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Protein

Serine/threonine-protein kinase Nek1

Gene

Nek1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Phosphorylates serines and threonines, but also appears to possess tyrosine kinase activity. Implicated in the control of meiosis. Involved in cilium assembly. In response to injury that includes DNA damage, NEK1 phosphorylates VDAC1 to limit mitochondrial cell death (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei33 – 331ATPPROSITE-ProRule annotation
Active sitei128 – 1281Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 189ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • cellular response to DNA damage stimulus Source: MGI
  • cilium assembly Source: MGI
  • kidney development Source: MGI
  • mitotic nuclear division Source: UniProtKB-KW
  • multicellular organism growth Source: MGI
  • protein phosphorylation Source: MGI
  • regulation of DNA damage checkpoint Source: CACAO
  • response to ionizing radiation Source: MGI
  • spermatogenesis Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase, Tyrosine-protein kinase

Keywords - Biological processi

Cell cycle, Cell division, Cilium biogenesis/degradation, Mitosis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase Nek1 (EC:2.7.11.1)
Alternative name(s):
Never in mitosis A-related kinase 1
Short name:
NimA-related protein kinase 1
Gene namesi
Name:Nek1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:97303. Nek1.

Subcellular locationi

GO - Cellular componenti

  • centrosome Source: UniProtKB
  • cytoplasm Source: MGI
  • nucleus Source: MGI
  • pericentriolar material Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12031203Serine/threonine-protein kinase Nek1PRO_0000086419Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei156 – 1561PhosphothreonineBy similarity
Modified residuei162 – 1621Phosphothreonine; by autocatalysisBy similarity
Modified residuei417 – 4171PhosphoserineBy similarity
Modified residuei437 – 4371PhosphoserineCombined sources
Modified residuei615 – 6151PhosphothreonineBy similarity
Modified residuei618 – 6181PhosphoserineCombined sources
Modified residuei750 – 7501PhosphoserineBy similarity
Modified residuei786 – 7861PhosphoserineBy similarity
Modified residuei820 – 8201PhosphoserineBy similarity
Modified residuei832 – 8321PhosphoserineBy similarity
Modified residuei997 – 9971PhosphoserineBy similarity
Modified residuei1071 – 10711PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP51954.
MaxQBiP51954.
PaxDbiP51954.
PRIDEiP51954.

PTM databases

iPTMnetiP51954.
PhosphoSiteiP51954.

Expressioni

Tissue specificityi

Predominantly in testes (germ cells and Sertoli cells). Lower levels in ovary (oocytes and granulosa cells), thymus and lung.

Developmental stagei

In female, expressed as follicles enter the secondary stage until ovulation occurs. In the male reproductive system, the expression is limited to spermatocytes and spermatids.

Gene expression databases

BgeeiP51954.
ExpressionAtlasiP51954. baseline and differential.
GenevisibleiP51954. MM.

Interactioni

Subunit structurei

Binds to SPERT (PubMed:12204287). Found in a complex with C21orf2, NEK1 and SPATA7 (By similarity). Interacts with NEK1 (By similarity).By similarity1 Publication

Protein-protein interaction databases

BioGridi201727. 5 interactions.
IntActiP51954. 4 interactions.
STRINGi10090.ENSMUSP00000034065.

Structurei

3D structure databases

ProteinModelPortaliP51954.
SMRiP51954. Positions 1-342.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 258255Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0589. Eukaryota.
ENOG410Y7JF. LUCA.
GeneTreeiENSGT00760000118997.
HOGENOMiHOG000082547.
HOVERGENiHBG006460.
InParanoidiP51954.
KOiK08857.
OrthoDBiEOG7D59P5.
TreeFamiTF333575.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P51954-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKYVRLQKI GEGSFGKAVL VKSTEDGRHY VIKEINISRM SDKERQESRR
60 70 80 90 100
EVAVLANMKH PNIVQYKESF EENGSLYIVM DYCEGGDLFK RINAQKGALF
110 120 130 140 150
QEDQILDWFV QICLALKHVH DRKILHRDIK SQNIFLTKDG TVQLGDFGIA
160 170 180 190 200
RVLNSTVELA RTCIGTPYYL SPEICENKPY NNKSDIWALG CVLYELCTLK
210 220 230 240 250
HAFEAGNMKN LVLKIISGSF PPVSPHYSYD LRSLLSQLFK RNPRDRPSVN
260 270 280 290 300
SILEKGFIAK RIEKFLSPQL IAEEFCLKTL SKFGPQPLPG KRPASGQGVS
310 320 330 340 350
SFVPAQKITK PAAKYGVPLT YKKYGDKKLL EKKPPPKHKQ AHQIPVKKMN
360 370 380 390 400
SGEERKKMSE EAAKKRRLEF IEKEKKQKDQ IRFLKAEQMK RQEKQRLERI
410 420 430 440 450
NRAREQGWRN VLRAGGSGEV KASFFGIGGA VSPSPCSPRG QYEHYHAIFD
460 470 480 490 500
QMQRLRAEDN EARWKGGIYG RWLPERQKGH LAVERANQVE EFLQRKREAM
510 520 530 540 550
QNKARAEGHV VYLARLRQIR LQNFNERQQI KAKLRGENKE ADGTKGQEAT
560 570 580 590 600
EETDMRLKKM ESLKAQTNAR AAVLKEQLER KRKEAYEREK KVWEEHLVAR
610 620 630 640 650
VKSSDVPLPL ELLETGGSPS KQQVKPVISV TSALKEVGLD GSLTDTQEEE
660 670 680 690 700
MEKSNSAISS KREILRRLNE NLKAQEDEKE KQHHSGSCET VGHKDEREYE
710 720 730 740 750
TENAISSDRK KWEMGGQLVI PLDAVTLDTS FSATEKHTVG EVIKLDSNGS
760 770 780 790 800
PRKVWGKNPT DSVLKILGEA ELQLQTELLE NTSFKSEVYA EEENYKPLLT
810 820 830 840 850
EEENLQCISK EINPSATVDS TETKSPKFTE VSPQMSEGNV EEPDDLETEV
860 870 880 890 900
LQEPSSTHTD GSLPPVLNDV WTREKEAAKE TELEDKVAVQ QSEVCEDRIP
910 920 930 940 950
GNVDQSCKDQ RDPAVDDSPQ SGCDVEKSVQ PESIFQKVVH SKDLNLVQAV
960 970 980 990 1000
HCSPEEPIPI RSHSDSPPKT KSKNSLLIGL STGLFDANNP KMLRTCSLPD
1010 1020 1030 1040 1050
LSKLFRTLMD VPTVGDVHQD SLEIDELEDE PIKEGPSDSE DTVFEETDTD
1060 1070 1080 1090 1100
LQELQASMEQ LLREQPGDEY SEEEESVLKS SDVEQTARGT DAPDEEDNPS
1110 1120 1130 1140 1150
SESALNEEWH SDNSDAETTS ECEYDSVFNH LEELRLHLEQ EMGFEKFFEV
1160 1170 1180 1190 1200
YEKVKAIHED EDENIEICST IVENILGNEH QHLYAKILHL VMADGAYQED

NDE
Length:1,203
Mass (Da):136,690
Last modified:January 10, 2006 - v2
Checksum:i06393960F2E719DA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY850065 mRNA. Translation: AAB23529.2.
CCDSiCCDS40347.1.
PIRiS25284.
RefSeqiNP_001280566.1. NM_001293637.1.
NP_001280567.1. NM_001293638.1.
NP_001280568.1. NM_001293639.1.
NP_780298.2. NM_175089.4.
UniGeneiMm.486881.

Genome annotation databases

EnsembliENSMUST00000034065; ENSMUSP00000034065; ENSMUSG00000031644.
GeneIDi18004.
KEGGimmu:18004.
UCSCiuc009ltt.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY850065 mRNA. Translation: AAB23529.2.
CCDSiCCDS40347.1.
PIRiS25284.
RefSeqiNP_001280566.1. NM_001293637.1.
NP_001280567.1. NM_001293638.1.
NP_001280568.1. NM_001293639.1.
NP_780298.2. NM_175089.4.
UniGeneiMm.486881.

3D structure databases

ProteinModelPortaliP51954.
SMRiP51954. Positions 1-342.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201727. 5 interactions.
IntActiP51954. 4 interactions.
STRINGi10090.ENSMUSP00000034065.

PTM databases

iPTMnetiP51954.
PhosphoSiteiP51954.

Proteomic databases

EPDiP51954.
MaxQBiP51954.
PaxDbiP51954.
PRIDEiP51954.

Protocols and materials databases

DNASUi18004.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000034065; ENSMUSP00000034065; ENSMUSG00000031644.
GeneIDi18004.
KEGGimmu:18004.
UCSCiuc009ltt.2. mouse.

Organism-specific databases

CTDi4750.
MGIiMGI:97303. Nek1.

Phylogenomic databases

eggNOGiKOG0589. Eukaryota.
ENOG410Y7JF. LUCA.
GeneTreeiENSGT00760000118997.
HOGENOMiHOG000082547.
HOVERGENiHBG006460.
InParanoidiP51954.
KOiK08857.
OrthoDBiEOG7D59P5.
TreeFamiTF333575.

Miscellaneous databases

PROiP51954.
SOURCEiSearch...

Gene expression databases

BgeeiP51954.
ExpressionAtlasiP51954. baseline and differential.
GenevisibleiP51954. MM.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A mammalian dual specificity protein kinase, Nek1, is related to the NIMA cell cycle regulator and highly expressed in meiotic germ cells."
    Letwin K., Mizzen L., Motro B., Ben-David Y., Bernstein A., Pawson T.
    EMBO J. 11:3521-3531(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Blood.
  2. Feige E., Motro B.
    Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION TO C-TERMINUS.
  3. "Nurit, a novel leucine-zipper protein, expressed uniquely in the spermatid flower-like structure."
    Feige E., Chen A., Motro B.
    Mech. Dev. 117:369-377(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SPERT.
    Strain: CD-1.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic brain.
  5. "NIMA-related kinases defective in murine models of polycystic kidney diseases localize to primary cilia and centrosomes."
    Mahjoub M.R., Trapp M.L., Quarmby L.M.
    J. Am. Soc. Nephrol. 16:3485-3489(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-437; SER-618 AND SER-1071, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Lung, Spleen and Testis.

Entry informationi

Entry nameiNEK1_MOUSE
AccessioniPrimary (citable) accession number: P51954
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: January 10, 2006
Last modified: July 6, 2016
This is version 142 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.