P51976 (DHI2_RABIT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Corticosteroid 11-beta-dehydrogenase isozyme 2
EC=1.1.1.-

Alternative name(s):
11-beta-hydroxysteroid dehydrogenase type 2
Short name=11-DH2
Short name=11-beta-HSD2
NAD-dependent 11-beta-hydroxysteroid dehydrogenase

Gene names

Name:

HSD11B2

Organism

Oryctolagus cuniculus (Rabbit) [Reference proteome]

Taxonomic identifier

9986 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Lagomorpha › Leporidae › Oryctolagus

Protein attributes

Sequence length	406 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the conversion of cortisol to the inactive metabolite cortisone. Modulates intracellular glucocorticoid levels, thus protecting the nonselective mineralocorticoid receptor from occupation by glucocorticoids.
Catalytic activity	An 11-beta-hydroxysteroid + NAD+ = an 11-oxosteroid + NADH.
Subunit structure	Interacts with ligand-free cytoplasmic NR3C2 By similarity.
Subcellular location	Microsome. Endoplasmic reticulum Potential.
Tissue specificity	Highly expressed in the kidney.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Microsome
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	nucleotide binding Inferred from electronic annotation. Source: InterPro oxidoreductase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 406	406	Corticosteroid 11-beta-dehydrogenase isozyme 2		PRO_0000054629
Regions
	Nucleotide binding	82 – 111	30	NAD By similarity
Sites
	Active site	232	1	Proton acceptor By similarity
	Binding site	219	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P51976 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: F14579663918425D	FASTA	406	44,182
	10 20 30 40 50 60 MERWPWPSGG AWLLVAARAL IQLLRADLRL GRPLLAALAL LAALDWLCQS LLPPSAALAV 70 80 90 100 110 120 LAAAGWIALS RLARPQRLPV ATRAVLITGC DSGFGKETAK KLDAMGFTVL ATVLEMNGPG 130 140 150 160 170 180 ALELRACCSP RLKLLQMDLT KPADISRALE FTKAHTTSTG LWGLVNNAGH NDVVADVELS 190 200 210 220 230 240 PVATFRNCME VNFFGALELT KGLLPLLHHS RGRIVTLGSP AGEMPYPCLA AYGTSKAAMA 250 260 270 280 290 300 LLMDAFSCEL LPWGVKVSVI QPGCFKTESV SNVSHWEQRK QLLLANLPGE LRQAYGEDYI 310 320 330 340 350 360 EHLHREFLHS LRLALPDLSP VVDAITDALL AARPRPRYYP GRGLGLMYFI HYYLPEGLRR 370 380 390 400 RFLQSFFIIP CLPRALRPGQ PGATPAPDTA QDNPNPNPDP SLVGAR

References

[1]

"Expression cloning of the aldosterone target cell-specific 11 beta-hydroxysteroid dehydrogenase from rabbit collecting duct cells." Naray-Fejes-Toth A., Fejes-Toth G. Endocrinology 136:2579-2586(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: New Zealand white. Tissue: Kidney cortex.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U23835 mRNA. Translation: AAA86387.1.
PIR	I46535.
RefSeq	NP_001076221.1. NM_001082752.1.
UniGene	Ocu.2046.
3D structure databases
ProteinModelPortal	P51976.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	100009531.
Organism-specific databases
CTD	3291.
Phylogenomic databases
eggNOG	COG1028.
HOVERGEN	HBG005482.
OrthoDB	EOG49CQ89.
Family and domain databases
Gene3D	3.40.50.720. 1 hit.
InterPro	IPR002198. DH_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. IPR020904. Sc_DH/Rdtase_CS. [Graphical view]
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH.
PROSITE	PS00061. ADH_SHORT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHI2_RABIT

Accession

Primary (citable) accession number: P51976

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	April 3, 2013
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program