Reviewed, UniProtKB/Swiss-Prot P51965 (UB2E1_HUMAN)

Names and origin

Protein names

Recommended name:
    Ubiquitin-conjugating enzyme E2 E1
    EC=6.3.2.19
Alternative name(s):
    Ubiquitin-protein ligase E1
    Ubiquitin carrier protein E1
    UbcH6

Gene names

Name:	UBE2E1
Synonyms:	UBCH6

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	193 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the covalent attachment of ubiquitin to other proteins. Mediates the selective degradation of short-lived and abnormal proteins.
Catalytic activity	ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Interacts with RNF14.
Sequence similarities	Belongs to the ubiquitin-conjugating enzyme family.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Molecular function	Ligase
Technical term	3D-structure
Gene Ontology (GO)
Biological process	ISG15-protein conjugation Inferred from direct assay. Source: HGNC anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process Inferred from Experiment. Source: Reactome negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle Inferred from Experiment. Source: Reactome positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle Inferred from Experiment. Source: Reactome protein ubiquitination Inferred from direct assay. Source: UniProtKB regulation of protein metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytosol Inferred from Experiment. Source: Reactome nucleoplasm Inferred from Experiment. Source: Reactome
Molecular function	ISG15 ligase activity Inferred from direct assay. Source: HGNC protein binding Inferred from physical interaction. Source: IntAct ubiquitin-protein ligase activity Ref.1 Traceable author statement. Source: ProtInc
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
RNF5	Q99942	1	EBI-348546,EBI-348482

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 193

193

Ubiquitin-conjugating enzyme E2 E1

PRO_0000082470

Regions

Compositional bias

9 – 18

10

Poly-Ser

Sites

Active site

131

1

Glycyl thioester intermediate By similarity

Secondary structure

1

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193

Helix Strand Turn

Details...

Helix

22 – 24

3

Helix

42 – 61

20

Beta strand

67 – 74

8

Beta strand

78 – 82

5

Beta strand

95 – 101

7

Turn

104 – 108

5

Beta strand

112 – 117

6

Helix

133 – 135

3

Turn

136 – 138

3

Helix

145 – 157

13

Helix

167 – 175

9

Helix

177 – 191

15

Sequences

	Sequence		Length	Mass (Da)
	P51965-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 2FBC50BE2A6A0008	FASTA	193	21,404
	10 20 30 40 50 60 MSDDDSRAST SSSSSSSSNQ QTEKETNTPK KKESKVSMSK NSKLLSTSAK RIQKELADIT 70 80 90 100 110 120 LDPPPNCSAG PKGDNIYEWR STILGPPGSV YEGGVFFLDI TFTPEYPFKP PKVTFRTRIY 130 140 150 160 170 180 HCNINSQGVI CLDILKDNWS PALTISKVLL SICSLLTDCN PADPLVGSIA TQYMTNRAEH 190 DRMARQWTKR YAT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of human ubiquitin-conjugating enzymes UbcH6 and UbcH7 (E2-F1) and characterization of their interaction with E6-AP and RSP5." Nuber U., Schwarz S., Kaiser P., Schneider R., Scheffner M. J. Biol. Chem. 271:2795-2800(1996) [PubMed: 8576257] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Colon.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

X92963 mRNA. Translation: CAA63539.1.
BC009139 mRNA. Translation: AAH09139.1.

RefSeq

NP_003332.1.
NP_872607.1.

UniGene

Hs.164853

3D structure databases

PDBMSD

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1XR9	X-ray	1.79	C	83-91	[»]
3BZH	X-ray	1.60	A	1-193	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

P51965.

PTM databases

PhosphoSite

P51965.

Genome annotation databases

Ensembl

ENSG00000170142. Homo sapiens. [Contig view]

GeneID

7324.

KEGG

hsa:7324.

Organism-specific databases

H-InvDB

HIX0003129.

HGNC

HGNC:12477. UBE2E1.

MIM

602916. gene.

PharmGKB

PA37127.

GenAtlas

Search...

GeneCards

Search...

Phylogenomic databases

HOGENOM

P51965.

HOVERGEN

P51965.

Enzyme and pathway databases

Reactome

REACT_152. Cell Cycle, Mitotic.
REACT_1538. Cell Cycle Checkpoints.
REACT_6850. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
REACT_8017. APC-Cdc20 mediated degradation of Nek2A.
REACT_9035. APC/C:Cdh1-mediated degradation of Skp2.

Gene expression databases

CleanEx

HS_UBE2E1.

GermOnline

ENSG00000170142. Homo sapiens.

Family and domain databases

InterPro

IPR016135. UBQ-conjugat/RWD-like.
IPR000608. UBQ-conjugat_E2.
[Graphical view]

Gene3D

G3DSA:3.10.110.10. UBQ-conjugat_E2. 1 hit.

PANTHER

PTHR11621. UBQ-conjugat_E2. 1 hit.

Pfam

PF00179. UQ_con. 1 hit.
[Graphical view]

ProDom

PD000461. UBQ_conjugat. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SMART

SM00212. UBCc. 1 hit.
[Graphical view]

PROSITE

PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

LinkHub

P51965.

NextBio

28656.

SOURCE

Search...

Entry information

Entry name

UB2E1_HUMAN

Accession

Primary (citable) accession number: P51965

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	November 25, 2008
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)