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P51953 (CDK7_CARAU) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cyclin-dependent kinase 7

EC=2.7.11.22
EC=2.7.11.23
Alternative name(s):
40 kDa protein kinase
CDC2/CDK2,4-activating kinase
Cell division protein kinase 7
P40 MO15
Gene names
Name:cdk7
Synonyms:mo15
OrganismCarassius auratus (Goldfish)
Taxonomic identifier7957 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeCarassius

Protein attributes

Sequence length344 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Serine/threonine kinase involved in cell cycle control and in RNA polymerase II-mediated RNA transcription. Cyclin-dependent kinases (CDKs) are activated by the binding to a cyclin and mediate the progression through the cell cycle. Each different complex controls a specific transition between 2 subsequent phases in the cell cycle. Required for both activation and complex formation of cdk1/cyclin-B during G2-M transition, and for activation of cdk2/cyclins during G1-S transition (but not complex formation). cdk7 is the catalytic subunit of the CDK-activating kinase (CAK) complex. CAK activates the cyclin-associated kinases cdk1, cdk2, cdk4 and cdk6 by threonine phosphorylation, thus regulating cell cycle progression. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminal domain (CTD) of its large subunit (polr2a), allowing its escape from the promoter and elongation of the transcripts By similarity.

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

Enzyme regulation

Phosphorylation at Thr-169 is required for enzymatic activity.

Subunit structure

Probably associates with cyclin-H (ccnh) and mat1 to form a multimeric active enzyme By similarity.

Subcellular location

Nucleus By similarity.

Post-translational modification

Phosphorylation of Ser-163 during mitosis inactivates the enzyme. Phosphorylation of Thr-169 is required for activity. Phosphorylated at Ser-163 and Thr-169 by CDK2 By similarity.

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.

Contains 1 protein kinase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 344344Cyclin-dependent kinase 7
PRO_0000085794

Regions

Domain12 – 294283Protein kinase
Nucleotide binding18 – 269ATP By similarity

Sites

Active site1361Proton acceptor By similarity
Binding site411ATP By similarity

Amino acid modifications

Modified residue1631Phosphoserine; by CDK1 and CDK2 By similarity
Modified residue1691Phosphothreonine; by CDK2 By similarity

Sequences

Sequence LengthMass (Da)Tools
P51953 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 2AE835FFBEFAA1BA

FASTA34438,510
        10         20         30         40         50         60 
MALDVKSRAK LYEKLDFLGE GQFATVYKAR DKTTNTIVAI KKIKVGHRTE AKDGINRTAL 

        70         80         90        100        110        120 
REIKLLQELS HPNIIGLLDA FGHKSNISLL CFMETDLEVI IKDTSLVLTP ANIKAYILMS 

       130        140        150        160        170        180 
LQGLEYMHNH WILHRDLKPN NLLLDENGVL KLADFGLAKA FGSPNRVYTH QVVTRWYRAP 

       190        200        210        220        230        240 
ELLFGARMYG VGVDMWAVGS ILAELLLRVP FLAGDSDLDQ LTGIFEALGT PTEETWPGMS 

       250        260        270        280        290        300 
NLPDYVSFKL FPGTPLEHIF SAAGDDLLEL LKGLFTFNPC TRTTASQALK MRYFSIRPGP 

       310        320        330        340 
TPGPQLPRPN SSTEALKEKE NLLIGIKRKR DSIEQGTLKK KLVF 

« Hide

References

[1]"A fish homolog of the cdc2-related protein p40 MO15: its cDNA cloning and expression in oocytes."
Onoe S., Yamashita M., Kajiura H., Katsu Y., Jianquao J., Nagahama Y.
Biomed. Res. 14:441-444(1993)
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Oocyte.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D38631 mRNA. Translation: BAA07611.1.

3D structure databases

ProteinModelPortalP51953.
SMRP51953. Positions 13-310.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Phylogenomic databases

HOVERGENHBG014652.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCDK7_CARAU
AccessionPrimary (citable) accession number: P51953
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 14, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families