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Protein

ATP-dependent DNA helicase MER3

Gene

HFM1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

DNA-dependent ATPase. Required in the control of double strand breaks transition and crossover during meiosis. Unwinds DNA in the 3' TO 5' direction. Prefers single-stranded DNA.

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi161 – 168ATPPROSITE-ProRule annotation8
Zinc fingeri1039 – 1054C4-typeSequence analysisAdd BLAST16

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ATP-dependent RNA helicase activity Source: GO_Central
  • DNA helicase activity Source: SGD
  • heteroduplex DNA loop binding Source: SGD
  • metal ion binding Source: UniProtKB-KW
  • MutLbeta complex binding Source: SGD

GO - Biological processi

  • DNA unwinding involved in DNA replication Source: SGD
  • meiotic cell cycle Source: SGD
  • protein localization to site of double-strand break Source: SGD
  • reciprocal meiotic recombination Source: SGD
  • RNA secondary structure unwinding Source: GO_Central
  • synapsis Source: SGD

Keywordsi

Molecular functionDNA-binding, Helicase, Hydrolase
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-30721-MONOMER
BRENDAi3.6.4.12 984

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent DNA helicase MER3 (EC:3.6.4.12)
Alternative name(s):
Protein HFM1
Gene namesi
Name:HFM1
Synonyms:MER3
Ordered Locus Names:YGL251C
ORF Names:NRE1046
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGL251C
SGDiS000003220 HFM1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi166G → D: Decrease in activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001020901 – 1187ATP-dependent DNA helicase MER3Add BLAST1187

Proteomic databases

PaxDbiP51979
PRIDEiP51979

Interactioni

Protein-protein interaction databases

BioGridi33028, 30 interactors
DIPiDIP-2604N
IntActiP51979, 3 interactors
MINTiP51979
STRINGi4932.YGL251C

Structurei

3D structure databases

ProteinModelPortaliP51979
SMRiP51979
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini148 – 322Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST175
Domaini360 – 542Helicase C-terminalPROSITE-ProRule annotationAdd BLAST183
Domaini616 – 922SEC63Add BLAST307

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi268 – 271DEIH box4

Sequence similaritiesi

Belongs to the helicase family. SKI2 subfamily.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri1039 – 1054C4-typeSequence analysisAdd BLAST16

Keywords - Domaini

Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00550000074822
HOGENOMiHOG000093662
InParanoidiP51979
KOiK15271
OMAiWDSITRK
OrthoDBiEOG092C2VYL

Family and domain databases

CDDicd00079 HELICc, 1 hit
InterProiView protein in InterPro
IPR011545 DEAD/DEAH_box_helicase_dom
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR027417 P-loop_NTPase
IPR004179 Sec63-dom
PfamiView protein in Pfam
PF00270 DEAD, 1 hit
PF00271 Helicase_C, 1 hit
PF02889 Sec63, 1 hit
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00490 HELICc, 1 hit
SM00973 Sec63, 1 hit
SUPFAMiSSF52540 SSF52540, 2 hits
PROSITEiView protein in PROSITE
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit

Sequencei

Sequence statusi: Complete.

P51979-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTKFDRLGT GKRSRPSPNN IDFNDQSATF KRNKKNSRQP SFKVGLSYNS
60 70 80 90 100
LLDDCDDENE TEEIFEGRGL QFFDKDDNFS ITADDTQVTS KLFDHDLEQT
110 120 130 140 150
PDEEAKKPKK VTIRKSAKKC LSTTILPDSF RGVFKFTEFN KMQSEAFPSI
160 170 180 190 200
YESNENCIIS SPTGSGKTVL FELAILRLIK ETNSDTNNTK IIYIAPTKSL
210 220 230 240 250
CYEMYKNWFP SFVNLSVGML TSDTSFLETE KAKKCNIIIT TPEKWDLLTR
260 270 280 290 300
RWSDYSRLFE LVKLVLVDEI HTIKEKRGAS LEVILTRMNT MCQNIRFVAL
310 320 330 340 350
SATVPNIEDL ALWLKTNNEL PANILSFDES YRQVQLTKFV YGYSFNCKND
360 370 380 390 400
FQKDAIYNSK LIEIIEKHAD NRPVLIFCPT RASTISTAKF LLNNHIFSKS
410 420 430 440 450
KKRCNHNPSD KILNECMQQG IAFHHAGISL EDRTAVEKEF LAGSINILCS
460 470 480 490 500
TSTLAVGVNL PAYLVIIKGT KSWNSSEIQE YSDLDVLQMI GRAGRPQFET
510 520 530 540 550
HGCAVIMTDS KMKQTYENLI HGTDVLESSL HLNLIEHLAA ETSLETVYSI
560 570 580 590 600
ETAVNWLRNT FFYVRFGKNP AAYQEVNRYV SFHSVEDSQI NQFCQYLLDT
610 620 630 640 650
LVKVKIIDIS NGEYKSTAYG NAMTRHYISF ESMKQFINAK KFLSLQGILN
660 670 680 690 700
LLATSEEFSV MRVRHNEKKL FKEINLSPLL KYPFLTEKKQ SQIIDRVSQK
710 720 730 740 750
VSLLIQYELG GLEFPSYEGA SKLHQTLVQD KFLVFRHCFR LLKCMVDTFI
760 770 780 790 800
EKSDGTSLKN TLFLLRSLNG HCWENTPMVL RQLKTIGLVS VRRLIRHGIT
810 820 830 840 850
NLEEMGHLSD TQIEYYLNLK IGNGIKIKND ISLLPCLNIR TKLENCKIEN
860 870 880 890 900
EELWLTFKVE ISATFKSSIW HGQHLSLDIE TEKSSGELID FRRLQVNKLQ
910 920 930 940 950
SPRGFRISAK ISPKLEKIEF SIHCQEIAGL GKTIVYSTDH LASQFSAKTP
960 970 980 990 1000
NIRKDLNSLE KCLFYESSSD GEVGKTSRVS HKDGLEESLS SDDSILDYLN
1010 1020 1030 1040 1050
ERKKSSKAVE SAAVIHPEAH SSSHFSNGRQ VRSNGNYECF HSCKDKTQCR
1060 1070 1080 1090 1100
HLCCKEGIPV KYIKEKGPSS IKPVSKPDQI RQPLLAKNIN TTPHLEKRLN
1110 1120 1130 1140 1150
SKPKQWQEEN TDIATVHTLP SKIYNLSQQM SSMEAGEQVL KSGPENCPEI
1160 1170 1180
IPIDLESSDS YSSNTAASSI SDPNGDLDFL GSDIEFE
Length:1,187
Mass (Da):135,072
Last modified:September 5, 2006 - v3
Checksum:i6B804F1E3D12A5B9
GO

Sequence cautioni

The sequence AAA93159 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAA64136 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAA96971 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti552T → TAA in AAA93159 (Ref. 4) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X94357 Genomic DNA Translation: CAA64136.1 Sequence problems.
Z72773 Genomic DNA Translation: CAA96971.1 Sequence problems.
U22156 Genomic DNA Translation: AAA93159.1 Different initiation.
BK006941 Genomic DNA Translation: DAA07868.1
RefSeqiNP_011263.2, NM_001181117.1

Genome annotation databases

EnsemblFungiiYGL251C; YGL251C; YGL251C
GeneIDi852641
KEGGisce:YGL251C

Similar proteinsi

Entry informationi

Entry nameiHFM1_YEAST
AccessioniPrimary (citable) accession number: P51979
Secondary accession number(s): D6VV84
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: September 5, 2006
Last modified: May 23, 2018
This is version 161 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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