Cyclin-dependent kinase 7 - Carassius auratus (Goldfish)

Contribute

Send feedback

Read comments (?) or add your own

P51953 (CDK7_CARAU) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 71. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Cyclin-dependent kinase 7
EC=2.7.11.22
EC=2.7.11.23

Alternative name(s):
40 kDa protein kinase
CDC2/CDK2,4-activating kinase
Cell division protein kinase 7
P40 MO15

Gene names

Name:	cdk7
Synonyms:	mo15

Organism

Carassius auratus (Goldfish)

Taxonomic identifier

7957 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Carassius

Protein attributes

Sequence length	344 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Serine/threonine kinase involved in cell cycle control and in RNA polymerase II-mediated RNA transcription. Cyclin-dependent kinases (CDKs) are activated by the binding to a cyclin and mediate the progression through the cell cycle. Each different complex controls a specific transition between 2 subsequent phases in the cell cycle. Required for both activation and complex formation of cdk1/cyclin-B during G2-M transition, and for activation of cdk2/cyclins during G1-S transition (but not complex formation). cdk7 is the catalytic subunit of the CDK-activating kinase (CAK) complex. CAK activates the cyclin-associated kinases cdk1, cdk2, cdk4 and cdk6 by threonine phosphorylation, thus regulating cell cycle progression. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminus domain (CTD) of its large subunit (polr2a), allowing its escape from the promoter and elongation of the transcripts By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein. ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.
Enzyme regulation	Phosphorylation at Thr-169 is required for enzymatic activity.
Subunit structure	Probably associates with cyclin-H (ccnh) and mat1 to form a multimeric active enzyme By similarity.
Subcellular location	Nucleus By similarity.
Post-translational modification	Phosphorylation of Ser-163 during mitosis inactivates the enzyme. Phosphorylation of Thr-169 is required for activity. Phosphorylated at Ser-163 and Thr-169 by CDK2 By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Meiosis
Cellular component	Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological_process	cell division Inferred from electronic annotation. Source: UniProtKB-KW meiosis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA polymerase II carboxy-terminal domain kinase activity Inferred from electronic annotation. Source: EC cyclin-dependent protein serine/threonine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 344

344

Cyclin-dependent kinase 7

PRO_0000085794

Regions

Domain

12 – 294

283

Protein kinase

Nucleotide binding

18 – 26

ATP By similarity

Sites

Active site

136

Proton acceptor By similarity

Binding site

ATP By similarity

Amino acid modifications

Modified residue

163

Phosphoserine; by CDK1 and CDK2 By similarity

Modified residue

169

Phosphothreonine; by CDK2 By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

P51953 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 2AE835FFBEFAA1BA
Show »

FASTA

344

38,510

        10         20         30         40         50         60 
MALDVKSRAK LYEKLDFLGE GQFATVYKAR DKTTNTIVAI KKIKVGHRTE AKDGINRTAL 

        70         80         90        100        110        120 
REIKLLQELS HPNIIGLLDA FGHKSNISLL CFMETDLEVI IKDTSLVLTP ANIKAYILMS 

       130        140        150        160        170        180 
LQGLEYMHNH WILHRDLKPN NLLLDENGVL KLADFGLAKA FGSPNRVYTH QVVTRWYRAP 

       190        200        210        220        230        240 
ELLFGARMYG VGVDMWAVGS ILAELLLRVP FLAGDSDLDQ LTGIFEALGT PTEETWPGMS 

       250        260        270        280        290        300 
NLPDYVSFKL FPGTPLEHIF SAAGDDLLEL LKGLFTFNPC TRTTASQALK MRYFSIRPGP 

       310        320        330        340 
TPGPQLPRPN SSTEALKEKE NLLIGIKRKR DSIEQGTLKK KLVF

« Hide

References

[1]	"A fish homolog of the cdc2-related protein p40 MO15: its cDNA cloning and expression in oocytes." Onoe S., Yamashita M., Kajiura H., Katsu Y., Jianquao J., Nagahama Y. Biomed. Res. 14:441-444(1993) Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Oocyte.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D38631 mRNA. Translation: BAA07611.1.
3D structure databases
ProteinModelPortal	P51953.
SMR	P51953. Positions 13-310.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG014652.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR002290. Ser/Thr_dual-sp_kinase_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CDK7_CARAU

Accession

Primary (citable) accession number: P51953

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 1, 2013
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections