Reviewed, UniProtKB/Swiss-Prot P51975 (DHI1_SHEEP)

Names and origin

Protein names

Recommended name:
    Corticosteroid 11-beta-dehydrogenase isozyme 1
    EC=1.1.1.146
Alternative name(s):
    11-beta-hydroxysteroid dehydrogenase 1
      Short name=11-beta-HSD1
      Short name=11-DH

Gene names

Name:

HSD11B1

Organism

Ovis aries (Sheep)

Taxonomic identifier

9940 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Caprinae › Ovis

Protein attributes

Sequence length	292 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes reversibly the conversion of corticosterone to 11-dehydrocorticosterone.
Catalytic activity	An 11-beta-hydroxysteroid + NADP+ = an 11-oxosteroid + NADPH.
Subunit structure	Homodimer By similarity.
Subcellular location	Endoplasmic reticulum membrane; Single-pass type II membrane protein.
Tissue specificity	Liver, kidney, lung, hypothalamus, anterior pituitary and placenta.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Ontologies

Keywords
Biological process	Lipid metabolism Steroid metabolism
Cellular component	Endoplasmic reticulum Membrane
Domain	Signal-anchor Transmembrane
Ligand	NADP
Molecular function	Oxidoreductase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW steroid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	11-beta-hydroxysteroid dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: EC binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 292	291	Corticosteroid 11-beta-dehydrogenase isozyme 1		PRO_0000054625
Regions
	Topological domain	2 – 7	6	Cytoplasmic Potential
	Transmembrane	8 – 24	17	Signal-anchor for type II membrane protein Potential
	Topological domain	25 – 292	268	Lumenal Potential
	Nucleotide binding	34 – 63	30	NADP By similarity
Sites
	Active site	183	1	Proton acceptor By similarity
	Binding site	170	1	Substrate By similarity
Amino acid modifications
	Glycosylation	95	1	N-linked (GlcNAc...) Potential
	Glycosylation	207	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P51975-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: ADBD024E73EB95E3	FASTA	292	32,154
	10 20 30 40 50 60 MAFMKKYLLP ILGIFLAYYY YSANEEFRPE MLRGKRVIVT GASKGIGREM AYHLARMGAH 70 80 90 100 110 120 VVVTARSEES LKKVVSRCLE LGAASAHYVA GTMENMTFAE QFVAKAGELV GGLDMLILNH 130 140 150 160 170 180 INYTPLRVFS NDIHLLRRSL EVNLLSYVVL STAALPMLKQ TSGSIVVVSS VAGKIACPLA 190 200 210 220 230 240 AAYSASKFAL DGFFSSLRTE YEATKVNVSI TLCILGLIDT DTAMKAVAGI YNAEASPKEE 250 260 270 280 290 CALEIIKGGA LRQDEVYYDN SILTSLLLKN PGRKIMEFLS LKKYNMERFI NN

References

[1]

"Cloning of an ovine 11 beta-hydroxysteroid dehydrogenase complementary deoxyribonucleic acid: tissue and temporal distribution of its messenger ribonucleic acid during fetal and neonatal development." Yang K., Smith C.L., Dales D., Hammond G.L., Challis J.R. Endocrinology 131:2120-2126(1992) [PubMed: 1425412] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X69561 mRNA. Translation: CAA49290.1.
PIR	S33117.
RefSeq	NP_001009395.1.
UniGene	Oar.418
3D structure databases
SMR	P51975. Positions 23-288, 24-289.
ModBase	Search...
Genome annotation databases
GeneID	443409.
Phylogenomic databases
HOVERGEN	P51975.
Enzyme and pathway databases
BRENDA	1.1.1.146. 271.
Family and domain databases
InterPro	IPR002198. DH_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR19410. ADH_short_C2. 1 hit.
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH.
PROSITE	PS00061. ADH_SHORT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHI1_SHEEP

Accession

Primary (citable) accession number: P51975

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 61 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)