Reviewed, UniProtKB/Swiss-Prot P51993 (FUT6_HUMAN)

Names and origin

Protein names

Recommended name:
    Alpha-(1,3)-fucosyltransferase
    EC=2.4.1.65
Alternative name(s):
    Galactoside 3-L-fucosyltransferase
    Fucosyltransferase 6
    FucT-VI

Gene names

Name:	FUT6
Synonyms:	FCT3A

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	359 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Enzyme involved in the biosynthesis of the E-Selectin ligand, sialyl-Lewis X. Catalyzes the transfer of fucose from GDP-beta-fucose to alpha-2,3 sialylated substrates.
Catalytic activity	GDP-beta-L-fucose + beta-D-galactosyl-(1->3)-N-acetyl-D-glucosaminyl-R = GDP + beta-D-galactosyl-(1->3)-(alpha-L-fucosyl-(1->4))-N-acetyl-beta-D-glucosaminyl-R.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus › Golgi stack membrane; Single-pass type II membrane protein. Note= Membrane-bound form in trans cisternae of Golgi.
Tissue specificity	Kidney, liver, colon, small intestine, bladder, uterus and salivary gland.
Sequence similarities	Belongs to the glycosyltransferase 10 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Signal-anchor Transmembrane
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	L-fucose catabolic process Non-traceable author statement. Source: UniProtKB protein amino acid glycosylation Traceable author statement. Source: UniProtKB
Cellular component	Golgi apparatus Traceable author statement. Source: UniProtKB integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3-galactosyl-N-acetylglucosaminide 4-alpha-L-fucosyltransferase activity Inferred from electronic annotation. Source: EC alpha(1,3)-fucosyltransferase activity Traceable author statement. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P51993-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P51993-2)

The sequence of this isoform differs from the canonical sequence as follows:
     348-359: RYQTRGIAAWFT → SSGGLIYLRTRLPEASPA

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 359	359	Alpha-(1,3)-fucosyltransferase		PRO_0000221110
Regions
	Topological domain	1 – 14	14	Cytoplasmic Potential
	Transmembrane	15 – 34	20	Signal-anchor for type II membrane protein Potential
	Topological domain	35 – 359	325	Lumenal Potential
Amino acid modifications
	Glycosylation	46	1	N-linked (GlcNAc...) Potential
	Glycosylation	91	1	N-linked (GlcNAc...) Potential
	Glycosylation	153	1	N-linked (GlcNAc...) Potential
	Glycosylation	184	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	348 – 359	12	RYQTR…AAWFT → SSGGLIYLRTRLPEASPA in isoform 2.		VSP_001780
	Natural variant	124	1	P → S: dbSNP rs778805.		VAR_024463
	Natural variant	230	1	Q → K: dbSNP rs364637.		VAR_024464

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 67ABDF058F0999DA	FASTA	359	41,860
	10 20 30 40 50 60 MDPLGPAKPQ WSWRCCLTTL LFQLLMAVCF FSYLRVSQDD PTVYPNGSRF PDSTGTPAHS 70 80 90 100 110 120 IPLILLWTWP FNKPIALPRC SEMVPGTADC NITADRKVYP QADAVIVHHR EVMYNPSAQL 130 140 150 160 170 180 PRSPRRQGQR WIWFSMESPS HCWQLKAMDG YFNLTMSYRS DSDIFTPYGW LEPWSGQPAH 190 200 210 220 230 240 PPLNLSAKTE LVAWAVSNWG PNSARVRYYQ SLQAHLKVDV YGRSHKPLPQ GTMMETLSRY 250 260 270 280 290 300 KFYLAFENSL HPDYITEKLW RNALEAWAVP VVLGPSRSNY ERFLPPDAFI HVDDFQSPKD 310 320 330 340 350 LARYLQELDK DHARYLSYFR WRETLRPRSF SWALAFCKAC WKLQEESRYQ TRGIAAWFT
	Isoform 2 [UniParc]. Checksum: CDC4FE3C793960BA		365	42,278
	10 20 30 40 50 60 MDPLGPAKPQ WSWRCCLTTL LFQLLMAVCF FSYLRVSQDD PTVYPNGSRF PDSTGTPAHS 70 80 90 100 110 120 IPLILLWTWP FNKPIALPRC SEMVPGTADC NITADRKVYP QADAVIVHHR EVMYNPSAQL 130 140 150 160 170 180 PRSPRRQGQR WIWFSMESPS HCWQLKAMDG YFNLTMSYRS DSDIFTPYGW LEPWSGQPAH 190 200 210 220 230 240 PPLNLSAKTE LVAWAVSNWG PNSARVRYYQ SLQAHLKVDV YGRSHKPLPQ GTMMETLSRY 250 260 270 280 290 300 KFYLAFENSL HPDYITEKLW RNALEAWAVP VVLGPSRSNY ERFLPPDAFI HVDDFQSPKD 310 320 330 340 350 360 LARYLQELDK DHARYLSYFR WRETLRPRSF SWALAFCKAC WKLQEESSSG GLIYLRTRLP EASPA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The cloning and expression of a human alpha-1,3 fucosyltransferase capable of forming the E-selectin ligand." Koszdin K.L., Bowen B.R. Biochem. Biophys. Res. Commun. 187:152-157(1992) [PubMed: 1520296] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Molecular cloning of a fourth member of a human alpha (1,3)fucosyltransferase gene family. Multiple homologous sequences that determine expression of the Lewis x, sialyl Lewis x, and difucosyl sialyl Lewis x epitopes." Weston B.W., Smith P.L., Kelly R.J., Lowe J.B. J. Biol. Chem. 267:24575-24584(1992) [PubMed: 1339443] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Expression of human chromosome 19p alpha(1,3)-fucosyltransferase genes in normal tissues. Alternative splicing, polyadenylation, and isoforms." Cameron H.S., Szczepaniak D., Weston B.W. J. Biol. Chem. 270:20112-20122(1995) [PubMed: 7650030] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Kidney.
[4]	"The DNA sequence and biology of human chromosome 19." Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M. Nature 428:529-535(2004) [PubMed: 15057824] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
+	Additional computationally mapped references.

Web resources

GGDB

GlycoGene database

Functional Glycomics Gateway - GTase

Fucosyltransferase 6

Cross-references

Sequence databases
EMBLGenBankDDBJ	M98825 mRNA. Translation: AAA99222.1. L01698 Genomic DNA. Translation: AAB03078.1. U27333 mRNA. Translation: AAC50192.1. U27334 mRNA. Translation: AAC50193.1. U27335 mRNA. Translation: AAC50194.1. U27336 mRNA. Translation: AAC50195.1. U27331 mRNA. Translation: AAC50190.1. U27332 mRNA. Translation: AAC50191.1. Sequence problems. U27337 mRNA. Translation: AAC50196.1. AL031258 Genomic DNA. No translation available. CH471139 Genomic DNA. Translation: EAW69136.1.
PIR	A45156. I39048. I39049.
RefSeq	NP_000141.1. NP_001035791.1.
UniGene	Hs.631846 Hs.705615
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSG00000156413. Homo sapiens. [Contig view]
GeneID	2528.
KEGG	hsa:2528.
Organism-specific databases
H-InvDB	HIX0014674.
HGNC	HGNC:4017. FUT6.
MIM	136836. gene+phenotype.
PharmGKB	PA28433.
GenAtlas	Search...
GeneCards	Search...
Phylogenomic databases
HOVERGEN	P51993.
Gene expression databases
ArrayExpress	P51993.
CleanEx	HS_FUT6.
GermOnline	ENSG00000156413. Homo sapiens.
Family and domain databases
InterPro	IPR001503. Glyco_trans_10. [Graphical view]
PANTHER	PTHR11929. Glyco_trans_10. 1 hit.
Pfam	PF00852. Glyco_transf_10. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	9961.
SOURCE	Search...

Entry information

Entry name

FUT6_HUMAN

Accession

Primary (citable) accession number: P51993
Secondary accession number(s): A6NEX0

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	November 25, 2008
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)