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P51952

- CDK7_RAT

UniProt

P51952 - CDK7_RAT

Protein

Cyclin-dependent kinase 7

Gene

Cdk7

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 122 (01 Oct 2014)
      Sequence version 2 (10 May 2005)
      Previous versions | rss
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    Functioni

    Serine/threonine kinase involved in cell cycle control and in RNA polymerase II-mediated RNA transcription. Cyclin-dependent kinases (CDKs) are activated by the binding to a cyclin and mediate the progression through the cell cycle. Each different complex controls a specific transition between 2 subsequent phases in the cell cycle. Required for both activation and complex formation of CDK1/cyclin-B during G2-M transition, and for activation of CDK2/cyclins during G1-S transition (but not complex formation). CDK7 is the catalytic subunit of the CDK-activating kinase (CAK) complex. Phosphorylates SPT5/SUPT5H, SF1/NR5A1, POLR2A, p53/TP53, CDK1, CDK2, CDK4, CDK6 and CDK11B/CDK11. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation, thus regulating cell cycle progression. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminal domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Phosphorylation of POLR2A in complex with DNA promotes transcription initiation by triggering dissociation from DNA. Its expression and activity are constant throughout the cell cycle. Upon DNA damage, triggers p53/TP53 activation by phosphorylation, but is inactivated in turn by p53/TP53; this feedback loop may lead to an arrest of the cell cycle and of the transcription, helping in cell recovery, or to apoptosis. Required for DNA-bound peptides-mediated transcription and cellular growth inhibition By similarity.By similarity

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.
    ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

    Enzyme regulationi

    Phosphorylation at Thr-162 is required for enzymatic activity. The association of p53/TP53 to the CAK complex in response to DNA damage reduces kinase activity toward CDK2 and RNA polymerase II repetitive C-terminal domain (CTD), thus stopping cell cycle progression By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei33 – 331ATPPROSITE-ProRule annotation
    Active sitei129 – 1291Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi10 – 189ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. cyclin-dependent protein serine/threonine kinase activity Source: RGD
    3. DNA-dependent ATPase activity Source: UniProtKB
    4. protein complex binding Source: RGD
    5. RNA polymerase II carboxy-terminal domain kinase activity Source: UniProtKB

    GO - Biological processi

    1. ATP catabolic process Source: GOC
    2. DNA repair Source: UniProtKB-KW
    3. meiotic nuclear division Source: UniProtKB-KW
    4. protein phosphorylation Source: RGD
    5. regulation of cell cycle Source: GOC
    6. regulation of transcription, DNA-templated Source: UniProtKB-KW
    7. transcription from RNA polymerase II promoter Source: UniProtKB

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Cell cycle, Cell division, DNA damage, DNA repair, Meiosis, Transcription, Transcription regulation

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cyclin-dependent kinase 7 (EC:2.7.11.22, EC:2.7.11.23)
    Alternative name(s):
    39 protein kinase
    Short name:
    P39 Mo15
    CDK-activating kinase 1
    Cell division protein kinase 7
    TFIIH basal transcription factor complex kinase subunit
    Gene namesi
    Name:Cdk7
    Synonyms:Cak, Cak1, Mo15
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi621124. Cdk7.

    Subcellular locationi

    Nucleus By similarity. Cytoplasm By similarity. Cytoplasmperinuclear region By similarity
    Note: Colocalizes with PRKCI in the cytoplasm and nucleus. Translocates from the nucleus to cytoplasm and perinuclear region in response to DNA-bound peptides By similarity.By similarity

    GO - Cellular componenti

    1. cyclin-dependent protein kinase holoenzyme complex Source: RGD
    2. cytoskeleton Source: RGD
    3. cytosol Source: RGD
    4. holo TFIIH complex Source: UniProtKB
    5. membrane Source: RGD
    6. nucleus Source: RGD
    7. perinuclear region of cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini‹1 – ›329›329Cyclin-dependent kinase 7PRO_0000085793Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei156 – 1561Phosphoserine; by CDK1 and CDK2By similarity
    Modified residuei162 – 1621Phosphothreonine; by CDK2By similarity
    Modified residuei313 – 3131PhosphoserineBy similarity

    Post-translational modificationi

    Phosphorylation of Ser-156 during mitosis inactivates the enzyme. Phosphorylation of Thr-162 is required for activity. Phosphorylated at Ser-156 and Thr-162 by CDK2 By similarity.By similarity

    Keywords - PTMi

    Phosphoprotein

    PTM databases

    PhosphoSiteiP51952.

    Expressioni

    Gene expression databases

    GenevestigatoriP51952.

    Interactioni

    Subunit structurei

    Associates primarily with cyclin-H (CCNH) and MAT1 to form the CAK complex. CAK can further associate with the core-TFIIH to form the TFIIH basal transcription factor; this complex is sensitive to UV light. The CAK complex binds to p53/TP53 in response to DNA damage. Interacts with CDK2, SF1/NR5A1, PUF60 and PRKCI By similarity.By similarity

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000025026.

    Structurei

    3D structure databases

    ProteinModelPortaliP51952.
    SMRiP51952. Positions 5-303.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini4 – 287284Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    HOGENOMiHOG000233024.
    HOVERGENiHBG014652.
    InParanoidiP51952.
    PhylomeDBiP51952.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Fragment.

    P51952-1 [UniParc]FASTAAdd to Basket

    « Hide

    ANRNEKLDFL GEGQFATVYK ARDKNTNQIV AIKKIKLGHR SEAKDGINRT    50
    ALREIKLLQE LSHPNIIGLL DAFGHKSNIS LVFDFMETDL EVIIKDNSLV 100
    LTPSHIKAYM LMTLQGLEYL HQHWILHRDL KPNNLLLDEN GVLKLADFGL 150
    AKSFGSPNWA YTHQVVTRWY RAPELLFGAR MYGVGVDMWA VGCILAELLL 200
    RVPFLPGDSD LDQLTRIFET LGTPTEEQWP DMCSLPDYVT FKSFPGIPLQ 250
    HIFIAAGDDL LELIQGLFLF NPCTRITASQ ALRTKYFSNR PGPTPGCQLP 300
    RPNCPVEALK EQSNPAMATK RKRAEALEQ 329
    Length:329
    Mass (Da):37,141
    Last modified:May 10, 2005 - v2
    Checksum:iB7159FD074881B0F
    GO

    Sequence cautioni

    The sequence CAA58562.1 differs from that shown. Reason: Erroneous termination at position 313. Translated as Ser.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Non-terminal residuei1 – 11
    Non-terminal residuei329 – 3291

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X83579 mRNA. Translation: CAA58562.1. Sequence problems.
    PIRiS51085.
    UniGeneiRn.98896.

    Genome annotation databases

    UCSCiRGD:621124. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X83579 mRNA. Translation: CAA58562.1 . Sequence problems.
    PIRi S51085.
    UniGenei Rn.98896.

    3D structure databases

    ProteinModelPortali P51952.
    SMRi P51952. Positions 5-303.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000025026.

    PTM databases

    PhosphoSitei P51952.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    UCSCi RGD:621124. rat.

    Organism-specific databases

    RGDi 621124. Cdk7.

    Phylogenomic databases

    eggNOGi COG0515.
    HOGENOMi HOG000233024.
    HOVERGENi HBG014652.
    InParanoidi P51952.
    PhylomeDBi P51952.

    Miscellaneous databases

    PROi P51952.

    Gene expression databases

    Genevestigatori P51952.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Wu L., Hall F.
      Submitted (DEC-1994) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Sprague-Dawley.
      Tissue: Testis.

    Entry informationi

    Entry nameiCDK7_RAT
    AccessioniPrimary (citable) accession number: P51952
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: May 10, 2005
    Last modified: October 1, 2014
    This is version 122 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3