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P51996

- YPT32_YEAST

UniProt

P51996 - YPT32_YEAST

Protein

GTP-binding protein YPT32/YPT11

Gene

YPT32

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 136 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Required for protein transport through the secretory pathway. Probably involved in regulation of secretory vesicle formation at the trans-Golgi compartment. Mediates the proper polarized localization of SEC2, a GEF for SEC4, but does not alter the exchange activity of SEC2 on SEC4. Plays a role in autophagy.5 Publications

    Enzyme regulationi

    Alternates between an inactive form bound to GDP and an active form bound to GTP. Activated by the guanine nucleotide-exchange factor (GEF) TRAPP complex, and inactivated by GTPase-activating protein (GAP) GYP3.2 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi20 – 278GTPBy similarity
    Nucleotide bindingi68 – 725GTPBy similarity
    Nucleotide bindingi126 – 1294GTPBy similarity

    GO - Molecular functioni

    1. GTPase activity Source: SGD
    2. GTP binding Source: UniProtKB-KW
    3. protein binding Source: IntAct

    GO - Biological processi

    1. autophagy Source: UniProtKB-KW
    2. early endosome to Golgi transport Source: SGD
    3. exocytosis Source: SGD
    4. GTP catabolic process Source: GOC
    5. protein transport Source: UniProtKB-KW
    6. small GTPase mediated signal transduction Source: InterPro
    7. vesicle-mediated transport Source: SGD

    Keywords - Biological processi

    Autophagy, Exocytosis, Protein transport, Transport

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30687-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    GTP-binding protein YPT32/YPT11
    Alternative name(s):
    Rab GTPase YPT32
    Gene namesi
    Name:YPT32
    Synonyms:YPT11
    Ordered Locus Names:YGL210W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VII

    Organism-specific databases

    SGDiS000003178. YPT32.

    Subcellular locationi

    Golgi apparatus membrane 1 Publication; Lipid-anchor 1 Publication

    GO - Cellular componenti

    1. endosome Source: SGD
    2. Golgi apparatus Source: SGD
    3. Golgi membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi27 – 271S → N: Binds preferentially GDP. 1 Publication
    Mutagenesisi49 – 491E → Q: Cold sensitive. 1 Publication
    Mutagenesisi72 – 721Q → L: Reduces GTPase activity. 1 Publication
    Mutagenesisi126 – 1261N → I: Blocks nucleotide binding. 1 Publication
    Mutagenesisi141 – 1411A → D: Loss of function at 37 degrees Celsius. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 222222GTP-binding protein YPT32/YPT11PRO_0000121324Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi221 – 2211S-geranylgeranyl cysteineBy similarity
    Lipidationi222 – 2221S-geranylgeranyl cysteineBy similarity

    Keywords - PTMi

    Lipoprotein, Prenylation

    Proteomic databases

    MaxQBiP51996.
    PaxDbiP51996.
    PeptideAtlasiP51996.
    PRIDEiP51996.

    Expressioni

    Gene expression databases

    GenevestigatoriP51996.

    Interactioni

    Subunit structurei

    Interacts with YIF1, YIP3, YIP4 and YIP5. Interacts with the GEF SEC2. Interacts with TRS130.4 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    YIF1P538452EBI-29384,EBI-28230
    YIP3P536332EBI-29384,EBI-25301
    YIP4P530932EBI-29384,EBI-24124

    Protein-protein interaction databases

    BioGridi33046. 63 interactions.
    DIPiDIP-6597N.
    IntActiP51996. 6 interactions.
    MINTiMINT-703976.
    STRINGi4932.YGL210W.

    Structurei

    Secondary structure

    1
    222
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi11 – 199
    Helixi26 – 3510
    Beta strandi47 – 5711
    Beta strandi60 – 7112
    Helixi76 – 838
    Beta strandi88 – 947
    Helixi98 – 1025
    Helixi104 – 11411
    Beta strandi120 – 1267
    Helixi128 – 1336
    Helixi138 – 14710
    Beta strandi151 – 1544
    Turni157 – 1593
    Helixi163 – 17715

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3RWMX-ray2.00B7-188[»]
    3RWOX-ray1.70A/B7-188[»]
    ProteinModelPortaliP51996.
    SMRiP51996. Positions 7-178.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi42 – 509Effector regionCurated

    Sequence similaritiesi

    Belongs to the small GTPase superfamily. Rab family.Curated

    Phylogenomic databases

    eggNOGiCOG1100.
    GeneTreeiENSGT00710000106372.
    HOGENOMiHOG000233968.
    KOiK07905.
    OMAiIQVDAKT.
    OrthoDBiEOG7BGHXR.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR001806. Small_GTPase.
    IPR003579. Small_GTPase_Rab_type.
    [Graphical view]
    PfamiPF00071. Ras. 1 hit.
    [Graphical view]
    PRINTSiPR00449. RASTRNSFRMNG.
    SMARTiSM00175. RAB. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR00231. small_GTP. 1 hit.
    PROSITEiPS51419. RAB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P51996-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSNEDYGYDY DYLFKIVLIG DSGVGKSNLL SRFTTDEFNI ESKSTIGVEF    50
    ATRTIEVENK KIKAQIWDTA GQERYRAITS AYYRGAVGAL IVYDISKSSS 100
    YENCNHWLTE LRENADDNVA VGLIGNKSDL AHLRAVPTDE AKNFAMENQM 150
    LFTETSALNS DNVDKAFREL IVAIFQMVSK HQVDLSGSGT NNMGSNGAPK 200
    GPTISLTPAP KEDKKKKSSN CC 222
    Length:222
    Mass (Da):24,520
    Last modified:January 23, 2007 - v3
    Checksum:i621217A296161964
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X72834 Genomic DNA. Translation: CAA51355.1.
    U33754 Genomic DNA. Translation: AAC49495.1.
    Z72732 Genomic DNA. Translation: CAA96926.1.
    AY558506 Genomic DNA. Translation: AAS56832.1.
    BK006941 Genomic DNA. Translation: DAA07906.1.
    PIRiS58514.
    RefSeqiNP_011305.1. NM_001181075.1.

    Genome annotation databases

    EnsemblFungiiYGL210W; YGL210W; YGL210W.
    GeneIDi852662.
    KEGGisce:YGL210W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X72834 Genomic DNA. Translation: CAA51355.1 .
    U33754 Genomic DNA. Translation: AAC49495.1 .
    Z72732 Genomic DNA. Translation: CAA96926.1 .
    AY558506 Genomic DNA. Translation: AAS56832.1 .
    BK006941 Genomic DNA. Translation: DAA07906.1 .
    PIRi S58514.
    RefSeqi NP_011305.1. NM_001181075.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3RWM X-ray 2.00 B 7-188 [» ]
    3RWO X-ray 1.70 A/B 7-188 [» ]
    ProteinModelPortali P51996.
    SMRi P51996. Positions 7-178.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33046. 63 interactions.
    DIPi DIP-6597N.
    IntActi P51996. 6 interactions.
    MINTi MINT-703976.
    STRINGi 4932.YGL210W.

    Proteomic databases

    MaxQBi P51996.
    PaxDbi P51996.
    PeptideAtlasi P51996.
    PRIDEi P51996.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YGL210W ; YGL210W ; YGL210W .
    GeneIDi 852662.
    KEGGi sce:YGL210W.

    Organism-specific databases

    SGDi S000003178. YPT32.

    Phylogenomic databases

    eggNOGi COG1100.
    GeneTreei ENSGT00710000106372.
    HOGENOMi HOG000233968.
    KOi K07905.
    OMAi IQVDAKT.
    OrthoDBi EOG7BGHXR.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30687-MONOMER.

    Miscellaneous databases

    NextBioi 971948.

    Gene expression databases

    Genevestigatori P51996.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR001806. Small_GTPase.
    IPR003579. Small_GTPase_Rab_type.
    [Graphical view ]
    Pfami PF00071. Ras. 1 hit.
    [Graphical view ]
    PRINTSi PR00449. RASTRNSFRMNG.
    SMARTi SM00175. RAB. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    TIGRFAMsi TIGR00231. small_GTP. 1 hit.
    PROSITEi PS51419. RAB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Two GTPase isoforms, Ypt31p and Ypt32p, are essential for Golgi function in yeast."
      Benli M., Doering F., Robinson D.G., Yang X., Gallwitz D.
      EMBO J. 15:6460-6475(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
      Strain: DUR.
    2. "Lambda clone B22 contains a 7676 bp genomic fragment of Saccharomyces cerevisiae chromosome VII spanning the VAM7-SPM2 intergenic region and containing three novel transcribed open reading frames."
      Kail M., Juettner E., Vaux D.
      Yeast 12:799-807(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. "Analysis of 21.7 kb DNA sequence from the left arm of chromosome VII reveals 11 open reading frames: two correspond to new genes."
      Feuermann M., Simeonava L., Souciet J.-L., Potier S.
      Yeast 13:475-477(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    4. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
      Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
      , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
      Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    6. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    7. "Two new Ypt GTPases are required for exit from the yeast trans-Golgi compartment."
      Jedd G., Mulholland J., Segev N.
      J. Cell Biol. 137:563-580(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, MUTAGENESIS OF ALA-141, SUBCELLULAR LOCATION.
    8. "Two new members of a family of Ypt/Rab GTPase activating proteins. Promiscuity of substrate recognition."
      Albert S., Gallwitz D.
      J. Biol. Chem. 274:33186-33189(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: ENZYME REGULATION.
    9. "The TRAPP complex is a nucleotide exchanger for Ypt1 and Ypt31/32."
      Jones S., Newman C., Liu F., Segev N.
      Mol. Biol. Cell 11:4403-4411(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: ENZYME REGULATION.
    10. "Saccharomyces cerevisiae Pra1p/Yip3p interacts with Yip1p and Rab proteins."
      Calero M., Collins R.N.
      Biochem. Biophys. Res. Commun. 290:676-681(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH YIP3.
    11. "Mutation of TRS130, which encodes a component of the TRAPP II complex, activates transcription of OCH1 in Saccharomyces cerevisiae."
      Yamamoto K., Jigami Y.
      Curr. Genet. 42:85-93(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH TRS130.
    12. "Identification of the novel proteins Yip4p and Yip5p as Rab GTPase interacting factors."
      Calero M., Winand N.J., Collins R.N.
      FEBS Lett. 515:89-98(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH YIF1; YIP4 AND YIP5.
    13. "Ypt32 recruits the Sec4p guanine nucleotide exchange factor, Sec2p, to secretory vesicles; evidence for a Rab cascade in yeast."
      Ortiz D., Medkova M., Walch-Solimena C., Novick P.J.
      J. Cell Biol. 157:1005-1015(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH SEC2, MUTAGENESIS OF SER-27; GLU-49; GLN-72 AND ASN-126.
    14. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    15. "Trs130 participates in autophagy through GTPases Ypt31/32 in Saccharomyces cerevisiae."
      Zou S., Chen Y., Liu Y., Segev N., Yu S., Liu Y., Min G., Ye M., Zeng Y., Zhu X., Hong B., Bjorn L.O., Liang Y., Li S., Xie Z.
      Traffic 14:233-246(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiYPT32_YEAST
    AccessioniPrimary (citable) accession number: P51996
    Secondary accession number(s): D6VTU5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 136 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 4298 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome VII
      Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

    External Data

    Dasty 3