P51979 (HFM1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ATP-dependent DNA helicase MER3
EC=3.6.4.12

Alternative name(s):
Protein HFM1

Gene names

Name:	HFM1
Synonyms:	MER3
Ordered Locus Names:	YGL251C
ORF Names:	NRE1046

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	1187 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	DNA-dependent ATPase. Required in the control of double strand breaks transition and crossover during meiosis. Unwinds DNA in the 3' TO 5' direction. Prefers single-stranded DNA.
Catalytic activity	ATP + H2O = ADP + phosphate.
Subcellular location	Nucleus Probable.
Sequence similarities	Belongs to the helicase family. SKI2 subfamily. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain. Contains 1 SEC63 domain.
Sequence caution	The sequence AAA93159.1 differs from that shown. Reason: Erroneous initiation. The sequence CAA64136.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAA96971.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Nucleus
Domain	Zinc-finger
Ligand	ATP-binding DNA-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Helicase Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA unwinding involved in replication Inferred from direct assay. Source: SGD reciprocal meiotic recombination Inferred from mutant phenotype. Source: SGD synapsis Inferred from genetic interaction. Source: SGD
Cellular component	nucleus Inferred from direct assay. Source: SGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent helicase activity Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: UniProtKB-KW DNA helicase activity Inferred from direct assay. Source: SGD metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1187	1187	ATP-dependent DNA helicase MER3		PRO_0000102090
Regions
	Domain	148 – 322	175	Helicase ATP-binding
	Domain	360 – 542	183	Helicase C-terminal
	Domain	616 – 922	307	SEC63
	Nucleotide binding	161 – 168	8	ATP By similarity
	Zinc finger	1039 – 1054	16	C4-type Potential
	Motif	268 – 271	4	DEIH box
Experimental info
	Mutagenesis	166	1	G → D: Decrease in activity. Ref.6
	Sequence conflict	552	1	T → TAA in AAA93159. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P51979 [UniParc]. Last modified September 5, 2006. Version 3. Checksum: 6B804F1E3D12A5B9	FASTA	1,187	135,072
	10 20 30 40 50 60 MKTKFDRLGT GKRSRPSPNN IDFNDQSATF KRNKKNSRQP SFKVGLSYNS LLDDCDDENE 70 80 90 100 110 120 TEEIFEGRGL QFFDKDDNFS ITADDTQVTS KLFDHDLEQT PDEEAKKPKK VTIRKSAKKC 130 140 150 160 170 180 LSTTILPDSF RGVFKFTEFN KMQSEAFPSI YESNENCIIS SPTGSGKTVL FELAILRLIK 190 200 210 220 230 240 ETNSDTNNTK IIYIAPTKSL CYEMYKNWFP SFVNLSVGML TSDTSFLETE KAKKCNIIIT 250 260 270 280 290 300 TPEKWDLLTR RWSDYSRLFE LVKLVLVDEI HTIKEKRGAS LEVILTRMNT MCQNIRFVAL 310 320 330 340 350 360 SATVPNIEDL ALWLKTNNEL PANILSFDES YRQVQLTKFV YGYSFNCKND FQKDAIYNSK 370 380 390 400 410 420 LIEIIEKHAD NRPVLIFCPT RASTISTAKF LLNNHIFSKS KKRCNHNPSD KILNECMQQG 430 440 450 460 470 480 IAFHHAGISL EDRTAVEKEF LAGSINILCS TSTLAVGVNL PAYLVIIKGT KSWNSSEIQE 490 500 510 520 530 540 YSDLDVLQMI GRAGRPQFET HGCAVIMTDS KMKQTYENLI HGTDVLESSL HLNLIEHLAA 550 560 570 580 590 600 ETSLETVYSI ETAVNWLRNT FFYVRFGKNP AAYQEVNRYV SFHSVEDSQI NQFCQYLLDT 610 620 630 640 650 660 LVKVKIIDIS NGEYKSTAYG NAMTRHYISF ESMKQFINAK KFLSLQGILN LLATSEEFSV 670 680 690 700 710 720 MRVRHNEKKL FKEINLSPLL KYPFLTEKKQ SQIIDRVSQK VSLLIQYELG GLEFPSYEGA 730 740 750 760 770 780 SKLHQTLVQD KFLVFRHCFR LLKCMVDTFI EKSDGTSLKN TLFLLRSLNG HCWENTPMVL 790 800 810 820 830 840 RQLKTIGLVS VRRLIRHGIT NLEEMGHLSD TQIEYYLNLK IGNGIKIKND ISLLPCLNIR 850 860 870 880 890 900 TKLENCKIEN EELWLTFKVE ISATFKSSIW HGQHLSLDIE TEKSSGELID FRRLQVNKLQ 910 920 930 940 950 960 SPRGFRISAK ISPKLEKIEF SIHCQEIAGL GKTIVYSTDH LASQFSAKTP NIRKDLNSLE 970 980 990 1000 1010 1020 KCLFYESSSD GEVGKTSRVS HKDGLEESLS SDDSILDYLN ERKKSSKAVE SAAVIHPEAH 1030 1040 1050 1060 1070 1080 SSSHFSNGRQ VRSNGNYECF HSCKDKTQCR HLCCKEGIPV KYIKEKGPSS IKPVSKPDQI 1090 1100 1110 1120 1130 1140 RQPLLAKNIN TTPHLEKRLN SKPKQWQEEN TDIATVHTLP SKIYNLSQQM SSMEAGEQVL 1150 1160 1170 1180 KSGPENCPEI IPIDLESSDS YSSNTAASSI SDPNGDLDFL GSDIEFE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence of a 39,411 bp DNA fragment covering the left end of chromosome VII of Saccharomyces cerevisiae." Coissac E., Maillier E., Robineau S., Netter P. Yeast 12:1555-1562(1996) [PubMed: 8972578] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Saccharomyces cerevisiae Hfm1p (HFM1) gene." West R.W. Jr., Thomas S., Ma J.L., Finley R.L. Jr. Submitted (MAR-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 21-1187.
[5]	"The Saccharomyces cerevisiae MER3 gene, encoding a novel helicase-like protein, is required for crossover control in meiosis." Nakagawa T., Ogawa H. EMBO J. 18:5714-5723(1999) [PubMed: 10523314] [Abstract] Cited for: CHARACTERIZATION.
[6]	"The mer3 helicase involved in meiotic crossing over is stimulated by single-stranded DNA-binding proteins and unwinds DNA in the 3' to 5' direction." Nakagawa T., Flores-Rozas H., Kolodner R.D. J. Biol. Chem. 276:31487-31493(2001) [PubMed: 11376001] [Abstract] Cited for: CHARACTERIZATION, MUTAGENESIS OF GLY-166.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X94357 Genomic DNA. Translation: CAA64136.1. Sequence problems. Z72773 Genomic DNA. Translation: CAA96971.1. Sequence problems. U22156 Genomic DNA. Translation: AAA93159.1. Different initiation. BK006941 Genomic DNA. Translation: DAA07868.1.
RefSeq	NP_011263.2. NM_001181117.1.
3D structure databases
ProteinModelPortal	P51979.
SMR	P51979. Positions 126-832.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2604N.
IntAct	P51979. 2 interactions.
MINT	MINT-426642.
STRING	P51979.
Proteomic databases
PeptideAtlas	P51979.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YGL251C; YGL251C; YGL251C.
GeneID	852641.
KEGG	sce:YGL251C.
NMPDR	fig|4932.3.peg.2366.
Organism-specific databases
CYGD	YGL251c.
SGD	S000003220. HFM1.
Phylogenomic databases
eggNOG	fuNOG04376.
GeneTree	EFGT00050000000530.
HOGENOM	HBG396227.
OMA	GNYECNH.
OrthoDB	EOG4WWVSR.
Gene expression databases
ArrayExpress	P51979.
Genevestigator	P51979.
GermOnline	YGL251C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR003593. ATPase_AAA+_core. IPR014001. DEAD-like_helicase. IPR011545. DNA/RNA_helicase_DEAD/DEAH_N. IPR001650. Helicase_C. IPR004179. Sec63-dom. [Graphical view]
KO	K15271.
Pfam	PF00270. DEAD. 1 hit. PF00271. Helicase_C. 1 hit. PF02889. Sec63. 1 hit. [Graphical view]
SMART	SM00382. AAA. 1 hit. SM00487. DEXDc. 1 hit. SM00490. HELICc. 1 hit. SM00611. SEC63. 1 hit. SM00973. Sec63. 1 hit. [Graphical view]
PROSITE	PS51192. HELICASE_ATP_BIND_1. 1 hit. PS51194. HELICASE_CTER. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	971894.

Entry information

Entry name

HFM1_YEAST

Accession

Primary (citable) accession number: P51979
Secondary accession number(s): D6VV84

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	September 5, 2006
Last modified:	December 14, 2011
This is version 107 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program