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Reviewed, UniProtKB/Swiss-Prot P51977 (AL1A1_SHEEP)

Last modified June 16, 2009. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Retinal dehydrogenase 1
      Short name=RALDH 1
      Short name=RalDH1
    EC=1.2.1.36
Alternative name(s):
    Aldehyde dehydrogenase family 1 member A1
    Aldehyde dehydrogenase, cytosolic
    ALHDII
    ALDH-E1
Gene names
Name: ALDH1A1
Synonyms: ALDH1
OrganismOvis aries (Sheep)
Taxonomic identifier9940 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Caprinae › Ovis

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Protein attributes

Sequence length501 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Binds free retinal and cellular retinol-binding protein-bound retinal. Can convert/oxidize retinaldehyde to retinoic acid.

Catalytic activity

Retinal + NAD+ + H2O = retinoate + NADH.

Pathway

Cofactor metabolism; retinol metabolism.

Subunit structure

Homotetramer.

Subcellular location

Cytoplasm.

Sequence similarities

Belongs to the aldehyde dehydrogenase family.

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Ontologies

Keywords
   Cellular componentCytoplasm
   LigandNAD
   Molecular functionOxidoreductase
   PTMAcetylation
   Technical term3D-structure
Gene Ontology (GO)
   Biological processoxidation reduction

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionretinal dehydrogenase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 501500Retinal dehydrogenase 1
PRO_0000056420

Regions

Nucleotide binding246 – 2516NAD By similarity

Sites

Active site2691Proton acceptor
Active site3031Nucleophile
Site1701Transition state stabilizer

Amino acid modifications

Modified residue21N-acetylserine By similarity

Secondary structure

1 ................................................................................... 501
Helix Strand Turn

Details...

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Sequences

Sequence LengthMass (Da)Tools
P51977-1 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: 58B897197D621AB1

FASTA50154,825
        10         20         30         40         50         60 
MSSSAMPDVP APLTNLQFKY TKIFINNEWH SSVSGKKFPV FNPATEEKLC EVEEGDKEDV 

        70         80         90        100        110        120 
DKAVKAARQA FQIGSPWRTM DASERGRLLN KLADLIERDR LLLATMEAMN GGKLFSNAYL 

       130        140        150        160        170        180 
MDLGGCIKTL RYCAGWADKI QGRTIPMDGN FFTYTRSEPV GVCGQIIPWN FPLLMFLWKI 

       190        200        210        220        230        240 
GPALSCGNTV VVKPAEQTPL TALHMGSLIK EAGFPPGVVN IVPGYGPTAG AAISSHMDVD 

       250        260        270        280        290        300 
KVAFTGSTEV GKLIKEAAGK SNLKRVSLEL GGKSPCIVFA DADLDNAVEF AHQGVFYHQG 

       310        320        330        340        350        360 
QCCIAASRLF VEESIYDEFV RRSVERAKKY VLGNPLTPGV SQGPQIDKEQ YEKILDLIES 

       370        380        390        400        410        420 
GKKEGAKLEC GGGPWGNKGY FIQPTVFSDV TDDMRIAKEE IFGPVQQIMK FKSLDDVIKR 

       430        440        450        460        470        480 
ANNTFYGLSA GIFTNDIDKA ITVSSALQSG TVWVNCYSVV SAQCPFGGFK MSGNGRELGE 

       490        500 
YGFHEYTEVK TVTIKISQKN S

« Hide

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References

[1]"Cloning and characterisation of the cDNA for sheep liver cytosolic aldehyde dehydrogenase."
Stayner C.K., Tweedie J.W.
Adv. Exp. Med. Biol. 372:61-66(1995) [PubMed: 7484410] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Liver.
[2]"Sheep liver cytosolic aldehyde dehydrogenase: the structure reveals the basis for the retinal specificity of class 1 aldehyde dehydrogenases."
Moore S.A., Baker H.M., Blythe T.J., Kitson K.E., Kitson T.M., Baker E.N.
Structure 6:1541-1551(1998) [PubMed: 9862807] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.35 ANGSTROMS).
Tissue: Liver.

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Cross-references

Sequence databases

U12761 mRNA. Translation: AAA85435.1.
PIRS14752. S78582.
RefSeqNP_001009778.1.
UniGeneOar.873

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
1BXSX-ray2.35A/B/C/D1-501[»]
ModBaseSearch...

Genome annotation databases

GeneID443343.

Phylogenomic databases

HOVERGENP51977.

Enzyme and pathway databases

BRENDA1.2.1.36. 271.

Family and domain databases

InterProIPR016160. Ald_DH_CS.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH.
[Graphical view]
Gene3DG3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHERPTHR11699. Aldehyde_dehyd. 1 hit.
PfamPF00171. Aldedh. 1 hit.
[Graphical view]
PROSITEPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameAL1A1_SHEEP
AccessionPrimary (citable) accession number: P51977
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: January 23, 2007
Last modified: June 16, 2009
This is version 69 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents