Reviewed, UniProtKB/Swiss-Prot P51952 (CDK7_RAT)

Names and origin

Protein names

Recommended name:
    Cell division protein kinase 7
    EC=2.7.11.22
    EC=2.7.11.23
Alternative name(s):
    CDK-activating kinase
      Short name=CAK
    TFIIH basal transcription factor complex kinase subunit
    39 protein kinase
    P39 Mo15

Gene names

Name:	Cdk7
Synonyms:	Cak1

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	329 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Cyclin-dependent kinases (CDKs) are activated by the binding to a cyclin and mediate the progression through the cell cycle. Each different complex controls a specific transition between two subsequent phases in the cell cycle. CDK7 is the catalytic subunit of the CDK-activating kinase (CAK) complex, a serine-threonine kinase. CAK activates the cyclin-associated kinases CDC2/CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminus domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein. ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.
Enzyme regulation	Phosphorylation at Thr-162 is required for enzymatic activity By similarity.
Subunit structure	Associates primarily with cyclin H and MAT1 to form the CAK complex. CAK can further associate with the core-TFIIH to form the TFIIH basal transcription factor. Interacts with PUF60 By similarity.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. Contains 1 protein kinase domain.
Sequence caution	The sequence CAA58562.1 differs from that shown. Reason: Erroneous termination at position 313. Translated as Ser.

Ontologies

Keywords
Biological process	Cell cycle Cell division Meiosis Transcription Transcription regulation
Cellular component	Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	cell division Inferred from electronic annotation. Source: UniProtKB-KW meiosis Inferred from electronic annotation. Source: UniProtKB-KW protein amino acid phosphorylation Inferred from direct assay. Source: RGD regulation of transcription Inferred from electronic annotation. Source: UniProtKB-KW transcription from RNA polymerase II promoter Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	cyclin-dependent protein kinase holoenzyme complex Traceable author statement. Source: RGD holo TFIIH complex Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA-dependent ATPase activity Inferred from sequence or structural similarity. Source: UniProtKB RNA polymerase II carboxy-terminal domain kinase activity Inferred from sequence or structural similarity. Source: UniProtKB cyclin-dependent protein kinase activity Inferred from direct assay. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – ›329	›329	Cell division protein kinase 7		PRO_0000085793
Regions
	Domain	4 – 287	284	Protein kinase
	Nucleotide binding	10 – 18	9	ATP By similarity
Sites
	Active site	129	1	Proton acceptor By similarity
	Binding site	33	1	ATP By similarity
Amino acid modifications
	Modified residue	156	1	Phosphoserine By similarity
	Modified residue	162	1	Phosphothreonine By similarity
	Modified residue	313	1	Phosphoserine By similarity
Experimental info
	Non-terminal residue	1	1
	Non-terminal residue	329	1

Sequences

	Sequence		Length	Mass (Da)
	P51952-1 [UniParc]. Last modified May 10, 2005. Version 2. Checksum: B7159FD074881B0F	FASTA	329	37,141
	10 20 30 40 50 60 ANRNEKLDFL GEGQFATVYK ARDKNTNQIV AIKKIKLGHR SEAKDGINRT ALREIKLLQE 70 80 90 100 110 120 LSHPNIIGLL DAFGHKSNIS LVFDFMETDL EVIIKDNSLV LTPSHIKAYM LMTLQGLEYL 130 140 150 160 170 180 HQHWILHRDL KPNNLLLDEN GVLKLADFGL AKSFGSPNWA YTHQVVTRWY RAPELLFGAR 190 200 210 220 230 240 MYGVGVDMWA VGCILAELLL RVPFLPGDSD LDQLTRIFET LGTPTEEQWP DMCSLPDYVT 250 260 270 280 290 300 FKSFPGIPLQ HIFIAAGDDL LELIQGLFLF NPCTRITASQ ALRTKYFSNR PGPTPGCQLP 310 320 RPNCPVEALK EQSNPAMATK RKRAEALEQ

References

[1]	Wu L., Hall F. Submitted (DEC-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Testis.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X83579 mRNA. Translation: CAA58562.1. Sequence problems.
IPI	IPI00207330.
PIR	S51085.
UniGene	Rn.98896
3D structure databases
HSSP	HSSP built from PDB template 1P2A based on UniProtKB P24941.
SMR	P51952. Positions 5-303.
ModBase	Search...
Protein-protein interaction databases
STRING	P51952.
PTM databases
PhosphoSite	P51952.
Genome annotation databases
Ensembl	ENSRNOT00000025026; ENSRNOP00000025026; ENSRNOG00000018510; Rattus norvegicus. [Genome view]
Organism-specific databases
RGD	621124. Cdk7.
Phylogenomic databases
HOVERGEN	P51952.
Enzyme and pathway databases
BRENDA	2.7.11.22. 248. 2.7.11.23. 248.
Gene expression databases
ArrayExpress	P51952.
Genevestigator	P51952.
GermOnline	ENSRNOG00000018510. Rattus norvegicus.
Family and domain databases
InterPro	IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CDK7_RAT

Accession

Primary (citable) accession number: P51952

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	May 10, 2005
Last modified:	October 13, 2009
This is version 84 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)