyces cerevisiae."
    Zou S., Chen Y., Liu Y., Segev N., Yu S., Liu Y., Min G., Ye M., Zeng Y., Zhu X., Hong B., Bjorn L.O., Liang Y., Li S., Xie Z.
    Traffic 14:233-246(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiYPT32_YEAST
AccessioniPrimary (citable) accession number: P51996
Secondary accession number(s): D6VTU5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: January 23, 2007
Last modified: October 29, 2014
This is version 137 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 4298 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

External Data

Dasty 3