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Protein

CDK-activating kinase assembly factor MAT1

Gene

MNAT1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Stabilizes the cyclin H-CDK7 complex to form a functional CDK-activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminal domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri6 – 50RING-typePROSITE-ProRule annotationAdd BLAST45

GO - Molecular functioni

  • cyclin-dependent protein serine/threonine kinase activator activity Source: InterPro
  • protein N-terminus binding Source: UniProtKB
  • zinc ion binding Source: ProtInc

GO - Biological processi

Keywordsi

Biological processCell cycle, Transcription, Transcription regulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-112382 Formation of RNA Pol II elongation complex
R-HSA-113418 Formation of the Early Elongation Complex
R-HSA-167152 Formation of HIV elongation complex in the absence of HIV Tat
R-HSA-167158 Formation of the HIV-1 Early Elongation Complex
R-HSA-167160 RNA Pol II CTD phosphorylation and interaction with CE during HIV infection
R-HSA-167161 HIV Transcription Initiation
R-HSA-167162 RNA Polymerase II HIV Promoter Escape
R-HSA-167172 Transcription of the HIV genome
R-HSA-167200 Formation of HIV-1 elongation complex containing HIV-1 Tat
R-HSA-167246 Tat-mediated elongation of the HIV-1 transcript
R-HSA-427413 NoRC negatively regulates rRNA expression
R-HSA-5696395 Formation of Incision Complex in GG-NER
R-HSA-674695 RNA Polymerase II Pre-transcription Events
R-HSA-6781823 Formation of TC-NER Pre-Incision Complex
R-HSA-6781827 Transcription-Coupled Nucleotide Excision Repair (TC-NER)
R-HSA-6782135 Dual incision in TC-NER
R-HSA-6782210 Gap-filling DNA repair synthesis and ligation in TC-NER
R-HSA-6796648 TP53 Regulates Transcription of DNA Repair Genes
R-HSA-69202 Cyclin E associated events during G1/S transition
R-HSA-69231 Cyclin D associated events in G1
R-HSA-69273 Cyclin A/B1/B2 associated events during G2/M transition
R-HSA-69656 Cyclin A:Cdk2-associated events at S phase entry
R-HSA-72086 mRNA Capping
R-HSA-73762 RNA Polymerase I Transcription Initiation
R-HSA-73772 RNA Polymerase I Promoter Escape
R-HSA-73776 RNA Polymerase II Promoter Escape
R-HSA-73777 RNA Polymerase I Chain Elongation
R-HSA-73779 RNA Polymerase II Transcription Pre-Initiation And Promoter Opening
R-HSA-73863 RNA Polymerase I Transcription Termination
R-HSA-75953 RNA Polymerase II Transcription Initiation
R-HSA-75955 RNA Polymerase II Transcription Elongation
R-HSA-76042 RNA Polymerase II Transcription Initiation And Promoter Clearance
R-HSA-77075 RNA Pol II CTD phosphorylation and interaction with CE
R-HSA-8939236 RUNX1 regulates transcription of genes involved in differentiation of HSCs
SignaLinkiP51948

Names & Taxonomyi

Protein namesi
Recommended name:
CDK-activating kinase assembly factor MAT1
Alternative name(s):
CDK7/cyclin-H assembly factor
Cyclin-G1-interacting protein
Menage a trois
RING finger protein 66
RING finger protein MAT1
p35
p36
Gene namesi
Name:MNAT1
Synonyms:CAP35, MAT1, RNF66
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 14

Organism-specific databases

EuPathDBiHostDB:ENSG00000020426.10
HGNCiHGNC:7181 MNAT1
MIMi602659 gene
neXtProtiNX_P51948

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi4331
OpenTargetsiENSG00000020426
PharmGKBiPA30894

Chemistry databases

ChEMBLiCHEMBL3038473

Polymorphism and mutation databases

BioMutaiMNAT1
DMDMi1708932

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000559321 – 309CDK-activating kinase assembly factor MAT1Add BLAST309

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei51PhosphothreonineCombined sources1
Modified residuei279PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP51948
MaxQBiP51948
PaxDbiP51948
PeptideAtlasiP51948
PRIDEiP51948

PTM databases

iPTMnetiP51948
PhosphoSitePlusiP51948

Expressioni

Tissue specificityi

Highest levels in colon and testis. Moderate levels are present thymus, prostate, ovary, and small intestine. The lowest levels are found in spleen and leukocytes.

Gene expression databases

BgeeiENSG00000020426
CleanExiHS_MNAT1
ExpressionAtlasiP51948 baseline and differential
GenevisibleiP51948 HS

Organism-specific databases

HPAiCAB004495
HPA000701
HPA001154

Interactioni

Subunit structurei

Associates primarily with CDK7 and cyclin H to form the CAK complex. CAK can further associate with the core-TFIIH to form the TFIIH basal transcription factor.1 Publication

Binary interactionsi

Show more details

GO - Molecular functioni

  • protein N-terminus binding Source: UniProtKB

Protein-protein interaction databases

BioGridi110474, 62 interactors
CORUMiP51948
IntActiP51948, 35 interactors
MINTiP51948
STRINGi9606.ENSP00000261245

Chemistry databases

BindingDBiP51948

Structurei

Secondary structure

1309
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni8 – 11Combined sources4
Helixi12 – 15Combined sources4
Beta strandi21 – 23Combined sources3
Helixi32 – 40Combined sources9
Beta strandi43 – 45Combined sources3
Turni47 – 49Combined sources3
Beta strandi59 – 61Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1G25NMR-A1-65[»]
ProteinModelPortaliP51948
SMRiP51948
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP51948

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini142 – 161UIMPROSITE-ProRule annotationAdd BLAST20

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri6 – 50RING-typePROSITE-ProRule annotationAdd BLAST45

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG3800 Eukaryota
COG5220 LUCA
GeneTreeiENSGT00390000002319
HOGENOMiHOG000189680
HOVERGENiHBG001144
InParanoidiP51948
KOiK10842
OMAiPMVEKEV
OrthoDBiEOG091G0D6M
PhylomeDBiP51948
TreeFamiTF106124

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR015877 Cdk-activating_kinase_MAT1_cen
IPR004575 MAT1/Tfb3
IPR003903 UIM_dom
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
IPR017907 Znf_RING_CS
PANTHERiPTHR12683:SF13 PTHR12683:SF13, 1 hit
PfamiView protein in Pfam
PF06391 MAT1, 1 hit
PF17121 zf-C3HC4_5, 1 hit
PIRSFiPIRSF003338 MAT1_metazoa, 1 hit
SMARTiView protein in SMART
SM00184 RING, 1 hit
TIGRFAMsiTIGR00570 cdk7, 1 hit
PROSITEiView protein in PROSITE
PS50330 UIM, 1 hit
PS00518 ZF_RING_1, 1 hit
PS50089 ZF_RING_2, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P51948-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDDQGCPRCK TTKYRNPSLK LMVNVCGHTL CESCVDLLFV RGAGNCPECG
60 70 80 90 100
TPLRKSNFRV QLFEDPTVDK EVEIRKKVLK IYNKREEDFP SLREYNDFLE
110 120 130 140 150
EVEEIVFNLT NNVDLDNTKK KMEIYQKENK DVIQKNKLKL TREQEELEEA
160 170 180 190 200
LEVERQENEQ RRLFIQKEEQ LQQILKRKNK QAFLDELESS DLPVALLLAQ
210 220 230 240 250
HKDRSTQLEM QLEKPKPVKP VTFSTGIKMG QHISLAPIHK LEEALYEYQP
260 270 280 290 300
LQIETYGPHV PELEMLGRLG YLNHVRAASP QDLAGGYTSS LACHRALQDA

FSGLFWQPS
Length:309
Mass (Da):35,823
Last modified:October 1, 1996 - v1
Checksum:i6818DDE230E81A97
GO
Isoform 2 (identifier: P51948-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     188-229: Missing.

Show »
Length:267
Mass (Da):31,148
Checksum:i078565DB8EA02B92
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti247 – 248EY → DN in AAB05248 (Ref. 3) Curated2

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_052084282D → A. Corresponds to variant dbSNP:rs35188899Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_046772188 – 229Missing in isoform 2. 1 PublicationAdd BLAST42

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X87843 mRNA Translation: CAA61112.1
X92669 mRNA Translation: CAA63356.1
U61835 mRNA Translation: AAB05248.1
AY165512 Genomic DNA Translation: AAN47195.1
AL132777 Genomic DNA No translation available.
AL160236 Genomic DNA No translation available.
CH471061 Genomic DNA Translation: EAW80788.1
CR450336 mRNA Translation: CAG29332.1
CH471061 Genomic DNA Translation: EAW80787.1
BC000820 mRNA Translation: AAH00820.1
CCDSiCCDS53899.1 [P51948-2]
CCDS9750.1 [P51948-1]
PIRiG02764
S60157
RefSeqiNP_001171434.1, NM_001177963.1 [P51948-2]
NP_002422.1, NM_002431.3 [P51948-1]
XP_005267744.1, XM_005267687.2 [P51948-1]
UniGeneiHs.509523

Genome annotation databases

EnsembliENST00000261245; ENSP00000261245; ENSG00000020426 [P51948-1]
ENST00000539616; ENSP00000446437; ENSG00000020426 [P51948-2]
GeneIDi4331
KEGGihsa:4331
UCSCiuc001xfd.4 human [P51948-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiMAT1_HUMAN
AccessioniPrimary (citable) accession number: P51948
Secondary accession number(s): G3V1U8, Q15817, Q6ICQ7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 25, 2018
This is version 188 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health