P51974 (PAX8_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Paired box protein Pax-8

Gene names

Name:	Pax8
Synonyms:	Pax-8

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	457 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Thought to encode a transcription factor. It may have a role in kidney cell differentiation. May play a regulatory role in mammalian development By similarity.
Subunit structure	Interacts with WWTR1 By similarity.
Subcellular location	Nucleus.
Sequence similarities	Contains 1 paired domain.

Ontologies

Keywords
Biological process	Differentiation Transcription Transcription regulation
Cellular component	Nucleus
Domain	Paired box
Ligand	DNA-binding
Molecular function	Developmental protein
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	metanephric collecting duct development Inferred from sequence or structural similarity. Source: UniProtKB metanephric distal convoluted tubule development Inferred from sequence or structural similarity. Source: UniProtKB metanephric nephron tubule formation Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of mesenchymal stem cell apoptosis involved in metanephric nephron morphogenesis Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of branching involved in ureteric bud morphogenesis Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of mesenchymal to epithelial transition involved in metanephros morphogenesis Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of metanephric DCT cell differentiation Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of thyroid hormone generation Inferred from sequence or structural similarity. Source: UniProtKB pronephric field specification Inferred from sequence or structural similarity. Source: UniProtKB regulation of metanephric nephron tubule epithelial cell differentiation Inferred from sequence or structural similarity. Source: UniProtKB regulation of thyroid-stimulating hormone secretion Inferred from sequence or structural similarity. Source: UniProtKB sulfur compound metabolic process Inferred from expression pattern. Source: RGD thyroid gland development Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	nucleoplasm Inferred from direct assay. Source: UniProtKB
Molecular function	RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Inferred from direct assay. Source: RGD RNA polymerase II core promoter sequence-specific DNA binding Inferred from sequence or structural similarity. Source: UniProtKB protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Nkx2-1	P23441	5	EBI-1223113,EBI-1223127
Wbp2	Q8R478	4	EBI-1223113,EBI-1223229

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 457	457	Paired box protein Pax-8		PRO_0000050199
Regions
	Domain	9 – 135	127	Paired
	Compositional bias	316 – 328	13	Ser-rich
Amino acid modifications
	Modified residue	280	1	Phosphothreonine Ref.3
	Modified residue	284	1	Phosphothreonine Ref.3
Experimental info
	Sequence conflict	183	1	N → H in CAA63930. Ref.1
	Sequence conflict	191	1	P → A in CAA63930. Ref.1
	Sequence conflict	195 – 199	5	NKRKM → THRGRC in CAA63930. Ref.1
	Sequence conflict	205 – 206	2	DS → GQ in CAA63930. Ref.1
	Sequence conflict	377	1	T → S in CAA63930. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P51974 [UniParc]. Last modified September 5, 2006. Version 2. Checksum: 50946E0829936D62	FASTA	457	48,806
	10 20 30 40 50 60 MPHNSIRSGH GGLNQLGGAF VNGRPLPEVV RQRIVDLAHQ GVRPCDISRQ LRVSHGCVSK 70 80 90 100 110 120 ILGRYYETGS IRPGVIGGSK PKVATPKVVE KIGDYKRQNP TMFAWEIRDR LLAEGVCDND 130 140 150 160 170 180 TVPSVSSINR IIRTKVQQPF NLPMDSCVAT KSLSPGHTLI PSSAVTPPES PQSDSLGSTY 190 200 210 220 230 240 SINGLLGIAQ PGSDNKRKMD DSDQDSCRLS IDSQSSSSGP RKHLRTDTFG QHHLEPLECP 250 260 270 280 290 300 FERQHYPEAY ASPSHTKGEQ GLYPLPLLNS TLDDGKATLT PSNTPLGRNL STHQTYPVVA 310 320 330 340 350 360 DPHSPYAIKQ ETPELSSSSS TPSSLSSSAF LDLQQVGSGG PAGASVPPFN AFSHAASVYG 370 380 390 400 410 420 QFTGQALLSG REMVGPTLPG YPPHIPTSGQ GSYASSAIAG MVAGSEYSGN AYSHTPYASY 430 440 450 SEAWRFPNSS LLSSPYYYSS TTRPSAPPTS ATAFDHL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Zannini M. Submitted (DEC-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Thyroid.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.
[3]	"Quantitative phosphoproteomics of vasopressin-sensitive renal cells: regulation of aquaporin-2 phosphorylation at two sites." Hoffert J.D., Pisitkun T., Wang G., Shen R.-F., Knepper M.A. Proc. Natl. Acad. Sci. U.S.A. 103:7159-7164(2006) [PubMed: 16641100] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-280 AND THR-284, MASS SPECTROMETRY. Tissue: Renal collecting duct.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X94246 mRNA. Translation: CAA63930.1. BC081779 mRNA. Translation: AAH81779.1.
IPI	IPI00207402.
RefSeq	NP_112403.2. NM_031141.2.
UniGene	Rn.10392.
3D structure databases
ProteinModelPortal	P51974.
SMR	P51974. Positions 12-135.
ModBase	Search...
Protein-protein interaction databases
IntAct	P51974. 2 interactions.
STRING	P51974.
PTM databases
PhosphoSite	P51974.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000019088; ENSRNOP00000019088; ENSRNOG00000026203.
GeneID	81819.
KEGG	rno:81819.
UCSC	NM_031141. rat.
Organism-specific databases
CTD	7849.
RGD	620434. Pax8.
Phylogenomic databases
eggNOG	roNOG13717.
GeneTree	ENSGT00600000084204.
HOVERGEN	HBG009115.
InParanoid	P51974.
OrthoDB	EOG42NJ0G.
Gene expression databases
ArrayExpress	P51974.
Genevestigator	P51974.
GermOnline	ENSRNOG00000026203. Rattus norvegicus.
Family and domain databases
InterPro	IPR009057. Homeodomain-like. IPR001523. Paired_box_N. IPR022130. Pax2_C. IPR011991. WHTH_trsnscrt_rep_DNA-bd. [Graphical view]
Gene3D	G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 2 hits.
KO	K09293.
Pfam	PF00292. PAX. 1 hit. PF12403. Pax2_C. 1 hit. [Graphical view]
PRINTS	PR00027. PAIREDBOX.
SMART	SM00351. PAX. 1 hit. [Graphical view]
SUPFAM	SSF46689. Homeodomain_like. 1 hit.
PROSITE	PS00034. PAIRED_1. 1 hit. PS51057. PAIRED_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	615735.

Entry information

Entry name

PAX8_RAT

Accession

Primary (citable) accession number: P51974
Secondary accession number(s): Q66HM8

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	September 5, 2006
Last modified:	November 16, 2011
This is version 90 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program