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Protein

Myb-related protein A

Gene

Mybl1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Strong transcriptional activator; DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'. Could have a role in the proliferation and/or differentiation of neurogenic, spermatogenic and B-lymphoid cells.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi58 – 8124H-T-H motifPROSITE-ProRule annotationAdd
BLAST
DNA bindingi110 – 13324H-T-H motifPROSITE-ProRule annotationAdd
BLAST
DNA bindingi161 – 18424H-T-H motifPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Myb-related protein A
Short name:
A-Myb
Alternative name(s):
Myb-like protein 1
Gene namesi
Name:Mybl1
Synonyms:Amyb
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:99925. Mybl1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 751751Myb-related protein APRO_0000197055Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei393 – 3931N6-acetyllysineBy similarity
Cross-linki601 – 601Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Ubl conjugation

Proteomic databases

MaxQBiP51960.
PaxDbiP51960.
PRIDEiP51960.

PTM databases

PhosphoSiteiP51960.

Expressioni

Tissue specificityi

Predominantly in the testis. Very low levels in the ovaries, spleen and brain.

Developmental stagei

During embryogenesis it is predominantly expressed in several regions of the developing central nervous system and the urogenital ridge. Expression in the CNS is confined to the neural tube, the hindbrain, the neural retina and the olfactory epithelium, and coincides with the presence of proliferating immature neuronal precursor cells. In the adult mouse, A-Myb is expressed at high levels in type A spermatogonia (stem cells), and preleptotene and pachytene spermatocytes, with concomitant down-regulation of expression upon terminal differentiation of these cells into mature spermatozoa, and in B lymphocytes located in germinal centers of the spleen.

Gene expression databases

BgeeiP51960.
CleanExiMM_MYBL1.

Interactioni

Subunit structurei

Component of the DREAM complex (also named LINC complex) at least composed of E2F4, E2F5, LIN9, LIN37, LIN52, LIN54, MYBL1, MYBL2, RBL1, RBL2, RBBP4, TFDP1 and TFDP2. The complex exists in quiescent cells where it represses cell cycle-dependent genes. It dissociates in S phase when LIN9, LIN37, LIN52 and LIN54 form a subcomplex that binds to MYBL2 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000086034.

Structurei

3D structure databases

ProteinModelPortaliP51960.
SMRiP51960. Positions 35-186.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini30 – 8152HTH myb-type 1PROSITE-ProRule annotationAdd
BLAST
Domaini82 – 13756HTH myb-type 2PROSITE-ProRule annotationAdd
BLAST
Domaini138 – 18851HTH myb-type 3PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni230 – 29465Transcriptional activation domainBy similarityAdd
BLAST
Regioni297 – 552256Negative regulatory domainBy similarityAdd
BLAST

Sequence similaritiesi

Contains 3 HTH myb-type DNA-binding domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0048. Eukaryota.
COG5147. LUCA.
HOGENOMiHOG000231021.
HOVERGENiHBG007964.
InParanoidiP51960.
KOiK09421.
OrthoDBiEOG7H791P.
PhylomeDBiP51960.
TreeFamiTF326257.

Family and domain databases

Gene3Di1.10.10.60. 3 hits.
InterProiIPR015395. C-myb_C.
IPR009057. Homeodomain-like.
IPR017930. Myb_dom.
IPR001005. SANT/Myb.
IPR012642. Tscrpt_reg_Wos2-domain.
[Graphical view]
PfamiPF09316. Cmyb_C. 1 hit.
PF07988. LMSTEN. 1 hit.
PF00249. Myb_DNA-binding. 3 hits.
[Graphical view]
SMARTiSM00717. SANT. 3 hits.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 2 hits.
PROSITEiPS51294. HTH_MYB. 3 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Long (identifier: P51960-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAKRSRSEDE DDDLQYADHD YEVPQQKGLK KLWNRVKWTR DEDDKLKKLV
60 70 80 90 100
EQHGTDDWTL IASHLQNRSD FQCQHRWQKV LNPELIKGPW TKEEDQRVIE
110 120 130 140 150
LVQKYGPKRW SLIAKHLKGR IGKQCRERWH NHLNPEVKKS SWTEEEDRII
160 170 180 190 200
YEAHKRLGNR WAEIAKLLPG RTDNSIKNHW NSTMRRKVEQ EGYLQDGIKS
210 220 230 240 250
ERSSSKLQHK PCATMDHLQT QNQFYIPVQI PGYQYVSPDG NCVEHVQTSA
260 270 280 290 300
FIQQPFVDED PDKEKKIKEL ELLLMSAENE VRRKRLPPQP GSFSSWSGSF
310 320 330 340 350
LMDDSMSNTL NNLEEHTTEF YSMDENQTVS AQQNSPTKFL AVEANAVLSS
360 370 380 390 400
LQTIPEFPRT LELIESDPVA WSDVTSFDLS DAAASPVKST PVKLMRIQHN
410 420 430 440 450
EGAMECQFNV SLVLEGKKNS RNGGDSEAIP LTSPNVVKFS TPPTISRKKK
460 470 480 490 500
RIRVGQSAGS ELGSASLSEV GNRRIKHTPV KTLPFSPSQF FNTCPGNEQL
510 520 530 540 550
NIENPSFTST PICGQKVLIT TPLQKEATPK DQKENVGFRT PTIRRSILGT
560 570 580 590 600
TPRTPTPFKN ALAAQEKKYG PLKIVSQPLA FLEEDIREVL KEETGTDIFL
610 620 630 640 650
KEEDEPAYKS CKQEHSASVK KVRKSLALES WDKEEPGTQL LTEDISDMQS
660 670 680 690 700
ENILTTSLLM IPLLEIHDNR CNLTPEKQDI NSANKTYTLN KKRPNPNPCK
710 720 730 740 750
AVKLEKSLQS NCEWETVVYG KTEDQLIMTE QARRYLSTYT ATSSTSRALI

L
Length:751
Mass (Da):85,728
Last modified:November 1, 1997 - v2
Checksum:i20A4124BE9B82235
GO
Isoform Short (identifier: P51960-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     649-708: Missing.

Show »
Length:691
Mass (Da):78,913
Checksum:i943A75EA1702B88B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti358 – 3592PR → AE in AAA62182 (PubMed:8084617).Curated
Sequence conflicti446 – 4461S → L in AAA62182 (PubMed:8084617).Curated
Sequence conflicti464 – 4652SA → DG in AAA62182 (PubMed:8084617).Curated
Sequence conflicti473 – 4753RRI → AAL in AAA62182 (PubMed:8084617).Curated
Sequence conflicti751 – 7511L → LYCY in CAA57771 (PubMed:7813437).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti48 – 481K → R.
Natural varianti404 – 4041M → V.
Natural varianti447 – 4471R → G.
Natural varianti708 – 7081L → P.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei649 – 70860Missing in isoform Short. 1 PublicationVSP_003300Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X82327 mRNA. Translation: CAA57771.1.
L35261 mRNA. Translation: AAA62182.1.
CCDSiCCDS35510.1. [P51960-1]
CCDS69861.1. [P51960-2]
PIRiI49497.
RefSeqiNP_032677.2. NM_008651.3.
UniGeneiMm.459569.
Mm.479.

Genome annotation databases

GeneIDi17864.
KEGGimmu:17864.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X82327 mRNA. Translation: CAA57771.1.
L35261 mRNA. Translation: AAA62182.1.
CCDSiCCDS35510.1. [P51960-1]
CCDS69861.1. [P51960-2]
PIRiI49497.
RefSeqiNP_032677.2. NM_008651.3.
UniGeneiMm.459569.
Mm.479.

3D structure databases

ProteinModelPortaliP51960.
SMRiP51960. Positions 35-186.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000086034.

PTM databases

PhosphoSiteiP51960.

Proteomic databases

MaxQBiP51960.
PaxDbiP51960.
PRIDEiP51960.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi17864.
KEGGimmu:17864.

Organism-specific databases

CTDi4603.
MGIiMGI:99925. Mybl1.

Phylogenomic databases

eggNOGiKOG0048. Eukaryota.
COG5147. LUCA.
HOGENOMiHOG000231021.
HOVERGENiHBG007964.
InParanoidiP51960.
KOiK09421.
OrthoDBiEOG7H791P.
PhylomeDBiP51960.
TreeFamiTF326257.

Miscellaneous databases

ChiTaRSiMybl1. mouse.
NextBioi292629.
PROiP51960.
SOURCEiSearch...

Gene expression databases

BgeeiP51960.
CleanExiMM_MYBL1.

Family and domain databases

Gene3Di1.10.10.60. 3 hits.
InterProiIPR015395. C-myb_C.
IPR009057. Homeodomain-like.
IPR017930. Myb_dom.
IPR001005. SANT/Myb.
IPR012642. Tscrpt_reg_Wos2-domain.
[Graphical view]
PfamiPF09316. Cmyb_C. 1 hit.
PF07988. LMSTEN. 1 hit.
PF00249. Myb_DNA-binding. 3 hits.
[Graphical view]
SMARTiSM00717. SANT. 3 hits.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 2 hits.
PROSITEiPS51294. HTH_MYB. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Mouse A-myb encodes a trans-activator and is expressed in mitotically active cells of the developing central nervous system, adult testis and B lymphocytes."
    Trauth K., Mutschler B., Jenkins N.A., Gilbert D.J., Copeland N.G., Klempnauer K.H.
    EMBO J. 13:5994-6005(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LONG).
    Strain: BALB/cJ.
  2. "Murine A-myb: evidence for differential splicing and tissue-specific expression."
    Mettus R.V., Litvin J., Wali A., Toscani A., Latham K., Hatton K., Reddy E.P.
    Oncogene 9:3077-3086(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT).
    Strain: BALB/cJ.
    Tissue: Testis.
  3. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Testis.

Entry informationi

Entry nameiMYBA_MOUSE
AccessioniPrimary (citable) accession number: P51960
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: November 1, 1997
Last modified: May 11, 2016
This is version 131 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.