Reviewed, UniProtKB/Swiss-Prot P51961 (RISA_PHOPO)

Names and origin

Protein names

Recommended name:
    Riboflavin synthase alpha chain
    EC=2.5.1.9

Gene names

Name:

ribE

Organism

Photobacterium phosphoreum

Taxonomic identifier

659 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Photobacterium

Protein attributes

Sequence length	218 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)-ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2-butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The alpha subunit catalyzes the dismutation of 6,7-dimethyl-8-lumazine to riboflavin and 5-amino-6-(1'-D)-ribityl-amino-2,4(1H,3H)-pyrimidinedione.
Catalytic activity	2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine.
Pathway	Cofactor biosynthesis; riboflavin biosynthesis; 5-amino-6-(D-ribitylamino)uracil and riboflavin from 6,7-dimethyl-8-(1-D-ribityl)lumazine: step 1/1.
Subunit structure	Oligomer that consist of 3 alpha subunits and 60 beta subunits By similarity.
Sequence similarities	Contains 2 lumazine-binding repeats.

Ontologies

Keywords
Biological process	Riboflavin biosynthesis
Domain	Repeat
Molecular function	Transferase
Gene Ontology (GO)
Biological process	riboflavin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	riboflavin synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 218	218	Riboflavin synthase alpha chain		PRO_0000068171
Regions
	Repeat	1 – 97	97	Lumazine-binding 1
	Repeat	98 – 194	97	Lumazine-binding 2

Sequences

	Sequence		Length	Mass (Da)
	P51961-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 309B197A71430975	FASTA	218	23,639
	10 20 30 40 50 60 MFTGIIEAVG NISAITSKGS DFEVSVNCDT LDLADVKIGD SIATNGICLT VVKLTANSYV 70 80 90 100 110 120 ADLSIETLSR TAFNYYKVGQ AVNLEKAMLP TTRFGGHIVS GHVDAVAEVI ECRTSGRAID 130 140 150 160 170 180 IWIRVPSQIE KYLSEKGSVT VDGVSLTVNA VTGNEFKLTI VPHTVVETTI ADFKVGNKVN 190 200 210 IEVDVLARYI ERLLLVDKPE DKQSKISMDL LERNGFLL

References

[1]	"Riboflavin synthesis genes are linked with the lux operon of Photobacterium phosphoreum." Lee C.Y., O'Kane D.J., Meighen E.A. J. Bacteriol. 176:2100-2104(1994) [PubMed: 8144477] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: DSM 2167 / LMG 4231 / NCIMB 844.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L11391 Genomic DNA. Translation: AAA25628.1.
3D structure databases
HSSP	HSSP built from PDB template 1I8D based on UniProtKB P29015.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.5.1.9. 348.
Family and domain databases
InterPro	IPR001783. Lumazine_bd. [Graphical view]
PANTHER	PTHR21098. Lumazine_bd. 1 hit.
Pfam	PF00677. Lum_binding. 2 hits. [Graphical view]
PIRSF	PIRSF000498. Riboflavin_syn_A. 1 hit.
ProDom	PD004110. Lum_binding. 2 hits. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00187. ribE. 1 hit.
PROSITE	PS51177. LUMAZINE_BIND. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RISA_PHOPO

Accession

Primary (citable) accession number: P51961

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	September 22, 2009
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)