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P51949

- MAT1_MOUSE

UniProt

P51949 - MAT1_MOUSE

Protein

CDK-activating kinase assembly factor MAT1

Gene

Mnat1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 119 (01 Oct 2014)
      Sequence version 2 (18 Oct 2001)
      Previous versions | rss
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    Functioni

    Stabilizes the cyclin H-CDK7 complex to form a functional CDK-activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminal domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri6 – 5045RING-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. DNA-dependent ATPase activity Source: Ensembl
    2. protein binding Source: MGI
    3. RNA polymerase II carboxy-terminal domain kinase activity Source: Ensembl
    4. zinc ion binding Source: InterPro

    GO - Biological processi

    1. adult heart development Source: MGI
    2. ATP catabolic process Source: GOC
    3. G1/S transition of mitotic cell cycle Source: Ensembl
    4. negative regulation of apoptotic process Source: Ensembl
    5. positive regulation of smooth muscle cell proliferation Source: Ensembl
    6. positive regulation of transcription from RNA polymerase II promoter Source: Ensembl
    7. protein phosphorylation Source: GOC
    8. regulation of transcription, DNA-templated Source: MGI
    9. response to calcium ion Source: MGI
    10. transcription from RNA polymerase II promoter Source: UniProtKB
    11. ventricular system development Source: MGI

    Keywords - Biological processi

    Cell cycle, Transcription, Transcription regulation

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    ReactomeiREACT_203462. Formation of the Early Elongation Complex.
    REACT_206803. Cyclin A/B1 associated events during G2/M transition.
    REACT_223647. Cyclin A:Cdk2-associated events at S phase entry.
    REACT_223654. Formation of transcription-coupled NER (TC-NER) repair complex.
    REACT_224562. Dual incision reaction in TC-NER.
    REACT_226490. RNA Polymerase II Transcription Elongation.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    CDK-activating kinase assembly factor MAT1
    Alternative name(s):
    CDK7/cyclin-H assembly factor
    Menage a trois
    RING finger protein MAT1
    p35
    p36
    Gene namesi
    Name:Mnat1
    Synonyms:Mat1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 12

    Organism-specific databases

    MGIiMGI:106207. Mnat1.

    Subcellular locationi

    Nucleus By similarity

    GO - Cellular componenti

    1. cytoplasm Source: Ensembl
    2. holo TFIIH complex Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 309309CDK-activating kinase assembly factor MAT1PRO_0000055933Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity
    Modified residuei51 – 511PhosphothreonineBy similarity
    Modified residuei279 – 2791PhosphoserineBy similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiP51949.
    PaxDbiP51949.
    PRIDEiP51949.

    PTM databases

    PhosphoSiteiP51949.

    Expressioni

    Gene expression databases

    BgeeiP51949.
    CleanExiMM_MNAT1.
    GenevestigatoriP51949.

    Interactioni

    Subunit structurei

    Associates primarily with CDK7 and cyclin H to form the CAK complex. CAK can further associate with the core-TFIIH to form the TFIIH basal transcription factor.

    Protein-protein interaction databases

    IntActiP51949. 2 interactions.
    MINTiMINT-4101287.
    STRINGi10090.ENSMUSP00000021523.

    Structurei

    3D structure databases

    ProteinModelPortaliP51949.
    SMRiP51949. Positions 1-65.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati142 – 16120UIMAdd
    BLAST

    Sequence similaritiesi

    Contains 1 RING-type zinc finger.PROSITE-ProRule annotation
    Contains 1 UIM (ubiquitin-interacting motif) repeat.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri6 – 5045RING-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    eggNOGiCOG5220.
    GeneTreeiENSGT00390000002319.
    HOGENOMiHOG000189680.
    HOVERGENiHBG001144.
    InParanoidiQ14BS9.
    KOiK10842.
    OMAiETCGPQV.
    OrthoDBiEOG71VST2.
    PhylomeDBiP51949.
    TreeFamiTF106124.

    Family and domain databases

    Gene3Di3.30.40.10. 1 hit.
    InterProiIPR015877. Cdk-activating_kinase_MAT1_cen.
    IPR004575. MAT1/Tfb3.
    IPR003903. Ubiquitin-int_motif.
    IPR001841. Znf_RING.
    IPR013083. Znf_RING/FYVE/PHD.
    IPR017907. Znf_RING_CS.
    [Graphical view]
    PANTHERiPTHR12683. PTHR12683. 1 hit.
    PfamiPF06391. MAT1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF003338. MAT1_metazoa. 1 hit.
    SMARTiSM00184. RING. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00570. cdk7. 1 hit.
    PROSITEiPS50330. UIM. 1 hit.
    PS00518. ZF_RING_1. 1 hit.
    PS50089. ZF_RING_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P51949-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDDQGCPRCK TTKYRNPSLK LMVNVCGHTL CESCVDLLFV RGAGNCPECG    50
    TPLRKSNFRV QLFEDPTVDK EVEIRKKVLK IYNKREEDFP SLREYNDFLE 100
    EVEEIVFNLT NNVDLENTKK KMEIYQKENK DVIQKNKLKL TREQEELEEA 150
    LEVERQEHEQ RRLFIQKEEE LQQALKRKNK QAFLDELESS DLPVALLLAQ 200
    HKDRSTQLEM QLEKPRSMKP VTFSTGIKMG QQISLAPIQK LEEALYEYQP 250
    LQIETCGPQV PEQELLGRLG YLNHVRAASP QDLAGGYTSS LACHRALQDA 300
    FSGLFWQPR 309
    Length:309
    Mass (Da):35,848
    Last modified:October 18, 2001 - v2
    Checksum:iC72CD43175D69499
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti15 – 151R → P in BAB25483. (PubMed:16141072)Curated
    Sequence conflicti74 – 741I → F in BAB25483. (PubMed:16141072)Curated
    Sequence conflicti117 – 1171N → K in BAB25483. (PubMed:16141072)Curated
    Sequence conflicti139 – 1402KL → NV in BAB25483. (PubMed:16141072)Curated
    Sequence conflicti162 – 1621R → Q in AAA91741. (PubMed:7553872)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U35249 mRNA. Translation: AAA91741.1.
    AK008134 mRNA. Translation: BAB25483.1.
    AK008249 mRNA. Translation: BAB25556.1.
    AK155859 mRNA. Translation: BAE33464.1.
    AK157721 mRNA. Translation: BAE34168.1.
    BC089023 mRNA. Translation: AAH89023.1.
    BC115630 mRNA. Translation: AAI15631.1.
    CCDSiCCDS49087.1.
    PIRiA57235.
    RefSeqiNP_032638.2. NM_008612.2.
    UniGeneiMm.246750.

    Genome annotation databases

    EnsembliENSMUST00000021523; ENSMUSP00000021523; ENSMUSG00000021103.
    GeneIDi17420.
    KEGGimmu:17420.
    UCSCiuc007nwe.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U35249 mRNA. Translation: AAA91741.1 .
    AK008134 mRNA. Translation: BAB25483.1 .
    AK008249 mRNA. Translation: BAB25556.1 .
    AK155859 mRNA. Translation: BAE33464.1 .
    AK157721 mRNA. Translation: BAE34168.1 .
    BC089023 mRNA. Translation: AAH89023.1 .
    BC115630 mRNA. Translation: AAI15631.1 .
    CCDSi CCDS49087.1.
    PIRi A57235.
    RefSeqi NP_032638.2. NM_008612.2.
    UniGenei Mm.246750.

    3D structure databases

    ProteinModelPortali P51949.
    SMRi P51949. Positions 1-65.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P51949. 2 interactions.
    MINTi MINT-4101287.
    STRINGi 10090.ENSMUSP00000021523.

    PTM databases

    PhosphoSitei P51949.

    Proteomic databases

    MaxQBi P51949.
    PaxDbi P51949.
    PRIDEi P51949.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000021523 ; ENSMUSP00000021523 ; ENSMUSG00000021103 .
    GeneIDi 17420.
    KEGGi mmu:17420.
    UCSCi uc007nwe.1. mouse.

    Organism-specific databases

    CTDi 4331.
    MGIi MGI:106207. Mnat1.

    Phylogenomic databases

    eggNOGi COG5220.
    GeneTreei ENSGT00390000002319.
    HOGENOMi HOG000189680.
    HOVERGENi HBG001144.
    InParanoidi Q14BS9.
    KOi K10842.
    OMAi ETCGPQV.
    OrthoDBi EOG71VST2.
    PhylomeDBi P51949.
    TreeFami TF106124.

    Enzyme and pathway databases

    Reactomei REACT_203462. Formation of the Early Elongation Complex.
    REACT_206803. Cyclin A/B1 associated events during G2/M transition.
    REACT_223647. Cyclin A:Cdk2-associated events at S phase entry.
    REACT_223654. Formation of transcription-coupled NER (TC-NER) repair complex.
    REACT_224562. Dual incision reaction in TC-NER.
    REACT_226490. RNA Polymerase II Transcription Elongation.

    Miscellaneous databases

    NextBioi 292038.
    PROi P51949.
    SOURCEi Search...

    Gene expression databases

    Bgeei P51949.
    CleanExi MM_MNAT1.
    Genevestigatori P51949.

    Family and domain databases

    Gene3Di 3.30.40.10. 1 hit.
    InterProi IPR015877. Cdk-activating_kinase_MAT1_cen.
    IPR004575. MAT1/Tfb3.
    IPR003903. Ubiquitin-int_motif.
    IPR001841. Znf_RING.
    IPR013083. Znf_RING/FYVE/PHD.
    IPR017907. Znf_RING_CS.
    [Graphical view ]
    PANTHERi PTHR12683. PTHR12683. 1 hit.
    Pfami PF06391. MAT1. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF003338. MAT1_metazoa. 1 hit.
    SMARTi SM00184. RING. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00570. cdk7. 1 hit.
    PROSITEi PS50330. UIM. 1 hit.
    PS00518. ZF_RING_1. 1 hit.
    PS50089. ZF_RING_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Alternative mechanisms of CAK assembly require an assembly factor or an activating kinase."
      Fisher R.P., Jin P., Chamberlin H.M., Morgan D.O.
      Cell 83:47-57(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Embryo and Small intestine.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

    Entry informationi

    Entry nameiMAT1_MOUSE
    AccessioniPrimary (citable) accession number: P51949
    Secondary accession number(s): Q14BS9
    , Q3TZP0, Q9D8A0, Q9D8D2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 18, 2001
    Last modified: October 1, 2014
    This is version 119 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3