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P51996 (YPT32_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 133. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
GTP-binding protein YPT32/YPT11
Alternative name(s):
Rab GTPase YPT32
Gene names
Name:YPT32
Synonyms:YPT11
Ordered Locus Names:YGL210W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length222 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for protein transport through the secretory pathway. Probably involved in regulation of secretory vesicle formation at the trans-Golgi compartment. Mediates the proper polarized localization of SEC2, a GEF for SEC4, but does not alter the exchange activity of SEC2 on SEC4. Plays a role in autophagy. Ref.1 Ref.7 Ref.11 Ref.13 Ref.15

Enzyme regulation

Alternates between an inactive form bound to GDP and an active form bound to GTP. Activated by the guanine nucleotide-exchange factor (GEF) TRAPP complex, and inactivated by GTPase-activating protein (GAP) GYP3. Ref.8 Ref.9

Subunit structure

Interacts with YIF1, YIP3, YIP4 and YIP5. Interacts with the GEF SEC2. Interacts with TRS130. Ref.10 Ref.11 Ref.12 Ref.13

Subcellular location

Golgi apparatus membrane; Lipid-anchor Ref.7.

Miscellaneous

Present with 4298 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the small GTPase superfamily. Rab family.

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 222222GTP-binding protein YPT32/YPT11
PRO_0000121324

Regions

Nucleotide binding20 – 278GTP By similarity
Nucleotide binding68 – 725GTP By similarity
Nucleotide binding126 – 1294GTP By similarity
Motif42 – 509Effector region Probable

Amino acid modifications

Lipidation2211S-geranylgeranyl cysteine By similarity
Lipidation2221S-geranylgeranyl cysteine By similarity

Experimental info

Mutagenesis271S → N: Binds preferentially GDP. Ref.13
Mutagenesis491E → Q: Cold sensitive. Ref.13
Mutagenesis721Q → L: Reduces GTPase activity. Ref.13
Mutagenesis1261N → I: Blocks nucleotide binding. Ref.13
Mutagenesis1411A → D: Loss of function at 37 degrees Celsius. Ref.7

Secondary structure

............................. 222
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P51996 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 621217A296161964

FASTA22224,520
        10         20         30         40         50         60 
MSNEDYGYDY DYLFKIVLIG DSGVGKSNLL SRFTTDEFNI ESKSTIGVEF ATRTIEVENK 

        70         80         90        100        110        120 
KIKAQIWDTA GQERYRAITS AYYRGAVGAL IVYDISKSSS YENCNHWLTE LRENADDNVA 

       130        140        150        160        170        180 
VGLIGNKSDL AHLRAVPTDE AKNFAMENQM LFTETSALNS DNVDKAFREL IVAIFQMVSK 

       190        200        210        220 
HQVDLSGSGT NNMGSNGAPK GPTISLTPAP KEDKKKKSSN CC 

« Hide

References

« Hide 'large scale' references
[1]"Two GTPase isoforms, Ypt31p and Ypt32p, are essential for Golgi function in yeast."
Benli M., Doering F., Robinson D.G., Yang X., Gallwitz D.
EMBO J. 15:6460-6475(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
Strain: DUR.
[2]"Lambda clone B22 contains a 7676 bp genomic fragment of Saccharomyces cerevisiae chromosome VII spanning the VAM7-SPM2 intergenic region and containing three novel transcribed open reading frames."
Kail M., Juettner E., Vaux D.
Yeast 12:799-807(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"Analysis of 21.7 kb DNA sequence from the left arm of chromosome VII reveals 11 open reading frames: two correspond to new genes."
Feuermann M., Simeonava L., Souciet J.-L., Potier S.
Yeast 13:475-477(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. expand/collapse author list , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[6]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[7]"Two new Ypt GTPases are required for exit from the yeast trans-Golgi compartment."
Jedd G., Mulholland J., Segev N.
J. Cell Biol. 137:563-580(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, MUTAGENESIS OF ALA-141, SUBCELLULAR LOCATION.
[8]"Two new members of a family of Ypt/Rab GTPase activating proteins. Promiscuity of substrate recognition."
Albert S., Gallwitz D.
J. Biol. Chem. 274:33186-33189(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: ENZYME REGULATION.
[9]"The TRAPP complex is a nucleotide exchanger for Ypt1 and Ypt31/32."
Jones S., Newman C., Liu F., Segev N.
Mol. Biol. Cell 11:4403-4411(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: ENZYME REGULATION.
[10]"Saccharomyces cerevisiae Pra1p/Yip3p interacts with Yip1p and Rab proteins."
Calero M., Collins R.N.
Biochem. Biophys. Res. Commun. 290:676-681(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH YIP3.
[11]"Mutation of TRS130, which encodes a component of the TRAPP II complex, activates transcription of OCH1 in Saccharomyces cerevisiae."
Yamamoto K., Jigami Y.
Curr. Genet. 42:85-93(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH TRS130.
[12]"Identification of the novel proteins Yip4p and Yip5p as Rab GTPase interacting factors."
Calero M., Winand N.J., Collins R.N.
FEBS Lett. 515:89-98(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH YIF1; YIP4 AND YIP5.
[13]"Ypt32 recruits the Sec4p guanine nucleotide exchange factor, Sec2p, to secretory vesicles; evidence for a Rab cascade in yeast."
Ortiz D., Medkova M., Walch-Solimena C., Novick P.J.
J. Cell Biol. 157:1005-1015(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH SEC2, MUTAGENESIS OF SER-27; GLU-49; GLN-72 AND ASN-126.
[14]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[15]"Trs130 participates in autophagy through GTPases Ypt31/32 in Saccharomyces cerevisiae."
Zou S., Chen Y., Liu Y., Segev N., Yu S., Liu Y., Min G., Ye M., Zeng Y., Zhu X., Hong B., Bjorn L.O., Liang Y., Li S., Xie Z.
Traffic 14:233-246(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X72834 Genomic DNA. Translation: CAA51355.1.
U33754 Genomic DNA. Translation: AAC49495.1.
Z72732 Genomic DNA. Translation: CAA96926.1.
AY558506 Genomic DNA. Translation: AAS56832.1.
BK006941 Genomic DNA. Translation: DAA07906.1.
PIRS58514.
RefSeqNP_011305.1. NM_001181075.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3RWMX-ray2.00B7-188[»]
3RWOX-ray1.70A/B7-188[»]
ProteinModelPortalP51996.
SMRP51996. Positions 7-178.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33046. 63 interactions.
DIPDIP-6597N.
IntActP51996. 6 interactions.
MINTMINT-703976.
STRING4932.YGL210W.

Proteomic databases

PaxDbP51996.
PeptideAtlasP51996.
PRIDEP51996.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYGL210W; YGL210W; YGL210W.
GeneID852662.
KEGGsce:YGL210W.

Organism-specific databases

SGDS000003178. YPT32.

Phylogenomic databases

eggNOGCOG1100.
GeneTreeENSGT00710000106372.
HOGENOMHOG000233968.
KOK07905.
OMAKQIRDSP.
OrthoDBEOG7BGHXR.

Enzyme and pathway databases

BioCycYEAST:G3O-30687-MONOMER.

Gene expression databases

GenevestigatorP51996.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
InterProIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003579. Small_GTPase_Rab_type.
[Graphical view]
PfamPF00071. Ras. 1 hit.
[Graphical view]
PRINTSPR00449. RASTRNSFRMNG.
SMARTSM00175. RAB. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
TIGRFAMsTIGR00231. small_GTP. 1 hit.
PROSITEPS51419. RAB. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio971948.

Entry information

Entry nameYPT32_YEAST
AccessionPrimary (citable) accession number: P51996
Secondary accession number(s): D6VTU5
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: January 23, 2007
Last modified: April 16, 2014
This is version 133 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references