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P51957 (NEK4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 133. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Serine/threonine-protein kinase Nek4

EC=2.7.11.1
Alternative name(s):
Never in mitosis A-related kinase 4
Short name=NimA-related protein kinase 4
Serine/threonine-protein kinase 2
Serine/threonine-protein kinase NRK2
Gene names
Name:NEK4
Synonyms:STK2
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length841 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Seems to act exclusively upon threonine residues By similarity.

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Cofactor

Magnesium By similarity.

Subcellular location

Nucleus Probable.

Tissue specificity

Highest expression in adult heart, followed by pancreas, skeletal muscle, brain, liver, kidney, lung and placenta. Present in most primary carcinomas.

Sequence similarities

Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.

Contains 1 protein kinase domain.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P51957-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P51957-2)

The sequence of this isoform differs from the canonical sequence as follows:
     457-502: Missing.
     813-841: RLREHMGEKYTTYSVKARQLKFFEENMNF → SVSLTVSRCLCYRIF
Note: No experimental confirmation available.
Isoform 3 (identifier: P51957-3)

The sequence of this isoform differs from the canonical sequence as follows:
     32-120: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 841841Serine/threonine-protein kinase Nek4
PRO_0000086425

Regions

Domain6 – 261256Protein kinase
Nucleotide binding12 – 209ATP By similarity

Sites

Active site1311Proton acceptor By similarity
Binding site351ATP By similarity

Amino acid modifications

Modified residue1651Phosphothreonine; by autocatalysis By similarity
Modified residue5631Phosphoserine Ref.7

Natural variations

Alternative sequence32 – 12089Missing in isoform 3.
VSP_043334
Alternative sequence457 – 50246Missing in isoform 2.
VSP_037123
Alternative sequence813 – 84129RLREH…ENMNF → SVSLTVSRCLCYRIF in isoform 2.
VSP_037124
Natural variant2251P → A. Ref.1 Ref.2 Ref.5 Ref.8
Corresponds to variant rs1029871 [ dbSNP | Ensembl ].
VAR_040915
Natural variant2391R → G. Ref.8
Corresponds to variant rs35778416 [ dbSNP | Ensembl ].
VAR_040916
Natural variant2501P → L. Ref.8
Corresponds to variant rs56408749 [ dbSNP | Ensembl ].
VAR_040917
Natural variant3571T → I. Ref.8
Corresponds to variant rs2230537 [ dbSNP | Ensembl ].
VAR_040918
Natural variant4561Q → E. Ref.8
Corresponds to variant rs56019351 [ dbSNP | Ensembl ].
VAR_040919
Natural variant5671F → L. Ref.8
Corresponds to variant rs34986855 [ dbSNP | Ensembl ].
VAR_040920
Natural variant7771R → K in a colorectal adenocarcinoma sample; somatic mutation. Ref.8
VAR_040921

Experimental info

Sequence conflict631P → L in BAG35330. Ref.3
Sequence conflict641N → D in ABQ59054. Ref.2
Sequence conflict3331R → K in BAG35330. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified May 5, 2009. Version 2.
Checksum: 43E8F11BA4007263

FASTA84194,597
        10         20         30         40         50         60 
MPLAAYCYLR VVGKGSYGEV TLVKHRRDGK QYVIKKLNLR NASSRERRAA EQEAQLLSQL 

        70         80         90        100        110        120 
KHPNIVTYKE SWEGGDGLLY IVMGFCEGGD LYRKLKEQKG QLLPENQVVE WFVQIAMALQ 

       130        140        150        160        170        180 
YLHEKHILHR DLKTQNVFLT RTNIIKVGDL GIARVLENHC DMASTLIGTP YYMSPELFSN 

       190        200        210        220        230        240 
KPYNYKSDVW ALGCCVYEMA TLKHAFNAKD MNSLVYRIIE GKLPPMPRDY SPELAELIRT 

       250        260        270        280        290        300 
MLSKRPEERP SVRSILRQPY IKRQISFFLE ATKIKTSKNN IKNGDSQSKP FATVVSGEAE 

       310        320        330        340        350        360 
SNHEVIHPQP LSSEGSQTYI MGEGKCLSQE KPRASGLLKS PASLKAHTCK QDLSNTTELA 

       370        380        390        400        410        420 
TISSVNIDIL PAKGRDSVSD GFVQENQPRY LDASNELGGI CSISQVEEEM LQDNTKSSAQ 

       430        440        450        460        470        480 
PENLIPMWSS DIVTGEKNEP VKPLQPLIKE QKPKDQSLAL SPKLECSGTI LAHSNLRLLG 

       490        500        510        520        530        540 
SSDSPASASR VAGITGVCHH AQDQVAGECI IEKQGRIHPD LQPHNSGSEP SLSRQRRQKR 

       550        560        570        580        590        600 
REQTEHRGEK RQVRRDLFAF QESPPRFLPS HPIVGKVDVT STQKEAENQR RVVTGSVSSS 

       610        620        630        640        650        660 
RSSEMSSSKD RPLSARERRR LKQSQEEMSS SGPSVRKASL SVAGPGKPQE EDQPLPARRL 

       670        680        690        700        710        720 
SSDCSVTQER KQIHCLSEDE LSSSTSSTDK SDGDYGEGKG QTNEINALVQ LMTQTLKLDS 

       730        740        750        760        770        780 
KESCEDVPVA NPVSEFKLHR KYRDTLILHG KVAEEAEEIH FKELPSAIMP GSEKIRRLVE 

       790        800        810        820        830        840 
VLRTDVIRGL GVQLLEQVYD LLEEEDEFDR EVRLREHMGE KYTTYSVKAR QLKFFEENMN 


F 

« Hide

Isoform 2 [UniParc].

Checksum: B251D19895A2AD44
Show »

FASTA78188,094
Isoform 3 [UniParc].

Checksum: 9518EF5A24A1795A
Show »

FASTA75284,348

References

« Hide 'large scale' references
[1]"Two novel human serine/threonine kinases with homologies to the cell cycle regulating Xenopus MO15, and NIMA kinases: cloning and characterization of their expression pattern."
Levedakou E.N., He M., Baptist E.W., Craven R.J., Cance W.G., Welcsh P.L., Simmons A., Naylor S.L., Leach R.J., Lewis T.B., Bowcock A., Liu E.T.
Oncogene 9:1977-1988(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT ALA-225.
Tissue: Mammary gland.
[2]Schupp I.
Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT ALA-225.
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
Tissue: Amygdala and Brain.
[4]"The DNA sequence, annotation and analysis of human chromosome 3."
Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J. expand/collapse author list , Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P., Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H., Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J., Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W., Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B., Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H., Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J., Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X., Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R., Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A.
Nature 440:1194-1198(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANT ALA-225.
Tissue: Brain.
[6]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[7]"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-563, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Leukemic T-cell.
[8]"Patterns of somatic mutation in human cancer genomes."
Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G. expand/collapse author list , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R.
Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANTS [LARGE SCALE ANALYSIS] ALA-225; GLY-239; LEU-250; ILE-357; GLU-456; LEU-567 AND LYS-777.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L20321 mRNA. Translation: AAA36658.1.
EF560744 mRNA. Translation: ABQ59054.1.
AK294165 mRNA. Translation: BAH11686.1.
AK312420 mRNA. Translation: BAG35330.1.
AC006254 Genomic DNA. No translation available.
AC104446 Genomic DNA. No translation available.
BC063044 mRNA. Translation: AAH63044.1.
CCDSCCDS2863.1. [P51957-1]
CCDS54593.1. [P51957-3]
PIRI78885.
RefSeqNP_001180462.1. NM_001193533.1. [P51957-3]
NP_003148.2. NM_003157.4. [P51957-1]
UniGeneHs.631921.

3D structure databases

ProteinModelPortalP51957.
SMRP51957. Positions 1-322.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid112663. 2 interactions.
IntActP51957. 1 interaction.
STRING9606.ENSP00000233027.

Chemistry

BindingDBP51957.
ChEMBLCHEMBL5819.
GuidetoPHARMACOLOGY2119.

PTM databases

PhosphoSiteP51957.

Polymorphism databases

DMDM229462924.

Proteomic databases

MaxQBP51957.
PaxDbP51957.
PRIDEP51957.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000233027; ENSP00000233027; ENSG00000114904. [P51957-1]
ENST00000383721; ENSP00000373227; ENSG00000114904. [P51957-2]
ENST00000535191; ENSP00000437703; ENSG00000114904. [P51957-3]
GeneID6787.
KEGGhsa:6787.
UCSCuc003dfq.4. human. [P51957-1]
uc003dfr.3. human. [P51957-2]
uc011bej.2. human. [P51957-3]

Organism-specific databases

CTD6787.
GeneCardsGC03M052719.
H-InvDBHIX0030800.
HGNCHGNC:11399. NEK4.
HPAHPA015750.
MIM601959. gene.
neXtProtNX_P51957.
PharmGKBPA31548.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0515.
HOGENOMHOG000294177.
HOVERGENHBG031740.
InParanoidP51957.
KOK08857.
OMAELPSAIM.
OrthoDBEOG7D59P5.
PhylomeDBP51957.
TreeFamTF106472.

Enzyme and pathway databases

SignaLinkP51957.

Gene expression databases

ArrayExpressP51957.
BgeeP51957.
CleanExHS_NEK4.
GenevestigatorP51957.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSNEK4. human.
GenomeRNAi6787.
NextBio26500.
PMAP-CutDBP51957.
PROP51957.
SOURCESearch...

Entry information

Entry nameNEK4_HUMAN
AccessionPrimary (citable) accession number: P51957
Secondary accession number(s): A5YM70 expand/collapse secondary AC list , B2R633, B7Z200, Q6P576
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: May 5, 2009
Last modified: July 9, 2014
This is version 133 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 3

Human chromosome 3: entries, gene names and cross-references to MIM