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Protein

ATP-dependent DNA helicase MER3

Gene

HFM1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

DNA-dependent ATPase. Required in the control of double strand breaks transition and crossover during meiosis. Unwinds DNA in the 3' TO 5' direction. Prefers single-stranded DNA.

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi161 – 168ATPPROSITE-ProRule annotation8
Zinc fingeri1039 – 1054C4-typeSequence analysisAdd BLAST16

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • DNA binding Source: UniProtKB-KW
  • DNA helicase activity Source: SGD
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • DNA unwinding involved in DNA replication Source: SGD
  • meiotic nuclear division Source: SGD
  • reciprocal meiotic recombination Source: SGD
  • synapsis Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Ligandi

ATP-binding, DNA-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-30721-MONOMER.
BRENDAi3.6.4.12. 984.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent DNA helicase MER3 (EC:3.6.4.12)
Alternative name(s):
Protein HFM1
Gene namesi
Name:HFM1
Synonyms:MER3
Ordered Locus Names:YGL251C
ORF Names:NRE1046
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGL251C.
SGDiS000003220. HFM1.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi166G → D: Decrease in activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001020901 – 1187ATP-dependent DNA helicase MER3Add BLAST1187

Proteomic databases

PRIDEiP51979.

Interactioni

Protein-protein interaction databases

BioGridi33028. 15 interactors.
DIPiDIP-2604N.
IntActiP51979. 3 interactors.
MINTiMINT-426642.

Structurei

3D structure databases

ProteinModelPortaliP51979.
SMRiP51979.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini148 – 322Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST175
Domaini360 – 542Helicase C-terminalPROSITE-ProRule annotationAdd BLAST183
Domaini616 – 922SEC63Add BLAST307

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi268 – 271DEIH box4

Sequence similaritiesi

Belongs to the helicase family. SKI2 subfamily.Curated
Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation
Contains 1 SEC63 domain.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri1039 – 1054C4-typeSequence analysisAdd BLAST16

Keywords - Domaini

Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00550000074822.
HOGENOMiHOG000093662.
InParanoidiP51979.
KOiK15271.
OMAiINRLIRC.
OrthoDBiEOG092C2VYL.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR004179. Sec63-dom.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
PF02889. Sec63. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SM00973. Sec63. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P51979-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTKFDRLGT GKRSRPSPNN IDFNDQSATF KRNKKNSRQP SFKVGLSYNS
60 70 80 90 100
LLDDCDDENE TEEIFEGRGL QFFDKDDNFS ITADDTQVTS KLFDHDLEQT
110 120 130 140 150
PDEEAKKPKK VTIRKSAKKC LSTTILPDSF RGVFKFTEFN KMQSEAFPSI
160 170 180 190 200
YESNENCIIS SPTGSGKTVL FELAILRLIK ETNSDTNNTK IIYIAPTKSL
210 220 230 240 250
CYEMYKNWFP SFVNLSVGML TSDTSFLETE KAKKCNIIIT TPEKWDLLTR
260 270 280 290 300
RWSDYSRLFE LVKLVLVDEI HTIKEKRGAS LEVILTRMNT MCQNIRFVAL
310 320 330 340 350
SATVPNIEDL ALWLKTNNEL PANILSFDES YRQVQLTKFV YGYSFNCKND
360 370 380 390 400
FQKDAIYNSK LIEIIEKHAD NRPVLIFCPT RASTISTAKF LLNNHIFSKS
410 420 430 440 450
KKRCNHNPSD KILNECMQQG IAFHHAGISL EDRTAVEKEF LAGSINILCS
460 470 480 490 500
TSTLAVGVNL PAYLVIIKGT KSWNSSEIQE YSDLDVLQMI GRAGRPQFET
510 520 530 540 550
HGCAVIMTDS KMKQTYENLI HGTDVLESSL HLNLIEHLAA ETSLETVYSI
560 570 580 590 600
ETAVNWLRNT FFYVRFGKNP AAYQEVNRYV SFHSVEDSQI NQFCQYLLDT
610 620 630 640 650
LVKVKIIDIS NGEYKSTAYG NAMTRHYISF ESMKQFINAK KFLSLQGILN
660 670 680 690 700
LLATSEEFSV MRVRHNEKKL FKEINLSPLL KYPFLTEKKQ SQIIDRVSQK
710 720 730 740 750
VSLLIQYELG GLEFPSYEGA SKLHQTLVQD KFLVFRHCFR LLKCMVDTFI
760 770 780 790 800
EKSDGTSLKN TLFLLRSLNG HCWENTPMVL RQLKTIGLVS VRRLIRHGIT
810 820 830 840 850
NLEEMGHLSD TQIEYYLNLK IGNGIKIKND ISLLPCLNIR TKLENCKIEN
860 870 880 890 900
EELWLTFKVE ISATFKSSIW HGQHLSLDIE TEKSSGELID FRRLQVNKLQ
910 920 930 940 950
SPRGFRISAK ISPKLEKIEF SIHCQEIAGL GKTIVYSTDH LASQFSAKTP
960 970 980 990 1000
NIRKDLNSLE KCLFYESSSD GEVGKTSRVS HKDGLEESLS SDDSILDYLN
1010 1020 1030 1040 1050
ERKKSSKAVE SAAVIHPEAH SSSHFSNGRQ VRSNGNYECF HSCKDKTQCR
1060 1070 1080 1090 1100
HLCCKEGIPV KYIKEKGPSS IKPVSKPDQI RQPLLAKNIN TTPHLEKRLN
1110 1120 1130 1140 1150
SKPKQWQEEN TDIATVHTLP SKIYNLSQQM SSMEAGEQVL KSGPENCPEI
1160 1170 1180
IPIDLESSDS YSSNTAASSI SDPNGDLDFL GSDIEFE
Length:1,187
Mass (Da):135,072
Last modified:September 5, 2006 - v3
Checksum:i6B804F1E3D12A5B9
GO

Sequence cautioni

The sequence AAA93159 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAA64136 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAA96971 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti552T → TAA in AAA93159 (Ref. 4) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X94357 Genomic DNA. Translation: CAA64136.1. Sequence problems.
Z72773 Genomic DNA. Translation: CAA96971.1. Sequence problems.
U22156 Genomic DNA. Translation: AAA93159.1. Different initiation.
BK006941 Genomic DNA. Translation: DAA07868.1.
RefSeqiNP_011263.2. NM_001181117.1.

Genome annotation databases

EnsemblFungiiYGL251C; YGL251C; YGL251C.
GeneIDi852641.
KEGGisce:YGL251C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X94357 Genomic DNA. Translation: CAA64136.1. Sequence problems.
Z72773 Genomic DNA. Translation: CAA96971.1. Sequence problems.
U22156 Genomic DNA. Translation: AAA93159.1. Different initiation.
BK006941 Genomic DNA. Translation: DAA07868.1.
RefSeqiNP_011263.2. NM_001181117.1.

3D structure databases

ProteinModelPortaliP51979.
SMRiP51979.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33028. 15 interactors.
DIPiDIP-2604N.
IntActiP51979. 3 interactors.
MINTiMINT-426642.

Proteomic databases

PRIDEiP51979.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGL251C; YGL251C; YGL251C.
GeneIDi852641.
KEGGisce:YGL251C.

Organism-specific databases

EuPathDBiFungiDB:YGL251C.
SGDiS000003220. HFM1.

Phylogenomic databases

GeneTreeiENSGT00550000074822.
HOGENOMiHOG000093662.
InParanoidiP51979.
KOiK15271.
OMAiINRLIRC.
OrthoDBiEOG092C2VYL.

Enzyme and pathway databases

BioCyciYEAST:G3O-30721-MONOMER.
BRENDAi3.6.4.12. 984.

Miscellaneous databases

PROiP51979.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR004179. Sec63-dom.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
PF02889. Sec63. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SM00973. Sec63. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHFM1_YEAST
AccessioniPrimary (citable) accession number: P51979
Secondary accession number(s): D6VV84
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: September 5, 2006
Last modified: November 2, 2016
This is version 146 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.