Reviewed, UniProtKB/Swiss-Prot P51918 (GON1_HAPBU)

Names and origin

Protein names

Recommended name:
    Progonadoliberin-1
Alternative name(s):
    Progonadoliberin I
Cleaved into the following 2 chains:
    1- Recommended name:
            Gonadoliberin-1
        Alternative name(s):
            Gonadoliberin I
            Luteinizing hormone-releasing hormone I
              Short name=LH-RH I
            Gonadotropin-releasing hormone I
              Short name=GnRH-I
            Luliberin I
    2- Recommended name:
            GnRH-associated peptide 1
        Alternative name(s):
            GnRH-associated peptide I

Gene names

Name:

gnrh1

Organism

Haplochromis burtoni (Burton's mouthbrooder)

Taxonomic identifier

8153 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Euteleostei › Neoteleostei › Acanthomorpha › Acanthopterygii › Percomorpha › Perciformes › Labroidei › Cichlidae › African cichlids › Pseudocrenilabrinae › Haplochromini › Haplochromis

Protein attributes

Sequence length	94 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Stimulates the secretion of gonadotropins. May be responsible for the regulation of the hypothalamic-pituitary-gonadal axis.
Subcellular location	Secreted.
Tissue specificity	Synthesized in preoptic neurons and is transported to the pituitary in the preoptic-hypophyseal axons.
Sequence similarities	Belongs to the GnRH family.
Mass spectrometry	Molecular mass is 1113.9 Da from positions 23 - 32. Determined by MALDI. Ref.3

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Hormone
PTM	Amidation Cleavage on pair of basic residues Pyrrolidone carboxylic acid
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	multicellular organismal development Ref.2 Inferred from direct assay. Source: UniProtKB
Cellular component	extracellular region Ref.2 Non-traceable author statement. Source: UniProtKB
Molecular function	gonadotropin hormone-releasing hormone activity Ref.2 Non-traceable author statement. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Ref.3
	Chain	23 – 94	72	Progonadoliberin-1		PRO_0000012432
	Peptide	23 – 32	10	Gonadoliberin-1		PRO_0000012433
	Peptide	36 – 94	59	GnRH-associated peptide 1 Potential		PRO_0000012434
Amino acid modifications
	Modified residue	23	1	Pyrrolidone carboxylic acid
	Modified residue	32	1	Glycine amide
Experimental info
	Sequence conflict	86 – 94	9	ENGHRTFKK → KMDTGHSRNERFL in AAC59691. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P51918-1 [UniParc]. Last modified May 30, 2000. Version 2. Checksum: E57DBA83333278D7	FASTA	94	10,382
	10 20 30 40 50 60 MAAKILALWL LLAGTVFPQG CCQHWSYGLS PGGKRDLDNF SDTLGNMVEE FPRVEAPCSV 70 80 90 FGCAEESPFA KMYRVKGLLA SVAERENGHR TFKK

References

[1]	"Three gonadotropin-releasing hormone genes in one organism suggest novel roles for an ancient peptide." White S.A., Kasten T.L., Bond C.T., Adelman J.P., Fernald R.D. Proc. Natl. Acad. Sci. U.S.A. 92:8363-8367(1995) [PubMed: 7667296] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Ontogeny of gonadotropin-releasing hormone (GnRH) gene expression reveals a distinct origin for GnRH-containing neurons in the midbrain." White R.B., Fernald R.D. Gen. Comp. Endocrinol. 112:322-329(1998) [PubMed: 9843638] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[3]	"Primary structure of solitary form of gonadotropin-releasing hormone (GnRH) in cichlid pituitary; three forms of GnRH in brain of cichlid and pumpkinseed fish." Powell J.F.F., Fischer W.H., Park M., Craig A.G., Rivier J.E., White S.A., Francis R.C., Fernald R.D., Licht P., Warby C., Sherwood N.M. Regul. Pept. 57:43-53(1995) [PubMed: 7644702] [Abstract] Cited for: PROTEIN SEQUENCE OF 23-32, MASS SPECTROMETRY. Tissue: Pituitary.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U31865 mRNA. Translation: AAC59691.1. AF076961 Genomic DNA. Translation: AAC27716.1.
PIR	I50739.
3D structure databases
ModBase	Search...
Phylogenomic databases
HOVERGEN	P51918.
Family and domain databases
InterPro	IPR002012. GnRH. IPR019792. Gonadoliberin_I. IPR004079. Gonadoliberin_I_precursor. [Graphical view]
PANTHER	PTHR10522. GnRH. 1 hit.
Pfam	PF00446. GnRH. 1 hit. [Graphical view]
PRINTS	PR01541. GONADOLIBRNI.
PROSITE	PS00473. GNRH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GON1_HAPBU

Accession

Primary (citable) accession number: P51918
Secondary accession number(s): O93387

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	May 30, 2000
Last modified:	May 5, 2009
This is version 53 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)