Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

GTP-binding protein YPT32/YPT11

Gene

YPT32

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for protein transport through the secretory pathway. Probably involved in regulation of secretory vesicle formation at the trans-Golgi compartment. Mediates the proper polarized localization of SEC2, a GEF for SEC4, but does not alter the exchange activity of SEC2 on SEC4. Plays a role in autophagy.5 Publications

Enzyme regulationi

Alternates between an inactive form bound to GDP and an active form bound to GTP. Activated by the guanine nucleotide-exchange factor (GEF) TRAPP complex, and inactivated by GTPase-activating protein (GAP) GYP3.2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi20 – 27GTPBy similarity8
Nucleotide bindingi68 – 72GTPBy similarity5
Nucleotide bindingi126 – 129GTPBy similarity4

GO - Molecular functioni

  • GTPase activity Source: SGD
  • GTP binding Source: UniProtKB-KW

GO - Biological processi

  • autophagy Source: UniProtKB-KW
  • early endosome to Golgi transport Source: SGD
  • exocytosis Source: SGD
  • protein transport Source: UniProtKB-KW
  • small GTPase mediated signal transduction Source: InterPro
  • vesicle-mediated transport Source: SGD
Complete GO annotation...

Keywords - Biological processi

Autophagy, Exocytosis, Protein transport, Transport

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30687-MONOMER.
ReactomeiR-SCE-1445148. Translocation of GLUT4 to the plasma membrane.
R-SCE-6798695. Neutrophil degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
GTP-binding protein YPT32/YPT11
Alternative name(s):
Rab GTPase YPT32
Gene namesi
Name:YPT32
Synonyms:YPT11
Ordered Locus Names:YGL210W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGL210W.
SGDiS000003178. YPT32.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: GOC
  • endosome Source: SGD
  • Golgi apparatus Source: SGD
  • Golgi membrane Source: UniProtKB-SubCell
  • recycling endosome Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi27S → N: Binds preferentially GDP. 1 Publication1
Mutagenesisi49E → Q: Cold sensitive. 1 Publication1
Mutagenesisi72Q → L: Reduces GTPase activity. 1 Publication1
Mutagenesisi126N → I: Blocks nucleotide binding. 1 Publication1
Mutagenesisi141A → D: Loss of function at 37 degrees Celsius. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001213242 – 222GTP-binding protein YPT32/YPT11Add BLAST221

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Lipidationi221S-geranylgeranyl cysteineBy similarity1
Lipidationi222S-geranylgeranyl cysteineBy similarity1

Keywords - PTMi

Acetylation, Lipoprotein, Prenylation

Proteomic databases

MaxQBiP51996.
PRIDEiP51996.

PTM databases

iPTMnetiP51996.

Interactioni

Subunit structurei

Interacts with YIF1, YIP3, YIP4 and YIP5. Interacts with the GEF SEC2. Interacts with TRS130.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
YIF1P538452EBI-29384,EBI-28230
YIP3P536332EBI-29384,EBI-25301
YIP4P530932EBI-29384,EBI-24124

Protein-protein interaction databases

BioGridi33046. 69 interactors.
DIPiDIP-6597N.
IntActiP51996. 6 interactors.
MINTiMINT-703976.

Structurei

Secondary structure

1222
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi11 – 19Combined sources9
Helixi26 – 35Combined sources10
Beta strandi47 – 57Combined sources11
Beta strandi60 – 71Combined sources12
Helixi76 – 83Combined sources8
Beta strandi88 – 94Combined sources7
Helixi98 – 102Combined sources5
Helixi104 – 114Combined sources11
Beta strandi120 – 126Combined sources7
Helixi128 – 133Combined sources6
Helixi138 – 147Combined sources10
Beta strandi151 – 154Combined sources4
Turni157 – 159Combined sources3
Helixi163 – 177Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3RWMX-ray2.00B7-188[»]
3RWOX-ray1.70A/B7-188[»]
ProteinModelPortaliP51996.
SMRiP51996.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi42 – 50Effector regionCurated9

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rab family.Curated

Phylogenomic databases

GeneTreeiENSGT00760000118841.
HOGENOMiHOG000233968.
InParanoidiP51996.
KOiK07905.
OMAiANSTACK.
OrthoDBiEOG092C5MDH.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P51996-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNEDYGYDY DYLFKIVLIG DSGVGKSNLL SRFTTDEFNI ESKSTIGVEF
60 70 80 90 100
ATRTIEVENK KIKAQIWDTA GQERYRAITS AYYRGAVGAL IVYDISKSSS
110 120 130 140 150
YENCNHWLTE LRENADDNVA VGLIGNKSDL AHLRAVPTDE AKNFAMENQM
160 170 180 190 200
LFTETSALNS DNVDKAFREL IVAIFQMVSK HQVDLSGSGT NNMGSNGAPK
210 220
GPTISLTPAP KEDKKKKSSN CC
Length:222
Mass (Da):24,520
Last modified:January 23, 2007 - v3
Checksum:i621217A296161964
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X72834 Genomic DNA. Translation: CAA51355.1.
U33754 Genomic DNA. Translation: AAC49495.1.
Z72732 Genomic DNA. Translation: CAA96926.1.
AY558506 Genomic DNA. Translation: AAS56832.1.
BK006941 Genomic DNA. Translation: DAA07906.1.
PIRiS58514.
RefSeqiNP_011305.1. NM_001181075.1.

Genome annotation databases

EnsemblFungiiYGL210W; YGL210W; YGL210W.
GeneIDi852662.
KEGGisce:YGL210W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X72834 Genomic DNA. Translation: CAA51355.1.
U33754 Genomic DNA. Translation: AAC49495.1.
Z72732 Genomic DNA. Translation: CAA96926.1.
AY558506 Genomic DNA. Translation: AAS56832.1.
BK006941 Genomic DNA. Translation: DAA07906.1.
PIRiS58514.
RefSeqiNP_011305.1. NM_001181075.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3RWMX-ray2.00B7-188[»]
3RWOX-ray1.70A/B7-188[»]
ProteinModelPortaliP51996.
SMRiP51996.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33046. 69 interactors.
DIPiDIP-6597N.
IntActiP51996. 6 interactors.
MINTiMINT-703976.

PTM databases

iPTMnetiP51996.

Proteomic databases

MaxQBiP51996.
PRIDEiP51996.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGL210W; YGL210W; YGL210W.
GeneIDi852662.
KEGGisce:YGL210W.

Organism-specific databases

EuPathDBiFungiDB:YGL210W.
SGDiS000003178. YPT32.

Phylogenomic databases

GeneTreeiENSGT00760000118841.
HOGENOMiHOG000233968.
InParanoidiP51996.
KOiK07905.
OMAiANSTACK.
OrthoDBiEOG092C5MDH.

Enzyme and pathway databases

BioCyciYEAST:G3O-30687-MONOMER.
ReactomeiR-SCE-1445148. Translocation of GLUT4 to the plasma membrane.
R-SCE-6798695. Neutrophil degranulation.

Miscellaneous databases

PROiP51996.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiYPT32_YEAST
AccessioniPrimary (citable) accession number: P51996
Secondary accession number(s): D6VTU5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 155 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 4298 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.